##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1745095 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27864844034279 33.0 31.0 34.0 31.0 34.0 2 32.51009658499967 34.0 31.0 34.0 31.0 34.0 3 32.618496987270035 34.0 31.0 34.0 31.0 34.0 4 36.211870986966325 37.0 35.0 37.0 35.0 37.0 5 36.060949690418 37.0 35.0 37.0 35.0 37.0 6 36.15546316962687 37.0 35.0 37.0 35.0 37.0 7 36.12303456258828 37.0 35.0 37.0 35.0 37.0 8 36.1157352465052 37.0 35.0 37.0 35.0 37.0 9 37.80149733968638 39.0 38.0 39.0 35.0 39.0 10-11 37.75562934969156 39.0 37.0 39.0 35.0 39.0 12-13 37.67580303651091 39.0 37.0 39.0 35.0 39.0 14-15 38.94349963755555 40.0 38.0 41.0 36.0 41.0 16-17 38.91273283116392 40.0 38.0 41.0 35.5 41.0 18-19 38.894624647941804 40.0 38.0 41.0 35.0 41.0 20-21 38.818580077302386 40.0 38.0 41.0 35.0 41.0 22-23 38.67445239370923 40.0 38.0 41.0 34.5 41.0 24-25 38.62734550268037 40.0 38.0 41.0 34.0 41.0 26-27 38.5641351903478 40.0 38.0 41.0 34.0 41.0 28-29 38.457174537775884 40.0 38.0 41.0 34.0 41.0 30-31 38.510185691896424 40.0 38.0 41.0 34.0 41.0 32-33 38.42862795435205 40.0 38.0 41.0 34.0 41.0 34-35 38.29729413011899 40.0 38.0 41.0 34.0 41.0 36-37 38.14393772258816 40.0 38.0 41.0 34.0 41.0 38-39 38.005613734495824 40.0 38.0 41.0 33.5 41.0 40-41 37.826516321461014 40.0 37.0 41.0 33.0 41.0 42-43 37.626783069116584 40.0 37.0 41.0 33.0 41.0 44-45 37.335497780923106 40.0 37.0 41.0 32.5 41.0 46-47 36.97964150948802 40.0 37.0 41.0 31.5 41.0 48-49 36.64672267125859 40.0 36.0 41.0 31.0 41.0 50-51 36.3954329133944 39.0 36.0 41.0 30.0 41.0 52-53 36.17667863354144 39.0 36.0 41.0 30.0 41.0 54-55 35.97252814316699 39.0 35.0 40.0 29.5 41.0 56-57 35.695835470275256 39.0 35.0 40.0 29.0 41.0 58-59 35.43874459556643 38.0 35.0 40.0 28.0 41.0 60-61 35.095127772413534 38.0 34.5 40.0 28.0 41.0 62-63 34.732386775505056 38.0 34.0 40.0 27.0 41.0 64-65 34.33494251029313 37.0 34.0 40.0 26.0 41.0 66-67 33.916389938656636 36.5 33.5 39.0 26.0 41.0 68-69 33.4616161297809 36.0 33.0 39.0 25.0 40.0 70-71 33.051258813990074 35.5 32.5 38.5 24.5 40.0 72-73 32.60325655623333 35.0 32.0 38.0 23.5 40.0 74-75 32.11067792870875 35.0 31.5 37.0 22.0 39.0 76-77 30.889295425177426 34.0 30.5 36.0 20.5 38.5 78-79 31.439318203306982 34.5 31.0 36.0 21.5 39.0 80-81 31.23114042501984 34.5 31.0 36.0 20.5 37.5 82-83 30.86525289454156 34.0 31.0 35.5 19.5 37.0 84-85 30.45827218575493 34.0 31.0 35.0 18.0 37.0 86-87 30.0291815058779 34.0 30.0 35.0 14.0 36.0 88-89 29.58362696586719 34.0 30.0 35.0 7.0 36.0 90-91 29.06702328526527 34.0 29.0 35.0 2.0 35.0 92-93 28.6069291356631 34.0 29.0 35.0 2.0 35.0 94-95 28.127484463596538 33.5 29.0 35.0 2.0 35.0 96-97 27.57476154593303 33.0 27.0 35.0 2.0 35.0 98-99 26.744290998484324 33.0 25.5 34.5 2.0 35.0 100-101 24.275998727862955 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1780532658593046 1101 2 0.12373634606507267 1101 3 0.12222351407448429 1101 4 0.1292523664033638 1101 5 -0.023537731532613293 1101 6 0.08584306280869924 1101 7 0.115290111717961 1101 8 0.09992026431572043 1101 9 0.23617569388186865 1101 10-11 0.10699288796663353 1101 12-13 0.18117065436219804 1101 14-15 0.25644829779280087 1101 16-17 0.12821016629387572 1101 18-19 0.1845736587839255 1101 20-21 0.22930469095239658 1101 22-23 0.16195556893010377 1101 24-25 0.05882866705972134 1101 26-27 0.18409608664963883 1101 28-29 0.07905870696479411 1101 30-31 0.17721111396657818 1101 32-33 0.19375006912041215 1101 34-35 0.03860982967682958 1101 36-37 0.14523050810304028 1101 38-39 0.20083819226115907 1101 40-41 0.2842883467224766 1101 42-43 0.18497004301161013 1101 44-45 0.22360531036810727 1101 46-47 0.3829134892942321 1101 48-49 0.27816845740212415 1101 50-51 0.48440631504711007 1101 52-53 0.3246350275795251 1101 54-55 0.22667318330884 1101 56-57 0.16273537190016185 1101 58-59 0.3298062734392673 1101 60-61 0.31641731600189615 1101 62-63 0.39728717468427277 1101 64-65 0.2316939828753064 1101 66-67 0.5614668415187722 1101 68-69 0.6812814532884417 1101 70-71 0.5740705845580152 1101 72-73 0.3548442277590951 1101 74-75 0.31144254590620335 1101 76-77 0.1712843470859191 1101 78-79 0.34601335156590807 1101 80-81 0.3694617187714506 1101 82-83 0.473620403287768 1101 84-85 0.6597060518499056 1101 86-87 0.7316826021027758 1101 88-89 0.8691467937528721 1101 90-91 0.6111439601755713 1101 92-93 0.9399692745084813 1101 94-95 0.8882655972566091 1101 96-97 1.0271196282714534 1101 98-99 1.447547724189704 1101 100-101 2.240467647502289 1102 1 0.18710395398233004 1102 2 0.17600346957206625 1102 3 0.19252069317277432 1102 4 0.12099057205331576 1102 5 0.02452317145480265 1102 6 0.02169377266753969 1102 7 0.06124943274532768 1102 8 0.05663663226043525 1102 9 0.2357562605074932 1102 10-11 0.18003227346398631 1102 12-13 0.1537768517874767 1102 14-15 0.1567235858166498 1102 16-17 0.2212326774213551 1102 18-19 0.29356386990276917 1102 20-21 0.19621069421082638 1102 22-23 0.3729806505121971 1102 24-25 0.21322211642738864 1102 26-27 0.20294827046868846 1102 28-29 0.19733867595244448 1102 30-31 0.2808915751152625 1102 32-33 0.28168419115345245 1102 34-35 0.20543102442879047 1102 36-37 0.3099339160659156 1102 38-39 0.29242689138212086 1102 40-41 0.3451172237278257 1102 42-43 0.3672502322684892 1102 44-45 0.41455957102520813 1102 46-47 0.42505732510340266 1102 48-49 0.4666762255313941 1102 50-51 0.5453925098912578 1102 52-53 0.2834380098502791 1102 54-55 0.41199311224047364 1102 56-57 0.6223072344948477 1102 58-59 0.596993095711106 1102 60-61 0.5452518200613525 1102 62-63 0.5645111855832354 1102 64-65 0.6279526323240958 1102 66-67 0.7106828722671921 1102 68-69 0.6218091242469654 1102 70-71 0.7332346337081361 1102 72-73 0.6679747726974128 1102 74-75 0.6537072144301135 1102 76-77 0.5557497381317305 1102 78-79 0.7899031606691409 1102 80-81 0.8444711068984567 1102 82-83 0.7198226997158201 1102 84-85 0.7543702722666303 1102 86-87 0.7786138975090502 1102 88-89 0.7981024458905033 1102 90-91 1.0684319850009807 1102 92-93 1.2226342556788588 1102 94-95 1.1122387738979782 1102 96-97 1.1631367287326597 1102 98-99 1.3801757835789452 1102 100-101 2.0768978498103614 1103 1 0.23286572992283538 1103 2 0.18267388508621707 1103 3 0.20759055345221356 1103 4 0.11942162872170314 1103 5 0.013090199834593363 1103 6 0.02666960779930605 1103 7 0.09795571561106442 1103 8 0.07275414343098419 1103 9 0.13764942848625594 1103 10-11 0.154280224262898 1103 12-13 0.21621466651335197 1103 14-15 0.26541483276437816 1103 16-17 0.16873108162816663 1103 18-19 0.16347151653842928 1103 20-21 0.15894867003582647 1103 22-23 0.2776274482871983 1103 24-25 0.23316243882827337 1103 26-27 0.20819665694445888 1103 28-29 0.22830567596257367 1103 30-31 0.1544832243041867 1103 32-33 0.2995314914766567 1103 34-35 0.3415400247023612 1103 36-37 0.26694020805047813 1103 38-39 0.20558205313982114 1103 40-41 0.22595478717960304 1103 42-43 0.28054217629921396 1103 44-45 0.041841048679032156 1103 46-47 0.20583460303485168 1103 48-49 0.2187340388387966 1103 50-51 0.2069455459511218 1103 52-53 0.07155948264074397 1103 54-55 0.049119873945493 1103 56-57 0.2539520666579378 1103 58-59 0.18671162818930043 1103 60-61 0.1339140894312436 1103 62-63 0.2032365267790155 1103 64-65 0.24426847779051997 1103 66-67 0.1915503251786319 1103 68-69 0.16656656368280665 1103 70-71 0.2087361687092013 1103 72-73 0.15886380681192236 1103 74-75 0.4899327329362535 1103 76-77 0.43570606912457066 1103 78-79 0.37523756799662067 1103 80-81 0.30791528453303485 1103 82-83 0.3442925417576248 1103 84-85 0.4766442522254941 1103 86-87 0.6920492096259316 1103 88-89 0.7406108301372356 1103 90-91 0.733141398660969 1103 92-93 0.9767911702760586 1103 94-95 0.7410696907444887 1103 96-97 0.9845596189927477 1103 98-99 1.3395344773588995 1103 100-101 2.144192701730887 1104 1 0.13089374443200796 1104 2 0.13489760790091054 1104 3 0.040618076387993085 1104 4 0.03968376670377438 1104 5 -0.04578269998275175 1104 6 8.821848340332394E-4 1104 7 0.008648213166132734 1104 8 0.003682906684062459 1104 9 0.09401375164143388 1104 10-11 0.0340719006316661 1104 12-13 0.04882849162706293 1104 14-15 0.0501263826694327 1104 16-17 0.03190033293601857 1104 18-19 0.10711185039438931 1104 20-21 0.10178334096624297 1104 22-23 0.06566332797073215 1104 24-25 0.08474970345516653 1104 26-27 -0.04148640757583877 1104 28-29 -0.04288424212577269 1104 30-31 -0.025323975435014745 1104 32-33 -0.06070824110741313 1104 34-35 -0.05607196347918375 1104 36-37 -0.08473088277676055 1104 38-39 -0.2412053853488203 1104 40-41 -0.1787105030815468 1104 42-43 -0.11420963087983438 1104 44-45 -0.04876912326815841 1104 46-47 -0.004808478457668741 1104 48-49 0.048364530877897494 1104 50-51 0.09737875147537522 1104 52-53 0.10685005426298488 1104 54-55 0.03819788923083678 1104 56-57 0.10250891065323486 1104 58-59 0.006363956436139517 1104 60-61 -0.032716097314903436 1104 62-63 -0.09707161212410398 1104 64-65 -0.07709064503556107 1104 66-67 0.10681186809816268 1104 68-69 -0.010810193692080361 1104 70-71 0.07544241997047152 1104 72-73 -0.05670354650516174 1104 74-75 0.14928944446545955 1104 76-77 0.14306440543321486 1104 78-79 0.21894568723680408 1104 80-81 0.10973721077632703 1104 82-83 0.2599473433948205 1104 84-85 0.3063572768507363 1104 86-87 0.37790690171470587 1104 88-89 0.4376230987743348 1104 90-91 0.4337113650787465 1104 92-93 0.8183640499130185 1104 94-95 1.0346988995182969 1104 96-97 1.0592291005639183 1104 98-99 1.1857214201345805 1104 100-101 1.8833674793586077 1105 1 0.24534979709785887 1105 2 0.19110542425300991 1105 3 0.12785487063283085 1105 4 0.09969175998582358 1105 5 -0.048331119962362834 1105 6 -0.017356980451772586 1105 7 0.15810967701449385 1105 8 0.1484881714727564 1105 9 0.1779555623443443 1105 10-11 0.18575705737634252 1105 12-13 0.14597802213400257 1105 14-15 0.25873981767012566 1105 16-17 0.21298100895212713 1105 18-19 0.15693514497377947 1105 20-21 0.28144386223601003 1105 22-23 0.3240526255049403 1105 24-25 0.21839019436842477 1105 26-27 0.05475237151768653 1105 28-29 0.11566172149131404 1105 30-31 0.0060535858954580135 1105 32-33 0.06820476234586437 1105 34-35 0.03291757115911764 1105 36-37 0.11064087219770613 1105 38-39 0.05580706935216995 1105 40-41 0.07584409597451724 1105 42-43 0.07112112484457356 1105 44-45 0.2141509169676823 1105 46-47 0.33080804832656696 1105 48-49 0.08362899400326995 1105 50-51 0.11702347703186433 1105 52-53 -0.03967567467494604 1105 54-55 -0.037957207384593517 1105 56-57 0.2087120679996488 1105 58-59 0.15183495384506074 1105 60-61 0.27061716865931373 1105 62-63 0.2668031874825232 1105 64-65 0.31530260198828586 1105 66-67 0.39491243077132765 1105 68-69 0.34525375480961884 1105 70-71 0.42408311296107826 1105 72-73 0.528133859809536 1105 74-75 0.7237785255523619 1105 76-77 0.5177480982397071 1105 78-79 0.4764711040774827 1105 80-81 0.6646671685197028 1105 82-83 0.594655083406586 1105 84-85 0.6046130686122666 1105 86-87 0.783633044237618 1105 88-89 0.8988475416021622 1105 90-91 0.9026633021024608 1105 92-93 1.0873750384305332 1105 94-95 1.1497617455757663 1105 96-97 1.176549880402888 1105 98-99 1.4559092285832094 1105 100-101 1.9400476515285128 1106 1 -0.1517992890941997 1106 2 0.05017029626852576 1106 3 -0.06762810543990838 1106 4 -0.0022000728958531113 1106 5 -0.12180050771746664 1106 6 -0.12855674670188222 1106 7 -0.13720729810783894 1106 8 -0.090367609983943 1106 9 -0.0760798340672082 1106 10-11 -0.0340890465781456 1106 12-13 -0.18556938285694002 1106 14-15 -0.12372251905814835 1106 16-17 -0.06383392406954158 1106 18-19 -0.055209574462146804 1106 20-21 -0.013382655532829801 1106 22-23 -0.00657583418604446 1106 24-25 -0.12940138292957926 1106 26-27 -0.1751075712007477 1106 28-29 -0.13937128604775495 1106 30-31 -0.10334039825578145 1106 32-33 0.04559267156870561 1106 34-35 -0.3087700195473957 1106 36-37 -0.10022913974665215 1106 38-39 -0.1429765000856662 1106 40-41 -0.1752180532401013 1106 42-43 -0.2150683666852089 1106 44-45 -0.08947638039556693 1106 46-47 -0.09426307200325112 1106 48-49 -0.052881743079488785 1106 50-51 -0.21119315260299487 1106 52-53 -0.0923088928457858 1106 54-55 -0.2635534622490283 1106 56-57 -0.3493296666300125 1106 58-59 -0.143626830616995 1106 60-61 -0.04358217375858686 1106 62-63 0.04349418169014285 1106 64-65 -0.28708594940457743 1106 66-67 0.0047793731429877084 1106 68-69 -0.31684199916220024 1106 70-71 -0.09561481026106833 1106 72-73 0.12070764514493959 1106 74-75 -0.11300047805663382 1106 76-77 -0.12056585226182648 1106 78-79 0.19452394824850217 1106 80-81 0.24964472467747356 1106 82-83 0.14970462682369856 1106 84-85 0.2129579074918091 1106 86-87 0.11052311115173197 1106 88-89 0.2519233754599348 1106 90-91 0.5222565516111999 1106 92-93 0.6769243635098441 1106 94-95 0.5450514284722132 1106 96-97 0.8889246572488041 1106 98-99 1.0359841377161523 1106 100-101 1.8482402961130937 1107 1 0.10156063799564663 1107 2 0.08072512282856792 1107 3 0.002789680566237962 1107 4 -0.011106118362903317 1107 5 -0.17871846311566486 1107 6 -0.05021968356238915 1107 7 -0.07255170475055195 1107 8 -0.04705859594110251 1107 9 0.13082945291155568 1107 10-11 -0.02904466268009287 1107 12-13 -0.0642612945058616 1107 14-15 -0.03983909896109594 1107 16-17 -0.07119555939470246 1107 18-19 0.03430211517981263 1107 20-21 0.04508495877498575 1107 22-23 0.07964710483758353 1107 24-25 0.04068748029866498 1107 26-27 -0.10640438265387075 1107 28-29 -0.005786170916827871 1107 30-31 -0.1145495393824234 1107 32-33 -0.10293568529660035 1107 34-35 0.04402797128794589 1107 36-37 -0.059442715286763814 1107 38-39 -0.06772555331930619 1107 40-41 -0.04120301631581924 1107 42-43 0.0734078842313366 1107 44-45 0.16957082511277832 1107 46-47 0.07537248855718559 1107 48-49 0.005879325060654139 1107 50-51 0.08696484803004267 1107 52-53 0.03817117853745344 1107 54-55 0.015686707047585458 1107 56-57 0.06420086131649327 1107 58-59 -0.036001961637367685 1107 60-61 0.09949684882543153 1107 62-63 -0.1279504541487526 1107 64-65 -0.04817587988226535 1107 66-67 0.035372690666399365 1107 68-69 -0.07107719803482837 1107 70-71 0.05263552096798918 1107 72-73 0.05914946096408613 1107 74-75 0.1499250896453148 1107 76-77 0.09474702996377715 1107 78-79 0.08136687807884257 1107 80-81 0.011128789895796132 1107 82-83 0.04447780848355265 1107 84-85 -0.014532593033862895 1107 86-87 0.2762303666983321 1107 88-89 0.485272296042897 1107 90-91 0.5569618810190917 1107 92-93 0.8059810645161747 1107 94-95 0.6483780338396343 1107 96-97 0.9270520241647091 1107 98-99 1.2586883982840682 1107 100-101 2.1146472283037845 1108 1 0.04838222388880098 1108 2 0.07301523117283182 1108 3 0.0020809115789361954 1108 4 0.037046889171151065 1108 5 -0.12546610618825582 1108 6 -0.0371191799396442 1108 7 -0.04393853841978057 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-0.3453959918209719 2316 84-85 -0.33159873049439526 2316 86-87 -0.11315604692264358 2316 88-89 -0.27629962105574535 2316 90-91 -0.41635540885242506 2316 92-93 -0.2967014631274303 2316 94-95 -0.29213836033529716 2316 96-97 -0.37116209522221766 2316 98-99 -1.0158184648579756 2316 100-101 -1.2784040898642957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 12.0 5 31.0 6 70.0 7 127.0 8 321.0 9 706.0 10 1129.0 11 1881.0 12 3123.0 13 4552.0 14 6330.0 15 8849.0 16 11977.0 17 17286.0 18 15922.0 19 9714.0 20 8026.0 21 7694.0 22 8132.0 23 8710.0 24 10277.0 25 12227.0 26 14909.0 27 18332.0 28 22997.0 29 28754.0 30 36442.0 31 46758.0 32 60267.0 33 80376.0 34 112862.0 35 165889.0 36 261608.0 37 405994.0 38 324020.0 39 28780.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78548066697434 19.47726732003474 15.39923006142883 22.33802195156209 2 15.402895202206873 20.015827227645044 40.925932929687455 23.65534464046063 3 19.680705061902074 24.063274492219623 29.996819657382552 26.259200788495757 4 12.897306905706458 16.974510814036844 37.27915933420167 32.84902294605502 5 13.586012113542683 37.47942485889694 34.353035033905996 14.581527993654376 6 32.064500786490136 36.45755675192468 17.08296682988605 14.394975631699133 7 28.59168125517522 32.98038215684533 20.14801486452027 18.279921723459182 8 26.308137952375088 35.93110976766308 19.083373684527203 18.677378595434632 9 25.99124976004172 17.1823883513505 20.40828722791596 36.41807466069182 10-11 24.972394053045825 27.19129904102642 26.81653434340251 21.019772562525247 12-13 25.87028097870203 24.183629408280854 27.882916324145405 22.06317328887171 14-15 22.513559433727103 25.66315873920904 26.48377308971718 25.339508737346677 16-17 21.697185540042234 29.07048040364565 27.22115414920105 22.011179907111075 18-19 21.608737633194757 27.177202387262582 30.109879404846158 21.104180574696507 20-21 22.119588434659804 26.188969244341347 30.690647807696266 21.00079451330258 22-23 21.947473814410305 26.140778291568097 30.41725892309945 21.494488970922156 24-25 21.666637537293955 26.26190591480031 30.7046318854316 21.366824662474137 26-27 21.5432336826808 26.63577188903782 30.458441901524473 21.362552526756915 28-29 21.40632283977377 26.741084590767795 30.550621771411723 21.30197079804671 30-31 21.54596215205193 26.514278936076575 30.53227115194191 21.407487759929587 32-33 21.387603540208413 26.494145017893008 30.46771092691229 21.65054051498629 34-35 21.79064750056587 26.545001848036925 30.33608485497924 21.328265796417963 36-37 21.729676607863755 26.423260624779736 30.28517072136474 21.561892045991765 38-39 21.595299969342644 26.658262157647577 30.363246700036388 21.38319117297339 40-41 21.872820677384325 26.52560462324401 30.24560267492601 21.35597202444566 42-43 21.645989830406485 26.502321756310863 30.152543902757024 21.69914451052563 44-45 21.791163231801132 26.833467518960287 29.810182253688193 21.565186995550388 46-47 21.89803420444159 27.098037642649825 29.31828926218916 21.685638890719417 48-49 22.024359705345553 27.14104389732364 29.140247379082513 21.69434901824829 50-51 22.100917142046708 27.34954257504606 28.909514954773236 21.640025328133998 52-53 22.23472711649713 27.448714095425782 28.660797452516178 21.65576133556091 54-55 22.08043115131268 27.466241092891792 28.769035496634853 21.684292259160674 56-57 22.03163724610981 27.64319421005733 28.727834301284457 21.5973342425484 58-59 22.154696449190446 27.333640861958802 28.73370790701939 21.77795478183136 60-61 22.032726012050922 27.393924113013906 28.96959764368129 21.603752231253885 62-63 22.01476137402262 27.441142172775695 28.9665605597403 21.577535893461388 64-65 21.92677189493982 27.334242548399946 28.98168867597466 21.75729688068558 66-67 21.87007011079626 27.475954031155897 28.812557482543934 21.841418375503913 68-69 22.016566433346036 27.30645036516637 28.912809904331855 21.764173297155743 70-71 21.98579446964205 27.422633151776836 28.73983937837195 21.85173300020916 72-73 22.048188248939095 27.40615330926395 28.625772773779744 21.919885668017216 74-75 21.971194674900037 27.640803303432364 28.468151285833315 21.919850735834288 76-77 22.037424896638864 27.41077133336581 28.68505726049298 21.866746509502345 78-79 22.05871313596108 27.47712875230288 28.56128176403004 21.902876347706 80-81 21.943303946203503 27.71989490543495 28.48821983903455 21.848581309327 82-83 21.984422029204755 27.76199542431132 28.44763243209171 21.805950114392218 84-85 22.020475167606016 27.66583031987758 28.353130312227538 21.960564200288868 86-87 21.951813511585332 27.663221773026685 28.435529297831923 21.949435417556064 88-89 21.98986301605357 27.646403204410074 28.322756067721244 22.040977711815117 90-91 21.92079254447948 27.579024643516593 28.449272820840797 22.05090999116313 92-93 21.9558982931283 27.368284122749436 28.56763003825999 22.108187545862272 94-95 21.796148633742003 27.443061839040283 28.58483349044035 22.175956036777368 96-97 21.87946787997215 27.37578756457385 28.480197353152693 22.26454720230131 98-99 21.799687867369705 27.282065152457623 28.3453618334626 22.57288514671007 100-101 21.660856819871828 27.50024715131248 28.618975776305856 22.21992025250984 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 325.0 1 243.0 2 261.0 3 387.5 4 320.0 5 254.0 6 538.0 7 1268.0 8 2100.0 9 2568.0 10 2423.5 11 2104.5 12 2153.0 13 2361.0 14 2513.0 15 2693.0 16 2880.0 17 3065.0 18 3285.0 19 3540.0 20 3835.5 21 4365.0 22 5245.0 23 6380.5 24 7646.5 25 9450.0 26 11793.0 27 14361.0 28 16777.5 29 19453.5 30 22884.0 31 25932.0 32 28974.5 33 32227.0 34 35690.0 35 39106.5 36 42849.5 37 46615.5 38 49380.0 39 51822.5 40 54315.0 41 56688.5 42 59242.5 43 62024.0 44 64653.5 45 66236.5 46 66763.0 47 68173.0 48 70253.5 49 68524.5 50 64635.0 51 61488.0 52 57969.5 53 55628.5 54 53973.5 55 49992.5 56 44469.5 57 39691.0 58 35607.0 59 31009.5 60 25154.0 61 20079.5 62 15908.5 63 11642.0 64 8272.5 65 6150.5 66 4553.5 67 3438.5 68 2481.5 69 1688.0 70 1181.0 71 815.0 72 607.5 73 527.0 74 424.5 75 319.0 76 218.5 77 140.5 78 102.0 79 58.0 80 27.0 81 19.0 82 12.5 83 12.0 84 7.5 85 2.5 86 2.5 87 1.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04584277646775677 2 5.730347058469596E-5 3 0.0 4 1.1460694116939193E-4 5 0.015529240528452606 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00398259120563637 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.595520587704394E-5 22-23 2.0056214704643587E-4 24-25 3.7247255880052375E-4 26-27 0.0018337110587102708 28-29 0.0023207905586801864 30-31 4.0112429409287174E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010744400734630493 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.865173529234798E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.595520587704394E-5 74-75 5.730347058469596E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.432586764617399E-4 84-85 3.151690882158278E-4 86-87 0.0 88-89 0.0 90-91 0.007621361587764563 92-93 0.0034668599703741054 94-95 0.0 96-97 0.0 98-99 2.0056214704643587E-4 100-101 0.012176987499247892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1745095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.1205177095045 #Duplication Level Percentage of deduplicated Percentage of total 1 78.72162874610937 48.902283326437264 2 12.743218671400516 15.832306823056486 3 3.8798848391571337 7.230613645850963 4 1.6161215497368406 4.01577229404557 5 0.8524139974102519 2.647619941097654 6 0.5134862933036846 1.9138820626055617 7 0.335713030301007 1.4598267070887587 8 0.23047255591604987 1.1453659593070196 9 0.1704223604392832 0.9528052733787645 >10 0.8665448234908523 9.506623632445883 >50 0.0458480876237959 1.956239806400382 >100 0.02257966613022796 2.515401002100182 >500 0.001202773708396118 0.4588193968391381 >1k 1.8504210898401816E-4 0.18995341432460872 >5k 2.775631634760272E-4 1.2724867150217358 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9110 0.5220346170265802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7794 0.4466232497371203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5236 0.30004097198146806 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.11311705093418982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 2.865173529234798E-5 0.0 0.0 0.0 0.0 20-21 5.730347058469596E-5 0.0 0.0 0.0 0.0 22-23 5.730347058469596E-5 0.0 0.0 5.730347058469596E-5 0.0 24-25 5.730347058469596E-5 0.0 0.0 2.865173529234798E-4 2.865173529234798E-5 26-27 5.730347058469596E-5 0.0 0.0 4.297760293852197E-4 5.730347058469596E-5 28-29 8.595520587704394E-5 0.0 0.0 8.882037940627875E-4 5.730347058469596E-5 30-31 1.1460694116939193E-4 0.0 0.0 0.0021202284116337506 5.730347058469596E-5 32-33 1.1460694116939193E-4 0.0 0.0 0.003953939470344022 5.730347058469596E-5 34-35 1.1460694116939193E-4 0.0 0.0 0.0056730435878849005 5.730347058469596E-5 36-37 1.1460694116939193E-4 0.0 0.0 0.007850575470103347 5.730347058469596E-5 38-39 1.1460694116939193E-4 0.0 0.0 0.01177586320515502 5.730347058469596E-5 40-41 1.1460694116939193E-4 0.0 0.0 0.016216882175468957 5.730347058469596E-5 42-43 1.1460694116939193E-4 0.0 0.0 0.01942587652821193 5.730347058469596E-5 44-45 1.1460694116939193E-4 0.0 0.0 0.021259587586922202 1.1460694116939193E-4 46-47 2.0056214704643587E-4 0.0 0.0 0.023809592027941175 1.7191041175408788E-4 48-49 2.2921388233878385E-4 0.0 0.0 0.028193307527670415 1.7191041175408788E-4 50-51 2.2921388233878385E-4 0.0 0.0 0.0326629782332767 1.7191041175408788E-4 52-53 2.2921388233878385E-4 0.0 0.0 0.04171692658565866 1.7191041175408788E-4 54-55 2.2921388233878385E-4 0.0 0.0 0.049366939908715574 1.7191041175408788E-4 56-57 2.865173529234798E-4 0.0 0.0 0.0666439362900014 1.7191041175408788E-4 58-59 2.865173529234798E-4 0.0 0.0 0.09194341855314467 1.7191041175408788E-4 60-61 2.865173529234798E-4 0.0 0.0 0.11793054246330428 1.7191041175408788E-4 62-63 3.151690882158278E-4 0.0 0.0 0.1320271962271395 1.7191041175408788E-4 64-65 3.4382082350817577E-4 0.0 0.0 0.15595139519625006 1.7191041175408788E-4 66-67 3.4382082350817577E-4 0.0 0.0 0.2039717035462253 1.7191041175408788E-4 68-69 3.4382082350817577E-4 0.0 0.0 0.2709021571891502 1.7191041175408788E-4 70-71 3.7247255880052375E-4 0.0 0.0 0.33740283480268984 1.7191041175408788E-4 72-73 4.0112429409287174E-4 0.0 0.0 0.41596589297430797 2.2921388233878385E-4 74-75 4.0112429409287174E-4 0.0 0.0 0.508596953174469 2.2921388233878385E-4 76-77 4.0112429409287174E-4 0.0 0.0 0.5754701033468093 2.2921388233878385E-4 78-79 4.0112429409287174E-4 0.0 0.0 0.6640039654001644 2.2921388233878385E-4 80-81 4.584277646775677E-4 0.0 0.0 0.7792125930106957 2.2921388233878385E-4 82-83 4.584277646775677E-4 0.0 0.0 0.8902953707391288 2.865173529234798E-4 84-85 4.870794999699157E-4 0.0 0.0 1.020804024995774 2.865173529234798E-4 86-87 5.157312352622636E-4 0.0 0.0 1.1837750953386492 2.865173529234798E-4 88-89 5.157312352622636E-4 0.0 0.0 1.4109547044716764 2.865173529234798E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4680 0.0 44.989883 1 GTATCAA 8010 0.0 39.9337 1 ATCAACG 10095 0.0 31.38539 3 TCAACGC 10225 0.0 31.032814 4 TATCAAC 10275 0.0 30.974264 2 CAACGCA 10450 0.0 30.364641 5 AACGCAG 10630 0.0 29.803225 6 ACGCAGA 12050 0.0 26.17514 7 CGCAGAG 12175 0.0 25.75034 8 GTACATG 6655 0.0 23.710844 1 GCAGAGT 13110 0.0 23.47904 9 TACATGG 6725 0.0 23.097422 2 GAGTACT 8260 0.0 22.801579 12-13 ACATGGG 6655 0.0 22.269709 3 TACGAGT 130 2.9416935E-4 21.923805 4 AGTACTT 8655 0.0 21.623747 12-13 GTACTTT 8745 0.0 21.371899 14-15 AGAGTAC 11920 0.0 21.240217 10-11 ATAACGC 140 4.8520396E-4 20.357817 3 CAGAGTA 12885 0.0 19.944395 10-11 >>END_MODULE