FastQCFastQC Report
Thu 26 May 2016
SRR937579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166505
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165771.4210826357366664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140561.2049669739949678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100050.8576902799387914No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.2731235614077951No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29610.2538351743027248No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.11847355990758719No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13360.1145301563216617No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.1104153004059134No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA12420.10647189681998791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.051.1804281
GTATCAA103350.040.8799631
TATCACG756.909928E-638.0099642
ATCAACG138650.030.3270993
TATCAAC138850.030.2834152
GTAAGGT3300.030.2351974
TCAACGC141600.029.7623924
CAACGCA144250.029.2156335
AACGCAG147950.028.4764466
CATATAG3150.027.1499733
TAAGGTG4200.026.0187265
AAGGGTA3300.025.9158844
ACGCGAA851.124281E-725.14610778-79
ACGCAGA169250.024.8657077
GTACATG92550.024.8021851
CGCAGAG169550.024.6816318
GTATAGG3950.024.0631281
TACATGG95350.023.9181732
TAACGCA1000.001651138423.7562274
GCAGAGT176300.023.4941629
Produced by FastQC (version 0.11.5)