##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937580_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1745095 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.606753214008407 33.0 31.0 34.0 30.0 34.0 2 31.714506087061164 33.0 31.0 34.0 30.0 34.0 3 31.754866067463375 33.0 31.0 34.0 30.0 34.0 4 35.36635885152384 37.0 35.0 37.0 33.0 37.0 5 35.37083253347239 37.0 35.0 37.0 33.0 37.0 6 35.42283829820153 37.0 35.0 37.0 33.0 37.0 7 35.33700744085566 37.0 35.0 37.0 33.0 37.0 8 35.33403854804466 37.0 35.0 37.0 33.0 37.0 9 36.87544574937181 39.0 37.0 39.0 33.0 39.0 10-11 36.78914672267126 39.0 37.0 39.0 33.0 39.0 12-13 36.67441514645334 39.0 37.0 39.0 32.0 39.0 14-15 37.76822207386991 40.0 38.0 41.0 32.5 41.0 16-17 37.66365412771225 40.0 37.0 41.0 32.0 41.0 18-19 37.57508215885095 40.0 37.0 41.0 32.0 41.0 20-21 37.5831278526384 40.0 37.0 41.0 32.0 41.0 22-23 37.59563490812821 40.0 37.0 41.0 32.0 41.0 24-25 37.51918749409058 40.0 37.0 41.0 32.0 41.0 26-27 37.402808443093356 40.0 37.0 41.0 32.0 41.0 28-29 37.24239425360797 40.0 37.0 41.0 31.5 41.0 30-31 36.99294422366691 39.0 36.5 41.0 31.0 41.0 32-33 36.84663872167418 39.0 36.0 41.0 30.5 41.0 34-35 36.8073752431816 39.0 36.0 41.0 30.0 41.0 36-37 36.62864027459823 39.0 36.0 41.0 30.0 41.0 38-39 36.44400132944052 39.0 35.5 41.0 30.0 41.0 40-41 36.1940117873239 39.0 35.0 41.0 29.5 41.0 42-43 35.91832163865004 39.0 35.0 40.0 29.0 41.0 44-45 35.64305095138087 39.0 35.0 40.0 27.5 41.0 46-47 35.2685203384343 38.5 35.0 40.0 26.5 41.0 48-49 34.88992719594062 38.0 34.0 40.0 24.5 41.0 50-51 34.0277549359777 37.5 33.5 39.5 23.5 40.5 52-53 34.13976029958255 38.0 33.5 39.5 24.0 40.5 54-55 34.37906245791777 38.0 34.0 40.0 23.5 41.0 56-57 34.10088705772465 38.0 33.5 40.0 22.5 41.0 58-59 33.79085465261204 38.0 33.0 40.0 21.5 41.0 60-61 33.40559654345466 37.5 33.0 40.0 19.5 41.0 62-63 33.00202453161576 37.0 32.0 40.0 18.0 41.0 64-65 32.53314805211178 36.0 31.5 39.0 14.0 41.0 66-67 32.00719015297162 36.0 31.0 39.0 8.0 40.0 68-69 31.493671118191273 35.0 30.5 38.5 7.5 40.0 70-71 30.9921840931296 35.0 30.0 38.0 6.0 40.0 72-73 30.479598818402437 35.0 29.5 37.0 2.0 39.5 74-75 29.88626435810085 34.0 29.0 37.0 2.0 39.0 76-77 29.298202963162467 34.0 28.5 36.0 2.0 39.0 78-79 28.72007054057229 34.0 27.5 36.0 2.0 38.0 80-81 28.112947432661258 33.5 26.5 35.0 2.0 37.0 82-83 27.503626736653302 33.0 26.0 35.0 2.0 37.0 84-85 26.89194313203579 32.5 25.0 35.0 2.0 36.0 86-87 26.23719740186064 32.0 23.5 35.0 2.0 36.0 88-89 25.618397279231218 32.0 21.0 34.5 2.0 35.0 90-91 24.96218343413969 31.0 19.0 34.0 2.0 35.0 92-93 24.20655580355224 31.0 12.0 34.0 2.0 35.0 94-95 23.336630097501853 31.0 3.5 34.0 2.0 35.0 96-97 22.34997693535309 30.0 2.0 34.0 2.0 35.0 98-99 21.072651059111394 29.5 2.0 34.0 2.0 35.0 100-101 17.89485271575473 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02163227170944637 1101 2 -0.04585783531098642 1101 3 -0.10061501173169773 1101 4 -0.0898039780746629 1101 5 -0.0742037998295686 1101 6 0.031249277680892362 1101 7 0.04980725813206277 1101 8 0.010765023019111197 1101 9 0.19199076136225557 1101 10-11 0.22251224134094372 1101 12-13 0.14956603707309313 1101 14-15 0.24796479743660882 1101 16-17 0.33701681451898935 1101 18-19 0.39275670290764 1101 20-21 0.29290603179431685 1101 22-23 0.2214886191347034 1101 24-25 0.1426119233891825 1101 26-27 0.2373761025656833 1101 28-29 0.16577400531144093 1101 30-31 0.1697172785995349 1101 32-33 0.10912418011659497 1101 34-35 0.13874810075161292 1101 36-37 0.2444442693870812 1101 38-39 0.23852062756791526 1101 40-41 0.3925839081910425 1101 42-43 0.27981238121872565 1101 44-45 0.31155406536370833 1101 46-47 0.23726174568190572 1101 48-49 0.4017723537227482 1101 50-51 0.25593185759414183 1101 52-53 0.2641261802525463 1101 54-55 0.28965481351505673 1101 56-57 0.5105208822405274 1101 58-59 0.4730360365573105 1101 60-61 0.5538779015270592 1101 62-63 0.606132678201206 1101 64-65 0.621940580981132 1101 66-67 0.7736900857096742 1101 68-69 0.8943579840795017 1101 70-71 0.9471293157269578 1101 72-73 0.8970486541357374 1101 74-75 0.9830747758321827 1101 76-77 0.7255796348978727 1101 78-79 0.9058298614270761 1101 80-81 1.1624736795232522 1101 82-83 1.3310539464373292 1101 84-85 1.558861303624962 1101 86-87 1.70064491570826 1101 88-89 1.7155747410236906 1101 90-91 1.8038485134152893 1101 92-93 2.1057966661192182 1101 94-95 2.2723637884067074 1101 96-97 2.40916702681357 1101 98-99 2.4883134019913413 1101 100-101 3.1358267079638615 1102 1 0.171770497930801 1102 2 0.16791321277272075 1102 3 0.249255010013826 1102 4 0.18451636519601067 1102 5 0.13348413009570237 1102 6 0.18938827435938776 1102 7 0.22243574932662824 1102 8 0.22334496765099487 1102 9 0.28939342807549906 1102 10-11 0.3990508753029687 1102 12-13 0.3866460821361102 1102 14-15 0.48540860209758563 1102 16-17 0.5819215116876038 1102 18-19 0.4875212331568193 1102 20-21 0.6057697307454717 1102 22-23 0.5306077411240722 1102 24-25 0.49048072152456257 1102 26-27 0.5907688552227413 1102 28-29 0.5397562304576482 1102 30-31 0.7285879912335744 1102 32-33 0.851221657500183 1102 34-35 0.8245885006518634 1102 36-37 0.6880544966719384 1102 38-39 0.9499368811084778 1102 40-41 0.964405263050601 1102 42-43 0.97327620709153 1102 44-45 0.8966964931964228 1102 46-47 1.0580086815622067 1102 48-49 1.2293500617000817 1102 50-51 0.9982754397540816 1102 52-53 1.030679485892115 1102 54-55 1.173484262055986 1102 56-57 1.2753567913653967 1102 58-59 1.2806769530713993 1102 60-61 1.3751073434696224 1102 62-63 1.5002591069188753 1102 64-65 1.5039941694774512 1102 66-67 1.6205436561575084 1102 68-69 1.6379109850294746 1102 70-71 1.6202133857978076 1102 72-73 1.5258211286325398 1102 74-75 1.5862129331587553 1102 76-77 1.640860521835716 1102 78-79 1.6973265957173673 1102 80-81 1.8506694804347958 1102 82-83 2.0182268781626007 1102 84-85 2.188361418611322 1102 86-87 2.3722363463617775 1102 88-89 2.5688707013635828 1102 90-91 2.645812009854591 1102 92-93 2.6807000921305892 1102 94-95 2.752494436202028 1102 96-97 2.8832507019523526 1102 98-99 3.1796419747922684 1102 100-101 3.3897261768918305 1103 1 0.20892562929244107 1103 2 0.10615399010131199 1103 3 0.2501668918339419 1103 4 0.2168927292192322 1103 5 0.27483341122886884 1103 6 0.17807638038084406 1103 7 0.1327440667020312 1103 8 0.04404620477066601 1103 9 0.043383924240529836 1103 10-11 0.23188545994080556 1103 12-13 0.2006237106354547 1103 14-15 0.211352906382416 1103 16-17 0.32991638488537234 1103 18-19 0.38078951892031654 1103 20-21 0.31700487467082183 1103 22-23 0.24799758073419298 1103 24-25 0.14481697287972395 1103 26-27 0.31250127870065825 1103 28-29 0.2944068277402394 1103 30-31 0.38617155507036927 1103 32-33 0.45761293585717056 1103 34-35 0.42868107692064683 1103 36-37 0.44227068358744503 1103 38-39 0.46420783159329204 1103 40-41 0.48946073586132854 1103 42-43 0.36398565114757986 1103 44-45 0.6744255528436298 1103 46-47 0.6818751617997023 1103 48-49 0.4448287489955405 1103 50-51 0.45609102685399705 1103 52-53 0.5746283901141354 1103 54-55 0.6197802487627797 1103 56-57 0.6399539929014111 1103 58-59 0.7625061880025541 1103 60-61 0.85330526235267 1103 62-63 1.0289767984393876 1103 64-65 0.9525437707824622 1103 66-67 1.284366518047939 1103 68-69 1.33203137369377 1103 70-71 1.3735047725674185 1103 72-73 1.3486837789663895 1103 74-75 1.5162893285912382 1103 76-77 1.5440969423956084 1103 78-79 1.5227858295339978 1103 80-81 1.6800827351436531 1103 82-83 1.8701567747146761 1103 84-85 2.1666966263683953 1103 86-87 2.2748726980239375 1103 88-89 2.3674945197167254 1103 90-91 2.337194473353989 1103 92-93 2.424844291042408 1103 94-95 2.6906840468284905 1103 96-97 2.8161255613705762 1103 98-99 3.225237585601107 1103 100-101 3.5928936997142067 1104 1 0.17908919387225097 1104 2 0.16803376677175308 1104 3 0.16197086271209926 1104 4 0.14384388752139188 1104 5 0.059868364684632525 1104 6 0.1805145844303766 1104 7 0.29779268072377363 1104 8 0.2825705531771945 1104 9 0.4339514069190358 1104 10-11 0.41211877619843307 1104 12-13 0.37187287625428667 1104 14-15 0.4314515315292411 1104 16-17 0.48040588849973176 1104 18-19 0.6384029559497648 1104 20-21 0.573443710004085 1104 22-23 0.5604967398849681 1104 24-25 0.49356553049987895 1104 26-27 0.4975737469683068 1104 28-29 0.4349230832963187 1104 30-31 0.5839346373908043 1104 32-33 0.46259981860915644 1104 34-35 0.6409916271206342 1104 36-37 0.5170532270399448 1104 38-39 0.5485424223196347 1104 40-41 0.6122899218675286 1104 42-43 0.6548930956385917 1104 44-45 0.812813036867496 1104 46-47 0.687733940524403 1104 48-49 0.7978232071522982 1104 50-51 0.7465014647682722 1104 52-53 0.6885815716289017 1104 54-55 0.867741771501926 1104 56-57 0.8486429407791078 1104 58-59 0.9714269515453964 1104 60-61 1.0116141463775676 1104 62-63 1.087671438219573 1104 64-65 1.100234410707536 1104 66-67 1.2226109283409698 1104 68-69 1.1082821692665057 1104 70-71 1.3695587318030427 1104 72-73 1.3121834193416397 1104 74-75 1.477328256029832 1104 76-77 1.4464427823910562 1104 78-79 1.7397127713782794 1104 80-81 1.7784240630127606 1104 82-83 1.9068861040533243 1104 84-85 2.0695668975616783 1104 86-87 2.1586207640707045 1104 88-89 2.6239036019233204 1104 90-91 2.726371838210113 1104 92-93 2.6162174366464477 1104 94-95 2.7822331239641187 1104 96-97 2.9163511676875515 1104 98-99 3.346377311458383 1104 100-101 3.8292698723754803 1105 1 0.25043035882574927 1105 2 0.28146034757799754 1105 3 0.02451616171922666 1105 4 0.11229736706891913 1105 5 0.06494499690833777 1105 6 0.19078965661689296 1105 7 0.029571477869545504 1105 8 0.17315240124196407 1105 9 0.3150788413898411 1105 10-11 0.23808975789022213 1105 12-13 0.22599491750501954 1105 14-15 0.29191178515878136 1105 16-17 0.3678502049618473 1105 18-19 0.396853999342504 1105 20-21 0.4540148704220641 1105 22-23 0.3663893716955329 1105 24-25 0.3171517187371933 1105 26-27 0.38658416293331044 1105 28-29 0.5596673993093688 1105 30-31 0.5100509463805523 1105 32-33 0.433643817585299 1105 34-35 0.5874922086543606 1105 36-37 0.5098915494386489 1105 38-39 0.49700848679578513 1105 40-41 0.6434112378850756 1105 42-43 0.6585274007394801 1105 44-45 0.7494876070040561 1105 46-47 0.6951748778040852 1105 48-49 0.7688629961835431 1105 50-51 0.7257725380684619 1105 52-53 0.8751326500483856 1105 54-55 0.9465339776028685 1105 56-57 0.9075027417553656 1105 58-59 0.9016162418946365 1105 60-61 0.9589435257306533 1105 62-63 1.0484886390440522 1105 64-65 1.0364804084532508 1105 66-67 1.3247486822287158 1105 68-69 1.1842219973660342 1105 70-71 1.2581214902936644 1105 72-73 1.317467461517431 1105 74-75 1.452549642998985 1105 76-77 1.481940476463901 1105 78-79 1.5375877034578345 1105 80-81 1.8666628241438552 1105 82-83 2.009315186128166 1105 84-85 2.182971867317434 1105 86-87 2.2523445220808256 1105 88-89 2.0902706327782887 1105 90-91 2.2499259186009724 1105 92-93 2.1979041201978013 1105 94-95 2.4023697956153605 1105 96-97 2.437337462352261 1105 98-99 2.502503960072808 1105 100-101 3.27995233251011 1106 1 -0.1240530153924091 1106 2 -0.16774753570117795 1106 3 0.11938297565688316 1106 4 -0.0763336548804503 1106 5 -0.09019300542916397 1106 6 -0.008814445565199946 1106 7 0.05632074900657358 1106 8 0.09718882512554927 1106 9 0.14803532794922347 1106 10-11 -0.31811232017197 1106 12-13 -0.24757818070847293 1106 14-15 -0.40048797210095444 1106 16-17 -0.26724807915984883 1106 18-19 -0.0040079533680952295 1106 20-21 -0.11077109430995336 1106 22-23 -0.06242848962142489 1106 24-25 -0.12126241028761342 1106 26-27 -0.30738891087770526 1106 28-29 0.04609690188447502 1106 30-31 0.321728968753753 1106 32-33 0.22689043656014007 1106 34-35 0.13510173075142973 1106 36-37 -0.044248681228822306 1106 38-39 -0.06553421751060995 1106 40-41 0.08251248883227191 1106 42-43 0.2797949480352955 1106 44-45 0.1710831426380679 1106 46-47 0.3541569098869033 1106 48-49 0.4991405099580888 1106 50-51 0.4291045977645922 1106 52-53 0.5395275332376599 1106 54-55 0.5483975550036178 1106 56-57 0.32050556088175597 1106 58-59 -0.04251223706145879 1106 60-61 0.34080655728688924 1106 62-63 0.30135400167746695 1106 64-65 0.46782103469745095 1106 66-67 0.6695021531576728 1106 68-69 0.4584916895097244 1106 70-71 0.49651908344042184 1106 72-73 0.5373867728543011 1106 74-75 0.8507197795243364 1106 76-77 1.3479706456114862 1106 78-79 1.2664606308540947 1106 80-81 1.2941242618340993 1106 82-83 1.3157872966841602 1106 84-85 1.459045415049932 1106 86-87 1.544770287226406 1106 88-89 1.8156379540302616 1106 90-91 2.1001556549259632 1106 92-93 1.9048660459372613 1106 94-95 2.1043807510359187 1106 96-97 2.0852854080503604 1106 98-99 2.2150638424272593 1106 100-101 2.55024522625666 1107 1 -0.25444417583133117 1107 2 -0.056563365984658276 1107 3 -0.06281479473020468 1107 4 -0.17584392894565326 1107 5 -0.07556993852646343 1107 6 -0.08055873832555704 1107 7 -0.15612137537650028 1107 8 -0.08846104389383669 1107 9 -0.15852579076995 1107 10-11 -0.21436835824302847 1107 12-13 -0.08785499753761172 1107 14-15 -0.09734936805049443 1107 16-17 -0.17641370004931645 1107 18-19 0.08448197615526709 1107 20-21 -0.0888038879745352 1107 22-23 -0.13393102095895415 1107 24-25 -0.1537018625707418 1107 26-27 -0.0900581160393088 1107 28-29 -0.11541133947613247 1107 30-31 -0.28646006323032935 1107 32-33 -0.09005862971589096 1107 34-35 0.13304564528240093 1107 36-37 -0.05918874189185175 1107 38-39 0.13756653179074618 1107 40-41 0.018630691081611417 1107 42-43 0.20640079261769273 1107 44-45 0.14907267373224187 1107 46-47 0.20003559336871035 1107 48-49 0.3551050982717854 1107 50-51 0.25956097016624824 1107 52-53 0.15314935793200135 1107 54-55 0.09629208340183482 1107 56-57 0.34578712545989276 1107 58-59 0.3562492128072421 1107 60-61 0.4138261415526756 1107 62-63 0.5912563268815703 1107 64-65 0.6573895064630548 1107 66-67 0.8451594817893806 1107 68-69 0.7970174856125958 1107 70-71 0.8984180239846076 1107 72-73 0.8433934308366418 1107 74-75 0.8377907921706829 1107 76-77 1.040322236703851 1107 78-79 1.1477285740846916 1107 80-81 1.4674571816281556 1107 82-83 1.6796646409349272 1107 84-85 1.8419633799913449 1107 86-87 1.7388100728858191 1107 88-89 1.702786584817627 1107 90-91 1.8054187374795347 1107 92-93 2.098327194717559 1107 94-95 2.3717289622399704 1107 96-97 2.4658147065127913 1107 98-99 2.780927129523718 1107 100-101 3.3135017844328836 1108 1 -0.03450125608183541 1108 2 -0.006156004165305262 1108 3 -0.06541812305887262 1108 4 -0.13151520802119165 1108 5 0.09439124149274392 1108 6 0.01867433034658461 1108 7 0.08156872304861196 1108 8 0.08692771950266831 1108 9 0.193416786317691 1108 10-11 0.12019495078836684 1108 12-13 0.16230303269779256 1108 14-15 0.20709621860012106 1108 16-17 0.17419069233457662 1108 18-19 0.1455844455749542 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-1.1625299491153953 2316 64-65 -1.3703713851276191 2316 66-67 -1.4736465348889745 2316 68-69 -1.5512288537598486 2316 70-71 -1.416742667252887 2316 72-73 -1.437974103203441 2316 74-75 -1.6937515281625046 2316 76-77 -1.4526053339092435 2316 78-79 -1.360297528855785 2316 80-81 -1.7316383777164965 2316 82-83 -1.7476415591787458 2316 84-85 -2.043770484829185 2316 86-87 -2.2140791759037945 2316 88-89 -2.317050661485627 2316 90-91 -2.47584519837703 2316 92-93 -2.4446113059791124 2316 94-95 -2.382023287931588 2316 96-97 -2.717601600411559 2316 98-99 -3.0289665920461815 2316 100-101 -2.8270995792613665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7946.0 3 1762.0 4 1666.0 5 1907.0 6 2171.0 7 2565.0 8 3064.0 9 4064.0 10 4944.0 11 6097.0 12 7764.0 13 9521.0 14 11155.0 15 13471.0 16 16186.0 17 19915.0 18 18212.0 19 14440.0 20 14764.0 21 15623.0 22 17177.0 23 19250.0 24 21755.0 25 24754.0 26 28351.0 27 32275.0 28 37774.0 29 44836.0 30 53807.0 31 65310.0 32 80305.0 33 102753.0 34 134893.0 35 180818.0 36 244426.0 37 292862.0 38 173905.0 39 12607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5938574243675 18.880590665878742 14.778661252382738 22.746890657371022 2 14.870084350364293 19.52136339607897 41.608230481049894 24.000321772506837 3 19.443074925155294 24.077643124342774 29.581042022214945 26.898239928286987 4 12.48648987192887 16.383612365480495 37.47004213493637 33.65985562765426 5 13.58210833394145 37.36555077424482 34.32086010730081 14.731480784512922 6 31.931625619950033 36.18957766164352 17.499229099996498 14.379567618409952 7 28.646834859409065 32.645197296988485 20.31053971232415 18.397428131278303 8 26.021698386229897 35.88261235064702 19.38327154794225 18.71241771518083 9 25.898967022706664 17.286765039440795 20.54550434661272 36.268763591239825 10-11 24.867294953188594 27.120725415973762 26.968640034545803 21.043339596291837 12-13 25.696763592083453 24.17602484910765 27.849927974225196 22.2772835845837 14-15 22.42503555174908 25.638603586441498 26.328708040968706 25.60765282084072 16-17 21.768821732906833 29.181760398526198 26.91447086242268 22.13494700614429 18-19 21.69439589739821 27.49290358288684 29.539726156382983 21.27297436333196 20-21 22.341185646575 26.50625789970309 29.84506749931176 21.307488954410157 22-23 21.982362754676235 26.515353567426708 29.67228095338851 21.83000272450855 24-25 21.79344446594394 26.61854352470627 29.876501671123183 21.711510338226606 26-27 21.670797590292956 27.00240218496336 29.660337879215486 21.666462345528195 28-29 21.481339411789364 27.15614737365565 29.74861193338199 21.613901281173 30-31 21.665820128729276 26.80778439726738 29.777338031068563 21.749057442934777 32-33 21.416317700310515 26.905930537869967 29.661589965847472 22.016161795972042 34-35 21.918106350399942 26.95982035572254 29.54345580945441 21.578617484423106 36-37 21.857756893223506 26.783723138792332 29.490140890357235 21.868379077626923 38-39 21.608707658902375 27.07157690106139 29.570547779652617 21.74916766038362 40-41 21.937583387106773 26.904728005897088 29.472706438345785 21.68498216865035 42-43 21.710867218062337 26.927059544933503 29.36082905136156 22.0012441856426 44-45 21.782899892919787 27.184938723133058 29.14855441713054 21.883606966816618 46-47 21.90422024743787 27.348680227048206 28.737772875428096 22.00932665008583 48-49 21.993611008864537 27.412969663489783 28.565497495453457 22.027921832192224 50-51 22.072237740333335 27.601164159795488 28.429437957271077 21.8971601426001 52-53 22.099798576685142 27.663618757205228 28.323936928516975 21.912645737592655 54-55 22.048911322424786 27.66170391196984 28.32511120189924 21.964273563706136 56-57 22.013246013968192 27.870247158657097 28.24304604359003 21.873460783784683 58-59 22.124378636310283 27.593182670664973 28.286687829120787 21.995750863903954 60-61 21.94583305586619 27.63320145033032 28.514800671432212 21.906164822371277 62-63 22.002171012902085 27.6302435529757 28.506369529127852 21.861215904994364 64-65 21.898181901683763 27.639715717623552 28.502752693851075 21.959349686841605 66-67 21.819838774259566 27.67221546900272 28.360488929902537 22.147456826835175 68-69 21.872639180702418 27.634186385607183 28.430291966612703 22.062882467077692 70-71 21.90463560875679 27.657590863517363 28.32856709432216 22.109206433403685 72-73 21.97894562791082 27.59447827964101 28.243471031866324 22.18310506058184 74-75 21.872303264245225 27.885029991002984 28.13458659955939 22.108080145192396 76-77 21.99693514132053 27.719861122514537 28.134001730514065 22.14920200565087 78-79 21.983282273399535 27.756891942788375 28.139209895369238 22.12061588844285 80-81 21.860119303926464 27.905492688472073 28.113241128277554 22.121146879323906 82-83 21.899015146665963 27.96896209358044 28.083863084404147 22.048159675349446 84-85 21.96135058547586 27.817228549557715 28.015185605189764 22.20623525977666 86-87 21.966790751423186 27.756953468072787 28.121572635795022 22.154683144709004 88-89 22.045833659863643 27.7152035668879 28.02077852288884 22.218184250359613 90-91 21.978526995401186 27.700853368651874 28.096952599273823 22.223667036673124 92-93 22.00170729873078 27.527666104264632 28.17542350940019 22.295203087604403 94-95 21.848282885632774 27.626161828947893 28.087865139577346 22.437690145841987 96-97 21.97806677224792 27.499323252304457 28.010981828140245 22.511628147307377 98-99 21.915452348651126 27.478646929848484 27.86781621403765 22.738084507462737 100-101 22.269610369929868 26.7727025900716 28.58306058042126 22.37462645957727 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2191.0 1 1276.0 2 367.0 3 393.0 4 357.0 5 309.0 6 466.5 7 1063.5 8 1797.5 9 2089.5 10 1914.0 11 1723.0 12 1776.5 13 1920.5 14 2052.5 15 2186.0 16 2371.0 17 2564.5 18 2772.5 19 3020.5 20 3396.5 21 4036.0 22 4975.5 23 6091.0 24 7389.5 25 9225.5 26 11674.0 27 14395.5 28 16764.5 29 19441.0 30 22686.5 31 25791.0 32 29037.0 33 32297.5 34 35631.5 35 38811.5 36 42805.0 37 46646.0 38 49226.0 39 51544.5 40 54069.5 41 56894.0 42 59307.0 43 61720.0 44 64113.5 45 66032.5 46 66732.5 47 67618.0 48 69240.0 49 68123.0 50 65069.5 51 61867.0 52 58496.5 53 55986.0 54 53908.0 55 50056.0 56 44889.5 57 40063.0 58 36029.5 59 31454.5 60 25586.0 61 20745.5 62 16571.5 63 12226.5 64 9019.0 65 6948.5 66 5122.5 67 3737.0 68 2723.5 69 1980.0 70 1440.0 71 1014.5 72 739.5 73 589.0 74 464.5 75 339.0 76 238.0 77 171.0 78 120.0 79 73.5 80 47.0 81 33.0 82 24.0 83 13.5 84 10.5 85 11.0 86 7.0 87 6.0 88 7.5 89 8.0 90 5.5 91 4.5 92 3.0 93 3.0 94 5.0 95 3.5 96 0.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3151117847452431 2 0.2713319332185354 3 0.276489245571158 4 0.2712746297479507 5 0.27242069915964456 6 0.20766777739893816 7 0.19953068457591133 8 0.20531833510496564 9 0.20766777739893816 10-11 0.2085273294577086 12-13 0.2122807067810062 14-15 0.20740991178130702 16-17 0.19930147069357257 18-19 0.1931699993410101 20-21 0.1911930296058381 22-23 0.2003615848993894 24-25 0.19781158045837047 26-27 0.2037424896638865 28-29 0.2088138468106321 30-31 0.21136385125165108 32-33 0.20872789160475505 34-35 0.20935822978118668 36-37 0.19809809781129395 38-39 0.1901329154000212 40-41 0.1844598718121363 42-43 0.19445932742916575 44-45 0.19540483469381323 46-47 0.20208068901693033 48-49 0.20984530928115663 50-51 0.2052323798990886 52-53 0.21417172131030113 54-55 0.2037424896638865 56-57 0.17976098722419123 58-59 0.1638019706663534 60-61 0.1803340219300382 62-63 0.1748328887539074 64-65 0.18136548440056272 66-67 0.1792166042536366 68-69 0.17850031087132792 70-71 0.18082110143000812 72-73 0.19019021887060591 74-75 0.19517562081147444 76-77 0.19574865551732137 78-79 0.19202392992931616 80-81 0.19053403969411406 82-83 0.18110761878293158 84-85 0.17480423701861503 86-87 0.17996154937123765 88-89 0.18890089078245023 90-91 0.18529077213561437 92-93 0.18119357398880864 94-95 0.18196717084170203 96-97 0.1895885324294666 98-99 0.18955988069417423 100-101 0.17082164581297865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1745095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.81826764095399 #Duplication Level Percentage of deduplicated Percentage of total 1 80.41387294028806 52.122879382892584 2 11.909716039837901 15.439343235959512 3 3.480217135765461 6.7674493726403995 4 1.4671380775033824 3.803893942953958 5 0.7651812294203102 2.479886086119994 6 0.4700840549015499 1.8282020450612144 7 0.3066993024763245 1.3915802231243013 8 0.20456960686740566 1.0607878023308992 9 0.15329928910724105 0.8942934915469017 >10 0.7676183271488516 8.784274922229509 >50 0.04207011704771801 1.8494959919701026 >100 0.018738520752805344 2.2780707387509453 >500 3.534928368638538E-4 0.15036520609032145 >1k 2.6511962764789033E-4 0.43557220080411585 >5k 1.767464184319269E-4 0.7139053575252099 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7000 0.40112429409287176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5463 0.31304885980419406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3803 0.21792509863359874 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2412 0.13821597105028666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.730347058469596E-5 0.0 0.0 0.0 6 0.0 5.730347058469596E-5 0.0 0.0 0.0 7 0.0 5.730347058469596E-5 0.0 0.0 0.0 8 0.0 5.730347058469596E-5 0.0 0.0 0.0 9 0.0 5.730347058469596E-5 0.0 0.0 0.0 10-11 0.0 5.730347058469596E-5 0.0 0.0 0.0 12-13 5.730347058469596E-5 5.730347058469596E-5 0.0 0.0 0.0 14-15 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.0 0.0 16-17 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.0 0.0 18-19 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.0 0.0 20-21 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.0 0.0 22-23 1.1460694116939193E-4 5.730347058469596E-5 0.0 1.1460694116939193E-4 0.0 24-25 1.1460694116939193E-4 5.730347058469596E-5 0.0 3.4382082350817577E-4 0.0 26-27 1.1460694116939193E-4 5.730347058469596E-5 0.0 4.870794999699157E-4 0.0 28-29 1.1460694116939193E-4 5.730347058469596E-5 0.0 9.455072646474833E-4 0.0 30-31 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.0021775318822184466 0.0 32-33 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.00398259120563637 0.0 34-35 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.0056730435878849005 0.0 36-37 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.007850575470103347 0.0 38-39 1.1460694116939193E-4 5.730347058469596E-5 0.0 0.011861818411032066 0.0 40-41 1.432586764617399E-4 5.730347058469596E-5 0.0 0.01647474779310009 0.0 42-43 2.2921388233878383E-4 5.730347058469596E-5 0.0 0.019655090410550715 0.0 44-45 2.865173529234798E-4 5.730347058469596E-5 0.0 0.021517453204553335 0.0 46-47 3.4382082350817577E-4 5.730347058469596E-5 0.0 0.024210716322034045 0.0 48-49 3.4382082350817577E-4 5.730347058469596E-5 0.0 0.028594431821763286 0.0 50-51 3.4382082350817577E-4 5.730347058469596E-5 0.0 0.032978147321492526 0.0 52-53 3.4382082350817577E-4 5.730347058469596E-5 0.0 0.041802881791535705 0.0 54-55 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.049366939908715574 0.0 56-57 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.0668158467017555 0.0 58-59 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.09228723937665284 0.0 60-61 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.11795919419859663 0.0 62-63 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.13205584796243183 0.0 64-65 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.1560373504021271 2.865173529234798E-5 66-67 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.2037997931344712 5.730347058469596E-5 68-69 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.2702718190127185 5.730347058469596E-5 70-71 3.4382082350817577E-4 1.1460694116939193E-4 0.0 0.33611350671453416 5.730347058469596E-5 72-73 3.7247255880052375E-4 1.1460694116939193E-4 0.0 0.41390296803325893 5.730347058469596E-5 74-75 4.0112429409287174E-4 1.1460694116939193E-4 0.0 0.5058750383216959 5.730347058469596E-5 76-77 4.0112429409287174E-4 1.1460694116939193E-4 0.0 0.5722897607293586 5.730347058469596E-5 78-79 4.0112429409287174E-4 1.1460694116939193E-4 0.0 0.6592477773416348 5.730347058469596E-5 80-81 4.584277646775677E-4 1.1460694116939193E-4 0.0 0.7730238181875485 5.730347058469596E-5 82-83 4.584277646775677E-4 1.1460694116939193E-4 0.0 0.8823588400631484 5.730347058469596E-5 84-85 4.584277646775677E-4 1.1460694116939193E-4 0.0 1.010604007231698 5.730347058469596E-5 86-87 4.584277646775677E-4 1.1460694116939193E-4 0.0 1.1699649589277374 5.730347058469596E-5 88-89 4.584277646775677E-4 1.1460694116939193E-4 0.0 1.392245121325773 1.1460694116939193E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4065 0.0 46.905746 1 GTATCAA 6700 0.0 38.748962 1 TATCAAC 8390 0.0 30.756035 2 ATCAACG 8360 0.0 30.52534 3 TCAACGC 8385 0.0 30.382019 4 CAACGCA 8625 0.0 29.426395 5 AACGCAG 8765 0.0 28.997269 6 CGCAGAG 10115 0.0 24.799091 8 ACGCAGA 10220 0.0 24.683758 7 GTACATG 6810 0.0 23.739635 1 TACATGG 6630 0.0 23.72504 2 GAGTACT 6365 0.0 22.537271 12-13 ACATGGG 6795 0.0 22.379635 3 GCAGAGT 11050 0.0 22.184141 9 GTACTTT 6650 0.0 21.534748 14-15 TGGTATC 2060 0.0 20.300556 2 GTGGTAT 2160 0.0 20.032255 1 AGTACTT 6665 0.0 20.02622 12-13 CAGAGTA 10835 0.0 19.728264 10-11 CATGGGA 3725 0.0 19.52181 4 >>END_MODULE