FastQCFastQC Report
Thu 26 May 2016
SRR937576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820350
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127011.5482416041933322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102621.2509294813189493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75060.9149753154141524No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.2917047601633449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.26915341012982263No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.13043213262631803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.12640945937709513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9990.12177729018102029No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.11958310477235326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44000.052.696871
GTATCAA81050.041.6806221
CTCGTAC1255.646143E-934.2014278
CCTCGTA1308.278221E-932.8859867
TCGTACC1201.504377E-731.6679869
TATCAAC106950.031.5347422
ATCAACG107300.031.387613
TCAACGC108250.031.1560334
CAACGCA109950.030.6743135
AACGCAG111500.030.3312556
TAGGGCG650.005841918829.2319894
GTACATG65650.028.225881
TACATGG67100.027.2552362
CGTACAC700.008388489527.143993
GTGTAGC3350.026.9479921
CGCAGAG126700.026.5816148
ACGCAGA127000.026.5562257
GGGGCTA1806.690243E-926.389995
ACATGGG66450.026.3780753
ATGGGAG17900.026.0066725
Produced by FastQC (version 0.11.5)