##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937582_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050807 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21722521914544 33.0 31.0 34.0 31.0 34.0 2 32.43018675087417 34.0 31.0 34.0 31.0 34.0 3 32.52134501198796 34.0 31.0 34.0 31.0 34.0 4 36.14094597882687 37.0 35.0 37.0 35.0 37.0 5 36.05724673262769 37.0 35.0 37.0 35.0 37.0 6 36.09292829603176 37.0 35.0 37.0 35.0 37.0 7 36.04053575007302 37.0 35.0 37.0 35.0 37.0 8 36.01793440338364 37.0 35.0 37.0 35.0 37.0 9 37.701826646778564 39.0 37.0 39.0 35.0 39.0 10-11 37.635342574898566 39.0 37.0 39.0 35.0 39.0 12-13 37.538655758440456 39.0 37.0 39.0 35.0 39.0 14-15 38.77072879115392 40.0 38.0 41.0 35.0 41.0 16-17 38.72592935366419 40.0 38.0 41.0 35.0 41.0 18-19 38.71233202344248 40.0 38.0 41.0 34.5 41.0 20-21 38.60392494271767 40.0 38.0 41.0 34.0 41.0 22-23 38.45106341064761 40.0 38.0 41.0 34.0 41.0 24-25 38.536419565566135 40.0 38.0 41.0 34.0 41.0 26-27 38.421110567693596 40.0 38.0 41.0 34.0 41.0 28-29 38.276349992953996 40.0 38.0 41.0 34.0 41.0 30-31 38.3128895112997 40.0 38.0 41.0 34.0 41.0 32-33 38.234828777159436 40.0 38.0 41.0 34.0 41.0 34-35 38.10970705678301 40.0 38.0 41.0 34.0 41.0 36-37 37.94735194486853 40.0 37.0 41.0 33.0 41.0 38-39 37.80436603736968 40.0 37.0 41.0 33.0 41.0 40-41 37.62496617185332 40.0 37.0 41.0 33.0 41.0 42-43 37.41351136406303 40.0 37.0 41.0 32.5 41.0 44-45 37.1142077240813 40.0 36.5 41.0 31.5 41.0 46-47 36.773645204058695 40.0 36.0 41.0 31.0 41.0 48-49 36.44990411091828 39.0 36.0 41.0 30.0 41.0 50-51 36.19986571140044 39.0 36.0 41.0 30.0 41.0 52-53 35.950914444899006 39.0 35.0 40.0 29.0 41.0 54-55 35.730981754987184 39.0 35.0 40.0 28.5 41.0 56-57 35.460746184306956 38.5 35.0 40.0 28.0 41.0 58-59 35.18978065707792 38.0 34.5 40.0 27.5 41.0 60-61 34.83029363562734 38.0 34.0 40.0 26.5 41.0 62-63 34.47215437630162 37.5 34.0 40.0 26.0 41.0 64-65 34.074841269802576 37.0 33.5 39.5 26.0 41.0 66-67 33.64006632511007 36.0 33.0 39.0 25.0 40.5 68-69 33.19702219662796 36.0 32.5 39.0 23.5 40.0 70-71 32.77287648228234 35.0 32.0 38.5 23.0 40.0 72-73 32.263722037227296 35.0 31.5 37.5 22.0 40.0 74-75 31.75552550776353 35.0 31.0 37.0 20.5 39.0 76-77 30.531585858640035 33.5 29.5 35.5 20.0 38.5 78-79 31.127669985522772 34.0 31.0 36.0 20.0 38.5 80-81 30.92199875463659 34.0 31.0 36.0 18.5 37.5 82-83 30.544026327197052 34.0 30.5 35.0 18.0 37.0 84-85 30.135294545025445 34.0 30.0 35.0 14.0 36.5 86-87 29.689975946054407 34.0 30.0 35.0 7.5 36.0 88-89 29.2856048862716 34.0 29.0 35.0 4.5 36.0 90-91 28.72050661032462 34.0 29.0 35.0 2.0 35.0 92-93 28.21124172094205 33.0 28.5 35.0 2.0 35.0 94-95 27.702231853119287 33.0 27.0 35.0 2.0 35.0 96-97 27.101464935510755 33.0 26.5 35.0 2.0 35.0 98-99 26.162584777602184 32.5 25.0 34.0 2.0 35.0 100-101 23.435337406201562 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2558073777897292 1101 2 0.2604408284612134 1101 3 0.13860536298489734 1101 4 0.10622813469773007 1101 5 0.09542481651316592 1101 6 0.12337092682139428 1101 7 0.151881492366698 1101 8 0.16097026023331296 1101 9 0.18488605884927267 1101 10-11 0.24609859942199108 1101 12-13 0.2712477447869901 1101 14-15 0.2835526901356431 1101 16-17 0.23113547095111642 1101 18-19 0.24077057299869153 1101 20-21 0.28813304516091875 1101 22-23 0.2638620044416413 1101 24-25 0.23848925354178618 1101 26-27 0.17752403963607577 1101 28-29 0.16292683548182652 1101 30-31 0.3072903656451871 1101 32-33 0.2500655525724298 1101 34-35 0.22858152570461954 1101 36-37 0.24897742456592908 1101 38-39 0.390907582838544 1101 40-41 0.32657554639553155 1101 42-43 0.4164537951959417 1101 44-45 0.2971809903127465 1101 46-47 0.2587889416672482 1101 48-49 0.2794221440794402 1101 50-51 0.23732527139737414 1101 52-53 0.2816295901938375 1101 54-55 0.5237137029318291 1101 56-57 0.44235325293779937 1101 58-59 0.2965200336853826 1101 60-61 0.30452559578035476 1101 62-63 0.31810294091460634 1101 64-65 0.4082841556818906 1101 66-67 0.2513509677314474 1101 68-69 0.15496379636648072 1101 70-71 0.32155553722363805 1101 72-73 0.22306711780712618 1101 74-75 0.13480106060964658 1101 76-77 0.24672818020613363 1101 78-79 0.3038251124211122 1101 80-81 0.3451160970855405 1101 82-83 0.2721799175630153 1101 84-85 0.12115207127898486 1101 86-87 0.02806218331447141 1101 88-89 0.2628898252333407 1101 90-91 0.22661309576261957 1101 92-93 0.3841153479188115 1101 94-95 0.4263805970423675 1101 96-97 0.6150959244491006 1101 98-99 0.5686871380090999 1101 100-101 0.7849394148305926 1102 1 0.2419728747791936 1102 2 0.1826661247425605 1102 3 0.1345498039704509 1102 4 0.10881398650325025 1102 5 0.059886104602583146 1102 6 0.14934324462119974 1102 7 0.10894084924146341 1102 8 0.2006853403075155 1102 9 0.1834324320390479 1102 10-11 0.21904868875066796 1102 12-13 0.19752231223456818 1102 14-15 0.2699505282703498 1102 16-17 0.2544619446887424 1102 18-19 0.2626464092309604 1102 20-21 0.24305319361185695 1102 22-23 0.3879683569000605 1102 24-25 0.23829020706819648 1102 26-27 0.25832247678624753 1102 28-29 0.18432458909136074 1102 30-31 0.2746169540071861 1102 32-33 0.1935207890718047 1102 34-35 0.17383395291710713 1102 36-37 0.26690531675964024 1102 38-39 0.2393211690437056 1102 40-41 0.15738717771899502 1102 42-43 0.21802961242035934 1102 44-45 0.25994455938609917 1102 46-47 0.17223145403575302 1102 48-49 0.07825841799542133 1102 50-51 0.10097833390930333 1102 52-53 0.08055420322317275 1102 54-55 0.23111827298113496 1102 56-57 0.01622990308563743 1102 58-59 0.17832322774734877 1102 60-61 0.02547666477639865 1102 62-63 -0.033697836964371675 1102 64-65 0.11725007059678916 1102 66-67 0.214838257825555 1102 68-69 0.13364438316084915 1102 70-71 0.1887274239038348 1102 72-73 0.09100735892238987 1102 74-75 0.08616517320047379 1102 76-77 0.16470948818576048 1102 78-79 0.2673802288122751 1102 80-81 0.22523342627150456 1102 82-83 0.24292462144247295 1102 84-85 0.07972587185647839 1102 86-87 0.07291629080492967 1102 88-89 0.23752217165625567 1102 90-91 0.38361499613589345 1102 92-93 0.4659302518095707 1102 94-95 0.4025711959465106 1102 96-97 0.4897403517869314 1102 98-99 0.48150478257704066 1102 100-101 0.7643638231440768 1103 1 0.2429251732464479 1103 2 0.22485662976428245 1103 3 0.11194435097910826 1103 4 0.13557668975199277 1103 5 0.13037399871573285 1103 6 0.14058557928632354 1103 7 0.14792721190650582 1103 8 0.15777808195578302 1103 9 0.21411926870832332 1103 10-11 0.15331358556408503 1103 12-13 0.23126395078691075 1103 14-15 0.21753991400748163 1103 16-17 0.29833465480147936 1103 18-19 0.20984261266425364 1103 20-21 0.1687896971183278 1103 22-23 0.21973772988052787 1103 24-25 0.272671905130089 1103 26-27 0.1918479561327473 1103 28-29 0.22730469645461682 1103 30-31 0.19607802997089863 1103 32-33 0.2488971675369882 1103 34-35 0.24528608464106583 1103 36-37 0.2354624692374756 1103 38-39 0.1693579901718607 1103 40-41 0.2614538119004024 1103 42-43 0.3595588507758478 1103 44-45 0.2872679976651824 1103 46-47 0.2579466127818435 1103 48-49 0.2911598565205722 1103 50-51 0.2956404035335325 1103 52-53 0.0839161851048118 1103 54-55 0.12393127280629557 1103 56-57 0.15093804566248537 1103 58-59 0.16564628559270034 1103 60-61 -0.027209966296787513 1103 62-63 0.0027926376226545813 1103 64-65 0.15374985366862148 1103 66-67 0.1655459859724644 1103 68-69 0.21116605169461167 1103 70-71 0.2699459774138546 1103 72-73 0.3164319472445314 1103 74-75 0.13107335106862195 1103 76-77 0.01505313316177137 1103 78-79 0.27041681844245957 1103 80-81 0.1889289190222172 1103 82-83 0.29371980887488647 1103 84-85 0.26142243629709583 1103 86-87 0.29480608320143276 1103 88-89 0.3510361084815905 1103 90-91 0.370263795907956 1103 92-93 0.4189970737795363 1103 94-95 0.5060985450843027 1103 96-97 0.6921960514935961 1103 98-99 0.8237370948370426 1103 100-101 1.0175189947763492 1104 1 0.23459496152424464 1104 2 0.24377637271457786 1104 3 0.10641589214845482 1104 4 0.07293429297041598 1104 5 0.004240811114598841 1104 6 0.024466562944475356 1104 7 0.1020519292510329 1104 8 0.2094022605016761 1104 9 0.18213536937985708 1104 10-11 0.20756259006946465 1104 12-13 0.21097283703027614 1104 14-15 0.19869126254348402 1104 16-17 0.17465310788494293 1104 18-19 0.20162443684827025 1104 20-21 0.14195474567861766 1104 22-23 0.13232043119450765 1104 24-25 0.05466617419415343 1104 26-27 0.11195555764069809 1104 28-29 0.09410982581760408 1104 30-31 0.23783556545463824 1104 32-33 0.1888391868174253 1104 34-35 0.30513795350261574 1104 36-37 0.22816025109758442 1104 38-39 0.24851638243094953 1104 40-41 0.26892803936578247 1104 42-43 0.3317808948239147 1104 44-45 0.2243259475962418 1104 46-47 0.06939477949811135 1104 48-49 0.12767755391757163 1104 50-51 0.2067832082626424 1104 52-53 0.2676039709674214 1104 54-55 0.15698197204718412 1104 56-57 0.23239296019020372 1104 58-59 0.2852012122647736 1104 60-61 0.3550850892422375 1104 62-63 0.3639666798439478 1104 64-65 0.38701074851907435 1104 66-67 0.26012962719073585 1104 68-69 0.38267559869324685 1104 70-71 0.40877556994525577 1104 72-73 0.4136488598770498 1104 74-75 0.29117736486186985 1104 76-77 0.35490261591508343 1104 78-79 0.4401924815068732 1104 80-81 0.3735229342522608 1104 82-83 0.47194242336531644 1104 84-85 0.5287068612535641 1104 86-87 0.6410416126095768 1104 88-89 0.5260042295624707 1104 90-91 0.44590107495775655 1104 92-93 0.4971112229737322 1104 94-95 0.7093628950878923 1104 96-97 0.850506956264784 1104 98-99 1.0325426253056804 1104 100-101 1.162714963536292 1105 1 0.1758675858362011 1105 2 0.13742107160288697 1105 3 0.13311573985919267 1105 4 0.007951257058500971 1105 5 0.037382752945831044 1105 6 0.04591484010673952 1105 7 0.18204044995042779 1105 8 0.061724968499412114 1105 9 0.1466465948650182 1105 10-11 0.1758234411157673 1105 12-13 0.12143045194721935 1105 14-15 0.25427649390655205 1105 16-17 0.25518382304397846 1105 18-19 0.18028718211881767 1105 20-21 0.17709648780911635 1105 22-23 0.22312246702473004 1105 24-25 0.09775754146683369 1105 26-27 0.019149881112582534 1105 28-29 0.06716628735810559 1105 30-31 0.21576514371602684 1105 32-33 0.1905558450002971 1105 34-35 0.10229508096823281 1105 36-37 0.09112749668688735 1105 38-39 0.09016836830107877 1105 40-41 0.21037033947128947 1105 42-43 0.3420571032751738 1105 44-45 0.24480714256071678 1105 46-47 0.2389564084695195 1105 48-49 0.18992971728393826 1105 50-51 0.11965399002117039 1105 52-53 0.09159396358686678 1105 54-55 0.04198856705900056 1105 56-57 -0.07022940363798824 1105 58-59 0.01829874512287688 1105 60-61 -0.010784450321899897 1105 62-63 0.036123021773484254 1105 64-65 0.03944350139361319 1105 66-67 0.14779377804718052 1105 68-69 0.059472538663627006 1105 70-71 -0.0585269270819353 1105 72-73 -0.1382209603853113 1105 74-75 -0.0059214583701709955 1105 76-77 0.07564837943171554 1105 78-79 0.05314768220959465 1105 80-81 -0.07844275632472986 1105 82-83 0.03248386371031131 1105 84-85 0.09583563261229244 1105 86-87 0.35181036964012335 1105 88-89 0.21844625525404027 1105 90-91 0.04625802255307221 1105 92-93 0.349657721472834 1105 94-95 0.508953089642489 1105 96-97 0.6739537070431645 1105 98-99 0.702303955005295 1105 100-101 0.813174100647597 1106 1 0.04272584255451051 1106 2 0.1775082136965338 1106 3 0.15755866719902656 1106 4 0.14506316922061302 1106 5 0.007038189826246821 1106 6 0.045599806168318935 1106 7 0.030428328630492274 1106 8 0.07918204914043514 1106 9 0.07599844280468915 1106 10-11 0.02380980595274451 1106 12-13 -0.0456574953978901 1106 14-15 0.18533434327981269 1106 16-17 0.29066319227437987 1106 18-19 0.12067015961205385 1106 20-21 0.08291838911677019 1106 22-23 0.06450152005989196 1106 24-25 0.1915865084297792 1106 26-27 0.15099776147776822 1106 28-29 0.19110150353191813 1106 30-31 0.2521804726003438 1106 32-33 0.24757105780387434 1106 34-35 0.12418746883406584 1106 36-37 0.1930444349622249 1106 38-39 0.18294672796506717 1106 40-41 0.19220523549650892 1106 42-43 0.053615859081368455 1106 44-45 0.22248223162884528 1106 46-47 0.4558120584364218 1106 48-49 0.5501754734061635 1106 50-51 0.5307474592317263 1106 52-53 0.4388408016962444 1106 54-55 0.4204209235894538 1106 56-57 0.2620882849864259 1106 58-59 0.15513750432896956 1106 60-61 0.13998733341014713 1106 62-63 0.17344236216407438 1106 64-65 0.19630112310234438 1106 66-67 0.23726860535298755 1106 68-69 0.2547009234438562 1106 70-71 0.31938234956802347 1106 72-73 0.3441692601705668 1106 74-75 0.20162080003524707 1106 76-77 0.35120918308205873 1106 78-79 0.22874735239994948 1106 80-81 0.26489395466659715 1106 82-83 0.0609691088796076 1106 84-85 0.21552814466326708 1106 86-87 0.335314451368081 1106 88-89 -0.11637836226386966 1106 90-91 0.020800685931526175 1106 92-93 0.23307626222080913 1106 94-95 0.6480273543841619 1106 96-97 0.6418060379113228 1106 98-99 0.8438107759224636 1106 100-101 0.6460847943325625 1107 1 0.2820660038296552 1107 2 0.31146560939304635 1107 3 0.17962615414237604 1107 4 0.08763874158674412 1107 5 0.1315987706750903 1107 6 0.24269473395275298 1107 7 0.24389751057189102 1107 8 0.15730557860253214 1107 9 0.29680661800876607 1107 10-11 0.2937025182670183 1107 12-13 0.2984594835897596 1107 14-15 0.3782211694405291 1107 16-17 0.35187786452019765 1107 18-19 0.40444737172195744 1107 20-21 0.32702595666180656 1107 22-23 0.4148823522908529 1107 24-25 0.3742376317723952 1107 26-27 0.5604980469949936 1107 28-29 0.4684992203325038 1107 30-31 0.389169401597492 1107 32-33 0.4114740906895449 1107 34-35 0.3914529810856351 1107 36-37 0.45951190656023044 1107 38-39 0.5527594292174527 1107 40-41 0.6993312983202316 1107 42-43 0.7253879343651803 1107 44-45 0.6650677398287996 1107 46-47 0.688280762629816 1107 48-49 0.5951610650671242 1107 50-51 0.705303637954195 1107 52-53 0.7467707838752204 1107 54-55 0.6956803661505404 1107 56-57 0.6568184015199492 1107 58-59 0.7506450170613235 1107 60-61 0.7879975860866821 1107 62-63 0.8542621181344785 1107 64-65 0.7371302242764557 1107 66-67 0.7148445323731849 1107 68-69 0.8429338878357555 1107 70-71 0.8953663628113873 1107 72-73 1.0231277032695658 1107 74-75 0.9790890336565035 1107 76-77 0.9577503012883959 1107 78-79 1.080235202117155 1107 80-81 1.0554141480068822 1107 82-83 1.1521320402707396 1107 84-85 1.125895469680124 1107 86-87 1.1932610070698857 1107 88-89 1.2732484616171256 1107 90-91 1.4100983853168003 1107 92-93 1.4435408204115348 1107 94-95 1.5294940987343288 1107 96-97 1.6356506508540178 1107 98-99 1.6967884487238614 1107 100-101 1.5305691342164671 1108 1 0.11685041778211058 1108 2 0.06962774908178915 1108 3 0.03872254168621225 1108 4 0.01057736726122016 1108 5 -0.06862536532894126 1108 6 -0.017357403344142597 1108 7 0.07428241102194733 1108 8 0.03552876940180738 1108 9 0.06610992630780999 1108 10-11 0.07703835512061374 1108 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68-69 0.003519934545771264 2316 70-71 0.1426478159430289 2316 72-73 0.1319438993849431 2316 74-75 0.2001818940214939 2316 76-77 0.18064884807919768 2316 78-79 0.20287404415125465 2316 80-81 0.17997597499446272 2316 82-83 0.3066647720532778 2316 84-85 0.320158957718256 2316 86-87 0.3613512513222581 2316 88-89 0.42542181268418844 2316 90-91 0.6029786878242085 2316 92-93 0.6202079136183052 2316 94-95 0.8036477397711046 2316 96-97 0.7608448558543621 2316 98-99 0.8148171031708031 2316 100-101 1.3592030943052364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 16.0 5 50.0 6 57.0 7 177.0 8 457.0 9 932.0 10 1468.0 11 2550.0 12 3957.0 13 5917.0 14 7807.0 15 10748.0 16 14574.0 17 20044.0 18 18666.0 19 11662.0 20 9861.0 21 10075.0 22 10703.0 23 11757.0 24 13504.0 25 16311.0 26 19827.0 27 24277.0 28 30825.0 29 38478.0 30 48231.0 31 60924.0 32 78120.0 33 103079.0 34 141330.0 35 202263.0 36 305359.0 37 451790.0 38 344382.0 39 30613.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.649961446114546 19.549087801901795 15.43550283679538 22.36544791518828 2 15.453770149994611 20.10574373892814 40.81924822764892 23.62123788342833 3 19.65221495733143 24.03921968278829 29.996923162442883 26.3116421974374 4 12.961124477155767 16.935910926554588 37.07764084489873 33.025323751390914 5 13.578592870955863 37.443308321282515 34.362217090294564 14.615881717467062 6 32.10107045665438 36.46408462619837 17.08790734574243 14.346937571404816 7 28.67861285825531 32.85794323892984 20.15772327673935 18.30572062607549 8 26.283409409076526 35.99587869555741 19.08648644167881 18.634225453687257 9 25.900291933858234 17.1504193227349 20.406210823349053 36.54307792005781 10-11 24.97473554916078 27.169018627549697 26.766981672819938 21.08926415046958 12-13 25.8101394982549 24.23417372000629 27.862103603938575 22.093583177800234 14-15 22.452966076281193 25.639199593135775 26.434325609382064 25.47350872120097 16-17 21.74139253474364 29.052343784666228 27.1562609255771 22.050002755013026 18-19 21.639310768882687 27.191442198120058 30.00526134346138 21.163985689535874 20-21 22.1573019791721 26.22960132279634 30.5492423226564 21.063854375375158 22-23 21.955812042705595 26.170466431147098 30.309491243596966 21.56423028255034 24-25 21.737897621202986 26.271116397922672 30.53188125348796 21.459104727386386 26-27 21.60215780207681 26.698875384882076 30.32664721237981 21.372319600661307 28-29 21.44316520031188 26.75707792704911 30.39986795248825 21.399888920150755 30-31 21.5959132185525 26.45780417172362 30.40483575490039 21.541446854823494 32-33 21.385605763974866 26.513318903241505 30.353441352599248 21.747633980184386 34-35 21.87058557923783 26.534920155821585 30.20406113300764 21.390433131932944 36-37 21.85761507543128 26.455317345805824 30.093372999019408 21.593694579743484 38-39 21.62023653632682 26.698948291293352 30.207472137167567 21.47334303521226 40-41 21.909828693388153 26.572456116440613 30.077801478596243 21.43991371157499 42-43 21.729426547323445 26.533741396307875 29.97319434937958 21.763637706989098 44-45 21.860502651152768 26.849711771859454 29.655535433385705 21.634250143602078 46-47 21.992245004039873 27.07836476079904 29.169273364095204 21.76011687106588 48-49 22.085549737249778 27.099332116576548 29.03483848065664 21.78027966551704 50-51 22.10627328656475 27.31419875200348 28.84349916886377 21.736028792568 52-53 22.271891016560797 27.387755161748522 28.605324635619052 21.735029186071632 54-55 22.135895771762044 27.444732732041583 28.676662406555074 21.742709089641295 56-57 22.118073519351164 27.67239920675129 28.570143363076095 21.639383910821447 58-59 22.22364171762628 27.319952584519168 28.627120933369156 21.829284764485397 60-61 22.0953751376897 27.361082734747832 28.88289829320848 21.660643834353987 62-63 22.069482891369105 27.414427588749206 28.844791343115173 21.671298176766513 64-65 21.98644241023168 27.332142907645622 28.79027621809366 21.89113846402904 66-67 21.9426791502077 27.449633241938415 28.664130754381077 21.943556853472803 68-69 22.053475560340665 27.317600982247953 28.753719269399447 21.87520418801193 70-71 22.027840747569126 27.438930138233385 28.614296713440122 21.91893240075736 72-73 22.133530849075512 27.383878638994307 28.497074566256114 21.98551594567407 74-75 22.06014510385424 27.594551803265738 28.293886260384326 22.051416832495697 76-77 22.10834564149625 27.375247890220777 28.549639239577395 21.966767228705578 78-79 22.100031841124007 27.470429933192154 28.458333719360233 21.97120450632361 80-81 21.989896165189727 27.67992869143368 28.406131151881542 21.924043991495047 82-83 21.995522626777635 27.726604237656748 28.360663092049954 21.91721004351566 84-85 22.09235675389696 27.602481473670608 28.262127294381823 22.043034478050615 86-87 22.019721992366907 27.615494778396993 28.33255396534145 22.03222926389465 88-89 22.10590757687096 27.5770708799024 28.32928695874356 21.987734584483086 90-91 22.00755104170629 27.528799197104426 28.362734799651612 22.100914961537672 92-93 21.96789866761331 27.40167528462674 28.479229123308674 22.151196924451273 94-95 21.941070027555007 27.398823975147344 28.434733253787414 22.22537274351024 96-97 21.963451460815183 27.297302964150212 28.407573213861664 22.331672361172945 98-99 21.8484102500587 27.23914357401348 28.279992207837168 22.632453968090655 100-101 21.822858447114456 27.33965786655274 28.563036595286444 22.274447091046355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 401.0 1 297.0 2 276.0 3 401.5 4 340.0 5 277.0 6 596.0 7 1473.0 8 2465.5 9 2908.5 10 2644.5 11 2283.0 12 2343.5 13 2537.0 14 2755.5 15 2989.0 16 3225.5 17 3359.0 18 3574.5 19 3938.0 20 4384.5 21 5065.5 22 6000.5 23 7308.5 24 8791.5 25 10882.0 26 13741.0 27 16988.5 28 19791.0 29 22674.5 30 26394.0 31 30024.5 32 33773.5 33 37699.5 34 41655.0 35 45518.0 36 50190.5 37 54400.0 38 57617.5 39 60598.0 40 63611.5 41 66842.5 42 69235.5 43 72552.5 44 76223.5 45 77998.5 46 78342.5 47 79717.0 48 82188.0 49 80506.5 50 76765.0 51 73115.0 52 68794.5 53 65901.5 54 63784.0 55 59308.0 56 52653.5 57 46781.5 58 41919.0 59 36638.5 60 29965.0 61 23962.5 62 19083.5 63 14237.5 64 10155.0 65 7545.0 66 5652.0 67 4224.5 68 3066.5 69 2124.0 70 1489.5 71 1011.0 72 744.5 73 622.0 74 483.5 75 383.0 76 285.0 77 173.0 78 110.5 79 74.0 80 46.5 81 38.5 82 28.0 83 15.5 84 10.0 85 3.0 86 2.5 87 4.5 88 2.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020967355777506122 2 0.0 3 0.0 4 2.4380646252914097E-4 5 0.022527717137692627 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4380646252914098E-5 12-13 0.0031451033666259186 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010727484351282202 24-25 7.314193875874229E-5 26-27 8.045613263461651E-4 28-29 0.0014628387751748457 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.314193875874229E-5 40-41 5.11993571311196E-4 42-43 0.014945336153036342 44-45 4.8761292505828196E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.8761292505828196E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.314193875874229E-5 82-83 0.0024868259177972377 84-85 4.632322788053679E-4 86-87 0.0 88-89 0.0 90-91 0.011166335983834656 92-93 0.0024868259177972377 94-95 0.0 96-97 0.0 98-99 0.0013896968364161034 100-101 0.018480529859708886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.523107989870205 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50356871211712 46.9075685876379 2 13.340826492856136 16.148565650025066 3 4.096657995429679 7.438274227648671 4 1.7974658273048794 4.351528734962984 5 0.9255971669270467 2.8010008644521784 6 0.5395577860506053 1.959342849114964 7 0.3564809985291926 1.5102736579223361 8 0.25485183664419964 1.2339540184506923 9 0.18565804351663093 1.0112941635250565 >10 0.9213510110118179 9.89772773732431 >50 0.05181574728905089 2.1252981455832125 >100 0.024473295672989344 2.7010016910114802 >500 0.0012914945909355295 0.49903637392079714 >1k 1.6143682386693606E-4 0.19170031632551723 >5k 1.6143682386693606E-4 0.7248362877985981 >10k+ 8.071841193346803E-5 0.4985966942961847 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10206 0.49765775131448253 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8830 0.4305622128264629 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6007 0.29290908408251 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2296 0.11195592759338154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 4.8761292505828196E-5 0.0 0.0 0.0 0.0 16-17 4.8761292505828196E-5 0.0 0.0 0.0 0.0 18-19 4.8761292505828196E-5 0.0 0.0 0.0 0.0 20-21 4.8761292505828196E-5 0.0 0.0 9.752258501165639E-5 0.0 22-23 4.8761292505828196E-5 0.0 0.0 1.9504517002331278E-4 0.0 24-25 4.8761292505828196E-5 0.0 0.0 3.4132904754079733E-4 0.0 26-27 4.8761292505828196E-5 0.0 0.0 4.6323227880536784E-4 0.0 28-29 4.8761292505828196E-5 0.0 0.0 0.0010483677888753061 0.0 30-31 4.8761292505828196E-5 0.0 0.0 0.002755013026579293 0.0 32-33 4.8761292505828196E-5 0.0 0.0 0.005217458298123616 0.0 34-35 4.8761292505828196E-5 0.0 0.0 0.00702162612083926 0.0 36-37 4.8761292505828196E-5 0.0 0.0 0.009557213331142326 0.0 38-39 4.8761292505828196E-5 0.0 0.0 0.01350687802411441 0.0 40-41 4.8761292505828196E-5 0.0 0.0 0.018066058873409348 0.0 42-43 1.462838775174846E-4 0.0 0.0 0.022747142953968853 0.0 44-45 2.4380646252914097E-4 0.0 0.0 0.02481949788546655 0.0 46-47 2.4380646252914097E-4 0.0 0.0 0.027696414143310415 0.0 48-49 2.4380646252914097E-4 0.0 0.0 0.031889885298811635 0.0 50-51 2.4380646252914097E-4 0.0 0.0 0.03657096937937114 0.0 52-53 2.4380646252914097E-4 0.0 0.0 0.04763978277819414 0.0 54-55 2.4380646252914097E-4 0.0 0.0 0.055539112164138316 0.0 56-57 2.4380646252914097E-4 0.0 0.0 0.07440973236389382 0.0 58-59 2.4380646252914097E-4 0.0 0.0 0.10183795939842219 0.0 60-61 2.4380646252914097E-4 0.0 0.0 0.12685250245391205 0.0 62-63 2.4380646252914097E-4 0.0 0.0 0.14048128370929103 0.0 64-65 2.4380646252914097E-4 0.0 0.0 0.16388670411208855 0.0 66-67 2.4380646252914097E-4 0.0 0.0 0.2122335256316172 0.0 68-69 2.4380646252914097E-4 0.0 0.0 0.2806943803098 0.0 70-71 2.9256775503496915E-4 0.0 0.0 0.34635146066889766 0.0 72-73 2.9256775503496915E-4 0.0 0.0 0.4217851801754139 0.0 74-75 2.9256775503496915E-4 0.0 0.0 0.5070443001218544 0.0 76-77 2.9256775503496915E-4 0.0 0.0 0.5745299289499207 0.0 78-79 2.9256775503496915E-4 0.0 0.0 0.6600084747126376 0.0 80-81 2.9256775503496915E-4 0.0 0.0 0.7751095056726449 0.0 82-83 2.9256775503496915E-4 0.0 0.0 0.8794586716351174 0.0 84-85 3.1694840128788324E-4 0.0 0.0 1.0013375222534349 0.0 86-87 3.4132904754079733E-4 0.0 0.0 1.1598361035436295 0.0 88-89 3.4132904754079733E-4 0.0 0.0 1.36858319676108 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5780 0.0 45.05454 1 GTATCAA 9295 0.0 37.526016 1 ATCAACG 11785 0.0 29.427408 3 TCAACGC 11920 0.0 29.13398 4 GGACCGA 460 0.0 28.911737 6 TATCAAC 11980 0.0 28.908758 2 CAACGCA 12115 0.0 28.585922 5 AACGCAG 12490 0.0 27.760956 6 AGGACCG 560 0.0 24.601303 5 ACGCAGA 14205 0.0 24.311375 7 CGCAGAG 14365 0.0 23.974453 8 GTACATG 7835 0.0 22.683937 1 GAGTACT 9540 0.0 22.655798 12-13 TACATGG 7805 0.0 22.034054 2 GCAGAGT 15455 0.0 21.853296 9 GTACTTT 10020 0.0 21.355074 14-15 AGTACTT 9935 0.0 21.205187 12-13 ACATGGG 7845 0.0 21.195017 3 ACCGAGT 585 0.0 21.112217 8 AGAGTAC 14210 0.0 19.739775 10-11 >>END_MODULE