##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937579_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1166505 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.851133085584717 33.0 31.0 34.0 30.0 34.0 2 31.96398643812071 33.0 31.0 34.0 30.0 34.0 3 31.963915285403836 34.0 31.0 34.0 30.0 34.0 4 35.57318657013901 37.0 35.0 37.0 33.0 37.0 5 35.583512286702586 37.0 35.0 37.0 33.0 37.0 6 35.67633829259197 37.0 35.0 37.0 33.0 37.0 7 35.62139896528519 37.0 35.0 37.0 33.0 37.0 8 35.62046026377941 37.0 35.0 37.0 33.0 37.0 9 37.2404576062683 39.0 37.0 39.0 34.0 39.0 10-11 37.130389496830276 39.0 37.0 39.0 34.0 39.0 12-13 37.015378416723465 39.0 37.0 39.0 33.0 39.0 14-15 38.127918868757526 40.0 38.0 41.0 33.0 41.0 16-17 38.065143741347015 40.0 38.0 41.0 33.0 41.0 18-19 38.08406864951286 40.0 38.0 41.0 33.0 41.0 20-21 38.03566208460315 40.0 38.0 41.0 33.0 41.0 22-23 37.94923253650863 40.0 38.0 41.0 33.0 41.0 24-25 37.96203531060733 40.0 38.0 41.0 33.0 41.0 26-27 37.87704981976074 40.0 38.0 41.0 33.0 41.0 28-29 37.74592222065058 40.0 37.5 41.0 33.0 41.0 30-31 37.45018538283162 39.5 37.0 41.0 32.5 41.0 32-33 37.23485111508309 39.0 36.0 41.0 32.0 41.0 34-35 37.19331293050608 40.0 36.0 41.0 32.0 41.0 36-37 37.0401365617807 40.0 36.0 41.0 31.5 41.0 38-39 36.86276526890155 39.5 35.5 41.0 31.0 41.0 40-41 36.59762024166206 39.0 35.0 41.0 30.5 41.0 42-43 36.25979099960995 39.0 35.0 41.0 30.0 41.0 44-45 35.897171893819575 39.0 35.0 41.0 29.5 41.0 46-47 35.37818697733829 39.0 35.0 40.5 26.5 41.0 48-49 34.91751942769213 39.0 35.0 40.0 24.0 41.0 50-51 33.98973600627515 38.0 34.0 39.5 22.5 40.5 52-53 34.0508116124663 38.0 34.0 39.5 23.0 40.5 54-55 34.311113111388295 38.0 34.0 40.0 22.5 41.0 56-57 34.104476620331674 38.0 34.0 40.0 20.5 41.0 58-59 33.86238293020604 38.0 34.0 40.0 19.5 41.0 60-61 33.54773918671587 38.0 33.5 40.0 18.0 41.0 62-63 33.193522530979294 37.0 33.0 40.0 15.5 41.0 64-65 32.80468407765076 36.5 33.0 39.5 12.0 41.0 66-67 32.36021534412626 36.0 32.0 39.0 8.0 41.0 68-69 31.873343449020794 35.5 31.0 39.0 6.5 40.0 70-71 31.42434665946567 35.0 31.0 38.5 2.0 40.0 72-73 30.9844462732693 35.0 31.0 37.5 2.0 40.0 74-75 30.46692856010047 35.0 30.0 37.0 2.0 39.0 76-77 29.994230629101462 34.5 29.5 36.5 2.0 39.0 78-79 29.458872872383743 34.0 29.0 36.0 2.0 38.5 80-81 28.920918898761684 34.0 29.0 35.5 2.0 37.0 82-83 28.426046180685038 34.0 28.0 35.0 2.0 37.0 84-85 27.935607648488432 34.0 27.0 35.0 2.0 36.5 86-87 27.416587155648713 33.0 26.0 35.0 2.0 36.0 88-89 26.98578703048851 33.0 25.5 35.0 2.0 35.5 90-91 26.467559933305044 33.0 25.0 35.0 2.0 35.0 92-93 25.87895722693002 32.0 23.5 35.0 2.0 35.0 94-95 25.18059116763323 32.0 19.5 34.0 2.0 35.0 96-97 24.396967865547083 31.5 12.0 34.0 2.0 35.0 98-99 23.4044526170055 31.0 2.0 34.0 2.0 35.0 100-101 20.676424018756883 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11217771467393334 1101 2 0.2224567282969332 1101 3 0.14376148870357497 1101 4 -0.017984974044921387 1101 5 0.03504007430544931 1101 6 0.10195525941947636 1101 7 0.17202702749784748 1101 8 0.1438051463748451 1101 9 0.19257105851764322 1101 10-11 0.3115190341959888 1101 12-13 0.1359279055532312 1101 14-15 0.3420704221338866 1101 16-17 0.2659157819459139 1101 18-19 0.18876074331794257 1101 20-21 0.2066731946320033 1101 22-23 0.10165957045141027 1101 24-25 0.16753462270270347 1101 26-27 0.14982030085538867 1101 28-29 0.1388358648797734 1101 30-31 0.16730486378618536 1101 32-33 0.39111877109675675 1101 34-35 0.30993073805022675 1101 36-37 0.20316699266075489 1101 38-39 0.21036511334398966 1101 40-41 0.31654273225714036 1101 42-43 0.1679790385730584 1101 44-45 0.4425692413358391 1101 46-47 0.4455653857047821 1101 48-49 0.5955120645221399 1101 50-51 0.6036647827506414 1101 52-53 0.5893212105944912 1101 54-55 0.70769791204971 1101 56-57 0.7265545192030771 1101 58-59 0.7115615201940315 1101 60-61 0.6228323135422613 1101 62-63 0.7177855034819203 1101 64-65 0.6779764494345244 1101 66-67 0.6066934454934838 1101 68-69 0.5884275667800978 1101 70-71 0.7154824283526011 1101 72-73 0.7099807987060949 1101 74-75 0.6116518525101 1101 76-77 0.7456465907559 1101 78-79 0.712028236724926 1101 80-81 0.5189618906856133 1101 82-83 0.5739000443187017 1101 84-85 0.6199945752955465 1101 86-87 0.768745340951341 1101 88-89 0.6173312504935957 1101 90-91 0.7025031273991686 1101 92-93 0.5510355980035726 1101 94-95 0.6107293187422229 1101 96-97 0.8606479046544102 1101 98-99 0.6658028033468248 1101 100-101 0.7273144661486377 1102 1 0.2585473954595976 1102 2 0.31556418712266066 1102 3 0.29331482961031696 1102 4 0.2053605554299054 1102 5 0.21706010941853293 1102 6 0.25122951523354686 1102 7 0.22651931393013314 1102 8 0.3712416698843839 1102 9 0.35049818165441593 1102 10-11 0.4156057866290652 1102 12-13 0.3755052453190473 1102 14-15 0.5181239376224909 1102 16-17 0.6579335179879706 1102 18-19 0.4231848444672366 1102 20-21 0.3811222782001664 1102 22-23 0.6098197357878092 1102 24-25 0.5510759174608282 1102 26-27 0.4638463553544412 1102 28-29 0.4899817735169023 1102 30-31 0.48461016812417057 1102 32-33 0.6311648385787976 1102 34-35 0.669664045811011 1102 36-37 0.751658949600511 1102 38-39 0.820314189535452 1102 40-41 0.6796192212437262 1102 42-43 0.8075338794084885 1102 44-45 0.905477664541948 1102 46-47 0.6807084075939116 1102 48-49 0.7641476949593766 1102 50-51 0.7137100633848448 1102 52-53 0.9377899924065076 1102 54-55 0.8599179294587174 1102 56-57 0.7361503744820865 1102 58-59 0.7214127936580823 1102 60-61 0.7342733270022777 1102 62-63 0.89921571007865 1102 64-65 0.9640285264669259 1102 66-67 0.8454467465660613 1102 68-69 0.7734517623511294 1102 70-71 0.7887530228653219 1102 72-73 0.732159457425599 1102 74-75 0.6977210188939935 1102 76-77 0.6118578692361076 1102 78-79 0.5085822735002274 1102 80-81 0.45164920964914046 1102 82-83 0.8169201507398718 1102 84-85 0.8294762146930168 1102 86-87 0.887654646902373 1102 88-89 1.138498379433365 1102 90-91 1.135822383532897 1102 92-93 1.1231320680074575 1102 94-95 1.3563460230300493 1102 96-97 1.5048672778317496 1102 98-99 1.6083801234414423 1102 100-101 1.9531333411529381 1103 1 0.12012572421645018 1103 2 0.2494019466395443 1103 3 0.21351307062031566 1103 4 0.05151732440035062 1103 5 0.11418047256486119 1103 6 0.13311956822616366 1103 7 0.17392313183008667 1103 8 0.11624511491087475 1103 9 0.13928639358433514 1103 10-11 0.22602610519269462 1103 12-13 0.22774527247395326 1103 14-15 0.3062953378231157 1103 16-17 0.4231099996026799 1103 18-19 0.37123333828138527 1103 20-21 0.3645836179101707 1103 22-23 0.4022017942924663 1103 24-25 0.3612036620830352 1103 26-27 0.533010328483229 1103 28-29 0.42444077770160504 1103 30-31 0.34280236513915696 1103 32-33 0.3977864581465411 1103 34-35 0.538725174766455 1103 36-37 0.5153225326307123 1103 38-39 0.344174797683948 1103 40-41 0.6147393665427856 1103 42-43 0.5653485167181245 1103 44-45 0.7339603076878589 1103 46-47 0.6532007576086016 1103 48-49 0.40502268102729033 1103 50-51 0.3484504958369925 1103 52-53 0.4139173519963393 1103 54-55 0.41317271956541646 1103 56-57 0.4074745818726839 1103 58-59 0.4719213150324393 1103 60-61 0.5544201459256044 1103 62-63 0.734123580950957 1103 64-65 0.8348900617328567 1103 66-67 0.8895310534571266 1103 68-69 0.7338757539209482 1103 70-71 0.6311664919056739 1103 72-73 0.621940140959385 1103 74-75 0.7649401985723898 1103 76-77 0.8097847738798016 1103 78-79 0.8829065670164873 1103 80-81 0.8328559568348552 1103 82-83 0.7512942958160167 1103 84-85 0.8098458964333624 1103 86-87 1.009308411607062 1103 88-89 1.1033103468474685 1103 90-91 1.2420704203744144 1103 92-93 0.8591651453280349 1103 94-95 0.9949671765059804 1103 96-97 1.311107335383955 1103 98-99 1.3003390263018275 1103 100-101 1.2864947497634773 1104 1 0.30472261611994256 1104 2 0.326622743840538 1104 3 0.3168417174821698 1104 4 0.26650696765612736 1104 5 0.2663307043842096 1104 6 0.20565702422341303 1104 7 0.3547985718575575 1104 8 0.270315100744142 1104 9 0.19249617936608132 1104 10-11 0.2873086511272689 1104 12-13 0.36243929239324757 1104 14-15 0.5917718822122282 1104 16-17 0.47089322704322 1104 18-19 0.49578056610829435 1104 20-21 0.45126366427404463 1104 22-23 0.5197193148986372 1104 24-25 0.39174331285146735 1104 26-27 0.41627157601478615 1104 28-29 0.5305638673384152 1104 30-31 0.49155177485840085 1104 32-33 0.624509273034505 1104 34-35 0.524048648622383 1104 36-37 0.4921634036704674 1104 38-39 0.5430994134648373 1104 40-41 0.747901339583052 1104 42-43 0.5353937805199607 1104 44-45 0.6153118628093566 1104 46-47 0.5828312418534694 1104 48-49 0.43611974464882053 1104 50-51 0.4505496147087982 1104 52-53 0.4890117969177865 1104 54-55 0.5653363668565632 1104 56-57 0.45711719370405746 1104 58-59 0.4179971553775914 1104 60-61 0.5443062217391201 1104 62-63 0.7021256683983665 1104 64-65 0.8352972213018361 1104 66-67 0.7499115430681798 1104 68-69 0.626745833125895 1104 70-71 0.6882452997531914 1104 72-73 0.8307338485664957 1104 74-75 0.7108201730293295 1104 76-77 0.5466838948980843 1104 78-79 0.9108047113884261 1104 80-81 0.9590224321074601 1104 82-83 0.6692794797689672 1104 84-85 0.7645116633690527 1104 86-87 0.9008738361799118 1104 88-89 0.9291775229443182 1104 90-91 0.9166177823560417 1104 92-93 0.9277812589195165 1104 94-95 1.127944686809066 1104 96-97 1.3568333002246895 1104 98-99 1.437123176726832 1104 100-101 1.3492825116334863 1105 1 0.17479247336817494 1105 2 0.3462450102960446 1105 3 0.14850229152651195 1105 4 0.24801825437457126 1105 5 0.21403132702490524 1105 6 0.22847588351095283 1105 7 0.29620499134816924 1105 8 0.223049594387561 1105 9 0.325448000644343 1105 10-11 0.20465384518723084 1105 12-13 0.25781477510249573 1105 14-15 0.4199650174358993 1105 16-17 0.3744584958417576 1105 18-19 0.35126982447164323 1105 20-21 0.3456832199645845 1105 22-23 0.46726502312530016 1105 24-25 0.40743373622544965 1105 26-27 0.29950610762410435 1105 28-29 0.35684752139317766 1105 30-31 0.4044207220098812 1105 32-33 0.3541371801373714 1105 34-35 0.3563668462185987 1105 36-37 0.5276391434630625 1105 38-39 0.39566699275932393 1105 40-41 0.3801989271215618 1105 42-43 0.24877601302311092 1105 44-45 0.12232843492285639 1105 46-47 0.1959953738446174 1105 48-49 0.011501030716424054 1105 50-51 -0.05913541750797435 1105 52-53 -0.07857292126679738 1105 54-55 0.09795449502941977 1105 56-57 0.01821245556026696 1105 58-59 0.07398783475017723 1105 60-61 0.04692790243509393 1105 62-63 0.09011508947268965 1105 64-65 0.1228485430573798 1105 66-67 0.12975321172353915 1105 68-69 0.11882960487493222 1105 70-71 0.12954256729343783 1105 72-73 0.20863518876261722 1105 74-75 0.06530875885395204 1105 76-77 -0.013100526610134722 1105 78-79 0.007639779401618085 1105 80-81 0.10626400639044675 1105 82-83 0.14766773257844434 1105 84-85 -0.10310905462027264 1105 86-87 0.01251255271568752 1105 88-89 -0.043449066199606534 1105 90-91 0.09967509458756751 1105 92-93 0.25283237728633523 1105 94-95 0.5375977191993968 1105 96-97 0.3515719728284985 1105 98-99 0.5565042241516593 1105 100-101 0.6323355527017362 1106 1 0.1612497986691821 1106 2 0.19201540407071604 1106 3 0.11553826729677752 1106 4 0.18919756451652603 1106 5 0.33402975817146796 1106 6 0.1982007835531192 1106 7 0.13335800509297968 1106 8 0.15260656677197204 1106 9 0.2913918130637754 1106 10-11 0.14259550948427346 1106 12-13 0.17919032768963206 1106 14-15 0.19106666179040843 1106 16-17 0.14062013752619862 1106 18-19 0.2277542954625602 1106 20-21 0.0026522856444870513 1106 22-23 0.12291300861943455 1106 24-25 0.26075687703984585 1106 26-27 0.2711690696344675 1106 28-29 0.3773753651474365 1106 30-31 0.4578933564541998 1106 32-33 0.30802684297719907 1106 34-35 0.5630979816988955 1106 36-37 0.5267608118180078 1106 38-39 0.6947402378496719 1106 40-41 0.6280988885790535 1106 42-43 0.5373503853497468 1106 44-45 0.5649506376685096 1106 46-47 0.813381814877971 1106 48-49 0.8916892780431454 1106 50-51 0.964121703238682 1106 52-53 0.8392489520196236 1106 54-55 0.8486825410752701 1106 56-57 1.0160839438364846 1106 58-59 0.9855950119529311 1106 60-61 0.9700921742117927 1106 62-63 0.9472106439605597 1106 64-65 0.9137875093790768 1106 66-67 0.9627804426444371 1106 68-69 0.9728594839902485 1106 70-71 0.8236862938784881 1106 72-73 1.114052560344799 1106 74-75 0.992870345779366 1106 76-77 0.8419641923652215 1106 78-79 0.9834473806473447 1106 80-81 0.6989723075107435 1106 82-83 0.789757958244433 1106 84-85 0.8126533214082521 1106 86-87 1.1818695264577563 1106 88-89 1.1705881744587394 1106 90-91 0.993918156748105 1106 92-93 1.205701908267038 1106 94-95 1.4571068641818172 1106 96-97 1.5414978973017845 1106 98-99 1.5470752641732624 1106 100-101 1.6640829612651125 1107 1 0.16888834960258947 1107 2 0.18105513465800982 1107 3 0.17726025051688765 1107 4 0.17047883215780502 1107 5 0.1335633471613633 1107 6 0.13554364236451022 1107 7 0.2024483478933945 1107 8 0.22473909290539495 1107 9 0.16727431486440736 1107 10-11 0.29577064720789537 1107 12-13 0.16363330135181542 1107 14-15 0.19149647627718025 1107 16-17 0.25032911730077245 1107 18-19 0.270120221763392 1107 20-21 0.2410984907018161 1107 22-23 0.23356065028497142 1107 24-25 0.20162468183243476 1107 26-27 0.24716603290168138 1107 28-29 0.16263903531154256 1107 30-31 0.14636350498158635 1107 32-33 0.14127089117559422 1107 34-35 0.2706247964387316 1107 36-37 0.14147261419677903 1107 38-39 0.0949415187279925 1107 40-41 0.22692429591003815 1107 42-43 0.2306103958615111 1107 44-45 0.34097290704227845 1107 46-47 0.6260913202409313 1107 48-49 0.7606410212445667 1107 50-51 0.42851678996076004 1107 52-53 0.5122200835919202 1107 54-55 0.39858384725969387 1107 56-57 0.49369894062454733 1107 58-59 0.5182480652320152 1107 60-61 0.26172013551290974 1107 62-63 0.43783570479254763 1107 64-65 0.5664432879311789 1107 66-67 0.47662521888005216 1107 68-69 0.6115124204719251 1107 70-71 0.4869792023287829 1107 72-73 0.6683699767859785 1107 74-75 0.39061615567210595 1107 76-77 0.6817878282687495 1107 78-79 0.7649602964948095 1107 80-81 0.6314380956012968 1107 82-83 0.38152996969057895 1107 84-85 0.2852545945000742 1107 86-87 0.19835703886748135 1107 88-89 0.4023298185770159 1107 90-91 0.5245580119193107 1107 92-93 0.7616159453696874 1107 94-95 1.1929870300341463 1107 96-97 1.4601917128786646 1107 98-99 1.637599100579017 1107 100-101 1.0546943957320316 1108 1 -0.21952180530929866 1108 2 -0.16336613432535785 1108 3 -0.08989081711909108 1108 4 0.020583502351541938 1108 5 0.021835718036406604 1108 6 0.06748441806580274 1108 7 0.035104687100947274 1108 8 -0.019380725920015607 1108 9 0.18579218492111949 1108 10-11 0.014050630460666014 1108 12-13 0.04054457908942055 1108 14-15 0.035013014167788015 1108 16-17 0.24129208308314531 1108 18-19 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2316 68-69 -0.9838736027933059 2316 70-71 -0.9604850287044258 2316 72-73 -0.7721720572495308 2316 74-75 -0.7431465812477995 2316 76-77 -0.9008290698182968 2316 78-79 -0.9835359110267916 2316 80-81 -0.9098145661729831 2316 82-83 -1.118011765170305 2316 84-85 -1.1280903538483074 2316 86-87 -1.153186174268047 2316 88-89 -1.3112615088784167 2316 90-91 -1.1996381762439334 2316 92-93 -1.4507660126402726 2316 94-95 -1.4224099043058231 2316 96-97 -1.2635620488023456 2316 98-99 -1.2465921600020877 2316 100-101 -1.4996550754328162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4185.0 3 730.0 4 684.0 5 756.0 6 880.0 7 1044.0 8 1301.0 9 1650.0 10 2125.0 11 3004.0 12 4465.0 13 6223.0 14 7211.0 15 10124.0 16 13775.0 17 19011.0 18 15482.0 19 9062.0 20 8224.0 21 8564.0 22 9145.0 23 9929.0 24 10955.0 25 12613.0 26 14112.0 27 16767.0 28 19811.0 29 23840.0 30 29017.0 31 35654.0 32 46107.0 33 59988.0 34 81985.0 35 116927.0 36 164370.0 37 219026.0 38 162027.0 39 15732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88672810790453 19.833630564923464 14.931974953437537 20.347666373734473 2 14.99424934628219 20.973897705411805 41.295366984542866 22.736485963763144 3 19.096751233953448 27.307806700397308 30.104767848270576 23.490674217378672 4 12.005432750372858 17.10946734117586 37.670708277637615 33.214391630813665 5 12.43636188573423 39.69230187290774 33.06594292729308 14.805393314064947 6 29.813695538089302 37.01344267730102 17.80693082488102 15.365930959728654 7 27.691942981416396 35.29665398899894 19.932540377856338 17.07886265172832 8 27.41846404653332 34.35740538256255 19.310950224223337 18.9131803466808 9 27.99190905850472 16.635302100466816 20.47059732964574 34.90219151138272 10-11 24.67442218043724 28.37210660724762 25.991550996812297 20.96192021550284 12-13 26.194895412295605 26.055582563047224 27.50647177755294 20.243050247104232 14-15 23.1062676852634 26.323273194669845 27.238515771314752 23.331943348752 16-17 20.377216520702415 30.474217754154743 27.473260883756566 21.67530484138628 18-19 20.484232739884334 27.532271661274994 31.889069398619164 20.09442620022151 20-21 21.80516669815696 26.433982033356806 31.95486009721912 19.805991171267113 22-23 21.302993503883584 26.88584045867872 31.848404483728793 19.9627615537089 24-25 20.55098829709205 27.09550418010076 32.32840286998658 20.025104652820602 26-27 20.483753988664485 27.288369438682025 31.939760914158317 20.288115658495173 28-29 20.14684389976375 27.358265702767365 32.242182490651544 20.252707906817342 30-31 20.310235130862328 27.132453144118852 32.344870623695705 20.21244110132312 32-33 20.05043155876552 27.37176932221909 32.00806252104536 20.569736597970024 34-35 20.458447971811605 27.386320003916964 31.96409965236946 20.19113237190197 36-37 20.82702663477141 26.976610575158027 31.72199600407579 20.47436678599477 38-39 20.450798415876953 27.24643555140328 31.844883371860178 20.45788266085959 40-41 20.455049968333707 27.366416556639724 31.828293562618747 20.35023991240782 42-43 20.570791795404723 27.41685793974737 31.42279259413534 20.589557670712566 44-45 20.33997879373363 27.890921287303684 31.03368309214677 20.735416826815918 46-47 20.909482099635998 27.95389073743556 30.45290729533668 20.68371986759176 48-49 20.89880286734657 28.365335889415107 29.714622402476376 21.021238840761946 50-51 21.112381371210944 28.52931652467144 29.390521378305696 20.967780725811924 52-53 21.15553963299647 28.79046399081761 29.240176053101163 20.813820323084762 54-55 20.875989953499076 28.84560139232239 29.16637144656989 21.11203720760865 56-57 21.022907062663933 29.0901620234708 28.976041032035027 20.910889881830247 58-59 21.074381407202818 28.54446556355907 29.12563001343428 21.255523015803842 60-61 20.85831625350219 28.680378043136486 29.587705612688676 20.873600090672653 62-63 21.031871259535382 28.70594468481024 29.68190567213189 20.580278383522487 64-65 20.942840190043253 28.696728511725716 29.580611672736108 20.779819625494923 66-67 20.78470837043442 28.95842474439963 29.294416762335235 20.962450122830713 68-69 20.81019343480968 28.960647502093217 29.334678717877154 20.89448034521995 70-71 20.747003232588824 29.07028615860337 29.24945461602435 20.933255992783455 72-73 20.82886289703163 29.21258926382594 28.817473534802428 21.141074304340005 74-75 20.66539836615072 29.523494258084988 28.80501961915778 21.00608775660652 76-77 20.768387887364824 29.42615862732857 28.908126471121154 20.89732701418545 78-79 20.839063792842115 29.290330448029973 28.622169246035412 21.248436513092496 80-81 20.60382436120751 29.58987532206356 28.632733121207526 21.1735671955214 82-83 20.784549107093024 29.396977589095346 28.79070074972071 21.02777255409092 84-85 20.72279649716612 29.322839256536476 28.58682856841113 21.36753567788628 86-87 20.95006111049817 29.11070594967689 28.560647071525686 21.378585868299258 88-89 20.96203333771242 29.01555605356908 28.575114607509526 21.447296001208972 90-91 20.622054374049252 29.067115941039606 28.87721336539737 21.433616319513767 92-93 20.83033882776806 29.22066436379529 28.624167441588895 21.32482936684775 94-95 20.42502657596777 29.04508554971466 28.686803722524573 21.843084151792997 96-97 20.83438519121488 28.984609387209066 28.37354435749086 21.807461064085192 98-99 20.77633353970758 29.14642639094381 28.240240229925522 21.83699983942309 100-101 20.929214328149094 28.638355374086117 28.900629457800914 21.531800839963875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2346.0 1 1375.5 2 534.5 3 694.5 4 675.5 5 750.5 6 1273.5 7 2467.5 8 3803.0 9 4322.0 10 3850.5 11 3237.0 12 3195.0 13 3383.0 14 3482.5 15 3503.0 16 3628.0 17 3823.5 18 3810.5 19 3960.0 20 4284.0 21 4776.0 22 5495.5 23 6233.5 24 7019.0 25 8115.5 26 9685.5 27 11310.5 28 12436.5 29 13920.5 30 16636.5 31 19233.5 32 22054.0 33 24974.0 34 27585.5 35 29552.0 36 32570.0 37 35733.5 38 36110.5 39 35841.5 40 36774.5 41 38295.5 42 38962.5 43 40362.0 44 42794.0 45 44893.0 46 43696.0 47 41588.0 48 42400.5 49 40663.5 50 36979.5 51 33617.5 52 30735.0 53 29579.5 54 28435.0 55 26656.5 56 24327.0 57 21831.5 58 20062.0 59 18275.0 60 15753.5 61 13275.5 62 10728.0 63 7937.5 64 5567.5 65 4117.0 66 3095.0 67 2280.5 68 1580.5 69 1051.0 70 792.5 71 619.0 72 495.5 73 429.0 74 349.5 75 278.0 76 226.0 77 152.0 78 82.0 79 62.5 80 45.0 81 34.5 82 34.5 83 20.5 84 13.0 85 10.5 86 11.0 87 8.5 88 4.0 89 2.0 90 1.0 91 1.5 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30441361160046465 2 0.27080895495518664 3 0.2718376689341237 4 0.2734664660674408 5 0.28178190406384884 6 0.19099789542265144 7 0.193141049545437 8 0.19408403735946267 9 0.1920266094015885 10-11 0.20094213055237656 12-13 0.19022635993844864 14-15 0.19061212768055003 16-17 0.19657009614189397 18-19 0.1894119613717901 20-21 0.1821681004367748 22-23 0.18148229111748343 24-25 0.18679731334199168 26-27 0.18101079721047061 28-29 0.19506988825594404 30-31 0.19918474417169235 32-33 0.20171366603657934 34-35 0.20042777356290797 36-37 0.17732457211927938 38-39 0.16708029541236427 40-41 0.1729525377087968 42-43 0.18495420079639607 44-45 0.19086930617528428 46-47 0.19091216925774002 48-49 0.1905692645980943 50-51 0.19408403735946267 52-53 0.21757300654519268 54-55 0.181696606529762 56-57 0.16729461082464284 58-59 0.16686598000008573 60-61 0.16082228537383036 62-63 0.16806614630884567 64-65 0.1652800459492244 66-67 0.162365356342236 68-69 0.1740241147701896 70-71 0.1678946939790228 72-73 0.16369411189836305 74-75 0.16755178931937711 76-77 0.17162378215266974 78-79 0.17492423950175953 80-81 0.16669452767026288 82-83 0.16613730759833864 84-85 0.15842195275631052 86-87 0.15529294773704358 88-89 0.16099373770365322 90-91 0.16806614630884567 92-93 0.1604793807141847 94-95 0.1645085104650216 96-97 0.1627939871667931 98-99 0.1675089262369214 100-101 0.14513439719503987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1166505.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.53046659491266 #Duplication Level Percentage of deduplicated Percentage of total 1 75.46484533258841 38.132738461686095 2 13.31983180441724 13.461146320859212 3 4.5005919153365035 6.8225102830573565 4 2.077274023469089 4.198625026055384 5 1.1599855505360952 2.930730555597277 6 0.7107614477952419 2.154906455686154 7 0.4810445088678691 1.7015182440209817 8 0.34968869656533114 1.4135946400330412 9 0.2336994296217535 1.0628047099576943 >10 1.5047973123415164 14.184849507251737 >50 0.12479007732456605 4.348440073798591 >100 0.06910659780683363 5.970264814042501 >500 0.002218235394528262 0.7609082641252063 >1k 8.531674594339469E-4 0.6751174740057072 >5k 3.412669837735788E-4 1.225213193565913 >10k+ 1.706334918867894E-4 0.9566319762571291 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11095 0.951131799692243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8362 0.7168421909893228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5848 0.5013266124020043 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2000 0.17145232982284686 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1701 0.14582020651433125 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1520 0.13030377066536364 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1454 0.12464584378120969 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.572616491142344E-5 0.0 0.0 0.0 0.0 3 8.572616491142344E-5 0.0 0.0 0.0 0.0 4 8.572616491142344E-5 0.0 0.0 0.0 0.0 5 8.572616491142344E-5 0.0 0.0 0.0 0.0 6 8.572616491142344E-5 0.0 0.0 0.0 0.0 7 1.7145232982284687E-4 0.0 0.0 0.0 0.0 8 1.7145232982284687E-4 0.0 0.0 0.0 0.0 9 1.7145232982284687E-4 0.0 0.0 0.0 0.0 10-11 2.571784947342703E-4 0.0 0.0 0.0 0.0 12-13 2.571784947342703E-4 0.0 0.0 0.0 0.0 14-15 3.4290465964569375E-4 0.0 0.0 0.0 0.0 16-17 3.4290465964569375E-4 0.0 0.0 8.572616491142344E-5 0.0 18-19 4.286308245571172E-4 0.0 0.0 1.7145232982284687E-4 0.0 20-21 5.143569894685406E-4 0.0 0.0 2.143154122785586E-4 0.0 22-23 5.143569894685406E-4 0.0 0.0 3.8576774210140547E-4 0.0 24-25 5.143569894685406E-4 0.0 0.0 8.143985666585227E-4 0.0 26-27 5.143569894685406E-4 0.0 0.0 0.0010287139789370812 0.0 28-29 6.858093192913875E-4 0.0 0.0 0.0015430709684056219 0.0 30-31 6.858093192913875E-4 0.0 0.0 0.003043278854355532 0.0 32-33 6.858093192913875E-4 0.0 0.0 0.005529337636786811 0.0 34-35 8.143985666585227E-4 0.0 0.0 0.007415313264838128 0.0 36-37 9.429878140256578E-4 0.0 0.0 0.011315853768307894 0.0 38-39 0.0010287139789370812 0.0 0.0 0.01817394696122177 0.0 40-41 0.0010287139789370812 0.0 0.0 0.024774861659401376 0.0 42-43 0.001071577061392793 0.0 0.0 0.035576358438240724 0.0 44-45 0.0012001663087599281 0.0 0.0 0.04393465951710451 0.0 46-47 0.0012858924736713516 0.0 0.0 0.059622547695895 0.0 48-49 0.0012858924736713516 0.0 0.0 0.07822512548167389 0.0 50-51 0.0012858924736713516 0.0 0.0 0.08996961007453891 0.0 52-53 0.0012858924736713516 0.0 0.0 0.11332999001290178 0.0 54-55 0.0012858924736713516 0.0 0.0 0.14984933626516816 0.0 56-57 0.0014573448034941984 0.0 0.0 0.20471408180847916 0.0 58-59 0.0014573448034941984 0.0 0.0 0.2607789936605501 0.0 60-61 0.0014573448034941984 0.0 0.0 0.3405043270281739 0.0 62-63 0.0014573448034941984 0.0 0.0 0.41568617365549226 0.0 64-65 0.0014573448034941984 0.0 0.0 0.4897535801389621 0.0 66-67 0.0014573448034941984 0.0 0.0 0.5968684231957857 0.0 68-69 0.0014573448034941984 0.0 0.0 0.7401168447627742 0.0 70-71 0.0014573448034941984 0.0 0.0 0.8336869537635929 0.0 72-73 0.0015002078859499102 0.0 0.0 0.9482171100852547 0.0 74-75 0.0015430709684056219 0.0 0.0 1.0828071889961894 0.0 76-77 0.0015430709684056219 0.0 0.0 1.2198833266895557 0.0 78-79 0.0015430709684056219 0.0 0.0 1.3722615848196107 0.0 80-81 0.0017145232982284687 0.0 0.0 1.6085657583979494 0.0 82-83 0.0018002494631398922 0.0 0.0 1.8786889040338446 0.0 84-85 0.0018859756280513156 0.0 0.0 2.1997333916271256 0.0 86-87 0.0020145648754184508 0.0 0.0 2.5870870677793922 0.0 88-89 0.0020574279578741625 0.0 0.0 3.000158593405086 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5515 0.0 49.243107 1 GTATCAA 9465 0.0 39.345585 1 TATCAAC 12075 0.0 30.674284 2 ATCAACG 12180 0.0 30.40985 3 TCAACGC 12295 0.0 30.124119 4 CAACGCA 12500 0.0 29.630083 5 AACGCAG 12845 0.0 28.879726 6 GTATAGA 270 0.0 28.18455 1 GTACATG 10325 0.0 28.05318 1 TACATGG 10555 0.0 27.343527 2 ACATGGG 10580 0.0 26.155405 3 ACGCAGA 14595 0.0 25.223831 7 CGCAGAG 14575 0.0 25.223684 8 GAGTACT 7720 0.0 25.0114 12-13 CATGGGA 7645 0.0 24.130133 4 ATGGGAG 2750 0.0 24.031868 5 GGACCGA 200 2.0549123E-8 23.749044 6 GCAGAGT 15055 0.0 23.725382 9 GTACTTT 8300 0.0 23.09094 14-15 ATGGGAT 2810 0.0 22.503536 5 >>END_MODULE