##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937578_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1133979 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82350114067368 33.0 31.0 34.0 30.0 34.0 2 31.925743774796533 33.0 31.0 34.0 30.0 34.0 3 31.946305883971398 33.0 31.0 34.0 30.0 34.0 4 35.55175624945436 37.0 35.0 37.0 33.0 37.0 5 35.551221848023644 37.0 35.0 37.0 33.0 37.0 6 35.659563360520785 37.0 35.0 37.0 33.0 37.0 7 35.60919558475069 37.0 35.0 37.0 33.0 37.0 8 35.603379780401575 37.0 35.0 37.0 33.0 37.0 9 37.20503113373352 39.0 37.0 39.0 34.0 39.0 10-11 37.10544727900604 39.0 37.0 39.0 33.0 39.0 12-13 36.98548562186778 39.0 37.0 39.0 33.0 39.0 14-15 38.08880984568497 40.0 38.0 41.0 33.0 41.0 16-17 38.05633261286144 40.0 38.0 41.0 33.0 41.0 18-19 38.08679128978579 40.0 38.0 41.0 33.5 41.0 20-21 37.947674075093104 40.0 38.0 41.0 33.0 41.0 22-23 37.82478114674081 40.0 38.0 41.0 33.0 41.0 24-25 37.68735091214211 40.0 37.0 41.0 32.5 41.0 26-27 37.7625410170735 40.0 37.0 41.0 33.0 41.0 28-29 37.69329282111926 40.0 37.0 41.0 33.0 41.0 30-31 37.42676451680322 39.5 37.0 41.0 32.5 41.0 32-33 37.13308359325878 39.0 36.0 41.0 32.0 41.0 34-35 36.854204090199204 39.0 35.0 41.0 31.0 41.0 36-37 36.83153259451895 39.0 35.0 41.0 31.0 41.0 38-39 36.74999669306045 39.0 35.0 41.0 31.0 41.0 40-41 36.524727530227636 39.0 35.0 41.0 30.5 41.0 42-43 36.21411992638312 39.0 35.0 41.0 30.0 41.0 44-45 35.862825502059565 39.0 35.0 41.0 29.0 41.0 46-47 35.33109166924608 39.0 35.0 40.5 26.5 41.0 48-49 34.86882869964964 39.0 35.0 40.0 24.0 41.0 50-51 33.93087394034634 38.0 34.0 39.5 22.5 40.5 52-53 34.00459532319381 38.0 34.0 39.5 23.0 40.5 54-55 34.29414124952931 38.0 34.0 40.0 22.0 41.0 56-57 34.05227257294888 38.0 34.0 40.0 20.0 41.0 58-59 33.82842715782215 38.0 34.0 40.0 19.5 41.0 60-61 33.5347885631039 38.0 33.5 40.0 18.0 41.0 62-63 33.19311204175739 37.0 33.0 40.0 15.0 41.0 64-65 32.82025593066538 36.5 33.0 40.0 11.5 41.0 66-67 32.41384452445769 36.0 32.0 39.0 8.0 41.0 68-69 31.94785088612752 35.5 31.5 39.0 6.5 40.0 70-71 31.518252101670313 35.0 31.0 38.5 2.0 40.0 72-73 31.067700548246485 35.0 31.0 38.0 2.0 40.0 74-75 30.59207622010637 35.0 30.5 37.0 2.0 39.0 76-77 30.145910991297015 35.0 30.0 36.5 2.0 39.0 78-79 29.632172200719765 34.0 29.0 36.0 2.0 38.5 80-81 29.113043980532268 34.0 29.0 35.5 2.0 37.5 82-83 28.619130512998918 34.0 29.0 35.0 2.0 37.0 84-85 28.16112555876255 34.0 27.5 35.0 2.0 36.5 86-87 27.654876324870212 34.0 26.5 35.0 2.0 36.0 88-89 27.192332485874957 33.0 26.0 35.0 2.0 36.0 90-91 26.706713263649505 33.0 25.0 35.0 2.0 35.0 92-93 26.170175109062868 33.0 24.5 35.0 2.0 35.0 94-95 25.562992789108087 32.0 21.5 35.0 2.0 35.0 96-97 24.828594268500563 32.0 17.5 34.0 2.0 35.0 98-99 23.95898204464104 31.5 2.0 34.0 2.0 35.0 100-101 21.516377287410087 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2921124580853025 1101 2 0.3655232298845732 1101 3 0.3084642286596697 1101 4 0.20720800619422874 1101 5 0.2832219279766761 1101 6 0.23670472920314722 1101 7 0.11257337027248582 1101 8 0.21483448894820611 1101 9 0.38020760174281776 1101 10-11 0.26929406384250143 1101 12-13 0.42387781235483857 1101 14-15 0.5423869110488582 1101 16-17 0.5780902059809563 1101 18-19 0.5766360583828316 1101 20-21 0.5908405552866114 1101 22-23 0.5438034844080661 1101 24-25 0.5990123328185106 1101 26-27 0.7196455233403114 1101 28-29 0.6449500299558011 1101 30-31 0.7116709040582023 1101 32-33 0.6917597953514942 1101 34-35 0.7741117377167157 1101 36-37 0.6719580170389676 1101 38-39 0.8167479271853608 1101 40-41 0.9125988630946722 1101 42-43 0.8259673317491831 1101 44-45 1.0319814023598752 1101 46-47 1.0911411648559408 1101 48-49 1.1514959046468007 1101 50-51 1.134446986774556 1101 52-53 1.2658275665129324 1101 54-55 1.4367465649652829 1101 56-57 1.3681885431409313 1101 58-59 1.2947218788437027 1101 60-61 1.2749267961392405 1101 62-63 1.4606957848168634 1101 64-65 1.3952605794560924 1101 66-67 1.5292045649564585 1101 68-69 1.6630659535823895 1101 70-71 1.6812002703697324 1101 72-73 1.536867373645073 1101 74-75 1.428191077044083 1101 76-77 1.4387294481666544 1101 78-79 1.5025883165732523 1101 80-81 1.5693735993035531 1101 82-83 1.7131552896869273 1101 84-85 1.7332103162310446 1101 86-87 1.7232286712902685 1101 88-89 1.6162895601827856 1101 90-91 1.7201395949274172 1101 92-93 1.9292117141004717 1101 94-95 2.0575275248236657 1101 96-97 2.031846140981262 1101 98-99 2.3254133226245592 1101 100-101 2.8224720355290494 1102 1 0.1737495974962684 1102 2 0.30443074393308933 1102 3 0.2915021162860647 1102 4 0.09869131575407408 1102 5 0.10368139167596979 1102 6 0.2280257626588238 1102 7 0.15853895984276534 1102 8 0.17810939110984947 1102 9 0.17554803353023374 1102 10-11 0.23998722798758365 1102 12-13 0.4091763453248376 1102 14-15 0.49972917753282076 1102 16-17 0.41489571464174446 1102 18-19 0.37068851449711815 1102 20-21 0.42603421135061836 1102 22-23 0.6491603619748005 1102 24-25 0.595074903658471 1102 26-27 0.6177754522692069 1102 28-29 0.5286587349987286 1102 30-31 0.38342281069852646 1102 32-33 0.37903000376513063 1102 34-35 0.5647724466409727 1102 36-37 0.7119252605372708 1102 38-39 0.7877893823926883 1102 40-41 0.7518091644067013 1102 42-43 0.777408908715536 1102 44-45 0.623247685811144 1102 46-47 0.6442320467465734 1102 48-49 0.6998107057442908 1102 50-51 0.6361915587843185 1102 52-53 0.6739343072046466 1102 54-55 0.6502141829868648 1102 56-57 0.7498877567978823 1102 58-59 0.7449126207945724 1102 60-61 0.7047340698903 1102 62-63 0.8568718034838056 1102 64-65 0.6824601946477102 1102 66-67 0.6872266353377512 1102 68-69 0.8237031937666046 1102 70-71 0.8670960147256501 1102 72-73 0.9359998406054686 1102 74-75 0.8586689604317677 1102 76-77 0.8672053721898543 1102 78-79 1.0041378351991668 1102 80-81 0.9630463895902572 1102 82-83 0.8584017530132506 1102 84-85 1.0219810072135012 1102 86-87 0.8252140602074967 1102 88-89 0.7948789839779486 1102 90-91 0.9878055617221122 1102 92-93 1.1488961068169594 1102 94-95 1.2046538768215207 1102 96-97 1.4048713228665939 1102 98-99 1.656897587971958 1102 100-101 1.5165158687773648 1103 1 0.24829061588989632 1103 2 0.23599372986939926 1103 3 0.220257947521727 1103 4 0.1750944635474454 1103 5 0.15341871759801506 1103 6 0.1239935115226487 1103 7 0.2105454203793684 1103 8 0.21280578025408659 1103 9 0.21348113433006688 1103 10-11 0.23678702867315593 1103 12-13 0.30769995807064987 1103 14-15 0.6197371887700314 1103 16-17 0.4040166478112752 1103 18-19 0.3363717463118263 1103 20-21 0.35418705201075085 1103 22-23 0.3984478343757658 1103 24-25 0.26992982079693206 1103 26-27 0.019093333007006663 1103 28-29 0.1834952241438188 1103 30-31 0.05615517953331306 1103 32-33 0.17935421187688405 1103 34-35 0.04532049688194206 1103 36-37 0.2073596070522754 1103 38-39 0.25690047870794785 1103 40-41 0.2771680592425696 1103 42-43 0.35306140573011646 1103 44-45 0.25321390115663434 1103 46-47 0.43194661044557137 1103 48-49 0.5170079110467469 1103 50-51 0.548948541232626 1103 52-53 0.4220898970293092 1103 54-55 0.4902171039741532 1103 56-57 0.8038465536947683 1103 58-59 0.7436529450241665 1103 60-61 0.7724043247893988 1103 62-63 0.7124160951014318 1103 64-65 0.8185083995291933 1103 66-67 1.132197167730638 1103 68-69 1.0556687819589428 1103 70-71 1.1692104014471916 1103 72-73 0.9127264447859176 1103 74-75 0.9693657877241009 1103 76-77 1.0946026221662315 1103 78-79 0.9587605123699277 1103 80-81 1.0555208739071347 1103 82-83 0.9204727330044911 1103 84-85 1.0204002376192598 1103 86-87 1.1154705271874512 1103 88-89 1.195491854363432 1103 90-91 1.309143159124151 1103 92-93 1.4166137203703393 1103 94-95 1.2111009897692249 1103 96-97 1.3528750643076712 1103 98-99 1.390993241714689 1103 100-101 1.601584791190792 1104 1 0.22566576250389048 1104 2 0.3188905848509478 1104 3 0.403957558608834 1104 4 0.2727037651662485 1104 5 0.29185615675617527 1104 6 0.45751956013913286 1104 7 0.3928073833685346 1104 8 0.42824108727683097 1104 9 0.49863940620819847 1104 10-11 0.46373361132071267 1104 12-13 0.4802592803460115 1104 14-15 0.5700685838703947 1104 16-17 0.5155741632324506 1104 18-19 0.6120722287727531 1104 20-21 0.6457301553362171 1104 22-23 0.6505808601560901 1104 24-25 0.4432539694860722 1104 26-27 0.5454446747327566 1104 28-29 0.5548022730119868 1104 30-31 0.6283634592466925 1104 32-33 0.6356955933486788 1104 34-35 0.8744763208406923 1104 36-37 0.9243693821096173 1104 38-39 0.9553549773237222 1104 40-41 1.0651274299539608 1104 42-43 1.2224995714288482 1104 44-45 1.1714297098247854 1104 46-47 1.3130625506508125 1104 48-49 1.3858581368441634 1104 50-51 1.049115930482671 1104 52-53 1.069853944667578 1104 54-55 1.1839030864896998 1104 56-57 1.262799385540987 1104 58-59 1.247054745705114 1104 60-61 1.3309775035706153 1104 62-63 1.4001318610319018 1104 64-65 1.174846278885468 1104 66-67 1.3293063197675679 1104 68-69 1.3899068284670406 1104 70-71 1.505903490289331 1104 72-73 1.4955461345103558 1104 74-75 1.3154697732502711 1104 76-77 1.3615347571033318 1104 78-79 1.3079196484989417 1104 80-81 1.464018608810047 1104 82-83 1.5953410772042744 1104 84-85 1.6638172101769193 1104 86-87 1.567209891440708 1104 88-89 1.7923440460941684 1104 90-91 1.9558705212120309 1104 92-93 1.906439481219774 1104 94-95 1.8248796774375045 1104 96-97 1.710449665303937 1104 98-99 1.892534172541378 1104 100-101 1.932479207750216 1105 1 0.16067545681285722 1105 2 0.22578382656084983 1105 3 0.24997605893767272 1105 4 0.3746526793445142 1105 5 0.2696239624521368 1105 6 0.2355024737142557 1105 7 0.11294112606823603 1105 8 0.1746135235082562 1105 9 0.3343473141493831 1105 10-11 0.30307450343365616 1105 12-13 0.2737872532400516 1105 14-15 0.34430452055219973 1105 16-17 0.3761784757709279 1105 18-19 0.316315648896051 1105 20-21 0.5649191710369976 1105 22-23 0.4976660301300768 1105 24-25 0.40928873759465745 1105 26-27 0.39504524029434407 1105 28-29 0.23552114330797735 1105 30-31 0.3077160594305255 1105 32-33 0.4971569866091059 1105 34-35 0.4472365281169104 1105 36-37 0.5864124996101552 1105 38-39 0.6951539427822908 1105 40-41 0.7909052906620815 1105 42-43 0.6251921649242362 1105 44-45 0.7308415993641972 1105 46-47 1.0616649079020277 1105 48-49 1.3183721461225772 1105 50-51 1.1158276248218684 1105 52-53 1.2021134249527492 1105 54-55 1.1621718688437497 1105 56-57 1.3311969895256723 1105 58-59 1.3987964690779577 1105 60-61 1.073962352065415 1105 62-63 1.1567081241336723 1105 64-65 1.1722352870962354 1105 66-67 1.4935343525160008 1105 68-69 1.4288217097404967 1105 70-71 1.2852007695097392 1105 72-73 1.4902817732342442 1105 74-75 1.451198311574494 1105 76-77 1.2559236214270264 1105 78-79 1.2211948400691313 1105 80-81 1.3234238624619223 1105 82-83 1.527991746344835 1105 84-85 1.5616429386298307 1105 86-87 1.5000326994460842 1105 88-89 1.2870284373697487 1105 90-91 1.4830618916673508 1105 92-93 1.2749342582506706 1105 94-95 1.389955067033089 1105 96-97 1.6015997565966664 1105 98-99 1.9555517799819846 1105 100-101 2.1706017903325225 1106 1 -0.024235699364044905 1106 2 0.01431983042953533 1106 3 0.1372739579816944 1106 4 0.03516319609084917 1106 5 0.001411559441940824 1106 6 0.08221089154594807 1106 7 0.025944359120408933 1106 8 0.09332193570867986 1106 9 0.23108462742882807 1106 10-11 -0.17350962525772218 1106 12-13 -0.27462265244323447 1106 14-15 -0.007620563864044527 1106 16-17 0.07731226134207247 1106 18-19 -0.023224163822028743 1106 20-21 0.12887524995641542 1106 22-23 -0.22990198679752183 1106 24-25 -0.09860806095867503 1106 26-27 -0.09393507067839835 1106 28-29 0.1943555129120753 1106 30-31 0.13300805831271134 1106 32-33 0.08646257374278576 1106 34-35 -0.030177508805913078 1106 36-37 0.1183847318239728 1106 38-39 0.06860173634560596 1106 40-41 -0.2604466009866755 1106 42-43 -0.04803762814775325 1106 44-45 -0.14581355370424376 1106 46-47 -0.1513117877961747 1106 48-49 0.04045284361152568 1106 50-51 0.19721318441863644 1106 52-53 0.34054408890045096 1106 54-55 0.33384039265592236 1106 56-57 0.32008086502113997 1106 58-59 0.12476942913325928 1106 60-61 -0.029783567904395625 1106 62-63 0.2989448218450548 1106 64-65 0.30260795742275803 1106 66-67 0.4974952461701889 1106 68-69 0.39652286393942404 1106 70-71 0.09497791908820119 1106 72-73 0.4135620717477977 1106 74-75 0.24858983750050356 1106 76-77 0.3253205618179358 1106 78-79 0.28252780361581387 1106 80-81 0.2051429339501709 1106 82-83 0.024533091471923285 1106 84-85 -0.07006750997815558 1106 86-87 0.06077543563733201 1106 88-89 0.453127284437862 1106 90-91 0.7276046643210634 1106 92-93 0.4663681834068818 1106 94-95 0.4058901497669858 1106 96-97 0.2698430611941056 1106 98-99 0.40908958937620454 1106 100-101 0.2274188282992391 1107 1 0.4327913538928492 1107 2 0.49296275774550935 1107 3 0.437653817105204 1107 4 0.450870566651858 1107 5 0.4924905738854406 1107 6 0.3799695354267456 1107 7 0.4311243026948972 1107 8 0.3504598308702853 1107 9 0.5164148460649116 1107 10-11 0.5446122599740804 1107 12-13 0.41598839122438136 1107 14-15 0.5368026070317029 1107 16-17 0.46011312219717126 1107 18-19 0.6225976412154068 1107 20-21 0.701133189795307 1107 22-23 0.7459579744163278 1107 24-25 0.6186054654995132 1107 26-27 0.674796955821499 1107 28-29 0.7595372783120382 1107 30-31 0.5335695891909538 1107 32-33 0.7007825475902294 1107 34-35 0.8168253329012884 1107 36-37 0.8099577674449208 1107 38-39 0.6054426228765308 1107 40-41 0.6633266038767474 1107 42-43 0.7489624893344526 1107 44-45 0.6594477760921365 1107 46-47 0.4995481308516574 1107 48-49 0.45580691624277136 1107 50-51 0.3053325667687261 1107 52-53 0.2346108971133063 1107 54-55 0.29347631701100596 1107 56-57 0.37643784405992875 1107 58-59 0.34437984793177634 1107 60-61 0.5301323557015181 1107 62-63 0.5306303679585227 1107 64-65 0.5612888706510759 1107 66-67 0.6997919381097191 1107 68-69 0.6584379430355938 1107 70-71 0.6615371207540655 1107 72-73 0.7117076407107028 1107 74-75 0.7250538104761901 1107 76-77 0.7424019633635943 1107 78-79 0.8911507419308506 1107 80-81 1.168287574973938 1107 82-83 1.1757437540885753 1107 84-85 1.0843934935922839 1107 86-87 1.0034658291686576 1107 88-89 1.2646053808175388 1107 90-91 1.488299375749019 1107 92-93 1.5869551517360634 1107 94-95 1.6985361895100262 1107 96-97 1.5353230440995915 1107 98-99 1.5611839224774116 1107 100-101 1.473243527825737 1108 1 0.1780624685419241 1108 2 0.0060027145020384864 1108 3 0.13534408054622205 1108 4 0.15397408505031507 1108 5 0.22796325385074567 1108 6 0.10804059515243836 1108 7 0.14944472497918326 1108 8 0.22420211161564652 1108 9 0.25180409811628124 1108 10-11 0.31036753461703626 1108 12-13 0.2903863632308443 1108 14-15 0.2136119933672589 1108 16-17 0.2792331488941002 1108 18-19 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68-69 -1.056588062733173 2316 70-71 -0.9908364124394886 2316 72-73 -1.1666079500083057 2316 74-75 -1.0411253686913966 2316 76-77 -0.9382332955936263 2316 78-79 -0.8949283526745475 2316 80-81 -1.0692580364801856 2316 82-83 -0.956063212445649 2316 84-85 -1.0333448733149417 2316 86-87 -0.7359974905343272 2316 88-89 -0.6755367531614027 2316 90-91 -0.6194513858898674 2316 92-93 -0.7020343880371236 2316 94-95 -0.6317631374676296 2316 96-97 -0.39278751227326936 2316 98-99 -0.18747756234193602 2316 100-101 0.23078635915313228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4011.0 3 719.0 4 718.0 5 778.0 6 870.0 7 1090.0 8 1262.0 9 1647.0 10 2105.0 11 2951.0 12 4263.0 13 6078.0 14 7366.0 15 9770.0 16 13328.0 17 18592.0 18 15147.0 19 8593.0 20 7920.0 21 8153.0 22 8805.0 23 9384.0 24 10527.0 25 11553.0 26 13516.0 27 15980.0 28 18830.0 29 22596.0 30 27800.0 31 34476.0 32 43518.0 33 57811.0 34 78981.0 35 112311.0 36 157849.0 37 211950.0 38 163810.0 39 18918.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.862440260161854 19.819163982815095 14.993803774699762 20.324591982323287 2 15.067969920823332 21.01926145983917 41.206968961508025 22.705799657829473 3 19.135967832201217 27.32220580707278 30.128047006858118 23.413779353867888 4 11.924613314329871 17.11761574929577 37.75585267130082 33.20191826507354 5 12.421106986795312 39.70301389436604 33.02250650331311 14.853372615525542 6 29.776180928589795 37.102237130337116 17.816542411102855 15.30503952997023 7 27.566287117384018 35.38423069480757 19.94555483790592 17.1039273499025 8 27.38522816532057 34.45333008184387 19.26746063091265 18.893981121922902 9 28.040284397881333 16.595973681001436 20.502202970165794 34.861538950951434 10-11 24.674079500477625 28.304161832241853 26.019647573744674 21.002111093535845 12-13 26.223285129891273 26.10523227738179 27.49566690362655 20.17581568910039 14-15 23.096060943722843 26.343615215429985 27.235220254721575 23.3251035861256 16-17 20.375309894172133 30.478875952212242 27.492185277398356 21.65362887621727 18-19 20.418866902605295 27.55622391580075 31.91502145050568 20.10988773108828 20-21 21.866199842831264 26.3808033274892 31.991789059942775 19.761207769736757 22-23 21.254760074570818 26.896784796034673 31.921612284747443 19.92684284464707 24-25 20.638808916164777 26.987271267823782 32.39281875615993 19.981101059851504 26-27 20.500671927225113 27.230060636903957 32.064755937124225 20.204511498746708 28-29 20.207238548331656 27.290930121384726 32.3000713092178 20.20176002106582 30-31 20.306844850986824 27.141048252558953 32.41712555844597 20.134981338008256 32-33 20.04802668640164 27.3864336761181 32.028232799089835 20.53730683839042 34-35 20.493587166380227 27.274976473758855 32.013492796331235 20.217943563529687 36-37 20.806817689876745 26.95950715580022 31.779103596762553 20.45457155756048 38-39 20.480768126529924 27.18058552951808 31.924088406282326 20.414557937669667 40-41 20.37777183958941 27.41833825442291 31.848202238009954 20.355687667977733 42-43 20.523698792796356 27.441707189505525 31.430718314438383 20.60387570325973 44-45 20.372946451014112 27.7259428467427 31.19556761642318 20.705543085820004 46-47 20.831166903519843 27.9645042715296 30.484041897611398 20.72028692733916 48-49 20.85960315812262 28.40070337593942 29.731860014049843 21.007833451888114 50-51 21.078818376007018 28.537239976231447 29.435320157543977 20.948621490217562 52-53 21.12463832667872 28.77346568323338 29.396330330362176 20.705565659725718 54-55 20.793499402960418 28.846001778528574 29.281798708203365 21.078700110307647 56-57 20.99919481039176 29.08722062946128 29.001975651737606 20.911608908409352 58-59 21.0918351977873 28.57797822725299 29.138440731388332 21.19174584357138 60-61 20.786397759281783 28.70195921518332 29.608784095917724 20.902858929617175 62-63 20.97183662184329 28.639937530530275 29.816672599735178 20.57155324789126 64-65 20.93064033142827 28.716112961212648 29.60307224818253 20.750174459176552 66-67 20.81288446522014 28.906263801881828 29.37950272592687 20.901349006971166 68-69 20.76736454784799 29.000162975859702 29.336670584270664 20.895801892021648 70-71 20.770352284587258 29.018151527290893 29.30687463092513 20.904621557196723 72-73 20.775735583153203 29.2270027851856 28.850874643680864 21.14638698798033 74-75 20.650744520182155 29.603318657465056 28.828561798775247 20.917375023577538 76-77 20.735837214037765 29.4534975810288 28.941699547566476 20.868965657366964 78-79 20.77925577261672 29.307807222827382 28.736133307536893 21.176803697019007 80-81 20.627379616787838 29.5511082057579 28.699260607238454 21.12225157021581 82-83 20.76334246280132 29.41462475413586 28.8738782103652 20.94815457269762 84-85 20.66955151997972 29.27973023280051 28.68798054821634 21.362737699003432 86-87 20.871517192618 29.08014713123677 28.67120390501035 21.37713177113488 88-89 20.92599168283109 29.070916761179948 28.61283649378686 21.390255062202108 90-91 20.600552614051733 29.16266957813712 28.894224229158166 21.342553578652986 92-93 20.77039231559789 29.321758501702348 28.600009804687964 21.307839378011796 94-95 20.419417468867426 29.07929906880524 28.710866765480603 21.79041669684673 96-97 20.773418193944185 29.082750137471542 28.43392863846862 21.70990303011565 98-99 20.663923032174026 29.224875403450763 28.294801862443997 21.816399701931218 100-101 20.811260683751247 28.79786848314912 28.842694639365845 21.548176193733788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2262.0 1 1375.0 2 598.0 3 728.5 4 686.5 5 735.5 6 1279.5 7 2439.5 8 3796.5 9 4324.5 10 3817.0 11 3309.5 12 3336.5 13 3465.5 14 3523.0 15 3553.5 16 3567.5 17 3649.5 18 3783.5 19 3937.5 20 4176.0 21 4629.0 22 5220.5 23 6015.0 24 6825.0 25 7878.0 26 9509.0 27 11073.0 28 12050.5 29 13563.0 30 16344.5 31 18811.5 32 21308.5 33 24150.0 34 27011.0 35 28851.0 36 31616.5 37 34680.5 38 34901.5 39 34821.5 40 35882.0 41 37209.5 42 37689.5 43 39119.5 44 41670.0 45 43751.0 46 42437.5 47 40030.0 48 40633.0 49 39296.5 50 36058.0 51 32699.0 52 29904.5 53 28739.0 54 27574.0 55 25762.5 56 23366.0 57 21166.5 58 19579.5 59 17704.5 60 15218.0 61 12697.5 62 10362.5 63 7825.5 64 5494.0 65 4126.5 66 3003.0 67 2177.5 68 1545.5 69 1050.5 70 820.5 71 626.5 72 482.5 73 445.0 74 365.0 75 276.0 76 222.5 77 154.5 78 104.0 79 71.0 80 46.5 81 32.5 82 20.5 83 14.0 84 9.5 85 7.5 86 5.5 87 3.5 88 4.0 89 4.5 90 4.5 91 3.5 92 2.5 93 3.0 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30441480838710416 2 0.2613805017553235 3 0.2566185088083642 4 0.26076320637331024 5 0.26605430964770954 6 0.2033547358460783 7 0.19383074995215963 8 0.19312526951557304 9 0.2050302518829714 10-11 0.20617665759242454 12-13 0.20128238706360524 14-15 0.2038838461735182 16-17 0.1888042018414803 18-19 0.19290480687913975 20-21 0.18293989571235444 22-23 0.1912733833695333 24-25 0.19131747589681997 26-27 0.19047971787837342 28-29 0.2017674048637585 30-31 0.19991551871771876 32-33 0.20428067891909815 34-35 0.19965096355399878 36-37 0.18518861460397415 38-39 0.174430037946029 40-41 0.17165220872696937 42-43 0.1858059099859874 44-45 0.18761370360474047 46-47 0.18734914844102052 48-49 0.2022965151911984 50-51 0.19678494928036588 52-53 0.21455423777689006 54-55 0.18911284953248694 56-57 0.17174039378154268 58-59 0.1735040948730091 60-61 0.1624368705240573 62-63 0.16808071401674987 64-65 0.16834526918046983 66-67 0.16640519797985678 68-69 0.16838936170775648 70-71 0.1698003225809296 72-73 0.16913893467162971 74-75 0.18302808076692778 76-77 0.17544416607362218 78-79 0.17989751132957488 80-81 0.1736363724548691 82-83 0.15414747539416515 84-85 0.1537506426485852 86-87 0.15807171032267792 88-89 0.16428875667009707 90-91 0.16799252896217653 92-93 0.16468558941567701 94-95 0.16622882787071014 96-97 0.16988850763550295 98-99 0.17861882803826173 100-101 0.16115818723274417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1133979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.193000080779996 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97898870611155 38.895923749695505 2 13.00417699046745 13.314456674469552 3 4.475391360724795 6.873261308733196 4 2.0055426670251255 4.106789836600998 5 1.1400681654224891 2.9181754842284096 6 0.702722222138308 2.158467526481538 7 0.4636456044405939 1.661478662590643 8 0.3217259294388485 1.3176092425401593 9 0.24979604705685113 1.1509028151443914 >10 1.4650739408510007 13.909054237397125 >50 0.12467307635784775 4.379587434808801 >100 0.06456051652909017 5.680160513643704 >500 0.002250097841448311 0.7528947181113741 >1k 8.654222467108888E-4 0.6844014125799992 >5k 3.4616889868435555E-4 1.2338541779099546 >10k+ 1.7308444934217778E-4 0.9629822050646596 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10868 0.9583951731028528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8202 0.723293817610379 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5723 0.5046830673231162 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.17116719092681612 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1709 0.1507082582658056 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1472 0.12980840033192856 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1471 0.12972021527735522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.409252728666051E-5 0.0 12-13 8.818505457332102E-5 0.0 0.0 8.818505457332102E-5 0.0 14-15 1.7637010914664205E-4 0.0 0.0 8.818505457332102E-5 0.0 16-17 1.7637010914664205E-4 0.0 0.0 8.818505457332102E-5 0.0 18-19 2.645551637199631E-4 0.0 0.0 8.818505457332102E-5 0.0 20-21 3.527402182932841E-4 0.0 0.0 8.818505457332102E-5 0.0 22-23 3.527402182932841E-4 0.0 0.0 8.818505457332102E-5 0.0 24-25 3.527402182932841E-4 0.0 0.0 1.7637010914664205E-4 0.0 26-27 3.9683274557994464E-4 0.0 0.0 5.732028547265867E-4 0.0 28-29 5.732028547265867E-4 0.0 0.0 0.0016755160368930994 0.0 30-31 6.172953820132471E-4 0.0 0.0 0.004100605037659428 0.0 32-33 6.172953820132471E-4 0.0 0.0 0.006128861292845811 0.0 34-35 6.172953820132471E-4 0.0 0.0 0.00864213534818546 0.0 36-37 6.172953820132471E-4 0.0 0.0 0.012213630058404961 0.0 38-39 7.936654911598892E-4 0.0 0.0 0.018651139042257396 0.0 40-41 7.936654911598892E-4 0.0 0.0 0.02654370142656963 0.0 42-43 7.936654911598892E-4 0.0 0.0 0.0377432033573814 0.0 44-45 7.936654911598892E-4 0.0 0.0 0.046208968596420215 0.0 46-47 9.700356003065312E-4 0.0 0.0 0.06433099731123769 0.0 48-49 0.0010582206548798523 0.0 0.0 0.08814096204603436 0.0 50-51 0.0010582206548798523 0.0 0.0 0.10039868463172599 0.0 52-53 0.0010582206548798523 0.0 0.0 0.12390000167551604 0.0 54-55 0.0011464057094531734 0.0 0.0 0.16283370326963725 0.0 56-57 0.0011464057094531734 0.0 0.0 0.22372548345251542 0.0 58-59 0.0011464057094531734 0.0 0.0 0.2862045946177134 0.0 60-61 0.0011464057094531734 0.0 0.0 0.37130317228096815 0.0 62-63 0.0011464057094531734 0.0 0.0 0.45481441896190317 0.0 64-65 0.0011464057094531734 0.0 0.0 0.5426026407896443 0.0 66-67 0.0012345907640264942 0.0 0.0 0.6582132473352681 0.0 68-69 0.0012345907640264942 0.0 0.0 0.8131102956933065 0.0 70-71 0.0012345907640264942 0.0 0.0 0.9103343183603929 0.0 72-73 0.0012345907640264942 0.0 0.0 1.0365271314548155 0.0 74-75 0.0012345907640264942 0.0 0.0 1.1740076315346228 0.0 76-77 0.0012345907640264942 8.818505457332102E-5 0.0 1.3167792348888294 0.0 78-79 0.0012345907640264942 8.818505457332102E-5 0.0 1.485433151760306 0.0 80-81 0.0015873309823197783 8.818505457332102E-5 0.0 1.737906963003724 0.0 82-83 0.0015873309823197783 8.818505457332102E-5 0.0 2.011942020090319 0.0 84-85 0.001631423509606439 8.818505457332102E-5 0.0 2.34175412419454 0.0 86-87 0.0017196085641797598 8.818505457332102E-5 0.0 2.751505980269476 0.0 88-89 0.0017637010914664205 8.818505457332102E-5 0.0 3.1971491535557535 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4860 0.0 48.819572 1 GTATCAA 8905 0.0 42.76892 1 ATCAACG 11375 0.0 33.176144 3 TATCAAC 11480 0.0 32.789898 2 TCAACGC 11545 0.0 32.690514 4 CAACGCA 11655 0.0 32.339767 5 TATCACG 90 2.402955E-5 31.685863 2 AACGCAG 11940 0.0 31.549696 6 GTACACG 180 2.0918378E-10 29.05694 1 CGCAGAG 13485 0.0 27.61795 8 ACGCAGA 13535 0.0 27.58612 7 GCAGAGT 14085 0.0 26.03675 9 GTACATG 9850 0.0 25.632324 1 GAGTACT 7300 0.0 24.955648 12-13 TACATGG 9965 0.0 24.944866 2 GTAAGGT 305 0.0 24.93534 4 ACATGGG 9915 0.0 23.68051 3 AGTACTT 7630 0.0 23.409367 12-13 CATGGGT 800 0.0 23.172337 4 GTACTTT 8105 0.0 22.33 14-15 >>END_MODULE