##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937579_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1166505 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18834724240359 33.0 31.0 34.0 31.0 34.0 2 32.46042408733782 34.0 31.0 34.0 31.0 34.0 3 32.516296972580484 34.0 31.0 34.0 31.0 34.0 4 36.16238764514511 37.0 35.0 37.0 35.0 37.0 5 36.09727605110994 37.0 35.0 37.0 35.0 37.0 6 36.1585316822474 37.0 36.0 37.0 35.0 37.0 7 36.103803241306295 37.0 35.0 37.0 35.0 37.0 8 36.09428163616958 37.0 35.0 37.0 35.0 37.0 9 37.77039446894784 39.0 37.0 39.0 35.0 39.0 10-11 37.70611227555818 39.0 37.0 39.0 35.0 39.0 12-13 37.59425377516599 39.0 37.0 39.0 35.0 39.0 14-15 38.73568651656015 40.0 38.0 41.0 34.5 41.0 16-17 38.70968962841994 40.0 38.0 41.0 34.5 41.0 18-19 38.80190054907608 40.0 38.0 41.0 35.0 41.0 20-21 38.76813987081067 40.0 38.0 41.0 35.0 41.0 22-23 38.703623644990806 40.0 38.0 41.0 34.5 41.0 24-25 38.703184298395634 40.0 38.0 41.0 35.0 41.0 26-27 38.547006228005884 40.0 38.0 41.0 34.0 41.0 28-29 38.380826914586734 40.0 38.0 41.0 34.0 41.0 30-31 38.28376346436578 40.0 37.5 41.0 34.0 41.0 32-33 38.07734900407628 40.0 37.0 41.0 34.0 41.0 34-35 37.885343397585096 40.0 36.0 41.0 33.5 41.0 36-37 37.694972160427945 40.0 36.0 41.0 33.0 41.0 38-39 37.52494331357345 40.0 36.0 41.0 33.0 41.0 40-41 37.28646726760708 40.0 36.0 41.0 33.0 41.0 42-43 36.96057282223394 40.0 35.5 41.0 32.5 41.0 44-45 36.47789422248512 40.0 35.0 41.0 31.0 41.0 46-47 35.83439976682483 39.0 35.0 41.0 29.0 41.0 48-49 35.263014303410614 39.0 35.0 41.0 25.5 41.0 50-51 34.929968581360555 39.0 35.0 41.0 24.0 41.0 52-53 34.681558587404254 39.0 35.0 40.0 23.0 41.0 54-55 34.44682148812049 38.5 35.0 40.0 22.5 41.0 56-57 34.18102237024273 38.0 34.0 40.0 21.5 41.0 58-59 33.921196651536 38.0 34.0 40.0 20.0 41.0 60-61 33.59217534429771 38.0 34.0 40.0 18.5 41.0 62-63 33.23936631218898 37.0 33.0 40.0 17.0 41.0 64-65 32.82737407897952 36.5 33.0 39.5 12.5 41.0 66-67 32.40649547151534 36.0 32.0 39.0 8.0 40.5 68-69 31.95831265189605 35.5 32.0 39.0 7.0 40.0 70-71 31.566474640057265 35.0 31.0 38.0 3.5 40.0 72-73 31.09979982940493 35.0 31.0 37.5 2.0 39.5 74-75 30.622475257285654 35.0 30.5 37.0 2.0 39.0 76-77 29.399713245978372 33.5 29.0 35.5 2.0 38.0 78-79 29.965265901132014 34.0 30.0 36.0 2.0 38.5 80-81 29.7711111396865 34.0 30.0 36.0 2.0 37.5 82-83 29.39294473662779 34.0 30.0 35.0 2.0 37.0 84-85 29.023488969185728 34.0 29.0 35.0 2.0 36.5 86-87 28.659137337602495 34.0 29.0 35.0 2.0 36.0 88-89 28.231839983540574 34.0 29.0 35.0 2.0 36.0 90-91 27.70057736572068 34.0 27.0 35.0 2.0 35.0 92-93 27.272983399128165 33.0 26.5 35.0 2.0 35.0 94-95 26.832947136960406 33.0 25.5 35.0 2.0 35.0 96-97 26.29702701660087 33.0 25.0 35.0 2.0 35.0 98-99 25.44625355227796 32.5 22.0 34.0 2.0 35.0 100-101 22.780447576307004 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23292277509012393 1101 2 0.12480149103630112 1101 3 0.10899162975527332 1101 4 -0.02520046101991369 1101 5 0.10808692935911068 1101 6 0.12335836854020954 1101 7 0.16069427821253157 1101 8 0.08027911047622638 1101 9 0.18784805375518232 1101 10-11 0.1841759781481116 1101 12-13 0.1699276249706756 1101 14-15 0.2806276448484013 1101 16-17 0.1883690932248996 1101 18-19 0.14757629759468216 1101 20-21 0.2673197605187454 1101 22-23 0.07098609865274597 1101 24-25 0.011995752517549363 1101 26-27 -0.06429313594176733 1101 28-29 -0.2330564411314313 1101 30-31 -0.02009590856176402 1101 32-33 0.0858613686584917 1101 34-35 -0.0019993293475479845 1101 36-37 -0.2591406497570432 1101 38-39 -0.36813653895986675 1101 40-41 -0.2960365531909801 1101 42-43 -0.07167285485599706 1101 44-45 -0.13128577343359638 1101 46-47 -0.31049914412382407 1101 48-49 -0.3034527151638997 1101 50-51 -0.34809971273204354 1101 52-53 -0.2148949372218496 1101 54-55 -0.42510455108123324 1101 56-57 -0.4242684719078369 1101 58-59 -0.40967736608735805 1101 60-61 -0.3616858719497458 1101 62-63 -0.6465349892300409 1101 64-65 -0.4927932566811961 1101 66-67 -0.3886451961358688 1101 68-69 -0.41949511056041544 1101 70-71 -0.5692489273646721 1101 72-73 -0.6366139218639439 1101 74-75 -0.5176597628371447 1101 76-77 -0.5845738026923435 1101 78-79 -0.42555570552293887 1101 80-81 -0.5385110146053051 1101 82-83 -0.663545749301246 1101 84-85 -0.6514015179932535 1101 86-87 -0.9427999664544835 1101 88-89 -0.9384111584259607 1101 90-91 -0.8900708080469251 1101 92-93 -0.8320437038358435 1101 94-95 -1.0952495522486814 1101 96-97 -1.1380954206242038 1101 98-99 -1.3739982474644528 1101 100-101 -1.0157762650281121 1102 1 0.37786629416872586 1102 2 0.26218204999333494 1102 3 0.19580676477344383 1102 4 0.07643937905326226 1102 5 0.025813381708097438 1102 6 0.08321068703654788 1102 7 0.17203472268320752 1102 8 0.11840065879606243 1102 9 0.29343012444362415 1102 10-11 0.2617917554308278 1102 12-13 0.2759117257265373 1102 14-15 0.25353057241413524 1102 16-17 0.3654482771653349 1102 18-19 0.2818964487678173 1102 20-21 0.3661377060590354 1102 22-23 0.2905357020322654 1102 24-25 0.27820942842475915 1102 26-27 0.31616070313801714 1102 28-29 0.3041233486818342 1102 30-31 0.2634948100762031 1102 32-33 0.2761220980664305 1102 34-35 0.2587654252788667 1102 36-37 0.12488719973863738 1102 38-39 0.23401075649658054 1102 40-41 0.5041022516855023 1102 42-43 0.44754774777109674 1102 44-45 0.5848742369921354 1102 46-47 0.35361272596759363 1102 48-49 0.10253710253329018 1102 50-51 0.28732417695188417 1102 52-53 0.4541245888990346 1102 54-55 0.600193529010042 1102 56-57 0.5940669269010712 1102 58-59 0.5100306518921158 1102 60-61 0.4685882830494421 1102 62-63 0.5273669892004946 1102 64-65 0.3235948781451867 1102 66-67 0.3585565762388825 1102 68-69 0.43134036201547943 1102 70-71 0.4543884734802077 1102 72-73 0.2949779573745843 1102 74-75 0.33230372250535467 1102 76-77 0.39529180615575754 1102 78-79 0.4251377656071256 1102 80-81 0.6177732563064033 1102 82-83 0.6480217130652974 1102 84-85 0.630421866632723 1102 86-87 0.33820368255236843 1102 88-89 0.5213796875830035 1102 90-91 0.47271276453732725 1102 92-93 0.5237648929685719 1102 94-95 0.2372578982167859 1102 96-97 0.15665624282130608 1102 98-99 0.27286444266995247 1102 100-101 0.06484029172574779 1103 1 0.2306902026526103 1103 2 0.11158752961195262 1103 3 0.1819683973107047 1103 4 0.06661852043107075 1103 5 0.09206174785362009 1103 6 0.1453250213864976 1103 7 0.046063275873400755 1103 8 0.09395870242631332 1103 9 0.13771366025670062 1103 10-11 0.12227198199197886 1103 12-13 0.1394291484414225 1103 14-15 0.1728409591690223 1103 16-17 0.13367462047235534 1103 18-19 0.1816493441099496 1103 20-21 0.11036158718535916 1103 22-23 -0.019188645185884923 1103 24-25 -0.06991873162711926 1103 26-27 0.026427642029325682 1103 28-29 -0.05822251171353088 1103 30-31 -0.08720192120158998 1103 32-33 -0.04374924539257563 1103 34-35 0.06530882248029002 1103 36-37 0.0951421823615135 1103 38-39 0.35827678853532774 1103 40-41 0.24507552233286845 1103 42-43 0.21721859575423963 1103 44-45 0.2491931349889498 1103 46-47 0.3395362463793816 1103 48-49 0.3249534593164469 1103 50-51 0.3014513186944612 1103 52-53 0.24615581198477088 1103 54-55 0.15657227420980036 1103 56-57 0.21763065482503663 1103 58-59 0.10022131891003738 1103 60-61 0.2843648578825082 1103 62-63 0.270872101011868 1103 64-65 0.2352559128690217 1103 66-67 0.280534325420696 1103 68-69 0.2227915034338146 1103 70-71 0.15902304736082584 1103 72-73 0.12048419895200269 1103 74-75 5.666692189016942E-4 1103 76-77 -0.06987479633884774 1103 78-79 0.12701695156762582 1103 80-81 0.14477175164041967 1103 82-83 -0.05999139323403213 1103 84-85 0.09215230850803025 1103 86-87 0.014377524668830688 1103 88-89 0.17378158032108715 1103 90-91 0.22592221201587392 1103 92-93 0.02265729161893404 1103 94-95 -0.333030141461812 1103 96-97 -0.2545614980519062 1103 98-99 -0.07487551862416453 1103 100-101 -0.4051518809714061 1104 1 0.33028129907228987 1104 2 0.22958867797633786 1104 3 0.22374685957566953 1104 4 0.10375120481331379 1104 5 0.09191954385259038 1104 6 0.11564060694501421 1104 7 0.13850660892053668 1104 8 0.1438736483351306 1104 9 0.25046265141322266 1104 10-11 0.19603292901696534 1104 12-13 0.2165607327549779 1104 14-15 0.2105733151405147 1104 16-17 0.2456991761519305 1104 18-19 0.14278338077174624 1104 20-21 0.10067360422837623 1104 22-23 0.1566224542388639 1104 24-25 0.20781775630968724 1104 26-27 0.17256036397488828 1104 28-29 0.12779172478513345 1104 30-31 0.21034988935048204 1104 32-33 0.3317290161769293 1104 34-35 0.3867126711108355 1104 36-37 0.38806297204984475 1104 38-39 0.19515001769304519 1104 40-41 0.1114238845720692 1104 42-43 0.27444430301790845 1104 44-45 0.42850164791073553 1104 46-47 0.290893087196757 1104 48-49 0.36181045575329307 1104 50-51 0.26709697852102465 1104 52-53 0.4334351274737358 1104 54-55 0.4876439825896455 1104 56-57 0.5186771957797873 1104 58-59 0.5692718254782534 1104 60-61 0.568084646240024 1104 62-63 0.517812702668941 1104 64-65 0.19642147588955083 1104 66-67 0.28837432713787337 1104 68-69 0.3278675154970081 1104 70-71 0.4955400957423812 1104 72-73 0.40651614387343926 1104 74-75 0.22481097569267305 1104 76-77 0.374385259898812 1104 78-79 0.3611028481504377 1104 80-81 0.3194552806607689 1104 82-83 0.14470925392832612 1104 84-85 0.29555702109496806 1104 86-87 0.23238631985213942 1104 88-89 0.29535862269251467 1104 90-91 0.31930529886227177 1104 92-93 0.517998098264421 1104 94-95 0.3120474536270521 1104 96-97 0.6548787011410155 1104 98-99 0.6440616272073818 1104 100-101 0.3079012389307145 1105 1 0.22766761833293003 1105 2 0.10840221661477045 1105 3 0.034271277070352824 1105 4 0.03677461892360867 1105 5 0.014877249844694518 1105 6 0.06434800378625027 1105 7 0.06653682977357533 1105 8 0.07351809772116269 1105 9 0.06173147165013404 1105 10-11 0.13270174708438986 1105 12-13 0.12086328569029092 1105 14-15 0.06488325786624927 1105 16-17 0.03284066962179821 1105 18-19 0.07419731208602087 1105 20-21 -0.12802651129642584 1105 22-23 -0.1495908001977213 1105 24-25 0.012188308876183385 1105 26-27 -0.027647702693222698 1105 28-29 -0.04243413161699294 1105 30-31 0.09653254056321714 1105 32-33 -0.023876173761024688 1105 34-35 0.035321356623050804 1105 36-37 0.19324538461981433 1105 38-39 0.16267019385480808 1105 40-41 0.12675193703008603 1105 42-43 0.0016888332170665876 1105 44-45 -0.03963547405741252 1105 46-47 -0.048547105669996427 1105 48-49 -0.025076598418614537 1105 50-51 0.103794813380226 1105 52-53 0.2983826396228224 1105 54-55 0.24252293917835743 1105 56-57 0.3096705327650824 1105 58-59 0.25630131955557545 1105 60-61 0.22670141306434743 1105 62-63 -0.15764092198936197 1105 64-65 -0.15397696262739657 1105 66-67 0.020842218837444193 1105 68-69 0.15325659813249004 1105 70-71 0.20000915624917326 1105 72-73 0.05276721505407167 1105 74-75 0.13402536639427964 1105 76-77 0.0896769735708709 1105 78-79 0.24766244647126 1105 80-81 0.14196031114196117 1105 82-83 0.07545030753616899 1105 84-85 0.28843604276806545 1105 86-87 0.05401338899345731 1105 88-89 0.16717728401502896 1105 90-91 0.28838716146911736 1105 92-93 0.22561256544019415 1105 94-95 0.11411632376199421 1105 96-97 0.09745257192680157 1105 98-99 -0.01069922832696335 1105 100-101 0.1312408411566004 1106 1 0.09763154224656034 1106 2 0.14270278328764618 1106 3 0.1366570656718764 1106 4 0.10105537127934383 1106 5 0.12344341586106111 1106 6 0.07178421820326264 1106 7 0.04859533141295458 1106 8 0.04506980690401008 1106 9 -0.057101633269205365 1106 10-11 -0.1243235861272538 1106 12-13 -0.11584652695995601 1106 14-15 0.06592224613680742 1106 16-17 0.051195877649497845 1106 18-19 0.13364252629107654 1106 20-21 0.28187868620940293 1106 22-23 0.4055668431732258 1106 24-25 0.23550365822137564 1106 26-27 0.24534373121618813 1106 28-29 0.27477935841060486 1106 30-31 0.2643496997467807 1106 32-33 0.12467237282449162 1106 34-35 -0.15147968334574813 1106 36-37 0.10868229898681392 1106 38-39 0.23282698114468303 1106 40-41 0.20228393296616076 1106 42-43 -0.24151889881672872 1106 44-45 -0.22004683044434614 1106 46-47 -0.09142997944744025 1106 48-49 -0.26495428819142575 1106 50-51 -0.07188433799074545 1106 52-53 0.043164882012185046 1106 54-55 0.10923149222030304 1106 56-57 0.05936487033557114 1106 58-59 -0.0011418717336724171 1106 60-61 0.0973505789698379 1106 62-63 -0.16593397174238333 1106 64-65 -0.28835944155159154 1106 66-67 -0.37326733993007366 1106 68-69 0.0914313276749823 1106 70-71 0.01965700899417655 1106 72-73 0.04055504874655469 1106 74-75 -0.07303047649108052 1106 76-77 0.05334481712903383 1106 78-79 -0.08558653584821485 1106 80-81 -0.2879164659805369 1106 82-83 -0.4174638301714495 1106 84-85 -0.5339467438927556 1106 86-87 -0.11484238133243352 1106 88-89 -0.23468942529227022 1106 90-91 -0.33636874228474056 1106 92-93 -0.3467866262985879 1106 94-95 -0.1048865642859802 1106 96-97 0.02955968539487941 1106 98-99 -0.40097188449387744 1106 100-101 -0.35298765631173623 1107 1 0.16594479291327957 1107 2 0.09541384594945868 1107 3 0.024260577380822212 1107 4 0.04107133359271131 1107 5 0.059140988421461316 1107 6 -0.030156565507397204 1107 7 -0.0022800652116004017 1107 8 0.060471492680029826 1107 9 0.031691654772686206 1107 10-11 0.03911103651976333 1107 12-13 -0.012750227491380883 1107 14-15 0.09990562197142339 1107 16-17 -0.005532626759595871 1107 18-19 0.027750486034904043 1107 20-21 0.06948414454192431 1107 22-23 0.003032728594966727 1107 24-25 -0.0233139626152834 1107 26-27 0.04477375983154985 1107 28-29 0.03691441515468341 1107 30-31 0.014753234815209737 1107 32-33 -0.04765053309518663 1107 34-35 -0.09633534120718679 1107 36-37 -0.12296201395238882 1107 38-39 0.03491765487110143 1107 40-41 0.2224338229819196 1107 42-43 0.2538917414277648 1107 44-45 0.018469453061911167 1107 46-47 0.21518270085856983 1107 48-49 0.21101503302093505 1107 50-51 0.22425823272035217 1107 52-53 0.48107615490934563 1107 54-55 0.2693163650084571 1107 56-57 0.35526489174843334 1107 58-59 0.5715520606406344 1107 60-61 0.4348896574382337 1107 62-63 0.6077885565323271 1107 64-65 0.6436936623397145 1107 66-67 0.7114454357604956 1107 68-69 0.6568250322943179 1107 70-71 0.6621669854400345 1107 72-73 0.605382675228423 1107 74-75 0.3060760232850548 1107 76-77 0.17129385452367174 1107 78-79 0.2732357568850432 1107 80-81 0.47027589936177705 1107 82-83 0.37479289054646614 1107 84-85 0.6094158536817496 1107 86-87 0.4813165410167528 1107 88-89 0.3189493981684386 1107 90-91 0.29330480894066113 1107 92-93 0.37325775671907024 1107 94-95 0.48957684892380726 1107 96-97 0.2870847481734913 1107 98-99 0.2272611652812877 1107 100-101 0.5273759729732674 1108 1 0.0055649352647435535 1108 2 0.058175616588520995 1108 3 0.005285203422765505 1108 4 0.03911951994371776 1108 5 0.03547182235743662 1108 6 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-0.6212190424579163 2316 54-55 -0.65567633161087 2316 56-57 -0.6789173935937711 2316 58-59 -0.8242855403308127 2316 60-61 -0.5870532178059449 2316 62-63 -0.6893217685121087 2316 64-65 -0.5703921831805872 2316 66-67 -0.5332730761097011 2316 68-69 -0.4437402317712902 2316 70-71 -0.4175707967960349 2316 72-73 -0.3955743316174001 2316 74-75 -0.5807750757417445 2316 76-77 -0.5048422171374511 2316 78-79 -0.3766204633074892 2316 80-81 -0.5672581977467885 2316 82-83 -0.6084139095168872 2316 84-85 -0.5898756304003996 2316 86-87 -0.7492967669747301 2316 88-89 -0.8303341665852706 2316 90-91 -0.3932742171579271 2316 92-93 -0.4005697427089814 2316 94-95 -0.4518437026134272 2316 96-97 -0.5691646578768754 2316 98-99 -0.7888235187375052 2316 100-101 -0.553974536503528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 42.0 6 53.0 7 150.0 8 339.0 9 646.0 10 1129.0 11 1983.0 12 3391.0 13 4861.0 14 6592.0 15 9992.0 16 14926.0 17 22963.0 18 20275.0 19 9814.0 20 6928.0 21 6634.0 22 6990.0 23 7244.0 24 8027.0 25 9288.0 26 10868.0 27 13138.0 28 16181.0 29 19955.0 30 24459.0 31 31123.0 32 39948.0 33 53067.0 34 73770.0 35 111153.0 36 165590.0 37 252499.0 38 196895.0 39 15576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33175227042006 19.9674355914067 15.891054133399182 19.809758004774057 2 15.623864127341184 21.329116745420503 40.628321891738054 22.418697235500264 3 19.200003429046596 27.011028671115856 31.15880343419017 22.630164465647383 4 12.238438755084633 17.84947342703203 37.34917552860896 32.56291228927437 5 12.18105774813242 39.32342607417495 33.195035336310255 15.300480841382374 6 29.7864989862881 37.442102691372945 17.05316308116982 15.718235241169134 7 27.04883390984179 35.8185348541155 19.808402021422967 17.32422921461974 8 27.809139266441207 34.01314182108092 18.856155781586878 19.321563130890993 9 27.890750575436883 16.074856087200658 20.51924338086849 35.51514995649397 10-11 24.80349419848179 28.221396393500243 25.668556928602964 21.306552479415007 12-13 26.651789771119848 26.04834105183024 27.57187975261946 19.727989424430454 14-15 23.176197273050693 26.051024213355277 27.91145344426299 22.861325069331038 16-17 19.959837291739 29.866352908903092 28.429453795740265 21.744356003617643 18-19 19.91135914548159 26.208717493709845 34.01914265262472 19.86078070818385 20-21 21.13411429869568 25.03615501005139 34.46234692521678 19.36738376603615 22-23 20.621698984223706 25.616782554948703 34.46159741516695 19.29992104566064 24-25 20.224362711143726 25.51075692839459 34.90206634525042 19.362814015211264 26-27 20.095431266684614 25.773385889683105 34.55195654671177 19.579226296920517 28-29 19.665990423435705 25.83764933109357 34.84580068384436 19.65055956162636 30-31 19.875611334713525 25.62732264328057 34.90932314906494 19.587742872940964 32-33 19.72923390812727 25.78793061324212 34.5596889854737 19.92314649315691 34-35 20.028889717575147 25.701004282021938 34.598222896601385 19.671883103801527 36-37 20.37003699084016 25.358399663953435 34.348545441296864 19.923017903909543 38-39 20.137719083930204 25.626551107796363 34.36401901406338 19.871710794210053 40-41 19.995387898720658 25.875988483463086 34.30927568149583 19.819347936320426 42-43 20.25674264520543 25.787392123678178 33.92674955546296 20.02911567565343 44-45 20.196270054564707 26.09898800262322 33.480525158486245 20.224216784325826 46-47 20.57020758590833 26.577511455158785 32.66629804415755 20.185982914775334 48-49 20.677836785954625 26.948234255318237 31.793519959194345 20.580408999532793 50-51 20.865277045533453 27.18736739233865 31.276205417036362 20.671150145091534 52-53 20.996266625518107 27.543045250556148 30.89489543551035 20.565792688415392 54-55 20.75624226053136 27.600793653174932 30.776435067331505 20.866529018962208 56-57 20.850575008251145 27.761432655668 30.610584609581615 20.777407726499245 58-59 21.057260791852585 27.254062348639742 30.803982837621785 20.884694021885892 60-61 20.787051920051777 27.155777300568793 31.38173432604232 20.675436453337106 62-63 21.030599954565133 27.18719594000883 31.545771342600332 20.236432762825707 64-65 20.8570044706195 27.278794347216685 31.274919524562687 20.589281657601123 66-67 20.883965349484143 27.56490542260856 30.933129305060845 20.61799992284645 68-69 20.94375934950986 27.531515081375563 30.971105996116606 20.553619572997974 70-71 20.7688351100081 27.73485754454546 30.863133891410666 20.633173454035774 72-73 20.90359664124886 27.80360992880442 30.339046982224684 20.95374644772204 74-75 20.784446180876284 28.066372654370387 30.229201859058406 20.91997930569492 76-77 20.71577061392793 27.883035220594856 30.73064410354006 20.670550061937153 78-79 20.81632740536903 27.890107629200045 30.3504485621579 20.943116403273024 80-81 20.65619092931449 28.135284460846716 30.342818933480782 20.865705676358008 82-83 20.7376799729103 28.039682981924486 30.295886394712362 20.926750650452853 84-85 20.53819766661852 27.858786657059614 30.323157776073735 21.279857900248132 86-87 20.73175854368391 27.60819713588883 30.1617224101054 21.498321910321856 88-89 20.708698205322737 27.607254148074805 30.188168931980574 21.49587871462188 90-91 20.383653980941617 27.602501278546526 30.500225701507294 21.513619039004563 92-93 20.454109021828025 27.57655068830895 30.433817238500037 21.535523051362986 94-95 20.109300860262064 27.4083694454803 30.50458420666864 21.977745487588997 96-97 20.564592522106633 27.238717365120596 30.205271301880405 21.991418810892366 98-99 20.403784174653953 27.50170490175865 30.033403644010516 22.061107279576884 100-101 20.181833147701813 27.277115706312927 30.566351907631812 21.97469923835344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 945.0 1 686.5 2 720.0 3 1054.0 4 808.5 5 630.5 6 1446.0 7 3629.5 8 5830.5 9 6633.0 10 5516.5 11 4022.0 12 3761.5 13 3857.5 14 3962.0 15 4113.5 16 4224.0 17 4292.0 18 4467.0 19 4662.5 20 4888.0 21 5252.5 22 5869.0 23 6571.5 24 7257.5 25 8428.5 26 9892.5 27 11331.5 28 12426.5 29 13882.5 30 16249.0 31 18504.0 32 21229.0 33 23743.5 34 26249.0 35 28738.0 36 31916.5 37 35086.0 38 35633.0 39 35277.5 40 36109.0 41 37662.0 42 38438.5 43 40096.0 44 42700.0 45 45069.0 46 43933.5 47 41551.0 48 42305.5 49 40421.0 50 36758.5 51 33396.5 52 30491.5 53 29449.0 54 28232.5 55 26380.5 56 23787.5 57 21382.5 58 20165.5 59 18423.0 60 15452.0 61 12491.0 62 10214.5 63 7755.0 64 5374.5 65 4014.5 66 2958.0 67 2172.0 68 1499.0 69 987.0 70 698.5 71 529.5 72 459.5 73 446.0 74 395.0 75 322.0 76 250.5 77 151.5 78 100.5 79 82.0 80 57.5 81 42.5 82 34.5 83 21.0 84 12.0 85 7.5 86 2.5 87 3.0 88 2.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01723095914719611 2 8.572616491142344E-5 3 0.0 4 0.0 5 0.03634789392244354 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003686225091191208 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.858093192913875E-4 24-25 8.572616491142344E-5 26-27 9.429878140256578E-4 28-29 9.858508964813695E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.286724017470992E-4 42-43 0.010544318284105082 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.286308245571172E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.286308245571172E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.572616491142345E-4 84-85 4.286308245571172E-5 86-87 0.0 88-89 0.0 90-91 0.012130252334966417 92-93 0.001971701792962739 94-95 0.0 96-97 0.0 98-99 0.0013287555561270633 100-101 0.018516851620867462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1166505.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.424731552980155 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3043138980472 35.238621398432606 2 13.552722052918883 12.854684103436643 3 4.806152925174835 6.8379153683696074 4 2.3050224995049837 4.372602930504127 5 1.2390571080441273 2.938097536390233 6 0.7833854180524801 2.2291105892194807 7 0.5099604642425826 1.6929316683536328 8 0.38006076290594054 1.4419423723708054 9 0.27749483943871245 1.1844106440946442 >10 1.618558625592201 14.36033127953476 >50 0.1407502492050963 4.634498032177789 >100 0.07684897189008832 6.421682297537713 >500 0.0038424478916356027 1.1844758884863646 >1k 0.001280815963878534 1.0823419602264048 >5k 0.0 0.0 >10k+ 5.489211273765147E-4 3.52635393086512 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16577 1.4210826357366664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14056 1.2049669739949678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10005 0.8576902799387914 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3186 0.2731235614077951 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2961 0.2538351743027248 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1382 0.11847355990758719 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1336 0.1145301563216617 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.1104153004059134 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1242 0.10647189681998791 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.572616491142344E-5 0.0 0.0 0.0 0.0 14-15 8.572616491142344E-5 0.0 0.0 0.0 0.0 16-17 1.7145232982284687E-4 0.0 0.0 8.572616491142344E-5 0.0 18-19 5.572200719242523E-4 0.0 0.0 1.7145232982284687E-4 0.0 20-21 8.572616491142344E-4 0.0 0.0 2.143154122785586E-4 0.0 22-23 9.429878140256578E-4 0.0 0.0 3.8576774210140547E-4 0.0 24-25 0.0010287139789370812 0.0 0.0 8.143985666585227E-4 0.0 26-27 0.0010287139789370812 0.0 0.0 0.0010287139789370812 0.0 28-29 0.001071577061392793 0.0 0.0 0.0016287971333170453 0.0 30-31 0.0011144401438485047 0.0 0.0 0.0030861419368112437 0.0 32-33 0.0011573032263042164 0.0 0.0 0.005700789966609658 0.0 34-35 0.0012430293912156398 0.0 0.0 0.007586765594660975 0.0 36-37 0.0012858924736713516 0.0 0.0 0.011401579933219318 0.0 38-39 0.0012858924736713516 0.0 0.0 0.01873116703314602 0.0 40-41 0.0012858924736713516 0.0 0.0 0.025503534061148473 0.0 42-43 0.0012858924736713516 0.0 0.0 0.03651934625226638 0.0 44-45 0.001371618638582775 0.0 0.0 0.04517768890832015 0.0 46-47 0.0018002494631398922 0.0 0.0 0.060651261674832084 0.0 48-49 0.0018002494631398922 0.0 0.0 0.07976819645007951 0.0 50-51 0.0018002494631398922 0.0 0.0 0.09164127029031166 0.0 52-53 0.001843112545595604 0.0 0.0 0.11517310255849739 0.0 54-55 0.0018859756280513156 0.0 0.0 0.15263543662478946 0.0 56-57 0.001971701792962739 0.0 0.0 0.2081859914873918 0.0 58-59 0.001971701792962739 0.0 0.0 0.26467953416401985 0.0 60-61 0.001971701792962739 0.0 0.0 0.34461918294392224 0.0 62-63 0.001971701792962739 0.0 0.0 0.41967244032387346 0.0 64-65 0.001971701792962739 0.0 0.0 0.49421134071435613 0.0 66-67 0.002143154122785586 0.0 0.0 0.6013690468536355 0.0 68-69 0.0022288802876970094 0.0 0.0 0.7451318254100925 0.0 70-71 0.0022288802876970094 0.0 0.0 0.8387876605758227 0.0 72-73 0.002314606452608433 0.0 0.0 0.9549466140308014 0.0 74-75 0.0024003326175198562 0.0 0.0 1.0917655732294334 0.0 76-77 0.0024003326175198562 0.0 0.0 1.2294417940771791 0.0 78-79 0.0024003326175198562 0.0 0.0 1.3828487661861715 0.0 80-81 0.0024003326175198562 0.0 0.0 1.6215961354644857 0.0 82-83 0.0024003326175198562 0.0 0.0 1.8954055061915722 0.0 84-85 0.002571784947342703 0.0 0.0 2.2206934389479684 0.0 86-87 0.00274323727716555 0.0 0.0 2.6157624699422635 0.0 88-89 0.00274323727716555 0.0 0.0 3.0364636242450738 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5915 0.0 51.180428 1 GTATCAA 10335 0.0 40.879963 1 TATCACG 75 6.909928E-6 38.009964 2 ATCAACG 13865 0.0 30.327099 3 TATCAAC 13885 0.0 30.283415 2 GTAAGGT 330 0.0 30.235197 4 TCAACGC 14160 0.0 29.762392 4 CAACGCA 14425 0.0 29.215633 5 AACGCAG 14795 0.0 28.476446 6 CATATAG 315 0.0 27.149973 3 TAAGGTG 420 0.0 26.018726 5 AAGGGTA 330 0.0 25.915884 4 ACGCGAA 85 1.124281E-7 25.146107 78-79 ACGCAGA 16925 0.0 24.865707 7 GTACATG 9255 0.0 24.802185 1 CGCAGAG 16955 0.0 24.681631 8 GTATAGG 395 0.0 24.063128 1 TACATGG 9535 0.0 23.918173 2 TAACGCA 100 0.0016511384 23.756227 4 GCAGAGT 17630 0.0 23.494162 9 >>END_MODULE