FastQCFastQC Report
Thu 26 May 2016
SRR937581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975489
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78760.39868609746751316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60150.3044815739292904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.21311179156148174No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28210.14280008645960568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48500.044.009791
GTATCAA78900.036.271391
TATCAAC96500.029.4515632
ATCAACG96700.029.338523
TCAACGC97750.029.0248494
CAACGCA100400.028.3060875
AACGCAG102650.027.814656
ACGCAGA117600.024.1978937
TACATGG79950.024.075262
CGCAGAG117800.023.955178
GTACATG81000.023.9452741
TAACGCA2452.9649527E-1023.2770294
ACATGGG80150.022.886223
GAGTACT68250.022.0621912-13
GCAGAGT126450.021.5645359
GTACTTT74150.020.2421614-15
AGTACTT73300.019.66738912-13
AGAGTAC114700.019.44402710-11
CAGAGTA122100.019.4132810-11
CATGGGA45350.019.1772444
Produced by FastQC (version 0.11.5)