##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937581_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975489 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.533929573892845 33.0 31.0 34.0 30.0 34.0 2 31.66808724320915 33.0 31.0 34.0 30.0 34.0 3 31.689865142250856 33.0 31.0 34.0 30.0 34.0 4 35.290756870830464 37.0 35.0 37.0 33.0 37.0 5 35.27760367179974 37.0 35.0 37.0 33.0 37.0 6 35.32210910817524 37.0 35.0 37.0 33.0 37.0 7 35.2558601946151 37.0 35.0 37.0 33.0 37.0 8 35.23395270740561 37.0 35.0 37.0 32.0 37.0 9 36.82763001970651 39.0 37.0 39.0 33.0 39.0 10-11 36.700858622852365 39.0 37.0 39.0 32.0 39.0 12-13 36.56016738134204 39.0 37.0 39.0 32.0 39.0 14-15 37.670433244629564 40.0 37.5 41.0 32.0 41.0 16-17 37.59258948037676 40.0 37.0 41.0 32.0 41.0 18-19 37.514770520109195 40.0 37.0 41.0 32.0 41.0 20-21 37.42000309796714 40.0 37.0 41.0 32.0 41.0 22-23 37.209887273480135 39.5 37.0 41.0 31.5 41.0 24-25 37.03188830714826 39.0 36.0 41.0 31.0 41.0 26-27 36.970677386712865 39.0 36.5 41.0 31.0 41.0 28-29 37.02252632133107 39.0 36.5 41.0 31.0 41.0 30-31 36.84270198416696 39.0 36.0 41.0 30.5 41.0 32-33 36.60413219208004 39.0 36.0 41.0 30.0 41.0 34-35 36.61022030494728 39.0 36.0 41.0 30.0 41.0 36-37 36.50196533617752 39.0 36.0 41.0 30.0 41.0 38-39 36.363043023777905 39.0 35.0 41.0 30.0 41.0 40-41 36.116521023402306 39.0 35.0 41.0 29.5 41.0 42-43 35.861149062333425 39.0 35.0 40.0 28.5 41.0 44-45 35.628774698315205 39.0 35.0 40.0 27.0 41.0 46-47 35.297673386184385 38.5 35.0 40.0 26.0 41.0 48-49 34.93406366727428 38.0 34.5 40.0 24.5 41.0 50-51 34.01098487513724 37.5 33.5 39.5 23.5 40.5 52-53 34.112376986153805 38.0 33.5 39.5 23.5 40.5 54-55 34.45409212605082 38.0 34.0 40.0 23.5 41.0 56-57 34.23341435968512 38.0 34.0 40.0 23.0 41.0 58-59 33.969192944126746 38.0 33.5 40.0 22.0 41.0 60-61 33.625761014108406 37.5 33.0 40.0 20.5 41.0 62-63 33.23966091433564 37.0 32.5 40.0 19.5 41.0 64-65 32.85052156706517 36.5 32.0 39.5 18.0 41.0 66-67 32.424334430614394 36.0 31.5 39.0 14.5 40.5 68-69 31.922419714814914 35.5 31.0 39.0 10.0 40.0 70-71 31.462071669343644 35.0 31.0 38.0 8.0 40.0 72-73 30.981552162527862 35.0 30.0 37.5 7.0 40.0 74-75 30.49560691049153 35.0 30.0 37.0 2.0 39.0 76-77 29.97879082090561 34.0 29.0 36.5 2.0 39.0 78-79 29.41650953257649 34.0 29.0 36.0 2.0 38.5 80-81 28.850730882328374 34.0 28.5 35.5 2.0 37.0 82-83 28.285520698925684 34.0 27.0 35.0 2.0 37.0 84-85 27.788455921546515 33.5 26.0 35.0 2.0 36.0 86-87 27.273695019309145 33.0 26.0 35.0 2.0 36.0 88-89 26.742112459244268 33.0 25.0 35.0 2.0 35.5 90-91 26.189382983149997 32.0 24.5 35.0 2.0 35.0 92-93 25.564987959943082 32.0 21.5 34.5 2.0 35.0 94-95 24.866767924296212 31.5 18.5 34.0 2.0 35.0 96-97 24.060637138450275 31.0 6.0 34.0 2.0 35.0 98-99 23.037557536387194 31.0 2.0 34.0 2.0 35.0 100-101 20.63824703655652 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09996314118843941 1101 2 0.29385395174255535 1101 3 0.22129158915754488 1101 4 0.13466895715228588 1101 5 0.19245735392534158 1101 6 0.17781679651869808 1101 7 0.2241804477117526 1101 8 0.2503308643410236 1101 9 0.2859053563591445 1101 10-11 0.1999443784604651 1101 12-13 0.3200562099155988 1101 14-15 0.5556487549996874 1101 16-17 0.5923317555060166 1101 18-19 0.5041313386169151 1101 20-21 0.6603403002602235 1101 22-23 0.3952670104225149 1101 24-25 0.582861256752885 1101 26-27 0.6028975619772083 1101 28-29 0.42962350108948044 1101 30-31 0.5157177628222769 1101 32-33 0.6529597401837037 1101 34-35 0.707868670793971 1101 36-37 0.7295926771217722 1101 38-39 0.7928779486271438 1101 40-41 0.8838883783026361 1101 42-43 0.6628338941011478 1101 44-45 0.7496402858205045 1101 46-47 0.9214874508379793 1101 48-49 0.8436229355825162 1101 50-51 0.7988935327215145 1101 52-53 0.90295044307274 1101 54-55 0.9776477784757205 1101 56-57 0.8808919595380686 1101 58-59 0.9364732373918869 1101 60-61 1.154570566605564 1101 62-63 1.115820097125635 1101 64-65 0.9322118459389159 1101 66-67 1.1292708961658064 1101 68-69 1.0243170900440894 1101 70-71 1.0361080057474545 1101 72-73 1.1848292279989856 1101 74-75 1.211038735774931 1101 76-77 1.3689656459756172 1101 78-79 1.5174871937578551 1101 80-81 1.3659120651296952 1101 82-83 1.3411985317007797 1101 84-85 1.5784481653563383 1101 86-87 1.6205232838265005 1101 88-89 1.5756237425617101 1101 90-91 1.7103036852448703 1101 92-93 1.821737833818517 1101 94-95 1.847374663732193 1101 96-97 1.713232722917848 1101 98-99 1.9815580302961848 1101 100-101 2.4386590547982685 1102 1 0.10598434052105432 1102 2 -0.009758399813044605 1102 3 0.14575404049395146 1102 4 0.06623363474576394 1102 5 0.13233164646305084 1102 6 -0.02123569473003073 1102 7 -0.009348153394014957 1102 8 0.08658155953519042 1102 9 0.281709859581575 1102 10-11 0.08545469187035337 1102 12-13 0.16499131149573287 1102 14-15 0.3511426700060625 1102 16-17 0.19588143735245467 1102 18-19 0.18324484337695424 1102 20-21 0.25858892214002793 1102 22-23 0.3187354363393524 1102 24-25 0.35693756862517034 1102 26-27 0.22195446975356958 1102 28-29 0.14432992468356076 1102 30-31 0.3424825336535591 1102 32-33 0.3414519253834314 1102 34-35 0.4087894963574925 1102 36-37 0.5264767759865663 1102 38-39 0.5152698459347036 1102 40-41 0.4259714458264341 1102 42-43 0.4871393133441231 1102 44-45 0.5488333494185014 1102 46-47 0.6540692588335375 1102 48-49 0.6249203723607408 1102 50-51 0.49941871101837165 1102 52-53 0.35257820749188085 1102 54-55 0.6304037121623765 1102 56-57 0.542570403611748 1102 58-59 0.4400988794241414 1102 60-61 0.5041015150139785 1102 62-63 0.6200563854431067 1102 64-65 0.5700399162434948 1102 66-67 0.680947540913408 1102 68-69 0.6931652351024944 1102 70-71 0.7005874688939819 1102 72-73 0.7228610515341778 1102 74-75 0.89653698552139 1102 76-77 0.6742807120599537 1102 78-79 0.8644829326265686 1102 80-81 0.8399646586128675 1102 82-83 0.641554103068593 1102 84-85 0.769323741958555 1102 86-87 0.8771349892604654 1102 88-89 0.9786561181628493 1102 90-91 1.039809872472926 1102 92-93 0.9614129736752268 1102 94-95 1.1123730688749909 1102 96-97 1.2961312531956004 1102 98-99 1.6817799812563727 1102 100-101 2.0666154702135806 1103 1 0.17327358322364717 1103 2 0.27480860365704984 1103 3 0.20412947425594652 1103 4 0.31314371843631505 1103 5 0.19544911941671472 1103 6 0.15265438487805483 1103 7 0.06873793560374253 1103 8 0.010326201298809679 1103 9 0.11827216821598796 1103 10-11 0.17077439997404298 1103 12-13 0.1647859124347022 1103 14-15 0.19678208621345306 1103 16-17 0.21736222424773644 1103 18-19 0.08290548840655276 1103 20-21 0.09144703093775064 1103 22-23 0.11653154268758215 1103 24-25 0.20399755535714803 1103 26-27 0.20528525154577437 1103 28-29 0.05276914697381585 1103 30-31 0.06844829197158475 1103 32-33 -0.027542384679847487 1103 34-35 0.002424039121315502 1103 36-37 0.0063336345291276075 1103 38-39 0.0600819249304152 1103 40-41 0.11018952604688792 1103 42-43 0.1808178701924561 1103 44-45 0.07818181339074215 1103 46-47 0.15444880057674482 1103 48-49 0.262429302754704 1103 50-51 0.07069537462318465 1103 52-53 -0.0069664699933227325 1103 54-55 0.13487337433833346 1103 56-57 0.0980153923849656 1103 58-59 0.09609500108162194 1103 60-61 0.2695919212193232 1103 62-63 0.4576756699909694 1103 64-65 0.4623642426366317 1103 66-67 0.35828985567152216 1103 68-69 0.40425806016882504 1103 70-71 0.5448273647911073 1103 72-73 0.5763324232426861 1103 74-75 0.502441251698162 1103 76-77 0.6391555589674844 1103 78-79 0.7031451596116689 1103 80-81 0.47189400643895496 1103 82-83 0.746780935075126 1103 84-85 0.7124205474290619 1103 86-87 0.6980474203688551 1103 88-89 0.7991392524759782 1103 90-91 0.7013983344039332 1103 92-93 0.9122294668370969 1103 94-95 0.8990782638127364 1103 96-97 1.4037354836507951 1103 98-99 1.3421653915441851 1103 100-101 1.978287495116625 1104 1 0.24553620566333834 1104 2 0.2784806680330689 1104 3 0.21662440545542694 1104 4 0.2719886627020358 1104 5 0.2585225697154314 1104 6 0.2154137948246131 1104 7 0.18389522611585107 1104 8 0.24188838309078164 1104 9 0.47054073804829244 1104 10-11 0.35956784522650054 1104 12-13 0.39417797176444935 1104 14-15 0.6464099236153444 1104 16-17 0.5706384716232193 1104 18-19 0.5761921437191049 1104 20-21 0.6071265205433249 1104 22-23 0.5230018102301415 1104 24-25 0.5364892425242971 1104 26-27 0.5573445362366414 1104 28-29 0.3833117113813813 1104 30-31 0.4966305975774503 1104 32-33 0.5553786862112702 1104 34-35 0.42568104758283454 1104 36-37 0.6096058268641968 1104 38-39 0.6333229385102968 1104 40-41 0.5707846708118254 1104 42-43 0.5950517378900031 1104 44-45 0.5253117458968433 1104 46-47 0.7241898637182302 1104 48-49 0.5472643527218111 1104 50-51 0.6879466429497185 1104 52-53 0.6729622063513787 1104 54-55 0.6602189538937182 1104 56-57 0.7457923291173216 1104 58-59 0.68898966915404 1104 60-61 0.9347411016506015 1104 62-63 1.045957053750982 1104 64-65 0.7633054625730153 1104 66-67 0.5077577442570345 1104 68-69 0.630396625405421 1104 70-71 0.7370166811112959 1104 72-73 0.8633752174358698 1104 74-75 0.7703338922503704 1104 76-77 0.7251674717751051 1104 78-79 0.9441956854152416 1104 80-81 0.9311550429072817 1104 82-83 1.2563512810151316 1104 84-85 1.3384138070507703 1104 86-87 1.4170630818360586 1104 88-89 1.3181044512180122 1104 90-91 1.232411283499662 1104 92-93 1.3161342454769454 1104 94-95 1.6217274667720112 1104 96-97 1.7749775875859868 1104 98-99 1.8499292169792056 1104 100-101 2.454674769498684 1105 1 0.3547465252649822 1105 2 0.2687783533533299 1105 3 0.3067623533812309 1105 4 0.2834025191647669 1105 5 0.3113566643303258 1105 6 0.33069940470042525 1105 7 0.31587007601441286 1105 8 0.28028951618217945 1105 9 0.3245874024446138 1105 10-11 0.44874817381519705 1105 12-13 0.37913404154148367 1105 14-15 0.4389184154581969 1105 16-17 0.43615839391103606 1105 18-19 0.5455061218632835 1105 20-21 0.4513108401273982 1105 22-23 0.5665237855874494 1105 24-25 0.6008584359431524 1105 26-27 0.512089063742792 1105 28-29 0.4447881649965524 1105 30-31 0.48798592106763294 1105 32-33 0.5669916045461392 1105 34-35 0.4870123519162419 1105 36-37 0.5045569005785637 1105 38-39 0.5897219238802194 1105 40-41 0.7769462944151044 1105 42-43 1.0226972604127766 1105 44-45 0.9521121092823392 1105 46-47 0.9665217125443988 1105 48-49 0.9743132994991086 1105 50-51 0.8614528494842162 1105 52-53 0.827125746413067 1105 54-55 0.888367888278438 1105 56-57 0.8279853664404442 1105 58-59 0.9158721455617922 1105 60-61 0.8554969122568892 1105 62-63 0.8822681835539612 1105 64-65 0.9234926978280456 1105 66-67 0.9914107638012055 1105 68-69 1.0446557249236683 1105 70-71 1.2178802729876104 1105 72-73 1.0273463359001305 1105 74-75 1.0460575163083625 1105 76-77 1.2956956297599724 1105 78-79 1.3383757602976338 1105 80-81 1.4093876515838453 1105 82-83 1.5115017630083223 1105 84-85 1.644859069427003 1105 86-87 1.8169038567331839 1105 88-89 1.6843520715016354 1105 90-91 2.018918401425278 1105 92-93 2.149566418559292 1105 94-95 2.1583911995529164 1105 96-97 2.3086941800760847 1105 98-99 2.5078371206137398 1105 100-101 2.6166956426972448 1106 1 0.1797384287583661 1106 2 0.18818876186387357 1106 3 0.2006190905438885 1106 4 0.10092504430137694 1106 5 0.200582814803127 1106 6 0.044542518872269454 1106 7 0.06906047402557647 1106 8 0.06168143069376697 1106 9 0.16703647067108562 1106 10-11 0.14072189486801534 1106 12-13 0.04224341565143419 1106 14-15 -0.026377456700700463 1106 16-17 0.03395924632408054 1106 18-19 0.1862567021240693 1106 20-21 0.27313420818322953 1106 22-23 0.23127372187175865 1106 24-25 0.1481574119189304 1106 26-27 0.1791418577796975 1106 28-29 0.28958742645608027 1106 30-31 0.41339844175684703 1106 32-33 0.4279949776671188 1106 34-35 0.45941442964964807 1106 36-37 0.3872396563165168 1106 38-39 0.5560079633633634 1106 40-41 0.7170735974399989 1106 42-43 0.7364672319071914 1106 44-45 0.7183140146290299 1106 46-47 0.7766207239281826 1106 48-49 0.9782429466732694 1106 50-51 0.88956103218419 1106 52-53 0.8093023334286542 1106 54-55 0.9591629130787496 1106 56-57 0.8952156551574575 1106 58-59 0.8929774177983063 1106 60-61 0.8797528693736183 1106 62-63 1.023505490223954 1106 64-65 1.0954233195247625 1106 66-67 1.052735082176973 1106 68-69 1.018413817084518 1106 70-71 1.1001033129577529 1106 72-73 1.0668025983173344 1106 74-75 1.3311756958874064 1106 76-77 1.521637104385885 1106 78-79 1.4467582320494508 1106 80-81 1.4688166787167027 1106 82-83 1.323134497445313 1106 84-85 1.3501343279201805 1106 86-87 1.5729148610289485 1106 88-89 1.685542010599324 1106 90-91 1.931660390697509 1106 92-93 1.911273459003315 1106 94-95 1.9767858207447198 1106 96-97 2.0162345883967525 1106 98-99 1.9857874782640508 1106 100-101 2.5823592339839863 1107 1 -0.013080376369750013 1107 2 0.12385483298497135 1107 3 0.22559883093656907 1107 4 0.14404786201651376 1107 5 0.09061329767948223 1107 6 0.04581233155712283 1107 7 0.14281623925950981 1107 8 0.11654086666840868 1107 9 0.2152480173045177 1107 10-11 0.27947887910106317 1107 12-13 0.314859930114352 1107 14-15 0.5551319854084298 1107 16-17 0.48385759285578445 1107 18-19 0.5664242184041512 1107 20-21 0.4510918870614731 1107 22-23 0.4878930450350083 1107 24-25 0.5050211141271888 1107 26-27 0.532680957110486 1107 28-29 0.5108173360079249 1107 30-31 0.5922418037932644 1107 32-33 0.5828461728918057 1107 34-35 0.6799050412502012 1107 36-37 0.7271516356288146 1107 38-39 0.8743497138460938 1107 40-41 0.7770324252841547 1107 42-43 0.7369366650436433 1107 44-45 0.9191431973098716 1107 46-47 0.9226183786054776 1107 48-49 1.015668457402306 1107 50-51 0.9187871283487397 1107 52-53 0.9290371392497221 1107 54-55 0.9690582634528013 1107 56-57 1.007051188744704 1107 58-59 1.171792529596054 1107 60-61 1.2697829573258517 1107 62-63 1.374061069474962 1107 64-65 1.3684259381349548 1107 66-67 1.3730962113229452 1107 68-69 1.434135825257865 1107 70-71 1.5144815259945652 1107 72-73 1.6584039313705397 1107 74-75 1.5273471435054624 1107 76-77 1.485935437437508 1107 78-79 1.4802821557627475 1107 80-81 1.5375596721046172 1107 82-83 1.4832096172784546 1107 84-85 1.5399353862115035 1107 86-87 1.5895212886032866 1107 88-89 1.637315456849361 1107 90-91 1.822685819071701 1107 92-93 1.7276357181588402 1107 94-95 2.055888595270954 1107 96-97 1.8885273177384043 1107 98-99 2.083055577504794 1107 100-101 2.6808315885057894 1108 1 0.18430431950437054 1108 2 0.19047885882349647 1108 3 0.27278045364689163 1108 4 0.26342101160114595 1108 5 0.23982595444500987 1108 6 0.3078477809037494 1108 7 0.26255380159526 1108 8 0.31809892019020936 1108 9 0.29400653412950106 1108 10-11 0.22464232048806565 1108 12-13 0.3646703145168786 1108 14-15 0.3914766382183359 1108 16-17 0.4199848076839032 1108 18-19 0.5869265938914197 1108 20-21 0.6005033831497073 1108 22-23 0.5546100641689407 1108 24-25 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90-91 -0.17363809104531214 2316 92-93 -0.17735815800031673 2316 94-95 0.015676266414153872 2316 96-97 0.3769235617826858 2316 98-99 0.37980893014399797 2316 100-101 0.6397206202247823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9665.0 3 2178.0 4 2099.0 5 2366.0 6 2533.0 7 2963.0 8 3658.0 9 4585.0 10 5649.0 11 6926.0 12 8715.0 13 10757.0 14 12240.0 15 14607.0 16 17131.0 17 21543.0 18 19344.0 19 15083.0 20 15124.0 21 16271.0 22 17591.0 23 19690.0 24 22015.0 25 24827.0 26 28909.0 27 33336.0 28 38790.0 29 45821.0 30 55408.0 31 67773.0 32 85141.0 33 107920.0 34 142953.0 35 195047.0 36 272336.0 37 348396.0 38 248532.0 39 27564.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56806253081427 18.912331851201927 14.78064302872715 22.738962589256655 2 14.890877800396993 19.522847381219506 41.6266543473736 23.95962047100989 3 19.500259129032045 24.0524531981497 29.41533282471964 27.03195484809861 4 12.437326901965799 16.34862559462997 37.51463577661352 33.699411726790714 5 13.585896546456553 37.400623524736716 34.2465099920778 14.766969936728936 6 31.92062924380661 36.20657905750397 17.51569431656483 14.357097382124588 7 28.691848712939994 32.58353957100198 20.32822858116769 18.39638313489034 8 26.070327703518515 35.97494282589241 19.266456706974328 18.688272763614744 9 25.94179363511518 17.305402557581605 20.468262580542714 36.284541226760496 10-11 24.931562098897587 27.083779354989268 26.92727014453638 21.05738840157676 12-13 25.71942906815321 24.194569953254327 27.812673355828956 22.27332762276351 14-15 22.468163314895996 25.60443855653572 26.323086393731153 25.604311734837133 16-17 21.844152668719794 29.117902339622407 26.84833518574789 22.189609805909903 18-19 21.76597125679792 27.472202755209995 29.442695583867057 21.319130404125026 20-21 22.382205093961264 26.50991196940651 29.78969891665384 21.318184019978386 22-23 22.060991734447377 26.494172219891997 29.586007241551226 21.858828804109397 24-25 21.85344393638811 26.595273918045848 29.76985817392365 21.78142397164239 26-27 21.706235397596252 26.990035618959308 29.60545738051011 21.69827160293433 28-29 21.5788043816021 27.07148348984872 29.689912589660977 21.6597995388882 30-31 21.718017983741074 26.803307587921214 29.695367094065872 21.783307334271836 32-33 21.439515275980277 26.876958613485556 29.63326080195789 22.05026530857627 34-35 21.922680263322764 26.974197839056973 29.461707154122546 21.641414743497716 36-37 21.913612651749734 26.76798109135543 29.409257019098845 21.909149237795987 38-39 21.68164338940823 27.048511597037777 29.48868649411798 21.78115851943601 40-41 21.969432813838083 26.894396911886986 29.387258997930456 21.748911276344476 42-43 21.732918843503374 26.853163418541815 29.34568213411707 22.068235603837742 44-45 21.844331113462946 27.15266941126136 29.09090309643134 21.912096378844353 46-47 21.939711677777865 27.356246571472266 28.624158290893348 22.07988345985652 48-49 22.01096488725037 27.360272511562844 28.539270554724883 22.089492046461903 50-51 22.111312097134846 27.570606593068746 28.378220352891546 21.93986095690486 52-53 22.204133222313047 27.605259966582285 28.261258943653147 21.929347867451522 54-55 22.090643538126862 27.577618389745755 28.310107821531282 22.0216302505961 56-57 22.04875505783901 27.82342658672505 28.23467155931294 21.893146796123002 58-59 22.173570189762366 27.50839275028652 28.248780388855643 22.06925667109547 60-61 22.039658275182756 27.55704690158156 28.471404978292902 21.93188984494278 62-63 22.0027961586167 27.61869789694687 28.542779375578675 21.83572656885775 64-65 21.91904374913629 27.586437729715612 28.44440612769367 22.05011239345443 66-67 21.864736264471933 27.654725654596835 28.319313624256242 22.16122445667499 68-69 21.91400610594027 27.62607037173506 28.377127905475845 22.082795616848824 70-71 21.914490218248773 27.64316228317188 28.267360513274735 22.174986985304614 72-73 22.00709265718528 27.622666580449167 28.18424679505467 22.18599396731089 74-75 21.920464288404126 27.84642534090411 28.100748311550582 22.132362059141187 76-77 22.064122467313197 27.67706393351525 28.10351225106834 22.15530134810321 78-79 22.049225089237183 27.7367908297682 28.00904889002971 22.204935190964907 80-81 21.8789777821174 27.958113058728202 28.00635050260018 22.156558656554218 82-83 21.963570222665304 27.940299658161756 28.017016083566887 22.07911403560606 84-85 22.03569407987081 27.8433045261343 27.913498775024138 22.207502618970757 86-87 22.003562529131944 27.824093023397356 28.022152195667243 22.150192251803453 88-89 22.074707801742917 27.786997957414606 27.901478097367466 22.236816143475014 90-91 22.017376519836816 27.813895027722392 27.980645302004405 22.18808315043639 92-93 22.076068476642227 27.596683289443252 28.083498005672574 22.24375022824195 94-95 21.93372714378207 27.656692575515766 28.011583535433175 22.397996745268987 96-97 22.060033598529273 27.553304383657167 27.91586421122698 22.470797806586578 98-99 21.95004555813855 27.540852070327666 27.867125942069528 22.64197642946426 100-101 22.229358733339993 27.055860371638396 28.357725663496907 22.357055231524704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2651.0 1 1536.5 2 436.0 3 440.5 4 366.0 5 322.5 6 536.5 7 1213.5 8 2034.5 9 2421.5 10 2238.5 11 1977.0 12 2015.0 13 2174.5 14 2336.0 15 2511.5 16 2673.0 17 2792.0 18 3013.0 19 3401.5 20 3835.0 21 4524.0 22 5549.5 23 6693.5 24 8133.5 25 10120.5 26 12802.0 27 16033.5 28 18776.0 29 21692.0 30 25281.0 31 28853.0 32 32832.0 33 36376.5 34 40171.5 35 44049.0 36 48228.5 37 52471.0 38 55455.0 39 57847.5 40 60736.0 41 64174.0 42 66918.0 43 69618.5 44 72640.5 45 74958.5 46 75651.5 47 76556.0 48 78522.5 49 77306.0 50 73470.5 51 70100.0 52 66227.0 53 63603.0 54 61697.5 55 57285.0 56 51624.0 57 45896.5 58 40920.0 59 35945.5 60 29599.0 61 23598.5 62 18619.0 63 14006.0 64 10362.0 65 8023.5 66 5937.5 67 4306.0 68 3158.5 69 2253.0 70 1665.0 71 1186.5 72 871.0 73 681.0 74 527.0 75 399.0 76 285.0 77 208.0 78 137.0 79 81.5 80 59.0 81 47.0 82 33.5 83 22.5 84 16.0 85 13.0 86 10.0 87 8.0 88 4.0 89 4.0 90 7.5 91 6.0 92 3.5 93 3.5 94 2.5 95 2.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3051396388438508 2 0.2874731269068064 3 0.27476741201798643 4 0.2604924654098302 5 0.2570502797028989 6 0.2143266806345163 7 0.20541749409892945 8 0.21852817201209424 9 0.21918623692665462 10-11 0.2206542278899047 12-13 0.2171867319939519 14-15 0.21349144439680506 16-17 0.2119981432445334 18-19 0.21288399986028775 20-21 0.20038076648364023 22-23 0.19296488110032503 24-25 0.19304081166738968 26-27 0.2058730775013174 28-29 0.22189442715196084 30-31 0.2158452919758095 32-33 0.22212221885315486 34-35 0.2136179953419128 36-37 0.19762195588029088 38-39 0.19891277552039013 40-41 0.1923321263747862 42-43 0.20599962844642516 44-45 0.2013425536664593 46-47 0.2014437944225455 48-49 0.21242841645789978 50-51 0.2213882233715298 52-53 0.23680212848565593 54-55 0.2090368511290116 56-57 0.17970234205303093 58-59 0.1796517216749878 60-61 0.18570085685113916 62-63 0.1951668675452002 64-65 0.18332169908311308 66-67 0.1893708342592644 68-69 0.19248398750891552 70-71 0.18625768100961332 72-73 0.1888899406678549 74-75 0.19152220032609646 76-77 0.2070120360072873 78-79 0.2124790368359429 80-81 0.2020512389590628 82-83 0.19957084043495055 84-85 0.19324329317956213 86-87 0.19519217773422176 88-89 0.2008616600750498 90-91 0.20136786385548086 92-93 0.19726761323398917 94-95 0.19729292342301072 96-97 0.186105819875484 98-99 0.19400259885020874 100-101 0.18354949078430707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1975489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.36198529731354 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4581973894189 50.346291347393965 2 12.418104268491275 15.736714801613013 3 3.6547683915374094 6.947201432690388 4 1.5548261833049912 3.9406749506579595 5 0.8194339008938668 2.5960479390278737 6 0.4966144459540771 1.8879886333785443 7 0.3104367519791549 1.3768922240254284 8 0.22187716930851525 1.1246862351628553 9 0.1688616731582383 0.9629469766738854 >10 0.8298341783511733 9.335225904753463 >50 0.04594257726623521 1.9731623945690402 >100 0.019904630122226777 2.327670481107728 >500 7.190641283770938E-4 0.2550928283874959 >1k 3.195840570564728E-4 0.4861900225904777 >5k 1.597920285282364E-4 0.703213827967964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7876 0.39868609746751316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6015 0.3044815739292904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.21311179156148174 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2821 0.14280008645960568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 5.06203780431073E-5 0.0 0.0 5.06203780431073E-5 0.0 24-25 5.06203780431073E-5 0.0 0.0 1.518611341293219E-4 0.0 26-27 5.06203780431073E-5 0.0 0.0 2.2779170119398287E-4 0.0 28-29 5.06203780431073E-5 0.0 0.0 5.568241584741803E-4 0.0 30-31 5.06203780431073E-5 0.0 0.0 0.0020501253107458456 0.0 32-33 5.06203780431073E-5 0.0 0.0 0.004252111755621013 0.0 34-35 5.06203780431073E-5 0.0 0.0 0.006200996310280644 0.0 36-37 5.06203780431073E-5 0.0 0.0 0.008706705023414456 0.0 38-39 5.06203780431073E-5 0.0 0.0 0.013237228858272559 0.0 40-41 5.06203780431073E-5 0.0 0.0 0.01753996099193668 0.0 42-43 1.012407560862146E-4 0.0 0.0 0.02212110520483789 0.0 44-45 1.518611341293219E-4 0.0 0.0 0.024449642594820825 0.0 46-47 2.5310189021553653E-4 0.0 0.0 0.02753748565545037 0.0 48-49 2.5310189021553653E-4 0.0 0.0 0.032498282703674886 0.0 50-51 2.5310189021553653E-4 0.0 0.0 0.03831962617863223 0.0 52-53 2.5310189021553653E-4 0.0 0.0 0.049177697268878745 0.0 54-55 2.7841207923709016E-4 0.0 0.0 0.05811219399348718 0.0 56-57 3.543426463017511E-4 0.0 0.0 0.07658863197922135 0.0 58-59 3.543426463017511E-4 0.0 0.0 0.10448046028097346 0.0 60-61 3.543426463017511E-4 0.0 0.0 0.13135988102186344 0.0 62-63 3.543426463017511E-4 0.0 0.0 0.14710281859326982 0.0 64-65 3.543426463017511E-4 0.0 0.0 0.17400754952318137 0.0 66-67 4.049630243448584E-4 0.0 0.0 0.22300807546890922 0.0 68-69 4.049630243448584E-4 0.0 0.0 0.29099124318080233 0.0 70-71 4.049630243448584E-4 0.0 0.0 0.3548741602712037 0.0 72-73 4.5558340238796574E-4 0.0 0.0 0.4271347499277394 5.06203780431073E-5 74-75 5.062037804310731E-4 0.0 0.0 0.5120251239060303 5.06203780431073E-5 76-77 5.062037804310731E-4 0.0 0.0 0.5794261572704278 5.06203780431073E-5 78-79 5.062037804310731E-4 0.0 0.0 0.6669994112850034 5.06203780431073E-5 80-81 5.568241584741803E-4 0.0 0.0 0.7808446415039516 5.06203780431073E-5 82-83 5.568241584741803E-4 0.0 0.0 0.8846164164923216 5.06203780431073E-5 84-85 5.568241584741803E-4 0.0 0.0 1.0059028422836067 5.06203780431073E-5 86-87 5.568241584741803E-4 0.0 0.0 1.1615858149551834 5.06203780431073E-5 88-89 5.568241584741803E-4 0.0 0.0 1.363586433536203 5.06203780431073E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4850 0.0 44.00979 1 GTATCAA 7890 0.0 36.27139 1 TATCAAC 9650 0.0 29.451563 2 ATCAACG 9670 0.0 29.33852 3 TCAACGC 9775 0.0 29.024849 4 CAACGCA 10040 0.0 28.306087 5 AACGCAG 10265 0.0 27.81465 6 ACGCAGA 11760 0.0 24.197893 7 TACATGG 7995 0.0 24.07526 2 CGCAGAG 11780 0.0 23.95517 8 GTACATG 8100 0.0 23.945274 1 TAACGCA 245 2.9649527E-10 23.277029 4 ACATGGG 8015 0.0 22.88622 3 GAGTACT 6825 0.0 22.06219 12-13 GCAGAGT 12645 0.0 21.564535 9 GTACTTT 7415 0.0 20.24216 14-15 AGTACTT 7330 0.0 19.667389 12-13 AGAGTAC 11470 0.0 19.444027 10-11 CAGAGTA 12210 0.0 19.41328 10-11 CATGGGA 4535 0.0 19.177244 4 >>END_MODULE