##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937578_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1133979 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25890250172181 33.0 31.0 34.0 31.0 34.0 2 32.45010269149605 34.0 31.0 34.0 31.0 34.0 3 32.49876232275906 34.0 31.0 34.0 31.0 34.0 4 36.13627236483215 37.0 35.0 37.0 35.0 37.0 5 36.072214741190095 37.0 35.0 37.0 35.0 37.0 6 36.13525559115292 37.0 35.0 37.0 35.0 37.0 7 36.0840562303182 37.0 35.0 37.0 35.0 37.0 8 36.074589564709754 37.0 35.0 37.0 35.0 37.0 9 37.73777821282405 39.0 37.0 39.0 35.0 39.0 10-11 37.688634886536704 39.0 37.0 39.0 35.0 39.0 12-13 37.579524400363674 39.0 37.0 39.0 35.0 39.0 14-15 38.71531307017149 40.0 38.0 41.0 34.5 41.0 16-17 38.700565883495194 40.0 38.0 41.0 34.5 41.0 18-19 38.795366580862606 40.0 38.0 41.0 35.0 41.0 20-21 38.75189134895796 40.0 38.0 41.0 35.0 41.0 22-23 38.60239916259472 40.0 38.0 41.0 34.0 41.0 24-25 38.64299823894446 40.0 38.0 41.0 34.0 41.0 26-27 38.496538295682726 40.0 38.0 41.0 34.0 41.0 28-29 38.336345293872284 40.0 38.0 41.0 34.0 41.0 30-31 38.2340982504967 40.0 37.5 41.0 34.0 41.0 32-33 38.02675402278173 40.0 37.0 41.0 34.0 41.0 34-35 37.8508336574134 40.0 36.0 41.0 33.0 41.0 36-37 37.642932982004076 40.0 36.0 41.0 33.0 41.0 38-39 37.4628291176468 40.0 36.0 41.0 33.0 41.0 40-41 37.221897848196484 40.0 36.0 41.0 33.0 41.0 42-43 36.89595133595948 40.0 35.0 41.0 32.0 41.0 44-45 36.39348083165561 39.0 35.0 41.0 30.5 41.0 46-47 35.75766438355561 39.0 35.0 41.0 29.0 41.0 48-49 35.190689157382984 39.0 35.0 41.0 25.0 41.0 50-51 34.86324482199406 39.0 35.0 40.5 23.5 41.0 52-53 34.59725444651092 39.0 35.0 40.0 23.0 41.0 54-55 34.37193898652444 38.0 34.5 40.0 22.0 41.0 56-57 34.11523582006369 38.0 34.0 40.0 20.5 41.0 58-59 33.844697741316196 38.0 34.0 40.0 19.5 41.0 60-61 33.50193610287316 37.5 33.5 40.0 18.0 41.0 62-63 33.16562872857434 37.0 33.0 40.0 15.0 41.0 64-65 32.77007466628571 36.5 33.0 39.5 10.0 41.0 66-67 32.34068576225839 36.0 32.0 39.0 7.5 40.5 68-69 31.900429813955988 35.5 32.0 39.0 6.0 40.0 70-71 31.48539479126157 35.0 31.0 38.0 2.0 40.0 72-73 31.01152578663273 35.0 31.0 37.5 2.0 39.5 74-75 30.511839725427016 35.0 30.5 37.0 2.0 39.0 76-77 29.28340119173283 33.5 28.5 35.5 2.0 38.0 78-79 29.862267731589384 34.0 30.0 36.0 2.0 38.5 80-81 29.662507418567714 34.0 30.0 36.0 2.0 37.0 82-83 29.300838022573608 34.0 29.5 35.0 2.0 37.0 84-85 28.91479119101853 34.0 29.0 35.0 2.0 36.5 86-87 28.490884310908754 34.0 29.0 35.0 2.0 36.0 88-89 28.127289835173315 34.0 29.0 35.0 2.0 36.0 90-91 27.61524992967242 33.5 27.0 35.0 2.0 35.0 92-93 27.1574522103143 33.0 26.0 35.0 2.0 35.0 94-95 26.70556509423896 33.0 25.0 35.0 2.0 35.0 96-97 26.17763865115668 33.0 24.5 35.0 2.0 35.0 98-99 25.340038043032543 32.5 21.0 34.5 2.0 35.0 100-101 22.80226441583133 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29650069835172843 1101 2 0.2997973879335518 1101 3 0.2414144670344811 1101 4 0.17110120017414943 1101 5 0.13688323942040626 1101 6 0.13428013853162923 1101 7 0.19293636573527806 1101 8 0.17830006123712394 1101 9 0.2538150340371743 1101 10-11 0.26336307073487575 1101 12-13 0.2642256651357826 1101 14-15 0.29594132280372065 1101 16-17 0.3895290926019399 1101 18-19 0.3148472972429204 1101 20-21 0.4102322492038084 1101 22-23 0.3087789252149875 1101 24-25 0.3916112689168827 1101 26-27 0.3708604000788398 1101 28-29 0.3260464988106193 1101 30-31 0.37044921889788185 1101 32-33 0.46285701424934445 1101 34-35 0.475934750372069 1101 36-37 0.5347142687366429 1101 38-39 0.5772338613220853 1101 40-41 0.636867988683079 1101 42-43 0.8797044208822911 1101 44-45 0.847236939015346 1101 46-47 0.7033306406356346 1101 48-49 0.752361014555035 1101 50-51 0.7569638031183317 1101 52-53 0.6347845062431432 1101 54-55 0.7313269370282214 1101 56-57 0.8331817673764448 1101 58-59 0.9027126992775933 1101 60-61 0.9744720943266785 1101 62-63 1.0053256769126406 1101 64-65 1.0338201281519233 1101 66-67 0.7837207622298479 1101 68-69 0.8934196951926445 1101 70-71 1.0121299773103694 1101 72-73 0.9227309727069866 1101 74-75 0.9274259009453196 1101 76-77 0.7311088258870626 1101 78-79 0.881411384292516 1101 80-81 0.9155424191893928 1101 82-83 0.8366090839342704 1101 84-85 0.711656515967185 1101 86-87 0.6639932655507756 1101 88-89 0.9941901302332141 1101 90-91 1.1359015710258333 1101 92-93 0.9612950441751344 1101 94-95 0.8376962646304769 1101 96-97 1.2713420459764357 1101 98-99 1.2924717842877627 1101 100-101 1.4142565326750507 1102 1 0.2867049148292935 1102 2 0.30917085880332706 1102 3 0.2673277376252088 1102 4 0.1334114841365519 1102 5 0.07561362110170222 1102 6 0.13132083326451038 1102 7 0.19131724084189727 1102 8 0.1999200795840892 1102 9 0.18116427153687908 1102 10-11 0.2950334029853323 1102 12-13 0.24276511786429467 1102 14-15 0.38849350412908024 1102 16-17 0.4397754676218142 1102 18-19 0.39497294175020414 1102 20-21 0.27762763461993956 1102 22-23 0.32469019072966177 1102 24-25 0.32574244244738537 1102 26-27 0.26023786032276064 1102 28-29 0.3097630475448696 1102 30-31 0.3458943094223912 1102 32-33 0.41117176340186035 1102 34-35 0.5851511396352507 1102 36-37 0.5755892506857592 1102 38-39 0.498107378422894 1102 40-41 0.5453230204370456 1102 42-43 0.6174228105464863 1102 44-45 0.5171133723070866 1102 46-47 0.5895119265231159 1102 48-49 0.5182520215291646 1102 50-51 0.3034680620868855 1102 52-53 0.15195024292159331 1102 54-55 0.1496161915866523 1102 56-57 0.09755701995889154 1102 58-59 0.16008602885703027 1102 60-61 0.10111836046987577 1102 62-63 0.2296988828630404 1102 64-65 0.2516448907374169 1102 66-67 0.3245063988207235 1102 68-69 0.17332649551719115 1102 70-71 0.05010798796324423 1102 72-73 0.25862199007011455 1102 74-75 0.07758220337727906 1102 76-77 0.06725203610734098 1102 78-79 0.23362221440439512 1102 80-81 0.41794851046502757 1102 82-83 0.5351396644609316 1102 84-85 0.38646018993143016 1102 86-87 0.36364793535214446 1102 88-89 0.4449883686751974 1102 90-91 0.306021885978609 1102 92-93 0.48246210233376985 1102 94-95 0.5572219654461179 1102 96-97 0.5762725591824065 1102 98-99 0.46494183021750857 1102 100-101 1.200955398300671 1103 1 0.3197324752035229 1103 2 0.2076740675937785 1103 3 0.21950408793749432 1103 4 0.18401436732283116 1103 5 0.13938457274218052 1103 6 0.1603417011413839 1103 7 0.2215848977805308 1103 8 0.1737385831794782 1103 9 0.29385316920782145 1103 10-11 0.16570185804079074 1103 12-13 0.21524018208306472 1103 14-15 0.25418070934562564 1103 16-17 0.2911611109692629 1103 18-19 0.08901348333561288 1103 20-21 0.09486994340266364 1103 22-23 0.3208923703016495 1103 24-25 0.25604306788230957 1103 26-27 0.1377507685092496 1103 28-29 0.27019440863904265 1103 30-31 0.3225639973063039 1103 32-33 0.3428786509180739 1103 34-35 0.3384034227304653 1103 36-37 0.22353349848857818 1103 38-39 0.2720809520790226 1103 40-41 0.2433348160710267 1103 42-43 0.1587478004518985 1103 44-45 0.23744602121301028 1103 46-47 0.36416366322188765 1103 48-49 0.4662691866250839 1103 50-51 0.5633484089970864 1103 52-53 0.3819167804348069 1103 54-55 0.5628397802772724 1103 56-57 0.5118287276196085 1103 58-59 0.45263042356832983 1103 60-61 0.42872587857569755 1103 62-63 0.535068237410762 1103 64-65 0.5326025235349476 1103 66-67 0.43359507226293204 1103 68-69 0.33632886237601767 1103 70-71 0.5388825908378401 1103 72-73 0.5963869193220823 1103 74-75 0.6901041107819843 1103 76-77 0.44067448819026467 1103 78-79 0.30747164610054156 1103 80-81 0.4327617820567049 1103 82-83 0.5971558759404125 1103 84-85 0.7201529192504097 1103 86-87 0.6805272685079267 1103 88-89 0.5217077777766512 1103 90-91 0.5074384574268151 1103 92-93 0.6203053183407796 1103 94-95 0.5543391013321646 1103 96-97 0.6667651750983516 1103 98-99 0.8785181925573937 1103 100-101 1.1923760720177974 1104 1 0.2579866171970977 1104 2 0.2176895243883621 1104 3 0.12479323209169024 1104 4 0.07488547997461126 1104 5 0.12200992473447059 1104 6 0.1857065147338659 1104 7 0.19616013271905786 1104 8 0.17466456626485893 1104 9 0.29846943231997614 1104 10-11 0.31998399558963797 1104 12-13 0.3043903907854002 1104 14-15 0.30721876487611866 1104 16-17 0.22237154893552002 1104 18-19 0.24579844632003045 1104 20-21 0.20243623429431779 1104 22-23 0.28299740290099606 1104 24-25 0.26500462533837066 1104 26-27 0.21768150972309286 1104 28-29 0.14300469607368882 1104 30-31 0.2723003295585329 1104 32-33 0.3500289023580976 1104 34-35 0.3454999552799549 1104 36-37 0.5200697875282643 1104 38-39 0.3541372993369265 1104 40-41 0.3156724959847921 1104 42-43 0.3502081588785799 1104 44-45 0.33046577077019634 1104 46-47 0.2550048001391687 1104 48-49 0.48491468705958596 1104 50-51 0.4771960705336511 1104 52-53 0.5468515817283333 1104 54-55 0.507089365959196 1104 56-57 0.5808367266888723 1104 58-59 0.6207990427138625 1104 60-61 0.43867099627052397 1104 62-63 0.6304614122031253 1104 64-65 0.6942711569263764 1104 66-67 0.6361808800288387 1104 68-69 0.6237430308669509 1104 70-71 0.7304572132029428 1104 72-73 0.5936362746953954 1104 74-75 0.32459870209104125 1104 76-77 0.2268365019897871 1104 78-79 0.5398126737993074 1104 80-81 0.4844138308536401 1104 82-83 0.7306677973790556 1104 84-85 0.584854918899012 1104 86-87 0.6702622117855306 1104 88-89 0.8423552417701536 1104 90-91 0.7114993087614181 1104 92-93 0.5569867730364635 1104 94-95 0.5294696293324677 1104 96-97 0.7888631678519396 1104 98-99 1.02209483783939 1104 100-101 1.301526328206453 1105 1 0.15654952048330273 1105 2 0.15113945661689598 1105 3 0.06976069981729438 1105 4 0.07447681442531717 1105 5 -0.006236157628066508 1105 6 -0.005876814602601144 1105 7 0.07743884853684335 1105 8 0.08327328345581009 1105 9 0.0378235962079998 1105 10-11 0.17564851402459425 1105 12-13 0.159349271278316 1105 14-15 0.18622321092076533 1105 16-17 0.13030025190860073 1105 18-19 0.17174259842771988 1105 20-21 0.21974374592189605 1105 22-23 0.19445396707495632 1105 24-25 0.2591390920725374 1105 26-27 0.07120841824706048 1105 28-29 0.13693483003320495 1105 30-31 0.25136443288916155 1105 32-33 0.29324180199785843 1105 34-35 0.29576200713580647 1105 36-37 0.2794104562549009 1105 38-39 0.18026127742667342 1105 40-41 0.18041405444597558 1105 42-43 0.30908930881876273 1105 44-45 0.39780426082326414 1105 46-47 0.3819624690898564 1105 48-49 0.3993268492616977 1105 50-51 0.7404009939679881 1105 52-53 0.8019088179008875 1105 54-55 0.7055753323710974 1105 56-57 0.781731651680353 1105 58-59 0.7359645424060446 1105 60-61 0.66545343836912 1105 62-63 0.5909755817940763 1105 64-65 0.7713318792394475 1105 66-67 0.7465465305724948 1105 68-69 0.6085064417657087 1105 70-71 0.8297773960906305 1105 72-73 0.7904333748782442 1105 74-75 0.7549823286627095 1105 76-77 0.7122092976103644 1105 78-79 1.0095835779948317 1105 80-81 0.8276823799661059 1105 82-83 0.9194729237007309 1105 84-85 1.0807738480638704 1105 86-87 1.1190988618580953 1105 88-89 1.070869913323289 1105 90-91 1.239196255004817 1105 92-93 1.4648514166991582 1105 94-95 1.2952339685984597 1105 96-97 1.272697883004824 1105 98-99 1.3244554398523647 1105 100-101 1.5590191322105902 1106 1 -0.07356101428309358 1106 2 0.032325317345765825 1106 3 -0.037006043880431605 1106 4 -0.009406564814590013 1106 5 0.08065220739640466 1106 6 -0.016065891489837725 1106 7 -0.03296780649655773 1106 8 -0.03673257306368072 1106 9 0.0929192257099345 1106 10-11 -0.11091197037714551 1106 12-13 -0.24643608305871112 1106 14-15 -0.014274945190670962 1106 16-17 0.07191173800745077 1106 18-19 0.08003844276255734 1106 20-21 -0.0683593541619345 1106 22-23 0.014498679348804444 1106 24-25 -0.030059641736677634 1106 26-27 0.11090323163737281 1106 28-29 -0.018334735282117265 1106 30-31 0.0033703964027651523 1106 32-33 0.026646628980643072 1106 34-35 0.054980570050098265 1106 36-37 -0.234311537861835 1106 38-39 -0.10169740454671938 1106 40-41 -0.14388543331973835 1106 42-43 -0.243516595988865 1106 44-45 -0.40403948695502123 1106 46-47 -0.4372607818556702 1106 48-49 -0.3376344758884642 1106 50-51 -0.13083406293245758 1106 52-53 -0.34397659000358516 1106 54-55 -0.307031670533938 1106 56-57 -0.33857019840260705 1106 58-59 -0.6135475433525457 1106 60-61 -0.7318973312493355 1106 62-63 -0.47368581353577355 1106 64-65 -0.42093019437672297 1106 66-67 -0.3266286391926627 1106 68-69 -0.3541638777172871 1106 70-71 -0.19115966110686955 1106 72-73 -0.15473742502236476 1106 74-75 -0.37476667865019664 1106 76-77 -0.5081932817922841 1106 78-79 -0.56080200201529 1106 80-81 -0.6207450530411514 1106 82-83 -0.7404991029717714 1106 84-85 -0.5352207853370174 1106 86-87 -0.5479534293995698 1106 88-89 -0.6646360808103395 1106 90-91 -0.4033857098715501 1106 92-93 -0.36913455721821364 1106 94-95 -0.2899973955975099 1106 96-97 -0.08196294215906264 1106 98-99 -0.253201163971692 1106 100-101 0.037400339540528904 1107 1 0.2805448184528956 1107 2 0.3360610811418354 1107 3 0.3501591105575841 1107 4 0.14031769253613646 1107 5 0.14690110280830027 1107 6 0.1914698256538827 1107 7 0.22796442329862998 1107 8 0.18261861869962104 1107 9 0.2975476839858757 1107 10-11 0.34318781798462794 1107 12-13 0.37782433241966373 1107 14-15 0.3748456920055574 1107 16-17 0.40000079632800833 1107 18-19 0.3015328334130132 1107 20-21 0.3789673297922107 1107 22-23 0.2431433093054025 1107 24-25 0.30366600837407276 1107 26-27 0.29417589420975787 1107 28-29 0.3576687585364411 1107 30-31 0.4325669373180716 1107 32-33 0.3486176738741662 1107 34-35 0.45370963395530595 1107 36-37 0.342619724833483 1107 38-39 0.43963462229370265 1107 40-41 0.49828633587711124 1107 42-43 0.2433683889342504 1107 44-45 0.5269141790856366 1107 46-47 0.46597432046451104 1107 48-49 0.4936712962335221 1107 50-51 0.505790928767162 1107 52-53 0.5681650254277812 1107 54-55 0.6846590175542175 1107 56-57 0.42852814441727105 1107 58-59 0.5143871705953202 1107 60-61 0.6700790939885692 1107 62-63 0.7994675450079924 1107 64-65 1.032850774527951 1107 66-67 0.7556621105310839 1107 68-69 0.8631437196354419 1107 70-71 0.7889500911078393 1107 72-73 0.7393344911123698 1107 74-75 0.7803047215844252 1107 76-77 0.5871260739966075 1107 78-79 0.8159676800846789 1107 80-81 0.6898898105688183 1107 82-83 0.8486813820424324 1107 84-85 0.68887779414991 1107 86-87 0.5230168884664117 1107 88-89 0.5703747224444307 1107 90-91 0.43302840978662616 1107 92-93 0.7566446136979614 1107 94-95 0.974916033639893 1107 96-97 0.9681193805151729 1107 98-99 1.1781968912721865 1107 100-101 1.164210459355349 1108 1 0.02219377117186383 1108 2 0.05754046662572421 1108 3 0.0650807143897083 1108 4 0.057551118625084996 1108 5 -0.005124544322910651 1108 6 -0.07403516524725973 1108 7 -0.03505684908134299 1108 8 -0.03836106834141617 1108 9 0.013821515256395855 1108 10-11 0.1062581911681093 1108 12-13 0.13645521082868584 1108 14-15 0.04305287827749282 1108 16-17 0.018763473460239766 1108 18-19 -0.10829593079363065 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 9.0 5 37.0 6 52.0 7 148.0 8 331.0 9 623.0 10 1107.0 11 1954.0 12 3338.0 13 4986.0 14 6565.0 15 9880.0 16 14890.0 17 22365.0 18 19971.0 19 9771.0 20 6988.0 21 6796.0 22 6865.0 23 7169.0 24 8049.0 25 9084.0 26 10749.0 27 12974.0 28 16054.0 29 19557.0 30 24350.0 31 30811.0 32 39542.0 33 52107.0 34 72745.0 35 108871.0 36 160288.0 37 238694.0 38 189799.0 39 16455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42036501938703 19.884489322142134 15.877778836214043 19.817366822256798 2 15.625421634792177 21.444488830921912 40.57085713227494 22.359232402010974 3 19.192242537119295 27.01619694897348 31.133998072274704 22.657562441632518 4 12.188077888704974 17.92611313986086 37.37677219202858 32.509036779405584 5 12.1582072595896 39.361557622286824 33.17532953230404 15.304905585819526 6 29.664923248137754 37.486408478463886 17.13947083676153 15.709197436636835 7 26.954996521099595 35.88629066323098 19.85169037521859 17.307022440450837 8 27.731113186399398 33.99577946328812 18.889062319496215 19.384045030816267 9 27.88552521695728 16.13292662386164 20.46431194933945 35.51723620984163 10-11 24.79155257725231 28.212691769424303 25.690070098299884 21.30568555502351 12-13 26.59937306895051 26.10172615485964 27.572663901373666 19.726236874816184 14-15 23.192184334983278 26.014899746820706 27.99302279848216 22.799893119713857 16-17 19.919284219549038 29.92678876769323 28.388003657916062 21.765923354841668 18-19 19.895341977232384 26.192063521458508 34.156981743048156 19.755612758260956 20-21 21.13346014344181 25.00099208186395 34.567306801977814 19.298240972716428 22-23 20.599943208574448 25.56822290458159 34.491311088261284 19.340522798582683 24-25 20.22582438732304 25.510889315802725 34.93712623299295 19.326160063881282 26-27 20.089860887585704 25.83914989307524 34.55446548645252 19.51652373288653 28-29 19.660051835723614 25.87857494971194 34.90749730812273 19.55387590644172 30-31 19.813991264388495 25.636453585119302 34.98750858701969 19.562046563472517 32-33 19.652877169683038 25.78010703901924 34.661797087953126 19.905218703344595 34-35 19.978059558422157 25.630633371517465 34.71550178618828 19.6758052838721 36-37 20.29067557688458 25.47088614515789 34.39578687083271 19.842651407124823 38-39 20.06406652689317 25.66871917652687 34.433884270190546 19.83333002638941 40-41 19.971039849308447 25.831325641197488 34.3807430871309 19.816891422363163 42-43 20.198368406406953 25.81849789138203 33.95075584351554 20.032377858695476 44-45 20.103635076134566 26.08143537049628 33.562526290169394 20.25240326319976 46-47 20.5345513453071 26.599566658641827 32.68852421429321 20.177357781757863 48-49 20.668592628258548 27.033922144942718 31.717430393331796 20.580054833466935 50-51 20.88151544252583 27.187937342755024 31.241760208963303 20.688787005755838 52-53 20.94029078139895 27.542529447194347 30.909037998058164 20.60814177334854 54-55 20.717358963437594 27.624982473220406 30.778039099489497 20.879619463852507 56-57 20.867714481485105 27.81687315197195 30.542849558942446 20.772562807600494 58-59 21.03023953706374 27.23498406936989 30.80021764071469 20.934558752851686 60-61 20.82432743463503 27.274005956018588 31.221433553884154 20.680233055462228 62-63 20.96687857535281 27.298036383389817 31.455653058830897 20.279431982426484 64-65 20.86912544235828 27.309720903120784 31.2460812766374 20.57507237788354 66-67 20.837907933039325 27.635167847023624 30.846206146674675 20.68071807326238 68-69 20.946315072389 27.49362310980597 30.998895480730436 20.56116633707459 70-71 20.74831191759283 27.787727991435467 30.85709700091448 20.606863090057224 72-73 20.865950780393643 27.799412511166434 30.40237076700715 20.932265941432778 74-75 20.759158679305347 28.050739916700394 30.235039625954276 20.955061778039983 76-77 20.70069198812324 27.895754683287787 30.677596322330487 20.725957006258493 78-79 20.70157383866897 27.928956356334638 30.333365961803526 21.036103843192862 80-81 20.60712764522094 28.131517426689562 30.39015713694874 20.87119779114075 82-83 20.674348639516523 28.069184494778664 30.33907475041966 20.91739211528515 84-85 20.517983885866823 27.8797520400609 30.26208284408763 21.34018122998465 86-87 20.72256188165742 27.656905462975946 30.0871973819621 21.533335273404532 88-89 20.67026814429544 27.599805640139717 30.216476671966593 21.513449543598252 90-91 20.3801739119698 27.608161400609166 30.506341450077812 21.505323237343227 92-93 20.379531223337153 27.587511458823418 30.46426588791496 21.56869142992447 94-95 20.037143544986282 27.468983111680203 30.517628633334482 21.976244709999037 96-97 20.502055152696833 27.26329147188793 30.241521227465412 21.99313214794983 98-99 20.360498360609476 27.464288800819787 30.084535758819992 22.090677079750748 100-101 20.106441951959116 27.25586067993898 30.576747266956467 22.060950101145433 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 968.0 1 687.5 2 727.5 3 1025.5 4 766.0 5 626.5 6 1375.0 7 3443.0 8 5725.0 9 6462.0 10 5272.0 11 3925.0 12 3640.0 13 3691.5 14 3877.5 15 4070.5 16 4135.0 17 4215.0 18 4420.5 19 4562.0 20 4730.0 21 5108.5 22 5707.5 23 6449.5 24 7177.5 25 8303.5 26 9849.0 27 11160.0 28 12068.0 29 13484.0 30 16135.0 31 18357.5 32 20685.5 33 23189.5 34 25637.5 35 27863.0 36 31078.5 37 34159.5 38 34754.5 39 34619.5 40 35140.5 41 36484.5 42 37020.0 43 38650.0 44 41214.0 45 43356.5 46 42518.5 47 40294.0 48 41056.5 49 39293.5 50 35914.0 51 32795.5 52 29804.5 53 28667.0 54 27383.0 55 25459.0 56 22841.5 57 20604.0 58 19290.0 59 17514.5 60 14897.0 61 12345.0 62 10083.5 63 7563.0 64 5232.5 65 3849.0 66 2873.5 67 2156.0 68 1504.5 69 979.5 70 695.5 71 530.0 72 463.0 73 455.5 74 390.0 75 315.0 76 236.5 77 139.5 78 88.5 79 67.0 80 50.0 81 37.5 82 29.0 83 21.0 84 12.0 85 5.5 86 3.5 87 3.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020370747606437156 2 0.0 3 0.0 4 1.7637010914664205E-4 5 0.022134448697903576 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037478648193661436 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.4092527286660507E-4 24-25 4.409252728666051E-5 26-27 3.527402182932841E-4 28-29 0.0010582206548798523 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.3227758185998154E-4 40-41 6.172953820132471E-4 42-43 0.015829217295911125 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.3227758185998154E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0022487188916196863 84-85 4.8501780015326566E-4 86-87 0.0 88-89 0.0 90-91 0.011419964567245072 92-93 0.002425089000766328 94-95 0.0 96-97 0.0 98-99 0.0018518861460397416 100-101 0.01900387926055068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1133979.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.92294215969702 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56899356156448 35.73565565357675 2 13.472807541060325 12.913131530379276 3 4.751528853406241 6.831217272357566 4 2.2353768636372306 4.2850334456484775 5 1.2389574704091926 2.968724359637214 6 0.7564139744746303 2.174974988852055 7 0.5185714877771599 1.7396029990089008 8 0.3732433058836204 1.4309533887483878 9 0.2815233781951451 1.2142285712863603 >10 1.5890931684872212 14.286335118668472 >50 0.1331885449502856 4.440640714563634 >100 0.07510912151805949 6.377069804193211 >500 0.0033381826948052362 1.023665681094799 >1k 0.0012981821590909253 1.0690809583164904 >5k 1.8545459415584647E-4 0.8552469915936143 >10k+ 3.7090918831169295E-4 2.6544385220748703 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16488 1.453995179804917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13379 1.1798278451364619 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9623 0.8486047801590683 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3139 0.27681288630565465 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2774 0.24462534138639253 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1334 0.11763886280081023 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1324 0.11675701225507702 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1218 0.10740939647030501 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1158 0.10211829319590575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 4.409252728666051E-5 0.0 0.0 0.0 0.0 20-21 8.818505457332102E-5 0.0 0.0 0.0 0.0 22-23 8.818505457332102E-5 0.0 0.0 0.0 0.0 24-25 8.818505457332102E-5 0.0 0.0 8.818505457332102E-5 0.0 26-27 8.818505457332102E-5 0.0 0.0 4.850178001532656E-4 0.0 28-29 1.7637010914664205E-4 0.0 0.0 0.0015873309823197783 0.0 30-31 1.7637010914664205E-4 0.0 0.0 0.004144697564946088 0.0 32-33 1.7637010914664205E-4 0.0 0.0 0.006481601511139095 0.0 34-35 1.7637010914664205E-4 0.0 0.0 0.009171245675625387 0.0 36-37 1.7637010914664205E-4 0.0 0.0 0.012742740385844888 0.0 38-39 2.645551637199631E-4 0.0 0.0 0.019136156842410663 0.0 40-41 2.645551637199631E-4 0.0 0.0 0.026940534172149574 0.0 42-43 3.0864769100662356E-4 0.0 0.0 0.03783138841195472 0.0 44-45 3.527402182932841E-4 0.0 0.0 0.04629715365099354 0.0 46-47 5.291103274399261E-4 0.0 0.0 0.06455145994767099 0.0 48-49 7.054804365865682E-4 0.0 0.0 0.08783231435502774 0.0 50-51 7.054804365865682E-4 0.0 0.0 0.1000459444134327 0.0 52-53 7.054804365865682E-4 0.0 0.0 0.12354726145722275 0.0 54-55 7.054804365865682E-4 0.0 0.0 0.16296598085149727 0.0 56-57 9.700356003065312E-4 0.0 0.0 0.22504825927111527 0.0 58-59 9.700356003065312E-4 0.0 0.0 0.2874832779090265 0.0 60-61 9.700356003065312E-4 0.0 0.0 0.37293459579057464 0.0 62-63 9.700356003065312E-4 0.0 0.0 0.4566663051079429 0.0 64-65 9.700356003065312E-4 0.0 0.0 0.5452481924268439 0.0 66-67 0.0010582206548798523 0.0 0.0 0.6612997242453343 0.0 68-69 0.0010582206548798523 0.0 0.0 0.8167258829308126 0.0 70-71 0.0010582206548798523 0.0 0.0 0.913949905597899 0.0 72-73 0.001102313182166513 0.0 0.0 1.0406718290197614 0.0 74-75 0.0011464057094531734 0.0 0.0 1.1786814394270086 0.0 76-77 0.0012345907640264942 0.0 0.0 1.323216743872682 0.0 78-79 0.0013227758185998153 0.0 0.0 1.495177600290658 0.0 80-81 0.0014991459277464575 0.0 0.0 1.7504292407531357 0.0 82-83 0.0014991459277464575 0.0 0.0 2.0286530879319633 0.0 84-85 0.0015873309823197785 0.0 0.0 2.3639767579470163 0.0 86-87 0.0016755160368930994 0.0 0.0 2.7805188632240982 0.0 88-89 0.0016755160368930994 0.0 0.0 3.2339223213128285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6015 0.0 52.4676 1 GTATCAA 10755 0.0 42.38059 1 GCGTATC 55 0.0025718985 34.566555 1 TATCAAC 14125 0.0 32.255024 2 ATCAACG 14270 0.0 31.86069 3 TCAACGC 14470 0.0 31.453152 4 CAACGCA 14755 0.0 30.813423 5 AACGCAG 15050 0.0 30.265898 6 CGTATCA 70 0.008384592 27.147455 2 ACGCAGA 16955 0.0 26.699608 7 CGCAGAG 17055 0.0 26.4595 8 GCAGAGT 17645 0.0 25.278639 9 GAGTACT 10880 0.0 24.31846 12-13 AGTACTT 11215 0.0 23.147314 12-13 GTACATG 8980 0.0 23.076445 1 GTGGTAT 2840 0.0 22.593018 1 GTACTTT 11595 0.0 22.59156 14-15 CATGGGT 825 0.0 22.458351 4 GGACCGA 170 3.1503187E-6 22.3518 6 TACATGG 9220 0.0 22.311264 2 >>END_MODULE