FastQCFastQC Report
Thu 26 May 2016
SRR937576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820350
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85431.0413847747912477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.7767416346681295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.5376973243127933No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.18821234838788323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14110.17199975620162128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.15164259157676602No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11480.1399402693972085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.048.8166961
GTATCAA72200.044.3248021
TATCAAC90750.035.087962
ATCAACG91350.034.9615483
TCAACGC91750.034.655854
CAACGCA93150.034.0839655
AACGCAG95700.033.1595546
CGCAGAG107950.029.2188478
ACGCAGA108500.029.2020767
GTAGTAA2400.027.7386781
AGTAACG700.00836793927.157632
GCAGAGT113750.026.9773169
GTACATG72000.025.6912961
GAGTACT61400.025.41157312-13
ATGGGTA3750.025.3486715
TACATGG73350.024.9454042
ACATGGG73350.023.9735053
CATTCCG1201.7095884E-423.7513149
AGTACTT63550.023.5802512-13
GTACTTT66400.023.49876814-15
Produced by FastQC (version 0.11.5)