FastQCFastQC Report
Thu 26 May 2016
SRR937580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745095
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91100.5220346170265802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77940.4466232497371203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52360.30004097198146806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.11311705093418982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.044.9898831
GTATCAA80100.039.93371
ATCAACG100950.031.385393
TCAACGC102250.031.0328144
TATCAAC102750.030.9742642
CAACGCA104500.030.3646415
AACGCAG106300.029.8032256
ACGCAGA120500.026.175147
CGCAGAG121750.025.750348
GTACATG66550.023.7108441
GCAGAGT131100.023.479049
TACATGG67250.023.0974222
GAGTACT82600.022.80157912-13
ACATGGG66550.022.2697093
TACGAGT1302.9416935E-421.9238054
AGTACTT86550.021.62374712-13
GTACTTT87450.021.37189914-15
AGAGTAC119200.021.24021710-11
ATAACGC1404.8520396E-420.3578173
CAGAGTA128850.019.94439510-11
Produced by FastQC (version 0.11.5)