##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937581_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975489 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.297816894956135 33.0 31.0 34.0 31.0 34.0 2 32.51338023142625 34.0 31.0 34.0 31.0 34.0 3 32.57964180008089 34.0 31.0 34.0 31.0 34.0 4 36.16034359087801 37.0 35.0 37.0 35.0 37.0 5 36.08998632743589 37.0 35.0 37.0 35.0 37.0 6 36.136034672934144 37.0 35.0 37.0 35.0 37.0 7 36.09570744256232 37.0 35.0 37.0 35.0 37.0 8 36.061608543504924 37.0 35.0 37.0 35.0 37.0 9 37.771464685452564 39.0 37.0 39.0 35.0 39.0 10-11 37.696700158796126 39.0 37.0 39.0 35.0 39.0 12-13 37.595229839295484 39.0 37.0 39.0 35.0 39.0 14-15 38.84789107912016 40.0 38.0 41.0 35.0 41.0 16-17 38.80894553196703 40.0 38.0 41.0 35.0 41.0 18-19 38.782046622380584 40.0 38.0 41.0 35.0 41.0 20-21 38.682114403066784 40.0 38.0 41.0 34.0 41.0 22-23 38.6361627424906 40.0 38.0 41.0 34.0 41.0 24-25 38.66598877543737 40.0 38.0 41.0 34.0 41.0 26-27 38.501807400598025 40.0 38.0 41.0 34.0 41.0 28-29 38.46846679480372 40.0 38.0 41.0 34.0 41.0 30-31 38.47268094127581 40.0 38.0 41.0 34.0 41.0 32-33 38.345942953871166 40.0 38.0 41.0 34.0 41.0 34-35 38.206345365628465 40.0 38.0 41.0 34.0 41.0 36-37 38.04092682874975 40.0 37.5 41.0 33.5 41.0 38-39 37.90258918171652 40.0 37.0 41.0 33.0 41.0 40-41 37.72970920111426 40.0 37.0 41.0 33.0 41.0 42-43 37.51028656702214 40.0 37.0 41.0 33.0 41.0 44-45 37.21292550857028 40.0 37.0 41.0 32.0 41.0 46-47 36.87923040826854 40.0 36.5 41.0 31.0 41.0 48-49 36.54795723995426 39.5 36.0 41.0 30.5 41.0 50-51 36.30711509909698 39.0 36.0 41.0 30.0 41.0 52-53 36.04467552084573 39.0 35.0 40.5 29.5 41.0 54-55 35.828352625603074 39.0 35.0 40.0 29.0 41.0 56-57 35.56322561148151 38.5 35.0 40.0 28.0 41.0 58-59 35.30583035390225 38.0 35.0 40.0 28.0 41.0 60-61 34.97069636935463 38.0 34.0 40.0 27.5 41.0 62-63 34.568364339158556 37.5 34.0 40.0 26.0 41.0 64-65 34.17677850901726 37.0 34.0 39.5 26.0 41.0 66-67 33.75930820166551 36.5 33.0 39.0 25.5 40.5 68-69 33.295624526383094 36.0 33.0 39.0 24.5 40.0 70-71 32.87000231335128 35.5 32.0 38.5 23.5 40.0 72-73 32.39962004344241 35.0 32.0 38.0 22.5 40.0 74-75 31.921273163252238 35.0 31.0 37.0 21.5 39.0 76-77 30.659281069142878 33.5 29.5 35.5 20.0 38.5 78-79 31.259573958650236 34.0 31.0 36.0 20.0 38.5 80-81 31.08099893241623 34.0 31.0 36.0 20.0 37.5 82-83 30.69173860244223 34.0 31.0 35.0 18.5 37.0 84-85 30.280027628602333 34.0 30.0 35.0 17.0 36.5 86-87 29.92597098743653 34.0 30.0 35.0 11.5 36.0 88-89 29.505831973754347 34.0 29.5 35.0 7.0 36.0 90-91 28.983393731881065 34.0 29.0 35.0 2.0 35.0 92-93 28.51017039325453 34.0 29.0 35.0 2.0 35.0 94-95 27.99976259042698 33.0 28.0 35.0 2.0 35.0 96-97 27.46122732143788 33.0 27.0 35.0 2.0 35.0 98-99 26.60344476734621 33.0 25.5 34.5 2.0 35.0 100-101 24.081629662326645 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22689057037341342 1101 2 0.17603890053269566 1101 3 0.17066527898052186 1101 4 0.13878058545964933 1101 5 0.13505064261016742 1101 6 0.09514917719616989 1101 7 0.15323488043684108 1101 8 0.15750092653850345 1101 9 0.2689736613358207 1101 10-11 0.21850388965794565 1101 12-13 0.24022031405184663 1101 14-15 0.31476720844973727 1101 16-17 0.34758691930699115 1101 18-19 0.31318693730176506 1101 20-21 0.3464168261124101 1101 22-23 0.25090238388607133 1101 24-25 0.25714864285205863 1101 26-27 0.14559168155347635 1101 28-29 0.32912317240363365 1101 30-31 0.3939429869553379 1101 32-33 0.375968590125872 1101 34-35 0.4256387654184408 1101 36-37 0.34613009234885084 1101 38-39 0.37937584851005113 1101 40-41 0.3581546137970406 1101 42-43 0.46309869285713745 1101 44-45 0.5199832759184346 1101 46-47 0.5589250830510437 1101 48-49 0.5520874748431197 1101 50-51 0.5385972804626107 1101 52-53 0.5833024084381506 1101 54-55 0.7016234673932971 1101 56-57 0.7140158610126477 1101 58-59 0.7029716599042217 1101 60-61 0.7759467645216986 1101 62-63 0.6123392378992563 1101 64-65 0.8072296557016401 1101 66-67 0.8686555079704021 1101 68-69 0.8478293805716035 1101 70-71 0.7221492595178418 1101 72-73 0.5740992898506576 1101 74-75 0.6552047563321857 1101 76-77 0.642103782312784 1101 78-79 0.7097014191415454 1101 80-81 0.8658959025089601 1101 82-83 0.7781201625853136 1101 84-85 0.8101871342646447 1101 86-87 0.8185682699968027 1101 88-89 0.9893569847994534 1101 90-91 0.9777477183543724 1101 92-93 0.9543914113843357 1101 94-95 1.030545478382063 1101 96-97 0.9789993950728473 1101 98-99 1.1926512834938663 1101 100-101 1.6955655815038781 1102 1 0.11708771925946593 1102 2 0.10706500711822997 1102 3 0.04481939125847134 1102 4 0.02376173342968002 1102 5 -0.01867641598242642 1102 6 -0.009674856892878836 1102 7 0.025377427955405096 1102 8 -0.01844821796373708 1102 9 0.053019773155760674 1102 10-11 0.06216474122989979 1102 12-13 0.060077104054137465 1102 14-15 0.055962267663922205 1102 16-17 0.14039666920066907 1102 18-19 0.08611382741201368 1102 20-21 0.11343630381712444 1102 22-23 0.08517934106210845 1102 24-25 0.033831319431378404 1102 26-27 0.08873870285095364 1102 28-29 0.16856368423011503 1102 30-31 0.1267069767155391 1102 32-33 0.21648154118420138 1102 34-35 0.21741676538572818 1102 36-37 0.29541394012586153 1102 38-39 0.15393951193793498 1102 40-41 0.1613548583418094 1102 42-43 0.024986921993139788 1102 44-45 0.185223056564233 1102 46-47 0.19400969227013576 1102 48-49 0.2660166100215804 1102 50-51 0.38689793967024855 1102 52-53 0.386132053153041 1102 54-55 0.393397377983149 1102 56-57 0.4776791866579586 1102 58-59 0.44452934466229266 1102 60-61 0.5413458264185138 1102 62-63 0.558706643925369 1102 64-65 0.5067754281032464 1102 66-67 0.35471410465964937 1102 68-69 0.40935856236897195 1102 70-71 0.407572980360591 1102 72-73 0.5207198283398782 1102 74-75 0.616217960931916 1102 76-77 0.3107664741045859 1102 78-79 0.1948025903392434 1102 80-81 0.1330848849082784 1102 82-83 0.2872650328702626 1102 84-85 0.43993725285659124 1102 86-87 0.3501389794219989 1102 88-89 0.3971992861375 1102 90-91 0.4353780877069404 1102 92-93 0.5631006607382147 1102 94-95 0.7197995145975895 1102 96-97 0.577239020322299 1102 98-99 0.6974166350167508 1102 100-101 1.1581555478074108 1103 1 0.14621994012087214 1103 2 0.17375718919620198 1103 3 0.14217727041747708 1103 4 0.10204429109450786 1103 5 0.0425962927336343 1103 6 0.09130110758966481 1103 7 0.036113037263426406 1103 8 -0.021716742152349866 1103 9 0.19062183480099293 1103 10-11 0.19645617825786843 1103 12-13 0.15888043126376772 1103 14-15 0.1487369410666588 1103 16-17 0.06117067920271069 1103 18-19 0.14373145345815175 1103 20-21 0.21588131810575106 1103 22-23 0.1675086429604562 1103 24-25 0.11344004059895951 1103 26-27 0.0896113299176946 1103 28-29 0.054287173717774806 1103 30-31 0.1618601967604434 1103 32-33 0.23978285267634902 1103 34-35 0.2625197200703582 1103 36-37 0.2907707731530067 1103 38-39 0.2859894200824513 1103 40-41 0.23475567993805413 1103 42-43 0.3895026394865013 1103 44-45 0.3811973625450449 1103 46-47 0.3080316865546848 1103 48-49 0.41186078740472 1103 50-51 0.48642207724935105 1103 52-53 0.4034276747084604 1103 54-55 0.3564274606530802 1103 56-57 0.3648430211139626 1103 58-59 0.380104206879075 1103 60-61 0.6093871358694258 1103 62-63 0.531167860500176 1103 64-65 0.5953081643759575 1103 66-67 0.5133555365845268 1103 68-69 0.5937916099297027 1103 70-71 0.4724057268394617 1103 72-73 0.5272007428122194 1103 74-75 0.6209631895500074 1103 76-77 0.5806761495485553 1103 78-79 0.6949990590063173 1103 80-81 0.8547978620464711 1103 82-83 0.8420912729192516 1103 84-85 0.7848016384454866 1103 86-87 0.8287755634187342 1103 88-89 0.9312311038553993 1103 90-91 0.7790936419016745 1103 92-93 0.9869569324263168 1103 94-95 1.1537981622807898 1103 96-97 1.284137749654814 1103 98-99 1.4190001214425543 1103 100-101 1.925906619454686 1104 1 0.17772309854679236 1104 2 0.1477580722938967 1104 3 0.14189702206878252 1104 4 0.10670127973138932 1104 5 0.08293628534445219 1104 6 0.09322004282616803 1104 7 0.041738279439449855 1104 8 0.13262170053190658 1104 9 0.1174634989895793 1104 10-11 0.13763015751408147 1104 12-13 0.17327740169802297 1104 14-15 0.1817598385282082 1104 16-17 0.22098522868771653 1104 18-19 0.25903675294541273 1104 20-21 0.19045643998224904 1104 22-23 0.13134804533761013 1104 24-25 0.07339561537840922 1104 26-27 0.18020160863414247 1104 28-29 0.22290499066423308 1104 30-31 0.22610082285868316 1104 32-33 0.20481353581548234 1104 34-35 0.13843030192360573 1104 36-37 0.10387305929469193 1104 38-39 0.07419285695448252 1104 40-41 0.21694706883445036 1104 42-43 0.07727106063398992 1104 44-45 0.1391069441549817 1104 46-47 0.2557960076057526 1104 48-49 0.31799606355297527 1104 50-51 0.3715263368440276 1104 52-53 0.45212397921496006 1104 54-55 0.43522095096153635 1104 56-57 0.25971024106836893 1104 58-59 0.3024765406900016 1104 60-61 0.1948971247856477 1104 62-63 0.16761614071247521 1104 64-65 0.2793790645048162 1104 66-67 0.2318634168797331 1104 68-69 0.24203645326996792 1104 70-71 0.34032449987434177 1104 72-73 0.26038922109072615 1104 74-75 0.422626431960321 1104 76-77 0.34157226842431854 1104 78-79 0.3846427110658297 1104 80-81 0.3497099127254657 1104 82-83 0.35188870112250825 1104 84-85 0.2912991648708321 1104 86-87 0.38237170842697665 1104 88-89 0.44199408223753167 1104 90-91 0.37339270563790095 1104 92-93 0.4918491134072731 1104 94-95 0.45064086275748494 1104 96-97 0.7582093706797473 1104 98-99 1.0048852015971832 1104 100-101 1.3025180474596922 1105 1 0.16179619718877802 1105 2 0.2211401822911938 1105 3 0.14871781856997757 1105 4 0.13634881336454896 1105 5 0.11504355041972758 1105 6 0.1779464839999676 1105 7 0.1699612033656166 1105 8 0.14290753971918946 1105 9 0.19381250909587067 1105 10-11 0.23721866539897718 1105 12-13 0.19903798393448113 1105 14-15 0.22554905932783242 1105 16-17 0.2729942899060731 1105 18-19 0.3747308543713359 1105 20-21 0.3384346690536759 1105 22-23 0.2697282476586338 1105 24-25 0.2524646986301846 1105 26-27 0.30996226112772973 1105 28-29 0.21413248520710937 1105 30-31 0.193154009458695 1105 32-33 0.27933439681662975 1105 34-35 0.2822343303894428 1105 36-37 0.1530620773244209 1105 38-39 0.22229610956692625 1105 40-41 0.14896024302237265 1105 42-43 0.1792003751293052 1105 44-45 0.19578834855600746 1105 46-47 0.2620386464636084 1105 48-49 0.3320040670111837 1105 50-51 0.15148624636844943 1105 52-53 0.15165654973800002 1105 54-55 0.25030710864781724 1105 56-57 0.06588008573179849 1105 58-59 0.278476289911076 1105 60-61 0.18325883582001978 1105 62-63 0.30335889520186043 1105 64-65 0.24277851435244457 1105 66-67 0.27049373946730526 1105 68-69 0.2717450959704948 1105 70-71 0.26598416066901365 1105 72-73 0.2358583826011298 1105 74-75 0.2946451388973337 1105 76-77 0.20111252820005987 1105 78-79 0.2900006223603455 1105 80-81 0.3710460033259402 1105 82-83 0.36153832295374144 1105 84-85 0.4992051413132259 1105 86-87 0.5735334344885707 1105 88-89 0.7550203398645756 1105 90-91 0.7040581382510673 1105 92-93 0.6496449227645478 1105 94-95 0.9109276533434176 1105 96-97 1.1561325893931276 1105 98-99 1.272390570900864 1105 100-101 1.2791856053510813 1106 1 -0.12530258447083042 1106 2 -0.003297387959875664 1106 3 -0.03490052160204016 1106 4 -0.03458308318094794 1106 5 0.060066465338614705 1106 6 0.022273192951210774 1106 7 0.04741793485871426 1106 8 0.03360724102428492 1106 9 -0.1766881799429143 1106 10-11 -0.2782127012298261 1106 12-13 -0.17080014209396666 1106 14-15 -0.04846801407185808 1106 16-17 0.11898643221631744 1106 18-19 -0.009839437078831281 1106 20-21 0.048964968173798695 1106 22-23 0.02517349936170632 1106 24-25 0.01005462200286189 1106 26-27 0.12373458602456822 1106 28-29 0.12398152781359073 1106 30-31 0.07809924070321728 1106 32-33 0.046496517093146394 1106 34-35 -0.16144406968200542 1106 36-37 0.10703661297539213 1106 38-39 0.11866965015656206 1106 40-41 6.636651101743496E-4 1106 42-43 -0.0827905444814121 1106 44-45 -0.05285637245481922 1106 46-47 0.07003056038684718 1106 48-49 0.22512697051072195 1106 50-51 0.0724536445242947 1106 52-53 0.02073583720262917 1106 54-55 0.04495055079837584 1106 56-57 0.04271110635696829 1106 58-59 0.09733864983348184 1106 60-61 0.15934305232555346 1106 62-63 0.16752665856297 1106 64-65 -0.08365645271462796 1106 66-67 -0.09547932423742367 1106 68-69 0.08371425998044657 1106 70-71 0.26720004067971814 1106 72-73 0.11132622161877492 1106 74-75 0.10376468024059804 1106 76-77 0.13529294377348933 1106 78-79 0.1950822867614157 1106 80-81 0.003179119254809848 1106 82-83 0.09909771627868835 1106 84-85 0.26437021243545544 1106 86-87 0.45540239436760643 1106 88-89 0.17200233125486974 1106 90-91 0.11933685464120103 1106 92-93 0.23153329821353452 1106 94-95 0.5154101628833878 1106 96-97 0.26679612318196533 1106 98-99 0.010181560418331514 1106 100-101 0.5930386947399953 1107 1 0.11288378706640856 1107 2 0.1434437771926511 1107 3 0.13367863093292698 1107 4 0.1303669799805789 1107 5 0.05031104974416678 1107 6 0.08263892427959263 1107 7 -0.002214022190393905 1107 8 0.06072062479206153 1107 9 0.08398280140139747 1107 10-11 0.058542912879808284 1107 12-13 0.07508395873490059 1107 14-15 0.09821035814570678 1107 16-17 0.1252354481970528 1107 18-19 0.24126490240004017 1107 20-21 0.07737238663907675 1107 22-23 0.049745809704667465 1107 24-25 0.04637579325301999 1107 26-27 0.06127101777456545 1107 28-29 -0.009453327346676588 1107 30-31 -3.365069855476577E-4 1107 32-33 0.20951737593199482 1107 34-35 0.2247481456790581 1107 36-37 0.24607043901605863 1107 38-39 0.3567700598771779 1107 40-41 0.2958174459294014 1107 42-43 0.24694529340802518 1107 44-45 0.24437391938356967 1107 46-47 0.2770288527349507 1107 48-49 0.3771732829989105 1107 50-51 0.2675680119164099 1107 52-53 0.39140226902107855 1107 54-55 0.3830752351881799 1107 56-57 0.48740290494145455 1107 58-59 0.49508397537997695 1107 60-61 0.4606489610749165 1107 62-63 0.3523073098086371 1107 64-65 0.46589283355217503 1107 66-67 0.6195257913463337 1107 68-69 0.6701764454694654 1107 70-71 0.6037222546348602 1107 72-73 0.5479644642801631 1107 74-75 0.6944599257227928 1107 76-77 0.4689926350481386 1107 78-79 0.6487429437361172 1107 80-81 0.5423480883290921 1107 82-83 0.4137298128676541 1107 84-85 0.6310760819030286 1107 86-87 0.7024280891839361 1107 88-89 0.8021647135214032 1107 90-91 0.816278009367732 1107 92-93 0.8014538236837687 1107 94-95 1.1059009693131756 1107 96-97 1.016439769985002 1107 98-99 0.8799279506802371 1107 100-101 1.3559049276220065 1108 1 0.10066159508590289 1108 2 0.06674472164163348 1108 3 0.10767377411235657 1108 4 0.10236768158853238 1108 5 -0.018331611043507223 1108 6 0.07785770801801561 1108 7 0.007369579538227811 1108 8 0.08963171110495693 1108 9 0.11070892329368576 1108 10-11 0.11488021695125639 1108 12-13 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-0.015143636844086927 2316 58-59 0.02151617452322796 2316 60-61 0.006505757228971731 2316 62-63 0.06725947957649936 2316 64-65 -0.10310363021242352 2316 66-67 0.007551748337803588 2316 68-69 0.05693240475812189 2316 70-71 0.1464317298651423 2316 72-73 0.21229645382024387 2316 74-75 0.2816990673614619 2316 76-77 0.38272295276180657 2316 78-79 0.29251989547977786 2316 80-81 0.23561686245399116 2316 82-83 0.18301812615120738 2316 84-85 0.24281356254657638 2316 86-87 0.36170835637145515 2316 88-89 0.6458392441757219 2316 90-91 0.5018437772759512 2316 92-93 0.5817909384441897 2316 94-95 0.6624685356904898 2316 96-97 0.7589533894308467 2316 98-99 0.657291223584533 2316 100-101 1.3228781434061645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 9.0 5 37.0 6 73.0 7 149.0 8 405.0 9 825.0 10 1400.0 11 2490.0 12 3619.0 13 5435.0 14 7228.0 15 10159.0 16 13775.0 17 19099.0 18 17737.0 19 11039.0 20 9086.0 21 9129.0 22 9641.0 23 10443.0 24 12152.0 25 14521.0 26 17826.0 27 22011.0 28 27511.0 29 34705.0 30 44038.0 31 55727.0 32 71973.0 33 94534.0 34 131529.0 35 191700.0 36 295451.0 37 448271.0 38 351284.0 39 30469.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.689489004077515 19.478541898343675 15.42270039681334 22.409268700765473 2 15.466854029559263 20.06713274536077 40.834649041325974 23.631364183754 3 19.73430375972734 24.039566912293616 29.911783867184276 26.31434546079477 4 12.958468996015164 16.922249084783104 37.0837484206434 33.03553349855833 5 13.585055623758116 37.412729550833404 34.37221723007606 14.629997595332414 6 32.08947253059875 36.48377692814285 17.05856119674673 14.368189344511661 7 28.58836470362528 32.925619935114796 20.18102859595776 18.30498676530216 8 26.36258668106985 35.93241977049733 19.05938225927859 18.64561128915423 9 25.939450941007518 17.168812380124617 20.417324520662987 36.47441215820488 10-11 24.97260172038417 27.176739531326167 26.7892658475952 21.06139290069446 12-13 25.86367407810895 24.153866948403056 27.88742255244463 22.09503642104336 14-15 22.479193759114832 25.614619975104898 26.46458674282671 25.441599522953556 16-17 21.778683657565292 29.06434811836462 27.152391129487434 22.004577094582658 18-19 21.689946134855724 27.134471515660174 30.004646950704355 21.170935398779744 20-21 22.19087124746929 26.211481897975865 30.522198742738173 21.075448111816677 22-23 21.998334063540973 26.178032468802925 30.271371481810238 21.552261985845867 24-25 21.75256354249505 26.25370224789913 30.556965895532702 21.436768314073124 26-27 21.61541508528994 26.631417626460575 30.348183404687752 21.40498388356174 28-29 21.445323336039866 26.777249745376793 30.368980919964283 21.40844599861906 30-31 21.662788302035597 26.440086479853846 30.38019953540617 21.516925682704382 32-33 21.392450173096382 26.498046812713206 30.34625857192827 21.763244442262145 34-35 21.848767570965975 26.542693986147224 30.19708031783523 21.41145812505157 36-37 21.81170929127441 26.456928876869323 30.103303876394754 21.628057955461514 38-39 21.70763795698179 26.63393721756993 30.186728450525415 21.471696374922868 40-41 21.91667965926325 26.589939478122833 30.020718973173153 21.472661889440765 42-43 21.714237252521777 26.56257634310061 29.96105156964579 21.76213483473182 44-45 21.829101554096226 26.832470340254993 29.726209561278242 21.61221854437053 46-47 21.949755225161972 27.10539010847441 29.19082313290532 21.7540315334583 48-49 22.059095241735086 27.128979204642494 29.042252323348798 21.769673230273618 50-51 22.13707593411049 27.351784798599233 28.79621197587028 21.714927291419997 52-53 22.305110279024586 27.373171908322448 28.59570465844153 21.726013154211437 54-55 22.104552341217794 27.47993028561536 28.624760755438274 21.790756617728572 56-57 22.110373684692753 27.598432590614273 28.60175379361768 21.689439931075295 58-59 22.21308243174222 27.3448244966183 28.61119449412272 21.83089857751676 60-61 22.13279851216585 27.320222992889352 28.871079514996033 21.675898979948762 62-63 22.046060494388982 27.38577638245518 28.893225930389892 21.67493719276594 64-65 22.01098057240511 27.36051681381167 28.779355389981927 21.849147223801296 66-67 21.928722458085062 27.433157056303525 28.679658555426023 21.95846193018539 68-69 22.029760732659103 27.311870630512242 28.79557922114474 21.862789415683913 70-71 22.02414187069632 27.452974934307406 28.60972650315947 21.913156691836804 72-73 22.112727532271755 27.390585318369276 28.51681279926135 21.97987435009762 74-75 22.07028234528261 27.58782762141424 28.35606778878546 21.985822244517685 76-77 22.106374674827347 27.358365447744838 28.550525971038056 21.98473390638976 78-79 22.084962254915112 27.458036972111717 28.42789810522863 22.029102667744542 80-81 22.001970145113436 27.69248525301837 28.398158633128308 21.90738596873989 82-83 21.98814468345748 27.728178143595116 28.374146095768886 21.909531077178514 84-85 22.078625834795844 27.658020848572225 28.289334810777923 21.974018505854012 86-87 22.036796965207095 27.573982948019456 28.392362599842368 21.996857486931084 88-89 22.087088310792925 27.525615176799263 28.348829074725295 22.03846743768252 90-91 22.000930505231942 27.582316553019815 28.376916382612816 22.03983655913543 92-93 21.99317368133975 27.358326045936543 28.49255293434256 22.155947338381146 94-95 21.908448996678796 27.35831482736679 28.508966640664667 22.224269535289743 96-97 21.98017807236588 27.300455735263522 28.3906415069889 22.328724685381694 98-99 21.856974290260023 27.25278502356646 28.281169560566894 22.609071125606626 100-101 21.798354362609782 27.383623870222216 28.548733117408293 22.269288649759712 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 331.0 1 249.0 2 247.0 3 383.5 4 331.5 5 240.0 6 535.5 7 1403.0 8 2368.0 9 2828.0 10 2601.5 11 2251.5 12 2318.5 13 2554.0 14 2746.5 15 2874.5 16 3052.0 17 3232.0 18 3466.5 19 3822.0 20 4242.0 21 4887.0 22 5825.0 23 6969.0 24 8399.5 25 10464.0 26 13094.0 27 16154.0 28 18754.5 29 21576.0 30 25445.5 31 29038.5 32 32523.5 33 36099.5 34 40018.0 35 43901.5 36 48339.0 37 52657.5 38 55671.0 39 58050.5 40 61028.0 41 64533.5 42 66987.5 43 69973.0 44 73344.5 45 75159.5 46 75789.0 47 77278.0 48 79428.5 49 77518.5 50 73644.0 51 70400.0 52 66189.5 53 63339.0 54 61689.0 55 57197.5 56 50800.0 57 45156.0 58 40477.0 59 35346.5 60 28824.5 61 23200.0 62 18373.5 63 13497.0 64 9572.0 65 7138.0 66 5375.0 67 4073.0 68 3008.5 69 2077.0 70 1435.0 71 962.5 72 722.0 73 598.0 74 455.0 75 361.0 76 266.0 77 171.5 78 105.5 79 68.0 80 43.0 81 35.0 82 23.0 83 13.0 84 10.5 85 7.0 86 5.0 87 3.5 88 2.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03831962617863222 2 0.0 3 0.0 4 5.06203780431073E-5 5 0.008301741999069598 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006074445365172877 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.7841207923709016E-4 22-23 0.0011895788840130216 24-25 0.0 26-27 0.001088338127926807 28-29 0.0010630279389052535 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.290324572801974E-4 38-39 0.0 40-41 2.5310189021553653E-4 42-43 0.022348896906031875 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.024815121724292E-4 84-85 3.037222682586438E-4 86-87 0.0 88-89 0.0 90-91 0.011035242413397392 92-93 0.0023538475790044894 94-95 0.0 96-97 0.0 98-99 6.074445365172876E-4 100-101 0.013490330748488094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1975489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.93830464719288 #Duplication Level Percentage of deduplicated Percentage of total 1 78.0036354943978 47.53409303346201 2 13.02786651366301 15.877921970251183 3 3.9858562029871694 7.286739587326068 4 1.7477073663953056 4.260092957101611 5 0.9115053426098838 2.7772795127752508 6 0.5616524932472858 2.053569044361513 7 0.3456317067606924 1.4743547169618616 8 0.24281956899035181 1.1837610295547303 9 0.17207059036921332 0.943712105106731 >10 0.926022393940131 9.983344319248559 >50 0.049095489328237076 2.0396184398734203 >100 0.024472908022927217 2.6875129801756157 >500 0.0012479469661978541 0.4933472488792254 >1k 1.663929288263797E-4 0.19123479835293705 >5k 2.495893932395695E-4 1.213418256569246 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9775 0.4948141953713739 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8383 0.42435062913536853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5776 0.2923833035769878 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2235 0.11313654492634481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 5.06203780431073E-5 0.0 0.0 0.0 2.531018902155365E-5 14-15 5.06203780431073E-5 0.0 0.0 0.0 5.06203780431073E-5 16-17 5.06203780431073E-5 0.0 0.0 0.0 5.06203780431073E-5 18-19 7.593056706466095E-5 0.0 0.0 0.0 1.012407560862146E-4 20-21 1.012407560862146E-4 0.0 0.0 0.0 1.012407560862146E-4 22-23 1.012407560862146E-4 0.0 0.0 5.06203780431073E-5 1.012407560862146E-4 24-25 1.012407560862146E-4 0.0 0.0 1.518611341293219E-4 1.012407560862146E-4 26-27 1.012407560862146E-4 0.0 0.0 2.2779170119398287E-4 1.012407560862146E-4 28-29 2.024815121724292E-4 0.0 0.0 5.568241584741803E-4 1.012407560862146E-4 30-31 2.5310189021553653E-4 0.0 0.0 0.0020754354997673994 1.012407560862146E-4 32-33 2.5310189021553653E-4 0.0 0.0 0.004277421944642567 1.012407560862146E-4 34-35 2.5310189021553653E-4 0.0 0.0 0.006251616688323752 1.012407560862146E-4 36-37 2.5310189021553653E-4 0.0 0.0 0.008757325401457563 1.2655094510776826E-4 38-39 3.037222682586438E-4 0.0 0.0 0.013237228858272559 1.518611341293219E-4 40-41 3.037222682586438E-4 0.0 0.0 0.017590581369979787 1.518611341293219E-4 42-43 3.037222682586438E-4 0.0 0.0 0.022374207095053427 1.518611341293219E-4 44-45 3.037222682586438E-4 0.0 0.0 0.024702744485036364 1.518611341293219E-4 46-47 3.037222682586438E-4 0.0 0.0 0.027815897734687463 1.518611341293219E-4 48-49 3.037222682586438E-4 0.0 0.0 0.032903245728019746 1.518611341293219E-4 50-51 3.037222682586438E-4 0.0 0.0 0.038699279013955534 1.518611341293219E-4 52-53 3.037222682586438E-4 0.0 0.0 0.04970921123833137 1.518611341293219E-4 54-55 3.037222682586438E-4 0.0 0.0 0.058998050609241556 1.518611341293219E-4 56-57 3.037222682586438E-4 0.0 0.0 0.07782883124127749 1.518611341293219E-4 58-59 3.037222682586438E-4 0.0 0.0 0.10567003916498649 1.518611341293219E-4 60-61 3.290324572801975E-4 0.0 0.0 0.13254945990587647 1.518611341293219E-4 62-63 3.543426463017511E-4 0.0 0.0 0.14824177709923975 2.024815121724292E-4 64-65 3.543426463017511E-4 0.0 0.0 0.17522243859621592 2.024815121724292E-4 66-67 4.049630243448584E-4 0.0 0.0 0.2249316498345473 2.024815121724292E-4 68-69 4.5558340238796574E-4 0.0 0.0 0.2933957111378499 2.024815121724292E-4 70-71 4.5558340238796574E-4 0.0 0.0 0.3576076606855315 2.024815121724292E-4 72-73 4.5558340238796574E-4 0.0 0.0 0.4306275560127138 2.024815121724292E-4 74-75 4.5558340238796574E-4 0.0 0.0 0.5168846801981687 2.024815121724292E-4 76-77 4.5558340238796574E-4 0.0 2.531018902155365E-5 0.585247500745385 2.024815121724292E-4 78-79 4.5558340238796574E-4 0.0 5.06203780431073E-5 0.6738078521318013 2.024815121724292E-4 80-81 5.568241584741803E-4 0.0 5.06203780431073E-5 0.7891210733139997 2.024815121724292E-4 82-83 5.568241584741803E-4 0.0 5.06203780431073E-5 0.8938293252961671 2.024815121724292E-4 84-85 5.568241584741803E-4 0.0 5.06203780431073E-5 1.0156472650569048 2.024815121724292E-4 86-87 5.568241584741803E-4 0.0 5.06203780431073E-5 1.1732031917160763 2.024815121724292E-4 88-89 5.568241584741803E-4 0.0 5.06203780431073E-5 1.3784182043028332 2.024815121724292E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5470 0.0 45.518764 1 GTATCAA 9320 0.0 38.441643 1 ATCAACG 11875 0.0 29.882198 3 TATCAAC 12125 0.0 29.344425 2 TCAACGC 12120 0.0 29.278145 4 CAACGCA 12325 0.0 28.75262 5 AACGCAG 12560 0.0 28.286716 6 ACGCAGA 14200 0.0 24.98888 7 CGCAGAG 14350 0.0 24.661469 8 GTACATG 7785 0.0 24.658648 1 ACATGGG 7480 0.0 24.005775 3 TACATGG 7795 0.0 23.706041 2 GAGTACT 9595 0.0 22.67436 12-13 GCAGAGT 15620 0.0 22.32182 9 AGTACTT 10035 0.0 21.39615 12-13 GTACTTT 10215 0.0 21.202988 14-15 TGGTATC 2975 0.0 20.757828 2 AGAGTAC 14180 0.0 20.652441 10-11 CATGGGA 4150 0.0 20.374992 4 CATGGGG 3505 0.0 19.651943 4 >>END_MODULE