FastQCFastQC Report
Thu 26 May 2016
SRR937578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133979
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108680.9583951731028528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82020.723293817610379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.5046830673231162No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.17116719092681612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17090.1507082582658056No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14720.12980840033192856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.12972021527735522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.048.8195721
GTATCAA89050.042.768921
ATCAACG113750.033.1761443
TATCAAC114800.032.7898982
TCAACGC115450.032.6905144
CAACGCA116550.032.3397675
TATCACG902.402955E-531.6858632
AACGCAG119400.031.5496966
GTACACG1802.0918378E-1029.056941
CGCAGAG134850.027.617958
ACGCAGA135350.027.586127
GCAGAGT140850.026.036759
GTACATG98500.025.6323241
GAGTACT73000.024.95564812-13
TACATGG99650.024.9448662
GTAAGGT3050.024.935344
ACATGGG99150.023.680513
AGTACTT76300.023.40936712-13
CATGGGT8000.023.1723374
GTACTTT81050.022.3314-15
Produced by FastQC (version 0.11.5)