FastQCFastQC Report
Thu 26 May 2016
SRR937577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047785
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152721.457550928864223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124801.1910840487313714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91190.8703121346459435No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.2779196113706533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.2429887810953583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.11767681346841194No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.10985078045591413No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.10965990160195077No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA10540.10059315603869114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.050.9308661
GTATCAA102300.039.2998051
TATCAAC134500.029.7710152
ATCAACG135500.029.5513063
TCAACGC136900.029.2837964
CAACGCA139300.028.7451695
AACGCAG142200.028.1910026
ACGCAGA161300.024.7668487
CGCAGAG162050.024.6229088
GTACATG86800.023.8158041
GCAGAGT168300.023.4544939
TACATGG88150.023.3321062
GAGTACT106550.023.22701512-13
ACATGGG88850.022.6136823
AGTACTT108800.022.2664212-13
GTACTTT112150.021.91688214-15
CGCGGAA550.00287914621.58961982-83
CATGGGA66450.021.1585124
ATGGGAT23900.021.0666925
CATATAG2509.487849E-920.8997483
Produced by FastQC (version 0.11.5)