##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937577_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1047785 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90169548142033 33.0 31.0 34.0 30.0 34.0 2 32.01160257113816 33.0 31.0 34.0 30.0 34.0 3 32.01250829130022 34.0 31.0 34.0 30.0 34.0 4 35.61994684023917 37.0 35.0 37.0 33.0 37.0 5 35.63713738982711 37.0 35.0 37.0 33.0 37.0 6 35.711729982773186 37.0 35.0 37.0 35.0 37.0 7 35.665425635984484 37.0 35.0 37.0 33.0 37.0 8 35.651302509579736 37.0 35.0 37.0 33.0 37.0 9 37.274313909819284 39.0 37.0 39.0 34.0 39.0 10-11 37.17947670562186 39.0 37.0 39.0 34.0 39.0 12-13 37.0575027319536 39.0 37.0 39.0 33.0 39.0 14-15 38.18348563875223 40.0 38.0 41.0 33.0 41.0 16-17 38.14042814126944 40.0 38.0 41.0 33.0 41.0 18-19 38.15844805947785 40.0 38.0 41.0 33.5 41.0 20-21 38.130809278621086 40.0 38.0 41.0 34.0 41.0 22-23 37.947509269554345 40.0 38.0 41.0 33.0 41.0 24-25 37.78274502879884 40.0 37.5 41.0 33.0 41.0 26-27 37.71061715905458 40.0 37.0 41.0 33.0 41.0 28-29 37.738123756304965 40.0 37.0 41.0 33.0 41.0 30-31 37.50084177574598 40.0 37.0 41.0 33.0 41.0 32-33 37.21013423555405 39.0 36.0 41.0 32.0 41.0 34-35 37.18561727835386 39.5 36.0 41.0 32.0 41.0 36-37 37.074731934509465 40.0 36.0 41.0 32.0 41.0 38-39 36.92210902045744 40.0 36.0 41.0 31.0 41.0 40-41 36.656065891380386 39.0 35.0 41.0 30.5 41.0 42-43 36.34046631704024 39.0 35.0 41.0 30.0 41.0 44-45 35.98165511054272 39.0 35.0 41.0 29.5 41.0 46-47 35.46177030593108 39.0 35.0 41.0 26.5 41.0 48-49 34.973526534546686 39.0 35.0 40.5 24.0 41.0 50-51 33.99194729834842 38.0 34.0 39.5 22.5 40.5 52-53 34.05686090180714 38.0 34.0 39.5 23.0 40.5 54-55 34.39675792266543 38.0 34.0 40.0 22.5 41.0 56-57 34.20699857318057 38.0 34.0 40.0 21.5 41.0 58-59 33.97264419704425 38.0 34.0 40.0 20.0 41.0 60-61 33.6697332945213 38.0 34.0 40.0 18.5 41.0 62-63 33.324505981666086 37.0 33.0 40.0 16.5 41.0 64-65 32.95200542095945 37.0 33.0 40.0 12.5 41.0 66-67 32.557252680654905 36.0 32.5 39.0 8.0 41.0 68-69 32.086900938646764 36.0 32.0 39.0 7.0 40.5 70-71 31.656247703488788 35.0 31.0 38.5 3.5 40.0 72-73 31.22641954217707 35.0 31.0 38.0 2.0 40.0 74-75 30.777790290947095 35.0 31.0 37.0 2.0 39.0 76-77 30.298190945661563 35.0 30.0 36.5 2.0 39.0 78-79 29.792428313060405 34.5 29.5 36.0 2.0 39.0 80-81 29.289984109335407 34.0 29.0 36.0 2.0 37.5 82-83 28.7885992832499 34.0 29.0 35.0 2.0 37.0 84-85 28.34259509345906 34.0 28.5 35.0 2.0 36.5 86-87 27.8774896567521 34.0 27.0 35.0 2.0 36.0 88-89 27.41584103609042 33.5 26.0 35.0 2.0 36.0 90-91 26.948788635073036 33.0 25.5 35.0 2.0 35.0 92-93 26.420836335698638 33.0 25.0 35.0 2.0 35.0 94-95 25.826702997275206 32.5 23.5 35.0 2.0 35.0 96-97 25.140644311571553 32.0 19.0 34.5 2.0 35.0 98-99 24.264209737684734 32.0 4.5 34.0 2.0 35.0 100-101 21.98141985235521 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.07975120251131074 1101 2 0.09987585210088312 1101 3 -0.052108104489033025 1101 4 0.004450068453138556 1101 5 0.002431043745772854 1101 6 -0.04020073954995951 1101 7 -0.024369454154594905 1101 8 -0.175906364742616 1101 9 -0.04614298949429241 1101 10-11 0.09881004484266498 1101 12-13 0.14991835875276394 1101 14-15 0.31805710075992977 1101 16-17 0.37609416743030266 1101 18-19 0.3170972955617941 1101 20-21 0.3735066129216733 1101 22-23 0.2901471007770695 1101 24-25 0.32025823736619685 1101 26-27 0.38817600678151365 1101 28-29 0.180674486554409 1101 30-31 0.39283847101177116 1101 32-33 0.36202210144144686 1101 34-35 0.4630372303255825 1101 36-37 0.4705589310863658 1101 38-39 0.5162658907207387 1101 40-41 0.4400821647074409 1101 42-43 0.57057614028259 1101 44-45 0.6055071359065991 1101 46-47 0.5725232608795778 1101 48-49 0.6562076785893893 1101 50-51 0.6667837923508841 1101 52-53 0.7022349734617563 1101 54-55 0.7916132784944026 1101 56-57 0.6006056714263437 1101 58-59 0.7764127295703886 1101 60-61 0.825347317039764 1101 62-63 0.7758506743303641 1101 64-65 0.6619163867868707 1101 66-67 0.8293951081724913 1101 68-69 0.6654062077167602 1101 70-71 0.8705007473046784 1101 72-73 0.9384997891233553 1101 74-75 0.9705154254408193 1101 76-77 1.1680376470309675 1101 78-79 1.041611276617786 1101 80-81 0.9145392781953028 1101 82-83 0.9324888856211473 1101 84-85 1.2981824158957487 1101 86-87 1.184754948086983 1101 88-89 1.1509765382733796 1101 90-91 1.3512495246010623 1101 92-93 1.561280863331067 1101 94-95 1.723898927052904 1101 96-97 1.954437332490329 1101 98-99 2.1970429051963194 1101 100-101 2.4612944909595456 1102 1 0.3325807685063289 1102 2 0.036848858960684794 1102 3 0.23885962189046595 1102 4 0.21267580295830157 1102 5 0.1509282769187621 1102 6 0.2601139192080453 1102 7 0.12645577159196364 1102 8 0.35063588512778665 1102 9 0.2827453367863484 1102 10-11 0.16433200464831543 1102 12-13 0.3222524998308032 1102 14-15 0.33176886851524756 1102 16-17 0.369571327544584 1102 18-19 0.4246665867959578 1102 20-21 0.5239918625388924 1102 22-23 0.6135801363298867 1102 24-25 0.5072879069142218 1102 26-27 0.5162752749889208 1102 28-29 0.32445666071382107 1102 30-31 0.47078704058029075 1102 32-33 0.4119533828621158 1102 34-35 0.5465303042161409 1102 36-37 0.49623074561853286 1102 38-39 0.6104995580022532 1102 40-41 0.575334193111118 1102 42-43 0.7242134026308449 1102 44-45 0.8028216861615789 1102 46-47 0.8525683499123318 1102 48-49 0.9646492135454494 1102 50-51 0.8278083019775195 1102 52-53 0.7959182893140451 1102 54-55 1.0285537344482023 1102 56-57 1.2673904858258993 1102 58-59 1.1488397010099831 1102 60-61 1.154341735162177 1102 62-63 1.1750176122545781 1102 64-65 1.170682828008374 1102 66-67 1.2648852404256132 1102 68-69 1.0292397399723008 1102 70-71 1.1567606482052533 1102 72-73 1.1405589353251457 1102 74-75 1.3327732527281206 1102 76-77 1.2125998373452624 1102 78-79 1.1981859568352782 1102 80-81 1.4606349778589447 1102 82-83 1.1882829468891813 1102 84-85 1.1235817672888047 1102 86-87 1.3290265914254995 1102 88-89 1.2592474823702098 1102 90-91 1.3526787504922986 1102 92-93 1.6140525126975298 1102 94-95 1.7396390984876753 1102 96-97 1.8056423188068926 1102 98-99 1.808272967766392 1102 100-101 2.2268423709310916 1103 1 0.20636771085244376 1103 2 0.2750672124317326 1103 3 0.25605308625815226 1103 4 0.20533404333643546 1103 5 0.2315756534476563 1103 6 0.13729426776685472 1103 7 0.18879298398763922 1103 8 0.3775845416853798 1103 9 0.2867029702489816 1103 10-11 0.34745932079499653 1103 12-13 0.3244466138022304 1103 14-15 0.3782705976985383 1103 16-17 0.339218128710165 1103 18-19 0.32293833150787776 1103 20-21 0.38664366874662903 1103 22-23 0.4009722557084885 1103 24-25 0.43630910874301065 1103 26-27 0.2932666197923979 1103 28-29 0.22859803923482502 1103 30-31 0.18767673786494043 1103 32-33 0.3115283564139588 1103 34-35 0.4346453397587453 1103 36-37 0.4849545526676593 1103 38-39 0.5337185610770447 1103 40-41 0.5092803193074786 1103 42-43 0.40480633617946893 1103 44-45 0.35227880303457226 1103 46-47 0.31783063515642596 1103 48-49 0.40593709936366196 1103 50-51 0.36654169841521167 1103 52-53 0.36731902058225785 1103 54-55 0.5337211775460062 1103 56-57 0.4988439896647492 1103 58-59 0.455340069118769 1103 60-61 0.5848659963152585 1103 62-63 0.5022001261591882 1103 64-65 0.6703586448077488 1103 66-67 0.6881710598852635 1103 68-69 0.6473707990738724 1103 70-71 0.6915135203817933 1103 72-73 0.6111538486185211 1103 74-75 0.515344693672958 1103 76-77 0.7721852045569761 1103 78-79 0.7861475621607923 1103 80-81 0.5932515817305841 1103 82-83 0.8541462702619249 1103 84-85 0.9194413167731419 1103 86-87 0.5898539391243567 1103 88-89 0.8158619951364692 1103 90-91 1.1089625840205137 1103 92-93 1.246239140759176 1103 94-95 1.3072869761394479 1103 96-97 1.2836878776826453 1103 98-99 1.256487485482019 1103 100-101 2.0126964761174087 1104 1 0.2533194673713588 1104 2 0.25238508859405684 1104 3 0.23865644087818438 1104 4 0.2544948553841806 1104 5 0.14153665522368897 1104 6 0.1693735379524668 1104 7 0.15391109118146318 1104 8 0.22036320844442514 1104 9 0.27034223601115315 1104 10-11 0.2778474244047615 1104 12-13 0.30071725374883584 1104 14-15 0.5182741551040024 1104 16-17 0.5281126949398569 1104 18-19 0.40447919437189483 1104 20-21 0.5277041219894016 1104 22-23 0.5025598311701245 1104 24-25 0.4668341791250086 1104 26-27 0.3877943442386993 1104 28-29 0.39391480154284864 1104 30-31 0.3864274490129489 1104 32-33 0.24386703740262305 1104 34-35 0.3578340274758318 1104 36-37 0.5710880659586337 1104 38-39 0.54894412323371 1104 40-41 0.47225865377554044 1104 42-43 0.2803211273917512 1104 44-45 0.17788865824683597 1104 46-47 0.1445769948114517 1104 48-49 0.1144123847222076 1104 50-51 0.23909131889201518 1104 52-53 0.31013772480731205 1104 54-55 0.22661316165893197 1104 56-57 0.5105780982548822 1104 58-59 0.3210893160013768 1104 60-61 0.2520070947408044 1104 62-63 0.30810635188522895 1104 64-65 0.264344098083825 1104 66-67 0.35008796848659074 1104 68-69 0.35021785595371924 1104 70-71 0.34207337872511445 1104 72-73 0.4644275276819201 1104 74-75 0.591653039452769 1104 76-77 0.6073740734752455 1104 78-79 0.5782645115323817 1104 80-81 0.5934241225574723 1104 82-83 0.6328766495719478 1104 84-85 0.6489056274692082 1104 86-87 0.7290544155768615 1104 88-89 0.9295830545771189 1104 90-91 1.1483601833713841 1104 92-93 1.180513410058765 1104 94-95 1.3581175703594006 1104 96-97 1.5389970055587163 1104 98-99 1.4727447618417884 1104 100-101 1.8454774345383989 1105 1 0.20245650950039717 1105 2 0.08525144673998497 1105 3 0.1765277705452739 1105 4 0.02949209342798298 1105 5 0.13359756556445745 1105 6 0.11734288637630641 1105 7 0.2243026138172297 1105 8 0.1716530419905311 1105 9 0.22254811836409516 1105 10-11 0.2783553174618163 1105 12-13 0.12611635641994923 1105 14-15 0.26721617243681095 1105 16-17 0.2862650689946591 1105 18-19 0.2664657774024306 1105 20-21 0.2997606438886464 1105 22-23 0.2838819489153863 1105 24-25 0.34780460778061695 1105 26-27 0.3008333860240313 1105 28-29 0.36384477060893516 1105 30-31 0.16314553921230157 1105 32-33 0.27827947725335633 1105 34-35 0.1753991482653845 1105 36-37 0.17894705888802775 1105 38-39 0.11434417724529311 1105 40-41 0.2757730895999515 1105 42-43 0.2661116299450228 1105 44-45 0.18705988725597678 1105 46-47 0.5295277092374562 1105 48-49 0.46040417250576837 1105 50-51 0.25343814693875544 1105 52-53 0.5152881593550873 1105 54-55 0.5550941471249047 1105 56-57 0.4149031682244413 1105 58-59 0.475563224721796 1105 60-61 0.5809184850481728 1105 62-63 0.6188609490078889 1105 64-65 0.7230091218980235 1105 66-67 0.6311208480407515 1105 68-69 0.8303049189508691 1105 70-71 0.8193739347989641 1105 72-73 0.8943942921422945 1105 74-75 0.9020654608904586 1105 76-77 0.9938476998623607 1105 78-79 0.8990779996989318 1105 80-81 1.1071487432488212 1105 82-83 1.1462023210341883 1105 84-85 1.2036276519217246 1105 86-87 1.3580400718176584 1105 88-89 1.103282480090229 1105 90-91 1.1931491392291882 1105 92-93 1.1899723428388889 1105 94-95 1.1171998030020376 1105 96-97 0.9675858607585575 1105 98-99 1.2415322016925217 1105 100-101 1.5632615170779225 1106 1 0.15293796850050612 1106 2 0.19534035800805682 1106 3 0.2019630149995315 1106 4 0.14646145387384735 1106 5 0.2098619127667547 1106 6 0.02703655981191133 1106 7 0.10879211552785506 1106 8 0.04156771752303001 1106 9 0.23188069754961305 1106 10-11 0.0197452716611366 1106 12-13 0.05131863127923708 1106 14-15 0.020355485150645336 1106 16-17 0.03115560260022221 1106 18-19 0.263118671674583 1106 20-21 0.4791084324881325 1106 22-23 0.3257250815718251 1106 24-25 0.1905630643527374 1106 26-27 0.14273221755477294 1106 28-29 0.48898100005381195 1106 30-31 0.560332832281496 1106 32-33 0.5007421607319955 1106 34-35 0.3754822654709926 1106 36-37 0.4434339394751632 1106 38-39 0.43523522128017333 1106 40-41 0.6022478164512961 1106 42-43 0.4828052550967712 1106 44-45 0.6196090678099964 1106 46-47 0.7088280761947487 1106 48-49 0.726163677527289 1106 50-51 0.6103213551939106 1106 52-53 0.6409486134992193 1106 54-55 0.7766739258469002 1106 56-57 0.7967758574971526 1106 58-59 0.7945237768131719 1106 60-61 0.7712629213686739 1106 62-63 0.9096271792804842 1106 64-65 0.8579961197459411 1106 66-67 0.9006663245334181 1106 68-69 0.9444970955869998 1106 70-71 0.8844407389596505 1106 72-73 0.7601729382630324 1106 74-75 0.8239930558027204 1106 76-77 0.9750851115718895 1106 78-79 1.0524240707727657 1106 80-81 1.0149239957044003 1106 82-83 0.7180530066649133 1106 84-85 0.738535153853082 1106 86-87 1.0688327202560188 1106 88-89 1.2022678293894238 1106 90-91 1.2683193398141093 1106 92-93 1.4570139566232392 1106 94-95 1.4853762597948865 1106 96-97 1.684743355792218 1106 98-99 2.055604875790305 1106 100-101 2.5305983521402773 1107 1 0.09041456157368444 1107 2 -0.03997456579617875 1107 3 0.15648799233891353 1107 4 0.08034491406443323 1107 5 0.0986710949762184 1107 6 0.09526899534448319 1107 7 0.168115897327354 1107 8 0.2663221596375891 1107 9 0.187892862819254 1107 10-11 0.243263104347065 1107 12-13 0.2419025786957434 1107 14-15 0.5372333493895596 1107 16-17 0.33527965433699336 1107 18-19 0.35522843410445404 1107 20-21 0.3857132113910495 1107 22-23 0.3422383857524167 1107 24-25 0.4267432529108035 1107 26-27 0.47996338843483244 1107 28-29 0.3823928660766285 1107 30-31 0.578021684342481 1107 32-33 0.29453731235461333 1107 34-35 0.41414372396452137 1107 36-37 0.5836149711348924 1107 38-39 0.5597094135037324 1107 40-41 0.6137070336693853 1107 42-43 0.6114813864541802 1107 44-45 0.4495508467545264 1107 46-47 0.42925348069358193 1107 48-49 0.8745043412618045 1107 50-51 0.7972845789541836 1107 52-53 0.7083782027494934 1107 54-55 0.8061639397960718 1107 56-57 0.9427102439235711 1107 58-59 0.8592797748664438 1107 60-61 0.9993354932448995 1107 62-63 1.0136973159317009 1107 64-65 1.1970252139905995 1107 66-67 1.0659463663628372 1107 68-69 1.0412574864532793 1107 70-71 1.1514692216842128 1107 72-73 1.3125551527980335 1107 74-75 1.3738816976310595 1107 76-77 1.502458777490137 1107 78-79 1.60227833721099 1107 80-81 1.3165644255053266 1107 82-83 1.4192028634642782 1107 84-85 1.4704279171097703 1107 86-87 1.582658864006664 1107 88-89 1.7502508986051772 1107 90-91 1.7676956074066226 1107 92-93 1.7200847464068723 1107 94-95 1.7610492485179527 1107 96-97 1.955991417653447 1107 98-99 2.26955775617661 1107 100-101 2.7933498126420524 1108 1 0.06559341390465079 1108 2 0.10742950207856339 1108 3 0.17390091541098585 1108 4 0.12430766904535773 1108 5 0.08124635184096718 1108 6 0.08553615832246919 1108 7 0.1706473608580268 1108 8 0.20930118986050417 1108 9 0.29128197043403503 1108 10-11 0.19121000862327264 1108 12-13 0.301989377359682 1108 14-15 0.20167012830415132 1108 16-17 0.1931882123582298 1108 18-19 0.27287706893640973 1108 20-21 0.42645007412958336 1108 22-23 0.2740119960823577 1108 24-25 0.3414890772272301 1108 26-27 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-0.3061924839093564 2316 70-71 -0.40701148748921767 2316 72-73 -0.3446732672208981 2316 74-75 -0.48172518266053643 2316 76-77 -0.7639816646816193 2316 78-79 -0.8328938027835235 2316 80-81 -0.7287718922071988 2316 82-83 -0.5206006673630057 2316 84-85 -0.41935703507233413 2316 86-87 -0.49406209358979325 2316 88-89 -0.5480430730668147 2316 90-91 -0.707320538320733 2316 92-93 -0.6497934086451913 2316 94-95 -0.589230647618578 2316 96-97 -0.3467481583995671 2316 98-99 0.1616279923023427 2316 100-101 0.3160821120590356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3945.0 3 616.0 4 616.0 5 683.0 6 727.0 7 898.0 8 1154.0 9 1392.0 10 1846.0 11 2528.0 12 3794.0 13 5616.0 14 6296.0 15 8527.0 16 11909.0 17 17605.0 18 14382.0 19 7824.0 20 7120.0 21 7222.0 22 7545.0 23 8483.0 24 9352.0 25 10385.0 26 11937.0 27 13886.0 28 16308.0 29 19965.0 30 24323.0 31 30286.0 32 38907.0 33 51118.0 34 70203.0 35 102588.0 36 146265.0 37 200968.0 38 160993.0 39 19571.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.92172729335249 19.752215405263364 14.969888485444905 20.356168815939238 2 14.983245388773023 21.077789061311062 41.28879857808338 22.650166971832537 3 19.082682244314377 27.31396590730738 30.21869700530952 23.384654843068724 4 11.935544062274234 17.164455459282046 37.823272459344246 33.07672801909947 5 12.412782798069452 39.67784654365357 32.98551706452638 14.923853593750597 6 29.711339160942835 37.12217224000773 17.780298675349098 15.38618992370034 7 27.5200341242417 35.444165070280825 19.97081076635128 17.064990039126197 8 27.38133384346828 34.412874530715705 19.301752791793202 18.904038834022813 9 27.997937684792912 16.63502090533342 20.470965452394093 34.896075957479574 10-11 24.604138727925605 28.32830515705319 26.03449067475766 21.03306544026354 12-13 26.210735738608104 26.139763553583045 27.49253931208632 20.15696139572254 14-15 23.09355813991302 26.384309832131542 27.27362721552221 23.248504812433225 16-17 20.314677455608003 30.5023361692563 27.57310990277519 21.60987647236051 18-19 20.391790027632474 27.552943111919646 31.989633757337337 20.065633103110542 20-21 21.76878099722854 26.41499419504274 32.07951356944622 19.7367112382825 22-23 21.3010876742208 26.834556761638744 32.006710886352415 19.85764467778805 24-25 20.56699948984007 27.002568968410724 32.46209301358211 19.96833852816709 26-27 20.40306988830553 27.292051123998217 32.09356952223919 20.211309465457063 28-29 20.105975734340383 27.328615648327766 32.44336290973649 20.12204570759536 30-31 20.265012843180248 27.179048423404573 32.44582836958326 20.11011036383192 32-33 19.95383775107777 27.363615232968225 32.09830665086476 20.584240365089247 34-35 20.409047499035175 27.348661176928562 32.090339173612534 20.151952150423732 36-37 20.737764138678276 26.974002246371164 31.84214389303104 20.446089721919524 38-39 20.389569963990336 27.278456408988696 31.951488560440627 20.380485066580345 40-41 20.333388158523853 27.379923270233363 31.955212919364897 20.331475651877888 42-43 20.46614606994104 27.478325737976945 31.55213060506219 20.50339758701983 44-45 20.35988502729669 27.8061416004273 31.15978308011509 20.674190292160922 46-47 20.754278500624025 27.977688199036947 30.591650049969637 20.676383250369394 48-49 20.83079673255532 28.43423863479309 29.79226255862477 20.942702074026815 50-51 20.980118982882402 28.575739792978013 29.511800961217933 20.932340262921656 52-53 21.045013570176035 28.836169661161744 29.348794181996297 20.770022586665927 54-55 20.796656750220812 28.916585650212635 29.201789072844992 21.08496852672156 56-57 20.97548569437121 29.112835411050487 29.01397313986669 20.897705754711616 58-59 21.01466907924458 28.636853641454906 29.18929255216164 21.15918472713887 60-61 20.782060138980853 28.68030339611456 29.651687300247893 20.885949164656694 62-63 21.01077512426583 28.725154005481272 29.73811896242094 20.525951907831953 64-65 20.827134383043497 28.765073488635363 29.657483296298988 20.750308832022153 66-67 20.691669794248917 28.931275264689077 29.40913795864898 20.967916982413026 68-69 20.689327082052365 28.97010687930967 29.4084009795756 20.932165059062367 70-71 20.65028080285665 29.104342392554123 29.34969529292469 20.89568151166454 72-73 20.7463241727297 29.24655222366394 28.87320846150242 21.13391514210394 74-75 20.55431061149729 29.56729654408098 28.880779089019686 20.99761375540204 76-77 20.69694865585536 29.414951815848166 29.0393679249253 20.848731603371167 78-79 20.729453251990723 29.357511669846055 28.721713684792448 21.19132139337077 80-81 20.550930833831828 29.62429535355332 28.71619860784958 21.108575204765273 82-83 20.68941444241491 29.444805722646723 28.883538783746303 20.98224105119206 84-85 20.620939891630037 29.272798642530688 28.7628870415517 21.34337442428757 86-87 20.800465889737517 29.226213756561137 28.676842545142634 21.296477808558716 88-89 20.847939444509528 29.08777055474931 28.66989750418997 21.394392496551195 90-91 20.51708605572812 29.133513381176073 28.99795150949459 21.35144905360121 92-93 20.64673179441105 29.323550942752075 28.705871777302704 21.32384548553417 94-95 20.323781254004473 29.129363320091876 28.738240002448105 21.808615423455542 96-97 20.701688458471263 29.092059139075506 28.4777004530477 21.72855194940553 98-99 20.57431183799774 29.35824778374326 28.271169130714803 21.7962712475442 100-101 20.719838219264528 28.88173369284608 28.87207656856558 21.52635151932381 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2271.0 1 1358.0 2 532.5 3 663.5 4 653.0 5 682.5 6 1146.5 7 2262.5 8 3575.5 9 4128.5 10 3647.5 11 3115.0 12 3083.0 13 3266.5 14 3396.5 15 3385.0 16 3386.5 17 3455.0 18 3559.0 19 3721.0 20 3946.0 21 4280.0 22 4894.5 23 5680.0 24 6370.0 25 7428.0 26 9004.0 27 10457.0 28 11397.0 29 12605.5 30 14921.0 31 17162.5 32 19785.5 33 22473.5 34 24892.0 35 26800.0 36 29322.0 37 32185.5 38 32524.5 39 32059.0 40 32768.0 41 34000.0 42 34727.5 43 36258.5 44 38294.0 45 39953.5 46 39043.0 47 37173.5 48 37757.5 49 36413.5 50 33175.0 51 30043.0 52 27621.5 53 26541.5 54 25486.5 55 23886.0 56 21668.0 57 19575.0 58 17934.0 59 16082.5 60 13864.5 61 11645.5 62 9409.5 63 6974.5 64 4893.5 65 3700.5 66 2770.0 67 2030.5 68 1431.5 69 964.5 70 719.0 71 559.5 72 447.5 73 414.5 74 336.0 75 245.0 76 190.5 77 124.0 78 76.0 79 60.5 80 50.0 81 37.5 82 26.5 83 15.5 84 9.0 85 7.0 86 5.5 87 5.5 88 6.0 89 4.5 90 4.0 91 3.0 92 2.0 93 2.5 94 3.0 95 1.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30235210467796353 2 0.28612740209107784 3 0.2738157160104411 4 0.2605496356599875 5 0.2570183768616653 6 0.21970156091182827 7 0.2093941027978068 8 0.22046507632768175 9 0.2250461688228024 10-11 0.22361457741807717 12-13 0.21998787919277332 14-15 0.21607486268652443 16-17 0.2166474992484145 18-19 0.21722013581030458 20-21 0.2029996611900342 22-23 0.19293080164346693 24-25 0.19417151419422876 26-27 0.21087341391602285 28-29 0.22495072939582073 30-31 0.21841312864757562 32-33 0.22351913799109552 34-35 0.21554994583812517 36-37 0.20027963752105635 38-39 0.19980244038614792 40-41 0.194410112761683 42-43 0.20901234508988006 44-45 0.20476529058919532 46-47 0.20519476801061287 48-49 0.21554994583812517 50-51 0.223375978850623 52-53 0.23649890006060403 54-55 0.21144605047791293 56-57 0.1799510395739584 58-59 0.17918752415810496 60-61 0.1868226783166394 62-63 0.19498274932357307 64-65 0.18219386610802787 66-67 0.18920866399118139 68-69 0.1933602790648845 70-71 0.18558196576587754 72-73 0.18763391344598368 74-75 0.19083113424986994 76-77 0.21120745191045873 78-79 0.21459555156830837 80-81 0.2039063357463602 82-83 0.20185438806625405 84-85 0.19235816508157685 86-87 0.19507818875055474 88-89 0.20352457803843346 90-91 0.203954055459851 92-93 0.20008875866709294 94-95 0.19841856869491353 96-97 0.1888746259967455 98-99 0.19531678731800894 100-101 0.18372089693973476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1047785.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.943897362645444 #Duplication Level Percentage of deduplicated Percentage of total 1 76.62739841863008 39.8032571862386 2 12.7267492294261 13.221539114668722 3 4.301375582760078 6.702906353672361 4 1.9619796388316437 4.076514759482842 5 1.0780871283171978 2.8000023570648844 6 0.6669874765313003 2.078755741386705 7 0.4523979379366838 1.644951843867888 8 0.32569711809694674 1.3534382138989771 9 0.23823812845485298 1.113751520309486 >10 1.43625586400797 13.854866435345157 >50 0.12095297764617888 4.361429341443025 >100 0.060557236630319144 5.409148801621738 >500 0.0018462570726051218 0.6164566925213003 >1k 9.231285363025609E-4 0.7055494534659624 >5k 3.6925141452102435E-4 1.2683011447719659 >10k+ 1.8462570726051217E-4 0.9891310402403067 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10314 0.9843622498890517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7791 0.743568575614272 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5434 0.5186178462184513 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1875 0.17894892559065076 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1548 0.14774023296764124 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1485 0.1417275490677954 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 0.13266080350453577 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1059 0.10107035317359955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.54394269816804E-5 0.0 0.0 0.0 0.0 7 9.54394269816804E-5 0.0 0.0 0.0 0.0 8 9.54394269816804E-5 0.0 0.0 0.0 0.0 9 9.54394269816804E-5 0.0 0.0 0.0 0.0 10-11 9.54394269816804E-5 0.0 0.0 0.0 0.0 12-13 2.863182809450412E-4 0.0 0.0 0.0 0.0 14-15 2.863182809450412E-4 0.0 0.0 0.0 0.0 16-17 2.863182809450412E-4 0.0 0.0 0.0 0.0 18-19 3.3403799443588143E-4 0.0 0.0 0.0 0.0 20-21 3.817577079267216E-4 0.0 0.0 4.77197134908402E-5 0.0 22-23 3.817577079267216E-4 0.0 0.0 1.908788539633608E-4 0.0 24-25 4.77197134908402E-4 0.0 0.0 7.15795702362603E-4 0.0 26-27 4.77197134908402E-4 0.0 0.0 0.0011452731237801648 0.0 28-29 6.680759888717629E-4 0.0 0.0 0.00243370538803285 0.0 30-31 6.680759888717629E-4 0.0 0.0 0.005153729057010742 0.0 32-33 6.680759888717629E-4 0.0 0.0 0.008064631579951994 0.0 34-35 8.589548428351236E-4 0.0 0.0 0.010546056681475685 0.0 36-37 9.54394269816804E-4 0.0 0.0 0.015699785738486426 0.0 38-39 0.0010498336967984844 0.0 0.0 0.02347809903749338 0.0 40-41 0.0010498336967984844 0.0 0.0 0.03278344316820722 0.0 42-43 0.0012407125507618451 0.0 0.0 0.04600180380516995 0.0 44-45 0.0012407125507618451 0.0 0.0 0.05540258736286548 0.0 46-47 0.0013361519777435257 0.0 0.0 0.07305888135447636 0.0 48-49 0.0013361519777435257 0.0 0.0 0.09792085208320409 0.0 50-51 0.0013361519777435257 0.0 0.0 0.11276168297885539 0.0 52-53 0.0013361519777435257 0.0 0.0 0.14062999565750606 0.0 54-55 0.0013361519777435257 0.0 0.0 0.18085771413028437 0.0 56-57 0.0013361519777435257 0.0 0.0 0.2426070233874316 0.0 58-59 0.0013361519777435257 0.0 0.0 0.30478581006599637 0.0 60-61 0.0013361519777435257 0.0 0.0 0.3978392513731348 0.0 62-63 0.0013361519777435257 0.0 0.0 0.48597756219071664 0.0 64-65 0.0013361519777435257 0.0 0.0 0.5684372271028885 0.0 66-67 0.0014315914047252061 0.0 0.0 0.6866866771331905 0.0 68-69 0.0014315914047252061 0.0 0.0 0.8467863158949593 0.0 70-71 0.0015747505451977266 0.0 0.0 0.9489542224788483 0.0 72-73 0.0017656293991610873 0.0 0.0 1.075936380077974 0.0 74-75 0.0018133491126519276 0.0 0.0 1.225251363590813 0.0 76-77 0.0018133491126519276 0.0 0.0 1.3791951593122636 0.0 78-79 0.0018133491126519276 0.0 0.0 1.5515587644411784 0.0 80-81 0.0020042279666152886 0.0 0.0 1.814160347781272 0.0 82-83 0.0020042279666152886 0.0 0.0 2.10596639577776 0.0 84-85 0.0020996673935969688 0.0 0.0 2.4455875966920697 0.0 86-87 0.0021951068205786494 0.0 0.0 2.8750650181096313 0.0 88-89 0.0021951068205786494 0.0 0.0 3.346249469118187 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5095 0.0 41.14489 1 GTATCAA 8765 0.0 38.994064 1 ATCAACG 11220 0.0 30.282316 3 TCAACGC 11330 0.0 29.988314 4 TATCAAC 11325 0.0 29.962465 2 CAACGCA 11500 0.0 29.505098 5 AACGCAG 11760 0.0 28.883505 6 TAACACG 85 6.3910795E-4 27.952908 4 TACGGCC 120 5.3635213E-6 27.719965 4 GTACATG 9695 0.0 26.329824 1 CTAAGGG 445 0.0 25.631187 2 TACATGG 9840 0.0 25.404354 2 ACGCAGA 13385 0.0 25.057484 7 GGTAGTT 380 0.0 25.003313 7 CGCAGAG 13405 0.0 24.913782 8 GCTAAGG 415 0.0 24.054298 1 ACATGGG 9950 0.0 23.83161 3 GAGTACT 6955 0.0 23.698515 12-13 GCAGAGT 13955 0.0 23.488195 9 AGTACTT 7135 0.0 22.934223 12-13 >>END_MODULE