FastQCFastQC Report
Thu 26 May 2016
SRR937581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975489
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97750.4948141953713739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.42435062913536853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.2923833035769878No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.11313654492634481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54700.045.5187641
GTATCAA93200.038.4416431
ATCAACG118750.029.8821983
TATCAAC121250.029.3444252
TCAACGC121200.029.2781454
CAACGCA123250.028.752625
AACGCAG125600.028.2867166
ACGCAGA142000.024.988887
CGCAGAG143500.024.6614698
GTACATG77850.024.6586481
ACATGGG74800.024.0057753
TACATGG77950.023.7060412
GAGTACT95950.022.6743612-13
GCAGAGT156200.022.321829
AGTACTT100350.021.3961512-13
GTACTTT102150.021.20298814-15
TGGTATC29750.020.7578282
AGAGTAC141800.020.65244110-11
CATGGGA41500.020.3749924
CATGGGG35050.019.6519434
Produced by FastQC (version 0.11.5)