##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1047785 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.302343515129536 33.0 31.0 34.0 31.0 34.0 2 32.491295447062136 34.0 31.0 34.0 31.0 34.0 3 32.524229684524975 34.0 31.0 34.0 31.0 34.0 4 36.13635240054019 37.0 35.0 37.0 35.0 37.0 5 36.083257538521735 37.0 35.0 37.0 35.0 37.0 6 36.15839222741307 37.0 35.0 37.0 35.0 37.0 7 36.1085451690948 37.0 35.0 37.0 35.0 37.0 8 36.08668572273892 37.0 35.0 37.0 35.0 37.0 9 37.7619769322905 39.0 37.0 39.0 35.0 39.0 10-11 37.70165730564953 39.0 37.0 39.0 35.0 39.0 12-13 37.58803666782785 39.0 37.0 39.0 35.0 39.0 14-15 38.74372605066879 40.0 38.0 41.0 34.5 41.0 16-17 38.72739636471223 40.0 38.0 41.0 34.5 41.0 18-19 38.8139269983823 40.0 38.0 41.0 35.0 41.0 20-21 38.76516079157461 40.0 38.0 41.0 35.0 41.0 22-23 38.7028393229527 40.0 38.0 41.0 34.5 41.0 24-25 38.69350009782541 40.0 38.0 41.0 34.5 41.0 26-27 38.5024947866213 40.0 38.0 41.0 34.0 41.0 28-29 38.428709134030356 40.0 38.0 41.0 34.0 41.0 30-31 38.304901291772644 40.0 37.5 41.0 34.0 41.0 32-33 38.04499348625911 40.0 37.0 41.0 34.0 41.0 34-35 37.84475727367733 40.0 36.0 41.0 33.0 41.0 36-37 37.629439245646765 40.0 36.0 41.0 33.0 41.0 38-39 37.44703684439078 40.0 36.0 41.0 33.0 41.0 40-41 37.1957362435996 40.0 35.5 41.0 33.0 41.0 42-43 36.86439584456735 40.0 35.0 41.0 31.5 41.0 44-45 36.35914190411201 39.0 35.0 41.0 30.5 41.0 46-47 35.70704199811985 39.0 35.0 41.0 28.0 41.0 48-49 35.12637086806931 39.0 35.0 41.0 24.5 41.0 50-51 34.798467719999806 39.0 35.0 41.0 23.5 41.0 52-53 34.51179965355488 39.0 35.0 40.0 22.5 41.0 54-55 34.26509302958145 38.0 34.0 40.0 20.5 41.0 56-57 34.00380087517954 38.0 34.0 40.0 19.5 41.0 58-59 33.743648267535804 38.0 34.0 40.0 18.0 41.0 60-61 33.409914724871996 37.5 33.5 40.0 14.0 41.0 62-63 33.008127144404625 37.0 33.0 40.0 9.5 41.0 64-65 32.60940698712045 36.5 33.0 39.5 7.5 41.0 66-67 32.188949545946926 36.0 32.0 39.0 4.0 40.5 68-69 31.723571152478797 35.5 31.5 39.0 2.0 40.0 70-71 31.296790372070603 35.0 31.0 38.0 2.0 40.0 72-73 30.840788425106297 35.0 31.0 37.5 2.0 39.5 74-75 30.362728994975114 35.0 30.0 37.0 2.0 39.0 76-77 29.11307042952514 33.5 28.5 35.5 2.0 38.0 78-79 29.6897393072052 34.0 30.0 36.0 2.0 38.0 80-81 29.50631045491203 34.0 30.0 36.0 2.0 37.0 82-83 29.115128580768 34.0 29.5 35.0 2.0 37.0 84-85 28.72348143941744 34.0 29.0 35.0 2.0 36.5 86-87 28.38281422238341 34.0 29.0 35.0 2.0 36.0 88-89 27.9853567287182 34.0 28.5 35.0 2.0 36.0 90-91 27.494113296143773 34.0 27.0 35.0 2.0 35.0 92-93 27.052048368701595 33.0 26.0 35.0 2.0 35.0 94-95 26.592500847024915 33.0 25.0 35.0 2.0 35.0 96-97 26.11226348917001 33.0 24.5 35.0 2.0 35.0 98-99 25.34962229846772 32.5 21.0 34.5 2.0 35.0 100-101 23.0133309791608 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10456312811069068 1101 2 0.20849321092547513 1101 3 0.12219510062024597 1101 4 0.04270276174477772 1101 5 0.08278052858381812 1101 6 0.1076249523986661 1101 7 0.1055248777831963 1101 8 0.0924064936934812 1101 9 0.21721647571617808 1101 10-11 0.15903146924958378 1101 12-13 0.23445665410148564 1101 14-15 0.23772727917013725 1101 16-17 0.30764332293993135 1101 18-19 0.23583961649064378 1101 20-21 0.23700723781093558 1101 22-23 0.21972796272383732 1101 24-25 0.20801619472679533 1101 26-27 0.2548921438084122 1101 28-29 0.22836415178539937 1101 30-31 0.31070102273556444 1101 32-33 0.3642001939325965 1101 34-35 0.26668037953381685 1101 36-37 0.4544919250680479 1101 38-39 0.5091240808248969 1101 40-41 0.4901690842309918 1101 42-43 0.4545652011890624 1101 44-45 0.61414352076077 1101 46-47 0.6501820730348342 1101 48-49 0.3687984761923744 1101 50-51 0.4799714637543886 1101 52-53 0.6673145876005222 1101 54-55 0.7870016026071767 1101 56-57 0.8253782719737686 1101 58-59 0.7475195667367132 1101 60-61 0.9099780139415046 1101 62-63 0.7232822870896172 1101 64-65 0.9151099940358023 1101 66-67 0.9325274178211913 1101 68-69 1.0152909156173777 1101 70-71 0.8939854461801424 1101 72-73 0.8558723011586444 1101 74-75 0.793011118238752 1101 76-77 0.6539775633544984 1101 78-79 0.8954540897353951 1101 80-81 0.9731577108196845 1101 82-83 0.852688056056266 1101 84-85 0.9114133595000204 1101 86-87 1.1002058484748503 1101 88-89 1.2425316210307678 1101 90-91 1.197087118977091 1101 92-93 1.4023308863161077 1101 94-95 1.2402978134025737 1101 96-97 1.395320704714159 1101 98-99 1.3443890816138726 1101 100-101 1.6406302598769678 1102 1 0.20883694150960963 1102 2 0.17851503208839148 1102 3 0.22264237418047372 1102 4 0.19337608326333822 1102 5 0.1280684835430108 1102 6 0.21846681527496514 1102 7 0.14130796819032554 1102 8 0.1694291708246638 1102 9 0.18092221552593202 1102 10-11 0.20836538126569337 1102 12-13 0.2338363785038169 1102 14-15 0.29053208223715643 1102 16-17 0.30296684277790575 1102 18-19 0.42062689029199163 1102 20-21 0.27192230405805873 1102 22-23 0.2668996506691528 1102 24-25 0.29247434270080674 1102 26-27 0.2746644080253233 1102 28-29 0.4097046463180547 1102 30-31 0.47289073423809214 1102 32-33 0.4704879439416345 1102 34-35 0.45295535029828216 1102 36-37 0.5544207953104845 1102 38-39 0.6086888631557841 1102 40-41 0.5553269561250929 1102 42-43 0.5270168872054413 1102 44-45 0.7148867664477692 1102 46-47 0.7660409949970557 1102 48-49 0.6920247532126425 1102 50-51 0.6298286014447214 1102 52-53 0.7453644266543762 1102 54-55 0.7507386877338291 1102 56-57 0.8487028045065941 1102 58-59 0.8083692659422326 1102 60-61 0.9469178671007441 1102 62-63 1.089914594466613 1102 64-65 1.0788916859595687 1102 66-67 1.1456044429095016 1102 68-69 1.0578717202275527 1102 70-71 1.1034270173038756 1102 72-73 1.0916125931523268 1102 74-75 1.2470504445555584 1102 76-77 0.9794498528887807 1102 78-79 0.909836165036495 1102 80-81 0.9669085396621959 1102 82-83 1.2526066787912669 1102 84-85 1.394550181808242 1102 86-87 1.2291472206763778 1102 88-89 1.3245593013567607 1102 90-91 1.268360944431894 1102 92-93 1.466474857767757 1102 94-95 1.5307941544396222 1102 96-97 1.5445852953563772 1102 98-99 1.5290045589668786 1102 100-101 1.7616786401679967 1103 1 0.2643922908417551 1103 2 0.28673963412876446 1103 3 0.34724688923581226 1103 4 0.25675637366714454 1103 5 0.21747561186140274 1103 6 0.18852317613293224 1103 7 0.15255477430525843 1103 8 0.2075590544527941 1103 9 0.12185467529690186 1103 10-11 0.2685397225365591 1103 12-13 0.3447729214983042 1103 14-15 0.41891234170746117 1103 16-17 0.3956476646194389 1103 18-19 0.42620389308218876 1103 20-21 0.3991512182299388 1103 22-23 0.3119867903710798 1103 24-25 0.2190131302308842 1103 26-27 0.2626325093882542 1103 28-29 0.21616047017026574 1103 30-31 0.4058893669768793 1103 32-33 0.3390267067249084 1103 34-35 0.5018708213847347 1103 36-37 0.34695561097769456 1103 38-39 0.378941961146964 1103 40-41 0.5669307349628525 1103 42-43 0.7558883708385196 1103 44-45 0.606334269185389 1103 46-47 0.7157019637108988 1103 48-49 0.7651985933606298 1103 50-51 0.7167195979368586 1103 52-53 0.5732240751259496 1103 54-55 0.5684685391911017 1103 56-57 0.6617858238647827 1103 58-59 0.5762562596379865 1103 60-61 0.813403882060193 1103 62-63 0.6749907218384479 1103 64-65 0.8458389195756268 1103 66-67 0.930993308082698 1103 68-69 0.9783466114962565 1103 70-71 1.0245864671823064 1103 72-73 0.9654165489297775 1103 74-75 1.013989944793746 1103 76-77 0.8277893419909041 1103 78-79 0.7235177478424077 1103 80-81 0.9144041625703139 1103 82-83 0.9562323956753964 1103 84-85 0.9725397510281617 1103 86-87 1.0397441137144803 1103 88-89 1.0969972332659133 1103 90-91 1.1628439526540255 1103 92-93 1.2383027808299296 1103 94-95 1.3272033924234954 1103 96-97 1.445661162886708 1103 98-99 1.5391055878154702 1103 100-101 1.940704172688399 1104 1 0.16442053690762037 1104 2 0.12233354097270421 1104 3 0.10742579875113734 1104 4 0.12608434380092604 1104 5 0.13681044365741712 1104 6 0.0961958468650792 1104 7 0.11009574109348108 1104 8 0.1909844761785493 1104 9 0.20305212195913924 1104 10-11 0.14726483901827692 1104 12-13 0.1944762045705133 1104 14-15 0.2472428793020356 1104 16-17 0.11291753579339314 1104 18-19 0.2506452671733541 1104 20-21 0.1753297919901371 1104 22-23 0.12938363560527932 1104 24-25 0.1550760949752643 1104 26-27 0.014377236900322998 1104 28-29 0.20086562521051832 1104 30-31 0.1513153696122913 1104 32-33 0.2599044132509505 1104 34-35 0.2483300704390885 1104 36-37 0.2838063345166617 1104 38-39 0.2452271504822079 1104 40-41 0.30387492366789104 1104 42-43 0.463180519098394 1104 44-45 0.49511738336744315 1104 46-47 0.5324223023439174 1104 48-49 0.6094814963633723 1104 50-51 0.6615694874174594 1104 52-53 0.6313759963247563 1104 54-55 0.587620570975929 1104 56-57 0.6046351724065815 1104 58-59 0.8005959745392488 1104 60-61 0.7247918431072691 1104 62-63 0.4991379771069333 1104 64-65 0.6625019666599599 1104 66-67 0.631504068901144 1104 68-69 0.6772748598271363 1104 70-71 0.6790841202273477 1104 72-73 0.5688860851480264 1104 74-75 0.644316559915783 1104 76-77 0.48544369275108323 1104 78-79 0.46960826582462545 1104 80-81 0.37537096411639226 1104 82-83 0.4781550734024407 1104 84-85 0.41147761900977997 1104 86-87 0.4429803534169885 1104 88-89 0.6557657047940104 1104 90-91 0.5633215376686884 1104 92-93 0.6599219465850368 1104 94-95 0.6110940082010572 1104 96-97 0.9770159614536169 1104 98-99 1.1274548184432653 1104 100-101 1.1870801491429006 1105 1 0.19610324337919138 1105 2 0.19402318302318378 1105 3 0.2691981767340508 1105 4 0.14679748089216105 1105 5 0.17711659115815337 1105 6 0.16386837706247093 1105 7 0.20531418114062205 1105 8 0.1702713390873143 1105 9 0.22831021142197017 1105 10-11 0.1818100062086927 1105 12-13 0.26235895032324663 1105 14-15 0.3408056800483834 1105 16-17 0.3969049412731138 1105 18-19 0.2796435056590596 1105 20-21 0.1726675730959002 1105 22-23 0.32552011920540735 1105 24-25 0.26102626928538797 1105 26-27 0.19210911846764134 1105 28-29 0.11738319738964265 1105 30-31 -0.021903782405779282 1105 32-33 0.019756924789788854 1105 34-35 0.0664613922499484 1105 36-37 -0.0463885982143708 1105 38-39 0.042479617829748406 1105 40-41 -0.1600201710978908 1105 42-43 -0.1131300291192261 1105 44-45 -0.19533253929778027 1105 46-47 -0.2523192706208235 1105 48-49 -0.08374189065420978 1105 50-51 -0.023822219348708984 1105 52-53 0.14537297658598192 1105 54-55 0.09542846771559255 1105 56-57 0.029065749103033056 1105 58-59 0.02228285463352364 1105 60-61 0.07032330485724714 1105 62-63 0.04492200440733285 1105 64-65 0.0389335806244091 1105 66-67 0.25924300880097206 1105 68-69 0.2967731965157405 1105 70-71 0.2642597806417655 1105 72-73 0.09351352794484669 1105 74-75 0.09063870834944865 1105 76-77 0.22816593816009956 1105 78-79 0.282294697868231 1105 80-81 0.31441765958923895 1105 82-83 0.1155063880935927 1105 84-85 0.15778120096009118 1105 86-87 0.26789764817295847 1105 88-89 0.36373793792766307 1105 90-91 0.17600244579988455 1105 92-93 0.22295285594377034 1105 94-95 0.5655332390269443 1105 96-97 0.5579673632998308 1105 98-99 0.8139467044297142 1105 100-101 1.3032733449103588 1106 1 -0.39846413329004804 1106 2 -0.31218991431706655 1106 3 -0.32220833192945264 1106 4 -0.20333116108996308 1106 5 -0.12376769313718228 1106 6 -0.22185926061548855 1106 7 -0.3670926310213929 1106 8 -0.14282703581611145 1106 9 -0.4675135801021497 1106 10-11 -0.4790933502089274 1106 12-13 -0.5812013631079509 1106 14-15 -0.5235304859377194 1106 16-17 -0.40314817096615485 1106 18-19 -0.6090544512441483 1106 20-21 -0.44143924458426653 1106 22-23 -0.5343351326983594 1106 24-25 -0.7012761117983715 1106 26-27 -0.5657852916416175 1106 28-29 -0.6737072244732829 1106 30-31 -0.7163721771745699 1106 32-33 -0.7813118728042951 1106 34-35 -0.7930843256619866 1106 36-37 -0.7626312866593494 1106 38-39 -0.6922092272204665 1106 40-41 -0.8601601942741439 1106 42-43 -0.7906947639718922 1106 44-45 -0.7059627470563115 1106 46-47 -0.41237938401890517 1106 48-49 -0.7117872222953068 1106 50-51 -0.8655856836462519 1106 52-53 -0.6756836120907721 1106 54-55 -0.7192330969571827 1106 56-57 -0.8855744327201691 1106 58-59 -0.9070640717542915 1106 60-61 -1.2273414810383514 1106 62-63 -1.062423349306382 1106 64-65 -1.5049754112087221 1106 66-67 -1.426116981818165 1106 68-69 -1.4228967833429955 1106 70-71 -1.6457701553476376 1106 72-73 -1.7123957260006328 1106 74-75 -1.3032273453421972 1106 76-77 -1.1783478986128841 1106 78-79 -0.9309138676874475 1106 80-81 -1.1029410756496993 1106 82-83 -1.401745605339805 1106 84-85 -1.1782278626090523 1106 86-87 -1.0418113726304874 1106 88-89 -1.3863275901597412 1106 90-91 -1.6564568922974523 1106 92-93 -1.6286398214649047 1106 94-95 -1.6149699132189461 1106 96-97 -2.0444754548217894 1106 98-99 -2.352110091177831 1106 100-101 -1.4619800418571067 1107 1 0.07713024434351468 1107 2 0.062227739435911644 1107 3 -0.017792541684464425 1107 4 0.10760690712166365 1107 5 0.12329547254677209 1107 6 0.16077953906825115 1107 7 0.06625620139134014 1107 8 0.016115721922417947 1107 9 0.1658855489340212 1107 10-11 0.08103101633405174 1107 12-13 0.05985171792208632 1107 14-15 0.027642709942306 1107 16-17 0.03429042825479911 1107 18-19 0.2408974232096952 1107 20-21 0.18057801186157718 1107 22-23 0.1817535449527483 1107 24-25 0.16406689127189367 1107 26-27 0.15543481759975464 1107 28-29 0.09820980057190098 1107 30-31 0.008035344884483209 1107 32-33 0.17177295661235803 1107 34-35 0.0384057027610325 1107 36-37 0.213134063309262 1107 38-39 0.04017428996089478 1107 40-41 -0.07946930320974843 1107 42-43 -0.010648411472168107 1107 44-45 0.08515655183047244 1107 46-47 -0.15033488055331645 1107 48-49 -0.22353817802161302 1107 50-51 -0.3584111778829495 1107 52-53 -0.26841077461941865 1107 54-55 -0.25614909978682476 1107 56-57 -0.12521794183235357 1107 58-59 -0.03443122444072344 1107 60-61 -0.0806078667917447 1107 62-63 -0.09891237360616145 1107 64-65 -0.02927700278928569 1107 66-67 0.014139947084480298 1107 68-69 -0.045914544564297444 1107 70-71 0.06606185393009767 1107 72-73 0.04351955494581361 1107 74-75 0.28964274644009436 1107 76-77 0.08106191212345593 1107 78-79 -0.07011158226677594 1107 80-81 -0.20987115429697667 1107 82-83 -0.1512562083384239 1107 84-85 -0.12496048177858299 1107 86-87 0.07627668626360062 1107 88-89 0.13413933619188967 1107 90-91 0.16879264589631404 1107 92-93 0.12914258593412953 1107 94-95 0.47985188656792843 1107 96-97 0.4014009457492058 1107 98-99 0.47135690211534964 1107 100-101 0.7392439934156627 1108 1 0.14144813312262983 1108 2 0.023676603380089034 1108 3 0.0894745316580483 1108 4 0.10446080030572347 1108 5 0.02496929199333664 1108 6 0.015514902628773086 1108 7 0.07330391406136982 1108 8 0.043557968601227515 1108 9 0.09888549081870934 1108 10-11 0.01840093407626142 1108 12-13 0.1131971405533676 1108 14-15 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-0.31088583613401966 2316 70-71 -0.27899777467393605 2316 72-73 -0.17751655840161717 2316 74-75 -0.21393917211466373 2316 76-77 -0.13350263982155397 2316 78-79 -0.3201110293309064 2316 80-81 -0.3690348498549092 2316 82-83 -0.4573863871357631 2316 84-85 -0.10092933600699183 2316 86-87 0.06739930089826629 2316 88-89 0.07375210414461364 2316 90-91 -0.0779986672600188 2316 92-93 0.08360112901446826 2316 94-95 0.05380816482295003 2316 96-97 0.2562016455448415 2316 98-99 -0.21000938029775185 2316 100-101 0.3266686835504018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 14.0 5 19.0 6 60.0 7 126.0 8 313.0 9 561.0 10 1108.0 11 1966.0 12 3083.0 13 4801.0 14 6428.0 15 9538.0 16 14017.0 17 21507.0 18 18974.0 19 9626.0 20 6862.0 21 6781.0 22 6745.0 23 7069.0 24 7805.0 25 8865.0 26 10181.0 27 12079.0 28 14563.0 29 18056.0 30 22099.0 31 27605.0 32 35213.0 33 45905.0 34 63860.0 35 96347.0 36 144192.0 37 224063.0 38 182119.0 39 15227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44446566142724 19.83828203544276 15.864839757146171 19.85241254598383 2 15.589648639749567 21.403245894911645 40.649178982329396 22.357926483009397 3 19.181225155924164 26.93758738672533 31.243241695576863 22.637945761773643 4 12.143140052587125 17.84135104052835 37.39956193303016 32.61594697385437 5 12.175804541146444 39.37581904899166 33.178105752646964 15.270270657214931 6 29.67498103141389 37.50330459015924 17.06724184827994 15.754472530146929 7 26.933865249073047 35.982381881779176 19.78010756023421 17.303645308913566 8 27.876234151090156 33.96736925991497 18.83630706681237 19.32008952218251 9 27.935788353526725 16.135180404376854 20.5293070620404 35.399724180056026 10-11 24.77039659853882 28.194286041506604 25.696540797969046 21.338776561985522 12-13 26.657453026661653 26.080916502660056 27.579548773434176 19.682081697244115 14-15 23.19531201534666 26.031246868393804 28.02473789947365 22.748703216785888 16-17 19.948605868570365 29.882132307677622 28.46347294530844 21.705788878443574 18-19 19.9203080784703 26.170015795225165 34.15800951531087 19.751666610993666 20-21 21.161899780870865 24.97823978988036 34.63175616348408 19.2281042657647 22-23 20.587591068455417 25.48914622920404 34.64721964134287 19.276043060997672 24-25 20.140983256090948 25.49661953227001 35.077649591900624 19.284747619738418 26-27 20.109613228030828 25.770656868125315 34.612392927870964 19.507336975972894 28-29 19.59339320021875 25.82515802624433 34.992970778864425 19.58847799467249 30-31 19.803203901563776 25.684992627304265 34.980745095606444 19.531058375525514 32-33 19.612849964448813 25.81235654261132 34.70673850074204 19.868054992197827 34-35 20.041659309877506 25.617755550995675 34.73761315537062 19.60297198375621 36-37 20.32412199264257 25.35492746838687 34.47297607475583 19.847974464214733 38-39 20.077115057001198 25.672394622942683 34.49333594201101 19.757154378045115 40-41 19.975242941756967 25.954874200931112 34.369601691955005 19.700281165356916 42-43 20.20942843192144 25.836570601074587 33.9827915295034 19.971209437500566 44-45 20.08460705201926 26.132030903286456 33.65151247631909 20.13184956837519 46-47 20.565240006299 26.594244048158732 32.791841837781604 20.048674107760657 48-49 20.69456042985918 27.08088968633832 31.718959519367047 20.505590364435452 50-51 20.83991467715228 27.20114336433524 31.328994020719897 20.62994793779258 52-53 21.015809541079516 27.633102210854325 30.846738596181467 20.50434965188469 54-55 20.65318743826262 27.676765748698447 30.85174916609801 20.818297646940927 56-57 20.838435366034062 27.874516241404486 30.624555610168112 20.662492782393336 58-59 20.98083099109073 27.31400048674108 30.775827101934077 20.929341420234113 60-61 20.77358427540001 27.214409444685693 31.3784793636099 20.633526916304394 62-63 20.96040695371665 27.33389960726676 31.50842014344546 20.197273295571133 64-65 20.875036386281536 27.320251769208376 31.263283975243013 20.541427869267075 66-67 20.829511779611277 27.614825560587335 30.98378961332716 20.571873046474227 68-69 20.925046646019936 27.548447439121578 31.064722247407627 20.46178366745086 70-71 20.71221672385079 27.756266791374184 30.953248996693024 20.578267488082002 72-73 20.85141512810357 27.735079238584255 30.423655616371683 20.989850016940498 74-75 20.709114942473885 28.06935583158759 30.29915488387408 20.92237434206445 76-77 20.652328483419787 27.935263436678326 30.732306723230433 20.680101356671454 78-79 20.708876343906432 27.872846051432308 30.49413763319765 20.924139971463614 80-81 20.543098059239252 28.157255543837717 30.466126161378526 20.833520235544505 82-83 20.688883427619757 27.962053193291545 30.456941206786713 20.89212217230198 84-85 20.491257237915175 27.871950340767775 30.35028312214098 21.286509299176068 86-87 20.65719589419585 27.605138458748694 30.238169090032784 21.49949655702267 88-89 20.58218050458825 27.632577294005927 30.353316758686184 21.431925442719642 90-91 20.31486833715767 27.530219266261707 30.62931669714683 21.525595699433794 92-93 20.279338513267817 27.53719598267162 30.58937104931373 21.594094454746838 94-95 20.00620356275381 27.41802946215111 30.62655983813473 21.94920713696035 96-97 20.493326398068305 27.305601817166693 30.23750101404391 21.96357077072109 98-99 20.28205388829796 27.47722920445495 30.105456449049107 22.135260458197987 100-101 19.987324029030646 27.35103228506808 30.591327364109155 22.07031632179212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 901.0 1 657.5 2 692.5 3 967.0 4 709.0 5 554.5 6 1267.0 7 3198.5 8 5288.0 9 6046.5 10 4979.5 11 3682.5 12 3391.0 13 3448.0 14 3725.5 15 3888.0 16 3895.5 17 3953.5 18 4107.5 19 4239.5 20 4428.0 21 4853.0 22 5433.5 23 6107.0 24 6664.5 25 7655.0 26 9002.5 27 10290.0 28 11254.0 29 12534.5 30 14928.0 31 17008.5 32 19394.5 33 21639.5 34 23810.5 35 25871.0 36 28633.0 37 31400.0 38 31777.0 39 31588.5 40 32423.5 41 33723.5 42 34161.0 43 35899.5 44 38328.5 45 40184.0 46 39255.0 47 36990.5 48 37818.5 49 36347.0 50 32988.0 51 30105.0 52 27538.0 53 26375.5 54 25098.5 55 23505.0 56 21265.5 57 19024.5 58 17690.0 59 16221.0 60 13842.5 61 11194.0 62 9091.0 63 6951.0 64 4724.0 65 3470.0 66 2656.0 67 1977.5 68 1326.5 69 844.5 70 637.5 71 507.5 72 423.5 73 401.5 74 380.5 75 326.0 76 242.0 77 143.5 78 95.0 79 66.5 80 44.0 81 32.5 82 20.5 83 13.5 84 7.5 85 4.5 86 3.5 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038748407354562246 2 0.0 3 0.0 4 0.0 5 0.0072533964506077105 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006012683899845865 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.54394269816804E-5 22-23 0.0014793111182160462 24-25 9.54394269816804E-5 26-27 9.54394269816804E-4 28-29 0.0015270308317068865 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.77197134908402E-5 38-39 0.0 40-41 2.863182809450412E-4 42-43 0.02094895422247885 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.38598567454201E-4 84-85 3.817577079267216E-4 86-87 0.0 88-89 0.0 90-91 0.010402897541003164 92-93 0.0022905462475603295 94-95 0.0 96-97 0.0 98-99 6.203562753809226E-4 100-101 0.012741163502054332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1047785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.63560848629308 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1425003068958 36.54601225607342 2 13.289054074566906 12.926424622476276 3 4.610137060535198 6.7265046343301975 4 2.123797073455976 4.131686518757595 5 1.1867097930601649 2.885817644106203 6 0.7630926444489496 2.22680850565135 7 0.49900129819498135 1.698846224121417 8 0.36071294399856446 1.4034794816201874 9 0.26642511786269485 1.1661972950956065 >10 1.547834669676615 14.122116008869984 >50 0.1348683953233417 4.529170314107396 >100 0.07090152906958608 6.102014492375975 >500 0.003177659463185889 1.0032058203391756 >1k 0.0011916222986947085 0.9702679053829211 >5k 1.9860371644911807E-4 0.8808235967333855 >10k+ 3.9720743289823615E-4 2.6806246799588678 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15272 1.457550928864223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12480 1.1910840487313714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9119 0.8703121346459435 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.2779196113706533 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.2429887810953583 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.11767681346841194 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1151 0.10985078045591413 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1149 0.10965990160195077 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1054 0.10059315603869114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.54394269816804E-5 0.0 0.0 0.0 0.0 7 9.54394269816804E-5 0.0 0.0 0.0 0.0 8 9.54394269816804E-5 0.0 0.0 0.0 0.0 9 9.54394269816804E-5 0.0 0.0 0.0 0.0 10-11 9.54394269816804E-5 0.0 0.0 0.0 0.0 12-13 9.54394269816804E-5 0.0 0.0 0.0 0.0 14-15 9.54394269816804E-5 0.0 0.0 0.0 0.0 16-17 9.54394269816804E-5 0.0 0.0 0.0 0.0 18-19 9.54394269816804E-5 0.0 0.0 0.0 0.0 20-21 9.54394269816804E-5 0.0 0.0 4.77197134908402E-5 0.0 22-23 9.54394269816804E-5 0.0 0.0 1.908788539633608E-4 0.0 24-25 1.908788539633608E-4 0.0 0.0 7.15795702362603E-4 0.0 26-27 1.908788539633608E-4 0.0 0.0 0.0011452731237801648 0.0 28-29 4.294774214175618E-4 0.0 0.0 0.00243370538803285 0.0 30-31 4.77197134908402E-4 0.0 0.0 0.005249168483992422 0.0 32-33 5.249168483992422E-4 0.0 0.0 0.008112351293442835 0.0 34-35 5.726365618900824E-4 0.0 0.0 0.010736935535439045 0.0 36-37 5.726365618900824E-4 0.0 0.0 0.015842944878958945 0.0 38-39 5.726365618900824E-4 0.0 0.0 0.02357353846447506 0.0 40-41 5.726365618900824E-4 0.0 0.0 0.03268800374122554 0.0 42-43 6.680759888717629E-4 0.0 0.0 0.045715485524224914 0.0 44-45 7.635154158534432E-4 0.0 0.0 0.05511626908192043 0.0 46-47 9.54394269816804E-4 0.0 0.0 0.0727725630735313 0.0 48-49 9.54394269816804E-4 0.0 0.0 0.09792085208320409 0.0 50-51 9.54394269816804E-4 0.0 0.0 0.11257080412489204 0.0 52-53 9.54394269816804E-4 0.0 0.0 0.1406777153709969 0.0 54-55 9.54394269816804E-4 0.0 0.0 0.18080999441679352 0.0 56-57 9.54394269816804E-4 0.0 0.0 0.24208210653903234 0.0 58-59 9.54394269816804E-4 0.0 0.0 0.30454721149854214 0.0 60-61 9.54394269816804E-4 0.0 0.0 0.39659853882237295 0.0 62-63 9.54394269816804E-4 0.0 0.0 0.4842596525050463 0.0 64-65 9.54394269816804E-4 0.0 0.0 0.5665284385632549 0.0 66-67 9.54394269816804E-4 0.0 0.0 0.6853505251554469 0.0 68-69 9.54394269816804E-4 0.0 0.0 0.8439708527989997 0.0 70-71 0.0010021139833076443 0.0 0.0 0.9450412059725993 0.0 72-73 0.0010498336967984844 0.0 0.0 1.0709258101614356 0.0 74-75 0.0010498336967984844 0.0 0.0 1.2210997485171098 0.0 76-77 0.0010498336967984844 0.0 0.0 1.3750435442385602 0.0 78-79 0.0010498336967984844 0.0 0.0 1.548361543637292 0.0 80-81 0.0010498336967984844 0.0 0.0 1.8139217492138178 0.0 82-83 0.0010498336967984844 0.0 0.0 2.1074457068959758 0.0 84-85 0.0012407125507618451 0.0 0.0 2.447830423226139 0.0 86-87 0.0014793111182160464 0.0 0.0 2.881698058284858 0.0 88-89 0.0015270308317068865 0.0 0.0 3.358036238350425 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 50.930866 1 GTATCAA 10230 0.0 39.299805 1 TATCAAC 13450 0.0 29.771015 2 ATCAACG 13550 0.0 29.551306 3 TCAACGC 13690 0.0 29.283796 4 CAACGCA 13930 0.0 28.745169 5 AACGCAG 14220 0.0 28.191002 6 ACGCAGA 16130 0.0 24.766848 7 CGCAGAG 16205 0.0 24.622908 8 GTACATG 8680 0.0 23.815804 1 GCAGAGT 16830 0.0 23.454493 9 TACATGG 8815 0.0 23.332106 2 GAGTACT 10655 0.0 23.227015 12-13 ACATGGG 8885 0.0 22.613682 3 AGTACTT 10880 0.0 22.26642 12-13 GTACTTT 11215 0.0 21.916882 14-15 CGCGGAA 55 0.002879146 21.589619 82-83 CATGGGA 6645 0.0 21.158512 4 ATGGGAT 2390 0.0 21.066692 5 CATATAG 250 9.487849E-9 20.899748 3 >>END_MODULE