##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937576_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 820350 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926001097092705 33.0 31.0 34.0 30.0 34.0 2 32.008146522825626 33.0 31.0 34.0 30.0 34.0 3 32.02899616017554 34.0 31.0 34.0 30.0 34.0 4 35.65151094045225 37.0 35.0 37.0 35.0 37.0 5 35.67235448284269 37.0 35.0 37.0 35.0 37.0 6 35.762765892606815 37.0 35.0 37.0 35.0 37.0 7 35.695153288230635 37.0 35.0 37.0 35.0 37.0 8 35.70349484975925 37.0 35.0 37.0 35.0 37.0 9 37.253894069604435 39.0 37.0 39.0 34.0 39.0 10-11 37.208190406533795 39.0 37.0 39.0 34.0 39.0 12-13 37.116702017431585 39.0 37.0 39.0 34.0 39.0 14-15 38.214059852501975 40.0 38.0 41.0 33.5 41.0 16-17 38.15516182117389 40.0 38.0 41.0 33.0 41.0 18-19 38.17286645943804 40.0 38.0 41.0 33.5 41.0 20-21 38.232344121411586 40.0 38.0 41.0 34.0 41.0 22-23 38.21737307246907 40.0 38.0 41.0 34.0 41.0 24-25 38.14596269884805 40.0 38.0 41.0 34.0 41.0 26-27 38.03317608337905 40.0 38.0 41.0 33.5 41.0 28-29 37.878845005180715 40.0 38.0 41.0 33.0 41.0 30-31 37.580410800268176 40.0 37.0 41.0 33.0 41.0 32-33 37.354563905650025 40.0 36.0 41.0 33.0 41.0 34-35 37.29482842689096 40.0 36.0 41.0 33.0 41.0 36-37 37.113975742061314 40.0 36.0 41.0 32.0 41.0 38-39 36.925000914243924 40.0 36.0 41.0 31.5 41.0 40-41 36.653301030048155 39.0 35.0 41.0 31.0 41.0 42-43 36.313265679283234 39.0 35.0 41.0 30.0 41.0 44-45 35.91859572133845 39.0 35.0 41.0 29.5 41.0 46-47 35.35221368927897 39.0 35.0 40.5 26.5 41.0 48-49 34.845898092277686 39.0 35.0 40.0 24.0 41.0 50-51 33.92466873895289 38.0 34.0 39.5 22.5 40.5 52-53 33.9949277747303 38.0 34.0 39.5 22.0 40.5 54-55 34.238840129213145 38.0 34.0 40.0 21.5 41.0 56-57 34.02116596574633 38.0 34.0 40.0 19.5 41.0 58-59 33.74569208264765 38.0 34.0 40.0 18.0 41.0 60-61 33.41085085634181 38.0 33.5 40.0 15.0 41.0 62-63 33.06004083622844 37.0 33.0 40.0 11.0 41.0 64-65 32.623979399037 36.5 32.5 39.5 7.5 41.0 66-67 32.13281830925824 36.0 32.0 39.0 6.0 40.5 68-69 31.684738830986774 35.5 31.0 39.0 2.0 40.0 70-71 31.225852989577618 35.0 31.0 38.0 2.0 40.0 72-73 30.77340403486317 35.0 30.5 37.5 2.0 40.0 74-75 30.241893703906868 35.0 30.0 37.0 2.0 39.0 76-77 29.72036935454379 34.0 29.0 36.5 2.0 39.0 78-79 29.225372706771502 34.0 29.0 36.0 2.0 38.5 80-81 28.712500152373988 34.0 28.5 35.5 2.0 37.0 82-83 28.20795575059426 34.0 27.5 35.0 2.0 37.0 84-85 27.693752666544768 34.0 26.5 35.0 2.0 36.0 86-87 27.126717864326203 33.0 25.5 35.0 2.0 36.0 88-89 26.615547022612297 33.0 25.0 35.0 2.0 35.5 90-91 26.071774242701288 32.5 23.5 35.0 2.0 35.0 92-93 25.430027427317608 32.0 20.5 34.5 2.0 35.0 94-95 24.702285609800697 31.5 17.0 34.0 2.0 35.0 96-97 23.859993295544584 31.0 4.5 34.0 2.0 35.0 98-99 22.736337538855366 31.0 2.0 34.0 2.0 35.0 100-101 19.61489242396538 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14192289935941815 1101 2 0.02616109071974293 1101 3 0.019508282239641517 1101 4 0.03447299946036253 1101 5 0.09065542873032939 1101 6 0.022719842985686967 1101 7 -0.07323293883733584 1101 8 0.03716981260936336 1101 9 0.18993827473755687 1101 10-11 0.19242120884443636 1101 12-13 0.143234443685202 1101 14-15 0.27803688021083417 1101 16-17 0.233658167670022 1101 18-19 0.24477146237109082 1101 20-21 0.24614312723479514 1101 22-23 0.06119006816421546 1101 24-25 0.12748511067947987 1101 26-27 0.0757149632455949 1101 28-29 0.1240826884890609 1101 30-31 0.1704162373596958 1101 32-33 0.28701922613257835 1101 34-35 0.27380475095813495 1101 36-37 0.1716704162364664 1101 38-39 0.29670879547450824 1101 40-41 0.15556591716869406 1101 42-43 0.14149504696876392 1101 44-45 0.11538513855860089 1101 46-47 0.3974486445047489 1101 48-49 0.22263319328569509 1101 50-51 0.23002758053484484 1101 52-53 0.3347367267492132 1101 54-55 0.46950811387015534 1101 56-57 0.5662279960700971 1101 58-59 0.530038701874112 1101 60-61 0.5211055763158967 1101 62-63 0.5790888648527073 1101 64-65 0.5118335392492313 1101 66-67 0.8514446003012672 1101 68-69 0.9221692299658457 1101 70-71 0.8318944754212687 1101 72-73 0.7096736337250391 1101 74-75 0.8280462287770085 1101 76-77 0.8432228552580803 1101 78-79 1.0310627522512412 1101 80-81 1.2273138904665295 1101 82-83 1.2575895392529652 1101 84-85 1.6212256448550697 1101 86-87 1.8495096187484243 1101 88-89 1.8051345017760774 1101 90-91 1.819985960303729 1101 92-93 1.8471883970747989 1101 94-95 2.2036999970920412 1101 96-97 2.344414856171479 1101 98-99 2.7870262385260816 1101 100-101 3.373340913350674 1102 1 0.310247557275094 1102 2 0.28969953516993385 1102 3 0.19561114566001692 1102 4 0.33570348357453383 1102 5 0.17974494352063175 1102 6 0.17146356846006938 1102 7 0.1942159101799632 1102 8 0.17598689240443832 1102 9 0.36312278953547406 1102 10-11 0.34770915612548947 1102 12-13 0.32942942223883875 1102 14-15 0.3644906758138333 1102 16-17 0.36691775614939104 1102 18-19 0.43893370003544163 1102 20-21 0.3796461650257328 1102 22-23 0.2268790068925881 1102 24-25 0.3325751793169758 1102 26-27 0.3671895965881049 1102 28-29 0.4190352234810959 1102 30-31 0.4277444420275387 1102 32-33 0.539444970288649 1102 34-35 0.5916886895419609 1102 36-37 0.644735032248434 1102 38-39 0.6160747317425219 1102 40-41 0.5570320906476312 1102 42-43 0.7909679792092064 1102 44-45 0.8288943690914081 1102 46-47 0.9728890214860044 1102 48-49 1.1134009106897267 1102 50-51 1.129641256908549 1102 52-53 1.1116024994497238 1102 54-55 1.082573962344263 1102 56-57 1.287176505019147 1102 58-59 1.1989894809879829 1102 60-61 1.2465852855765505 1102 62-63 1.2447344391724542 1102 64-65 1.203454774724058 1102 66-67 1.2859194573128327 1102 68-69 1.2904095174794747 1102 70-71 1.4625613287324022 1102 72-73 1.4537581958434167 1102 74-75 1.4081475189062083 1102 76-77 1.422421100597166 1102 78-79 1.5992479593581344 1102 80-81 1.7878829380167431 1102 82-83 1.8503836678641932 1102 84-85 2.0139961132363347 1102 86-87 1.8327199848201126 1102 88-89 1.7093299702714546 1102 90-91 1.9720706471898772 1102 92-93 2.1109288283756875 1102 94-95 2.073768436779755 1102 96-97 2.306083762064624 1102 98-99 2.5461742267979126 1102 100-101 2.6966511088556864 1103 1 0.13817513834964146 1103 2 0.09038002766620323 1103 3 0.21735388256077215 1103 4 0.09555599136466952 1103 5 0.19428835778374065 1103 6 0.19288077077199262 1103 7 0.2272983627700782 1103 8 0.15711136399763603 1103 9 0.08040147687984955 1103 10-11 0.06540728226701731 1103 12-13 0.05975805566654202 1103 14-15 0.2012190375453642 1103 16-17 0.23796145447273176 1103 18-19 0.1513810158619009 1103 20-21 0.1001035098216434 1103 22-23 0.08655364446146763 1103 24-25 0.06793903464814832 1103 26-27 0.12046215824796036 1103 28-29 0.20443671874911473 1103 30-31 0.1808225437226625 1103 32-33 0.058009832272944095 1103 34-35 0.07012593570714643 1103 36-37 0.35324854428147745 1103 38-39 0.11647930237334947 1103 40-41 0.2002665271988988 1103 42-43 0.11278278758985039 1103 44-45 0.18027256985546813 1103 46-47 0.2864758648803942 1103 48-49 0.17042541499289143 1103 50-51 0.2253092992718635 1103 52-53 0.2620808631375908 1103 54-55 0.29563048172217776 1103 56-57 0.3037756389093289 1103 58-59 0.28747463395011863 1103 60-61 0.5511354139905578 1103 62-63 0.44730968383204583 1103 64-65 0.3971087283259962 1103 66-67 0.6016629673582337 1103 68-69 0.8207863550674546 1103 70-71 0.7831495133919013 1103 72-73 0.6397526961869495 1103 74-75 0.8055779084991137 1103 76-77 0.9444263780136772 1103 78-79 0.9017568450438489 1103 80-81 1.045647250974433 1103 82-83 0.8025273444330558 1103 84-85 1.137500493085117 1103 86-87 1.166552575527664 1103 88-89 1.5336042863749313 1103 90-91 1.4457215176036335 1103 92-93 1.480058054651959 1103 94-95 1.3940267829075381 1103 96-97 1.4277460543602523 1103 98-99 2.163185913176637 1103 100-101 3.186671047849252 1104 1 -0.01623657174965132 1104 2 -0.07660474058997835 1104 3 -9.653032971996822E-4 1104 4 0.0579011798615241 1104 5 -0.08577471792443703 1104 6 0.0927765522745716 1104 7 0.1317540129722019 1104 8 0.10672753791627798 1104 9 0.26060788396571866 1104 10-11 0.1787801740703827 1104 12-13 0.08841319146002746 1104 14-15 0.28446510954771753 1104 16-17 0.3763591631510863 1104 18-19 0.26224994067852947 1104 20-21 0.21144649264910953 1104 22-23 0.11866828975963273 1104 24-25 0.010882501683170176 1104 26-27 0.175925707766595 1104 28-29 0.0015151351230500154 1104 30-31 0.2337771752570461 1104 32-33 0.1428080419922395 1104 34-35 0.129924083538981 1104 36-37 0.3403177765550467 1104 38-39 0.4178415693360904 1104 40-41 0.4117223927694198 1104 42-43 0.46897735188969136 1104 44-45 0.6670187495420237 1104 46-47 0.7778116192515085 1104 48-49 0.7388902839523652 1104 50-51 0.894991123417384 1104 52-53 0.7358640936139551 1104 54-55 0.6947303903762716 1104 56-57 0.8077871846355009 1104 58-59 0.9140072771144006 1104 60-61 1.1398449117366098 1104 62-63 1.1711554206518713 1104 64-65 1.3469662633728703 1104 66-67 1.3576084964837172 1104 68-69 1.141628321702683 1104 70-71 1.1115256604761363 1104 72-73 1.2551369307256017 1104 74-75 0.9838732305491256 1104 76-77 1.1004013655742924 1104 78-79 1.2252240781046275 1104 80-81 1.361565450593698 1104 82-83 1.4655018047124422 1104 84-85 1.474306951166259 1104 86-87 1.5868835992206591 1104 88-89 1.559541324539694 1104 90-91 1.5646036318776098 1104 92-93 1.7825045900676848 1104 94-95 1.7715193007125656 1104 96-97 1.9595128920838825 1104 98-99 2.103010251785257 1104 100-101 2.413504711661666 1105 1 0.18421898072456955 1105 2 0.062001202365213004 1105 3 0.14007835433423566 1105 4 0.11863320054730053 1105 5 -0.024962721086090767 1105 6 0.10354944389026599 1105 7 0.20795259064306748 1105 8 0.11598815319255351 1105 9 0.3743526686377123 1105 10-11 0.2944428284269591 1105 12-13 0.18895152444541452 1105 14-15 0.2922627844228529 1105 16-17 0.3086240358591752 1105 18-19 0.13973686146162834 1105 20-21 0.11205275343160537 1105 22-23 0.2091457266174146 1105 24-25 0.17295053957732165 1105 26-27 0.21424467135875602 1105 28-29 0.20363252972850177 1105 30-31 -0.1103451487876228 1105 32-33 0.23293753065380685 1105 34-35 0.1474637885827761 1105 36-37 0.16846148850795117 1105 38-39 0.27240753252421257 1105 40-41 0.49243442980357344 1105 42-43 0.48637683900366113 1105 44-45 0.5303727416503534 1105 46-47 0.4267028070220178 1105 48-49 0.4288279337129026 1105 50-51 0.3968830268186565 1105 52-53 0.38633610871178803 1105 54-55 0.40486046068257764 1105 56-57 0.4039579887697542 1105 58-59 0.4022107946110012 1105 60-61 0.49934966200847697 1105 62-63 0.7509196883455544 1105 64-65 0.5135633404002888 1105 66-67 0.7409103067491252 1105 68-69 0.525402198998492 1105 70-71 0.49531719484458847 1105 72-73 0.4097832855268173 1105 74-75 0.3389356690739511 1105 76-77 0.3542129562999712 1105 78-79 0.5520959615014753 1105 80-81 0.6290763330630291 1105 82-83 0.612966924204084 1105 84-85 0.9658694670706076 1105 86-87 0.6911512098136576 1105 88-89 0.6840122615174486 1105 90-91 0.8108429350672033 1105 92-93 1.100368250461937 1105 94-95 1.1801351212419213 1105 96-97 1.2774461421173804 1105 98-99 1.6460581948920883 1105 100-101 2.2615053597800987 1106 1 -0.18276496861678737 1106 2 -0.1420836931142233 1106 3 -0.024380942314596865 1106 4 -0.1073921630335235 1106 5 -0.07957835606065089 1106 6 -0.09542504978026045 1106 7 -0.16349653659165853 1106 8 -0.12102742925050336 1106 9 -0.18274251045474443 1106 10-11 -0.554829581684217 1106 12-13 -0.5368440138026571 1106 14-15 -0.7897049201217001 1106 16-17 -0.522231656671643 1106 18-19 -0.10496359476307049 1106 20-21 -0.22246230084143548 1106 22-23 -0.4027570843890729 1106 24-25 -0.513058093861261 1106 26-27 -0.38132912113038486 1106 28-29 -0.20475352544099934 1106 30-31 -0.2136252273564807 1106 32-33 -0.1507158927444081 1106 34-35 -0.0746138609749849 1106 36-37 -0.06795954579169461 1106 38-39 -0.21148756434502758 1106 40-41 0.16398333834736434 1106 42-43 0.08691328844494706 1106 44-45 -0.39238453424853503 1106 46-47 -0.12750813551880213 1106 48-49 0.03019018411688279 1106 50-51 -0.08705489464674798 1106 52-53 -0.15178349182038176 1106 54-55 -0.22543051817348925 1106 56-57 -0.5707826369261468 1106 58-59 -0.7468409260812763 1106 60-61 -0.33856566519998665 1106 62-63 -0.4787636094909473 1106 64-65 -0.45576140136739696 1106 66-67 -0.3672082374430019 1106 68-69 -0.42060597764440644 1106 70-71 -0.3014358442742484 1106 72-73 -0.37763898783114414 1106 74-75 -0.3842818284850473 1106 76-77 0.04363399630956266 1106 78-79 0.20596473551893624 1106 80-81 0.18355421558659657 1106 82-83 0.04332485217882365 1106 84-85 -0.22509040342781717 1106 86-87 0.1608356781891196 1106 88-89 0.5426718231077814 1106 90-91 0.5783895996021577 1106 92-93 0.5397350459830541 1106 94-95 0.40116071322058744 1106 96-97 0.6487011311828361 1106 98-99 0.8574946460463586 1106 100-101 1.6098677472227862 1107 1 0.03004400008458674 1107 2 0.18377147103138114 1107 3 0.24478213193992815 1107 4 0.293106523291506 1107 5 0.24500777255195771 1107 6 0.24482952257389456 1107 7 0.15421511812863997 1107 8 0.16849760709875028 1107 9 0.20748910447268543 1107 10-11 0.42194196641159465 1107 12-13 0.33758205052915713 1107 14-15 0.2897947355168995 1107 16-17 0.14952496909679525 1107 18-19 0.39996587492037605 1107 20-21 0.3255633604600021 1107 22-23 0.26463125323815007 1107 24-25 0.30874763529212146 1107 26-27 0.2121235483081776 1107 28-29 0.218960858662264 1107 30-31 0.0406601589659914 1107 32-33 0.1681500103752498 1107 34-35 0.16127369865055385 1107 36-37 0.2654205526701574 1107 38-39 0.22630737481779306 1107 40-41 0.23644271645618886 1107 42-43 0.4469558872256485 1107 44-45 0.49726357941623434 1107 46-47 0.5347020269687661 1107 48-49 0.5988392670952223 1107 50-51 0.5481292749321156 1107 52-53 0.49940072877498665 1107 54-55 0.44096618616035954 1107 56-57 0.5324109962141748 1107 58-59 0.23848864053352514 1107 60-61 0.559174941386253 1107 62-63 0.6898331815709682 1107 64-65 0.5465439068998208 1107 66-67 0.7771078863230336 1107 68-69 0.8000021390813572 1107 70-71 0.9136433588479598 1107 72-73 1.0498219207727892 1107 74-75 1.1761567859067092 1107 76-77 1.0183011136220124 1107 78-79 1.0153543230505022 1107 80-81 1.3436312505073928 1107 82-83 1.5257592322007625 1107 84-85 1.4304908020921694 1107 86-87 1.445938221361839 1107 88-89 1.2790854904291606 1107 90-91 1.4004791080581391 1107 92-93 1.3529126089246652 1107 94-95 1.5851134719380333 1107 96-97 1.7771606878494133 1107 98-99 1.588280555287998 1107 100-101 2.1853434761210835 1108 1 -0.025164474721876218 1108 2 0.06969432856227442 1108 3 0.1627503514156814 1108 4 0.21182172090117746 1108 5 0.2187684679206896 1108 6 0.2126076021610217 1108 7 0.26435364861892907 1108 8 0.18063260641968526 1108 9 0.2513842559055206 1108 10-11 0.2275205349298659 1108 12-13 0.3459142520774279 1108 14-15 0.1546324375405348 1108 16-17 0.2631607153958484 1108 18-19 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-0.998824913406132 2316 50-51 -0.7424262966219288 2316 52-53 -0.6990323391434075 2316 54-55 -0.9587148839466195 2316 56-57 -1.0472854804384326 2316 58-59 -1.0469860106594027 2316 60-61 -1.1235114130218946 2316 62-63 -1.2553169962522475 2316 64-65 -1.1029949766295672 2316 66-67 -0.9022739800287845 2316 68-69 -1.137003807557278 2316 70-71 -1.0876597181415733 2316 72-73 -1.3087456304503746 2316 74-75 -1.3754749400583641 2316 76-77 -1.3339004385514635 2316 78-79 -1.3700864913366182 2316 80-81 -1.405614620682364 2316 82-83 -1.5312809832029046 2316 84-85 -1.5878836633582942 2316 86-87 -1.6747205884465153 2316 88-89 -1.6954505133720836 2316 90-91 -1.7589120454169738 2316 92-93 -1.9892836877449263 2316 94-95 -1.7915999663133952 2316 96-97 -1.9635557885152153 2316 98-99 -2.508820278393305 2316 100-101 -2.3006052503191405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3086.0 3 557.0 4 448.0 5 480.0 6 604.0 7 762.0 8 907.0 9 1137.0 10 1522.0 11 2072.0 12 3024.0 13 4426.0 14 5142.0 15 7259.0 16 9957.0 17 14481.0 18 11694.0 19 6474.0 20 6016.0 21 6064.0 22 6452.0 23 7059.0 24 7928.0 25 8752.0 26 10049.0 27 11692.0 28 13828.0 29 16880.0 30 20389.0 31 25515.0 32 32084.0 33 42881.0 34 58646.0 35 83974.0 36 117909.0 37 154673.0 38 106920.0 39 8607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98503808653819 19.866877490849816 15.02195677615094 20.12612764646105 2 15.007871667375605 21.034464213248846 41.38235768660986 22.575306432765693 3 19.16936812716667 27.53521677692537 30.200494819552215 23.094920276355744 4 11.943314147139244 17.195458384619428 37.81717419018901 33.044053278052324 5 12.261269310829954 39.63372524381852 33.15846278520869 14.946542660142839 6 29.590158927938827 37.245941290724524 17.78307130379546 15.380828477541197 7 27.385489190179552 35.66581165262001 19.99120556980579 16.95749358739465 8 27.351750169489453 34.34338868970909 19.236289677328724 19.068571463472733 9 28.078722055343885 16.685987635394387 20.506897524757917 34.72839278450381 10-11 24.504273948004116 28.47631234176925 25.971311563022027 21.048102147204602 12-13 26.23257620780833 26.29567169822154 27.45863214107492 20.013119952895217 14-15 23.075029668488614 26.531599459828897 27.363660850238112 23.029710021444377 16-17 20.18835970951491 30.68396890434158 27.560051032155265 21.567620353988247 18-19 20.243923738869324 27.66020205449963 32.23082555562545 19.8650486510056 20-21 21.630704347661467 26.468014370678244 32.3633390147548 19.537942266905485 22-23 21.15027217444571 26.927039659355955 32.256504184456894 19.66618398174144 24-25 20.395012870897116 27.09929981098255 32.78246504398702 19.723222274133313 26-27 20.224099421673 27.43709342585914 32.364446784327214 19.97436036814065 28-29 19.910188510066227 27.497072758667656 32.60852170800638 19.984217023259735 30-31 20.125067035100127 27.282827653521068 32.69144552718601 19.900659784192786 32-33 19.807612787293575 27.523684980445502 32.34741351034895 20.321288721911973 34-35 20.236497732443617 27.484860052833298 32.29482813907679 19.983814075646293 36-37 20.53639425557968 27.15310413059609 32.120751306174675 20.189750307649557 38-39 20.226813894381497 27.408256460550305 32.1977779053393 20.16715173972889 40-41 20.136782616394985 27.59229010127409 32.168645638374855 20.102281643956072 42-43 20.26757327516187 27.625991017784884 31.73974538583585 20.366690321217398 44-45 20.13252393980848 28.00078170803205 31.40792945085011 20.45876490130936 46-47 20.60751159658836 28.235883310379368 30.73195161649876 20.424653476533514 48-49 20.68771538060295 28.563636607953246 30.023979702153287 20.724668309290518 50-51 20.835127464219685 28.874177524594096 29.62383412030785 20.666860890878365 52-53 20.868140137753095 29.02710349562528 29.46439260853737 20.64036375808426 54-55 20.62295166071989 29.15992284868644 29.347548589081306 20.869576901512367 56-57 20.81680629167379 29.412109518110547 29.109066262853233 20.66201792736243 58-59 20.86115983313563 28.78666794466589 29.317261475588484 21.034910746609995 60-61 20.585383427060627 28.91545004365799 29.7957538788445 20.703412650436885 62-63 20.789042112936965 28.942920955359337 29.902847297100926 20.365189634602775 64-65 20.63064350281244 29.0086730120582 29.78444475381688 20.57623873131248 66-67 20.59497182313391 29.14737884008644 29.505749253064224 20.751900083715427 68-69 20.482034379737776 29.18565339269872 29.578810661101368 20.75350156646214 70-71 20.414797699720463 29.29809478333824 29.473461178203276 20.81364633873802 72-73 20.537445956178704 29.43403600478761 29.01573072131708 21.0127873177166 74-75 20.312922718958877 29.847783006048985 29.01613164250839 20.823162632483747 76-77 20.463354889220977 29.649961617125175 29.11803665335531 20.768646840298537 78-79 20.502722402847226 29.591871630583732 28.792860440518737 21.112545526050305 80-81 20.260193422983466 29.84139119941913 28.841055942685145 21.057359434912257 82-83 20.43098074007497 29.62870469478609 29.080148227479967 20.860166337658974 84-85 20.35203171069607 29.5486586636887 28.843874766684962 21.255434858930265 86-87 20.617210827445977 29.292295855748034 28.84294341419927 21.24754990260672 88-89 20.59384621773099 29.240053201039107 28.79945919604876 21.36664138518114 90-91 20.215301436055327 29.283022647014462 29.17786946961153 21.323806447318685 92-93 20.440547087332103 29.437054975572313 28.889943218836766 21.23245471825882 94-95 20.126826527932977 29.288806658240063 28.891290675166548 21.693076138660413 96-97 20.42982845578451 29.311215563373526 28.593613457283713 21.665342523558245 98-99 20.36236260565449 29.593577159760642 28.2330532979223 21.811006936662572 100-101 20.56524460047317 28.934259329924444 28.996229871021907 21.504266198580478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1842.0 1 1114.0 2 463.5 3 588.5 4 562.0 5 608.0 6 1033.0 7 1890.5 8 2934.5 9 3432.0 10 3074.5 11 2616.0 12 2642.5 13 2732.0 14 2751.0 15 2823.5 16 2809.5 17 2859.0 18 3027.0 19 3149.0 20 3326.5 21 3628.0 22 4148.0 23 4734.0 24 5272.5 25 6100.0 26 7223.5 27 8269.0 28 9138.0 29 10352.5 30 12080.0 31 13783.5 32 15953.5 33 17934.0 34 19809.0 35 21305.0 36 23263.5 37 25411.0 38 25442.5 39 25024.5 40 25585.5 41 26708.5 42 27209.0 43 27997.0 44 29729.0 45 31257.0 46 30280.5 47 28690.0 48 29361.5 49 28204.0 50 25646.0 51 23284.0 52 21048.0 53 20224.5 54 19336.0 55 18008.5 56 16461.5 57 14807.5 58 13638.5 59 12305.5 60 10550.5 61 8858.0 62 7200.5 63 5392.0 64 3732.5 65 2753.5 66 2055.0 67 1533.5 68 1113.0 69 722.0 70 519.0 71 412.0 72 311.5 73 271.0 74 235.0 75 197.0 76 152.0 77 108.0 78 74.0 79 47.0 80 30.0 81 17.0 82 11.5 83 8.0 84 7.0 85 5.0 86 2.0 87 2.0 88 4.5 89 3.5 90 2.5 91 2.0 92 2.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3177911866886085 2 0.27134759553848964 3 0.27719875662826843 4 0.2714694947278601 5 0.27073809959163775 6 0.2121045895044798 7 0.2011336624611446 8 0.20905710977022005 9 0.21003230328518313 10-11 0.2105808496373499 12-13 0.2132626318034985 14-15 0.20972755531175719 16-17 0.20119461205582984 18-19 0.1963186444810142 20-21 0.1949777533979399 22-23 0.2030230998963857 24-25 0.2002194185408667 26-27 0.2066800755774974 28-29 0.21289693423538733 30-31 0.21320168220881333 32-33 0.21216553909916497 34-35 0.2117998415310538 36-37 0.2002194185408667 38-39 0.19174742487962454 40-41 0.18681050771012372 42-43 0.1975985859694033 44-45 0.19869567867373683 46-47 0.20509538611568232 48-49 0.2122264886938502 50-51 0.20826476503931246 52-53 0.217468153836777 54-55 0.2071067227402938 56-57 0.18162979216188213 58-59 0.1659657463277869 60-61 0.18102029621503016 62-63 0.17468153836776987 64-65 0.18138599378314132 66-67 0.1833973304077528 68-69 0.17876516121167796 70-71 0.1819954897299933 72-73 0.19138172731151337 74-75 0.19717193880660697 76-77 0.19814713232157005 78-79 0.19357591272018043 80-81 0.19193027366368015 82-83 0.17961845553727068 84-85 0.17632717742427012 86-87 0.183458280002438 88-89 0.19125982812214298 90-91 0.18827329798256842 92-93 0.1833973304077528 94-95 0.1846772718961419 96-97 0.19278356798927287 98-99 0.19314926555738404 100-101 0.17218260498567686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 820350.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91138980781513 #Duplication Level Percentage of deduplicated Percentage of total 1 78.49644355495951 43.103488105735394 2 12.010660983910002 13.190441742739972 3 3.867635548587698 6.371317297291861 4 1.7100127723754965 3.755967116810141 5 0.9638693230746682 2.6463702061574006 6 0.5974601955164409 1.9684421814394035 7 0.39482889007004296 1.517642216301618 8 0.2870023874199146 1.2607759977110804 9 0.23257873111794106 1.1494099228881856 >10 1.2808256741577189 13.042968293987009 >50 0.10771743093499295 4.0680765967644765 >100 0.047626221337003755 4.327581383180769 >500 0.0017804194872430284 0.5823146186663559 >1k 0.0011127621795268927 1.19249235025106 >5k 4.451048718107571E-4 1.8227119700752779 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8543 1.0413847747912477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6372 0.7767416346681295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4411 0.5376973243127933 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1544 0.18821234838788323 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1411 0.17199975620162128 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1244 0.15164259157676602 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1148 0.1399402693972085 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2189918937039069E-4 0.0 24-25 0.0 0.0 0.0 4.8759675748156274E-4 0.0 26-27 0.0 0.0 0.0 0.0012189918937039069 0.0 28-29 6.094959468519534E-5 0.0 0.0 0.0027427317608337905 0.0 30-31 1.2189918937039069E-4 0.0 0.0 0.005912110684463948 0.0 32-33 1.2189918937039069E-4 0.0 0.0 0.008959590418723715 0.0 34-35 1.2189918937039069E-4 0.0 0.0 0.011702322179557505 0.0 36-37 1.2189918937039069E-4 0.0 0.0 0.015237398671298835 0.0 38-39 1.2189918937039069E-4 0.0 0.0 0.022612299628207475 0.0 40-41 1.2189918937039069E-4 0.0 0.0 0.030413847747912476 0.0 42-43 3.047479734259767E-4 0.0 0.0 0.04248186749558115 0.0 44-45 4.8759675748156274E-4 0.0 0.0 0.05199000426647163 0.0 46-47 4.8759675748156274E-4 0.0 0.0 0.070823429024197 0.0 48-49 4.8759675748156274E-4 0.0 0.0 0.09745840190162736 0.0 50-51 4.8759675748156274E-4 0.0 0.0 0.11172060705796306 0.0 52-53 4.8759675748156274E-4 0.0 0.0 0.13738038642043032 0.0 54-55 4.8759675748156274E-4 0.0 0.0 0.18382397757054914 0.0 56-57 6.094959468519534E-4 0.0 0.0 0.2526970195648199 0.0 58-59 6.094959468519534E-4 0.0 0.0 0.3230938014262205 0.0 60-61 6.094959468519534E-4 0.0 0.0 0.4244529773877004 0.0 62-63 6.094959468519534E-4 0.0 0.0 0.5195952946912903 0.0 64-65 6.094959468519534E-4 0.0 0.0 0.6107149387456574 0.0 66-67 7.313951362223441E-4 0.0 0.0 0.7458401901627354 0.0 68-69 7.313951362223441E-4 0.0 0.0 0.9333211434143963 0.0 70-71 7.313951362223441E-4 0.0 0.0 1.0465045407448041 0.0 72-73 7.923447309075395E-4 0.0 0.0 1.2004022673249222 0.0 74-75 8.532943255927348E-4 0.0 0.0 1.374657158529896 0.0 76-77 8.532943255927348E-4 0.0 0.0 1.5451941244590723 0.0 78-79 8.532943255927348E-4 0.0 1.2189918937039069E-4 1.7325531785213628 0.0 80-81 0.0010970927043335162 0.0 1.2189918937039069E-4 2.041811421954044 0.0 82-83 0.0010970927043335162 0.0 1.2189918937039069E-4 2.3914182970683244 0.0 84-85 0.0011580422990187115 0.0 1.2189918937039069E-4 2.7902724446882425 0.0 86-87 0.0012799414883891023 0.0 1.2189918937039069E-4 3.2940208447613823 0.0 88-89 0.0013408910830742975 0.0 1.2189918937039069E-4 3.8763942219784235 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3760 0.0 48.816696 1 GTATCAA 7220 0.0 44.324802 1 TATCAAC 9075 0.0 35.08796 2 ATCAACG 9135 0.0 34.961548 3 TCAACGC 9175 0.0 34.65585 4 CAACGCA 9315 0.0 34.083965 5 AACGCAG 9570 0.0 33.159554 6 CGCAGAG 10795 0.0 29.218847 8 ACGCAGA 10850 0.0 29.202076 7 GTAGTAA 240 0.0 27.738678 1 AGTAACG 70 0.008367939 27.15763 2 GCAGAGT 11375 0.0 26.977316 9 GTACATG 7200 0.0 25.691296 1 GAGTACT 6140 0.0 25.411573 12-13 ATGGGTA 375 0.0 25.348671 5 TACATGG 7335 0.0 24.945404 2 ACATGGG 7335 0.0 23.973505 3 CATTCCG 120 1.7095884E-4 23.751314 9 AGTACTT 6355 0.0 23.58025 12-13 GTACTTT 6640 0.0 23.498768 14-15 >>END_MODULE