FastQCFastQC Report
Thu 26 May 2016
SRR937578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133979
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164881.453995179804917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133791.1798278451364619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96230.8486047801590683No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.27681288630565465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.24462534138639253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13340.11763886280081023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.11675701225507702No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.10740939647030501No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA11580.10211829319590575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.052.46761
GTATCAA107550.042.380591
GCGTATC550.002571898534.5665551
TATCAAC141250.032.2550242
ATCAACG142700.031.860693
TCAACGC144700.031.4531524
CAACGCA147550.030.8134235
AACGCAG150500.030.2658986
CGTATCA700.00838459227.1474552
ACGCAGA169550.026.6996087
CGCAGAG170550.026.45958
GCAGAGT176450.025.2786399
GAGTACT108800.024.3184612-13
AGTACTT112150.023.14731412-13
GTACATG89800.023.0764451
GTGGTAT28400.022.5930181
GTACTTT115950.022.5915614-15
CATGGGT8250.022.4583514
GGACCGA1703.1503187E-622.35186
TACATGG92200.022.3112642
Produced by FastQC (version 0.11.5)