FastQCFastQC Report
Thu 26 May 2016
SRR937579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166505
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110950.951131799692243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.7168421909893228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58480.5013266124020043No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.17145232982284686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17010.14582020651433125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15200.13030377066536364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.12464584378120969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55150.049.2431071
GTATCAA94650.039.3455851
TATCAAC120750.030.6742842
ATCAACG121800.030.409853
TCAACGC122950.030.1241194
CAACGCA125000.029.6300835
AACGCAG128450.028.8797266
GTATAGA2700.028.184551
GTACATG103250.028.053181
TACATGG105550.027.3435272
ACATGGG105800.026.1554053
ACGCAGA145950.025.2238317
CGCAGAG145750.025.2236848
GAGTACT77200.025.011412-13
CATGGGA76450.024.1301334
ATGGGAG27500.024.0318685
GGACCGA2002.0549123E-823.7490446
GCAGAGT150550.023.7253829
GTACTTT83000.023.0909414-15
ATGGGAT28100.022.5035365
Produced by FastQC (version 0.11.5)