FastQCFastQC Report
Thu 26 May 2016
SRR937580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745095
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70000.40112429409287176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.31304885980419406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.21792509863359874No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24120.13821597105028666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.046.9057461
GTATCAA67000.038.7489621
TATCAAC83900.030.7560352
ATCAACG83600.030.525343
TCAACGC83850.030.3820194
CAACGCA86250.029.4263955
AACGCAG87650.028.9972696
CGCAGAG101150.024.7990918
ACGCAGA102200.024.6837587
GTACATG68100.023.7396351
TACATGG66300.023.725042
GAGTACT63650.022.53727112-13
ACATGGG67950.022.3796353
GCAGAGT110500.022.1841419
GTACTTT66500.021.53474814-15
TGGTATC20600.020.3005562
GTGGTAT21600.020.0322551
AGTACTT66650.020.0262212-13
CAGAGTA108350.019.72826410-11
CATGGGA37250.019.521814
Produced by FastQC (version 0.11.5)