FastQCFastQC Report
Thu 26 May 2016
SRR937575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227404
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92990.4174815165995931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.3412043796275844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.238887961052418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.045.7130551
GTATCAA80650.040.655751
ATCAACG102300.031.9094523
TATCAAC103900.031.6467292
TCAACGC104350.031.2370434
CAACGCA106550.030.5920725
AACGCAG109700.029.7016246
ACGCAGA125250.025.9417697
CGCAGAG126700.025.4574188
GTACATG65500.024.7394681
TACATGG67200.023.6872022
GCAGAGT137150.023.2059969
ACATGGG67300.022.6635633
GAGTACT84300.022.5118112-13
AGTACTT87900.021.61684612-13
AGAGTAC118450.021.6159810-11
GTACTTT90700.020.89432314-15
CAGAGTA127550.020.59519410-11
GTGGTAT24950.020.3793561
CATGGGG48600.020.1404514
Produced by FastQC (version 0.11.5)