FastQCFastQC Report
Thu 26 May 2016
SRR937577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047785
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103140.9843622498890517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.743568575614272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54340.5186178462184513No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18750.17894892559065076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15480.14774023296764124No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14850.1417275490677954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.13266080350453577No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA10590.10107035317359955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.041.144891
GTATCAA87650.038.9940641
ATCAACG112200.030.2823163
TCAACGC113300.029.9883144
TATCAAC113250.029.9624652
CAACGCA115000.029.5050985
AACGCAG117600.028.8835056
TAACACG856.3910795E-427.9529084
TACGGCC1205.3635213E-627.7199654
GTACATG96950.026.3298241
CTAAGGG4450.025.6311872
TACATGG98400.025.4043542
ACGCAGA133850.025.0574847
GGTAGTT3800.025.0033137
CGCAGAG134050.024.9137828
GCTAAGG4150.024.0542981
ACATGGG99500.023.831613
GAGTACT69550.023.69851512-13
GCAGAGT139550.023.4881959
AGTACTT71350.022.93422312-13
Produced by FastQC (version 0.11.5)