FastQCFastQC Report
Thu 26 May 2016
SRR937575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227404
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70100.3147161448933377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.24750786116932538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.15228490206536396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31310.140567225343943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43050.045.1896551
GTATCAA67600.038.8401831
TATCAAC84650.030.7250582
ATCAACG84950.030.5612683
TCAACGC85750.030.4438744
CAACGCA87100.029.7536335
AACGCAG88050.029.563336
GTACATG64150.025.8772241
ACGCAGA102800.025.044837
TACATGG65950.024.8013882
CGCAGAG103800.024.6651528
ACATGGG65700.023.0146243
GAGTACT65850.022.325812-13
GTATAGG4550.021.9531481
GCAGAGT115800.021.698999
GTGGTAT21600.021.1400661
TATTCCG2709.895302E-1021.1338775
AGTACTT69050.021.01598212-13
AGAGTAC99250.020.41224110-11
GTACTTT73350.020.04344714-15
Produced by FastQC (version 0.11.5)