##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937575_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227404 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12107547620459 33.0 31.0 34.0 31.0 34.0 2 32.39444573144342 34.0 31.0 34.0 31.0 34.0 3 32.49597064564848 34.0 31.0 34.0 31.0 34.0 4 36.145956009776405 37.0 35.0 37.0 35.0 37.0 5 36.043633754810536 37.0 35.0 37.0 35.0 37.0 6 36.07574198483975 37.0 35.0 37.0 35.0 37.0 7 36.014876959904896 37.0 35.0 37.0 35.0 37.0 8 35.98837346076419 37.0 35.0 37.0 35.0 37.0 9 37.68548139448434 39.0 37.0 39.0 35.0 39.0 10-11 37.59713931554401 39.0 37.0 39.0 35.0 39.0 12-13 37.490246717703656 39.0 37.0 39.0 35.0 39.0 14-15 38.73520609642436 40.0 38.0 41.0 35.0 41.0 16-17 38.68109355105764 40.0 38.0 41.0 34.5 41.0 18-19 38.62408638037823 40.0 38.0 41.0 34.0 41.0 20-21 38.5072752854893 40.0 38.0 41.0 34.0 41.0 22-23 38.44625312695856 40.0 38.0 41.0 34.0 41.0 24-25 38.46264620158714 40.0 38.0 41.0 34.0 41.0 26-27 38.32398612914406 40.0 38.0 41.0 34.0 41.0 28-29 38.16593891364117 40.0 38.0 41.0 33.5 41.0 30-31 38.2306159996121 40.0 38.0 41.0 34.0 41.0 32-33 38.171364961183514 40.0 38.0 41.0 34.0 41.0 34-35 38.03359202012747 40.0 37.0 41.0 33.0 41.0 36-37 37.88606489886882 40.0 37.0 41.0 33.0 41.0 38-39 37.74444555186217 40.0 37.0 41.0 33.0 41.0 40-41 37.55874484377329 40.0 37.0 41.0 33.0 41.0 42-43 37.34329784807785 40.0 36.5 41.0 32.0 41.0 44-45 37.05738765845801 40.0 36.0 41.0 31.5 41.0 46-47 36.707362023234225 39.0 36.0 41.0 31.0 41.0 48-49 36.38444619835468 39.0 35.5 41.0 30.0 41.0 50-51 36.107939781018615 39.0 35.0 40.5 30.0 41.0 52-53 35.844483757773624 39.0 35.0 40.0 29.0 41.0 54-55 35.59144569193555 38.0 35.0 40.0 28.5 41.0 56-57 35.269522053475704 38.0 34.5 40.0 28.0 41.0 58-59 34.96913132956572 38.0 34.0 40.0 27.5 41.0 60-61 34.60591275763176 37.0 34.0 40.0 26.5 41.0 62-63 34.199428796931315 37.0 33.5 39.5 26.0 41.0 64-65 33.75399590734325 36.0 33.0 39.0 26.0 40.5 66-67 33.30110590624781 35.5 32.5 39.0 25.0 40.0 68-69 32.82771446042119 35.0 32.0 38.0 24.0 40.0 70-71 32.423390413234415 35.0 32.0 37.5 23.5 39.5 72-73 31.94422924624361 35.0 31.0 37.0 22.0 39.0 74-75 31.472858987413147 34.5 31.0 36.0 20.5 39.0 76-77 30.26203778030389 33.5 29.5 35.0 20.0 37.0 78-79 30.87647211731684 34.0 31.0 35.0 20.0 37.0 80-81 30.71999623777276 34.0 31.0 35.0 20.0 37.0 82-83 30.386035267962164 34.0 30.5 35.0 18.0 36.5 84-85 30.045997268569153 34.0 30.0 35.0 17.5 36.0 86-87 29.68401197088629 34.0 30.0 35.0 12.0 35.5 88-89 29.266961449292538 34.0 29.0 35.0 7.0 35.0 90-91 28.740552679262496 33.5 29.0 35.0 2.0 35.0 92-93 28.31037880869389 33.0 29.0 35.0 2.0 35.0 94-95 27.835732538865873 33.0 27.0 35.0 2.0 35.0 96-97 27.24453219981647 33.0 26.5 35.0 2.0 35.0 98-99 26.318717664150732 32.5 25.0 34.0 2.0 35.0 100-101 23.506160085911674 29.5 12.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27509985402292614 1101 2 0.2489690673872218 1101 3 0.20877641074866204 1101 4 0.11151649344237313 1101 5 0.07231670122191503 1101 6 0.12309662634370966 1101 7 0.12966857814548405 1101 8 0.12118027262537368 1101 9 0.12998046070005387 1101 10-11 0.17766180972217427 1101 12-13 0.22561559597040315 1101 14-15 0.2951828924113258 1101 16-17 0.23838317461562752 1101 18-19 0.24515863619420486 1101 20-21 0.27869945157336673 1101 22-23 0.2349694249677441 1101 24-25 0.23007177285931135 1101 26-27 0.1269805430838531 1101 28-29 0.11745609074299779 1101 30-31 0.12476903944671136 1101 32-33 0.07653673266727168 1101 34-35 0.2023976677042043 1101 36-37 0.07413348950522902 1101 38-39 0.19823272254079427 1101 40-41 0.3598509539381496 1101 42-43 0.27723929579001094 1101 44-45 0.14827634881349638 1101 46-47 0.0341158286155121 1101 48-49 -0.022742853979458744 1101 50-51 -0.05574893303338513 1101 52-53 -0.09220436025758971 1101 54-55 -0.23775107993039768 1101 56-57 -0.0940000377991197 1101 58-59 -0.20253105964155793 1101 60-61 -0.11729653648630034 1101 62-63 -0.29472291137994944 1101 64-65 -0.1783561375421172 1101 66-67 -0.3023324992589309 1101 68-69 -0.3090844505262922 1101 70-71 -0.2519801035027669 1101 72-73 -0.36003457437935893 1101 74-75 -0.3114695701205896 1101 76-77 -0.2948936272493867 1101 78-79 -0.3065559312734685 1101 80-81 -0.3541897383563999 1101 82-83 -0.34316858586981525 1101 84-85 -0.3846866919435321 1101 86-87 -0.4818409077970891 1101 88-89 -0.4806829780268487 1101 90-91 -0.4690899287156505 1101 92-93 -0.7715054734904463 1101 94-95 -0.8224411221603809 1101 96-97 -0.6866281745204716 1101 98-99 -0.8951661435345315 1101 100-101 -0.7308989253018439 1102 1 0.26463609713405134 1102 2 0.22777890955318014 1102 3 0.17144337101070306 1102 4 0.13742893834901793 1102 5 0.045936449225109754 1102 6 0.13028308063125849 1102 7 0.08584504665295611 1102 8 0.15874058348273934 1102 9 0.24241068758206552 1102 10-11 0.20813559483865163 1102 12-13 0.3022576961324077 1102 14-15 0.2951413292105016 1102 16-17 0.27445494807319193 1102 18-19 0.271550163314636 1102 20-21 0.31292630529211607 1102 22-23 0.26771149966123886 1102 24-25 0.26350693603466624 1102 26-27 0.20250286871296197 1102 28-29 0.22154029425058042 1102 30-31 0.3152943098728116 1102 32-33 0.358345625069866 1102 34-35 0.3237896281431105 1102 36-37 0.2229987256903243 1102 38-39 0.3452941600262065 1102 40-41 0.4191780015005975 1102 42-43 0.4910266938275214 1102 44-45 0.5310774078792235 1102 46-47 0.37296977199051895 1102 48-49 0.33917733936901584 1102 50-51 0.459126934487891 1102 52-53 0.6021750127217302 1102 54-55 0.6324505811827237 1102 56-57 0.4549742354638866 1102 58-59 0.4282915468486692 1102 60-61 0.4496102682055678 1102 62-63 0.3456200999889134 1102 64-65 0.29844982774995543 1102 66-67 0.2588076323991473 1102 68-69 0.26965725452000555 1102 70-71 0.1551517880296558 1102 72-73 0.22549453401039088 1102 74-75 0.014054745236215638 1102 76-77 -0.04025026604217885 1102 78-79 0.11964993214454367 1102 80-81 0.1268740231309593 1102 82-83 0.28657625145005383 1102 84-85 0.26647866946176535 1102 86-87 0.07771318980391584 1102 88-89 0.01990752893808434 1102 90-91 0.15649770419815923 1102 92-93 0.02299169136866297 1102 94-95 -0.2239025108311381 1102 96-97 -0.2765654140872229 1102 98-99 0.02500045415490959 1102 100-101 -0.17038843192011655 1103 1 0.295361686213262 1103 2 0.24220277607685858 1103 3 0.15886354703404493 1103 4 0.12274543256691572 1103 5 0.0779505736007664 1103 6 0.09969153786686036 1103 7 0.13168647107016085 1103 8 0.1309857505696641 1103 9 0.11889968853850519 1103 10-11 0.1906775718648035 1103 12-13 0.23266411006742516 1103 14-15 0.2497714821440553 1103 16-17 0.21899358722534856 1103 18-19 0.11723145672873869 1103 20-21 0.100773859611067 1103 22-23 0.1734642602907499 1103 24-25 0.07312856313955507 1103 26-27 0.17058837335506638 1103 28-29 0.07776233774438168 1103 30-31 0.20782720556304213 1103 32-33 0.24982064173508434 1103 34-35 0.17406955549242298 1103 36-37 0.17609881843588227 1103 38-39 0.0948183559898439 1103 40-41 0.03796175460419704 1103 42-43 0.03289080709303249 1103 44-45 0.14418411840680534 1103 46-47 -0.020844413249413662 1103 48-49 -0.08415541430457552 1103 50-51 -0.152412883305729 1103 52-53 0.0073312068691038235 1103 54-55 -0.15354991152806008 1103 56-57 -0.11168482316869444 1103 58-59 -0.08955110451497461 1103 60-61 -0.16612725220328173 1103 62-63 -0.1408303535948363 1103 64-65 -0.12843092658058453 1103 66-67 -0.12687018305651776 1103 68-69 -0.009265955055973052 1103 70-71 -0.30921664853141095 1103 72-73 -0.43916807986859396 1103 74-75 -0.2986143221878699 1103 76-77 -0.22022012373571087 1103 78-79 -0.11261066838294553 1103 80-81 -0.23876326444789697 1103 82-83 -0.14288036584892794 1103 84-85 -0.232124109040587 1103 86-87 -0.24760393237869494 1103 88-89 -0.19211811617490326 1103 90-91 -0.09794756143091732 1103 92-93 -0.2182844295483619 1103 94-95 -0.5313486024753438 1103 96-97 -0.4886550632964699 1103 98-99 -0.4112255020421358 1103 100-101 -0.3991469215200567 1104 1 0.24204627402687606 1104 2 0.19964005206252722 1104 3 0.18440118144264517 1104 4 0.10706442898268875 1104 5 0.10707243740004913 1104 6 0.12298902890628938 1104 7 0.12675815565797421 1104 8 0.09738360003202473 1104 9 0.13726886416574757 1104 10-11 0.17790875466050693 1104 12-13 0.13476984483445165 1104 14-15 0.2383467509612629 1104 16-17 0.24027494923251425 1104 18-19 0.19608450332371774 1104 20-21 0.0709799528010322 1104 22-23 0.1382940093946914 1104 24-25 0.015332864214819608 1104 26-27 0.1764127461149272 1104 28-29 0.06768169258309342 1104 30-31 0.13591860341250595 1104 32-33 0.13646938725479885 1104 34-35 0.22245665226661515 1104 36-37 0.22650204424233777 1104 38-39 0.07196889362425196 1104 40-41 0.13271981912031805 1104 42-43 0.18880478558766356 1104 44-45 -0.015986132489246074 1104 46-47 -0.10574584694116851 1104 48-49 0.0034341914301876386 1104 50-51 0.1096838908878155 1104 52-53 0.22708927138523194 1104 54-55 0.013340769475526315 1104 56-57 0.16452485425065788 1104 58-59 0.20301161949935675 1104 60-61 0.20551253274295078 1104 62-63 0.16091695823312335 1104 64-65 0.07475007700364955 1104 66-67 0.11618537249903937 1104 68-69 0.16255304135979287 1104 70-71 0.05615073498113077 1104 72-73 0.15155772658301814 1104 74-75 0.09318675308094626 1104 76-77 0.030003890601207672 1104 78-79 -0.004187729314843125 1104 80-81 0.057159674620201883 1104 82-83 0.032298845620605476 1104 84-85 0.17222176909561426 1104 86-87 0.08550538661771867 1104 88-89 -0.06779015918565534 1104 90-91 0.21589652459058684 1104 92-93 0.11885714751257126 1104 94-95 0.054224874227163156 1104 96-97 0.2824022843737808 1104 98-99 0.5197780821872513 1104 100-101 0.4572598885157859 1105 1 0.32712051877854265 1105 2 0.25981670336820883 1105 3 0.1922096811866325 1105 4 0.10865173056998856 1105 5 0.07362389669344793 1105 6 0.10122763617715691 1105 7 0.14453849359959747 1105 8 0.17710710233617277 1105 9 0.2281162784002575 1105 10-11 0.23180020371324161 1105 12-13 0.16544060776327996 1105 14-15 0.20112196857518683 1105 16-17 0.22746772957045636 1105 18-19 0.2806595161245369 1105 20-21 0.06287703421424595 1105 22-23 0.020795289476616574 1105 24-25 0.12908094409193893 1105 26-27 0.18691581831154735 1105 28-29 0.22154753999733146 1105 30-31 0.14636407192249123 1105 32-33 0.16462478920052348 1105 34-35 0.2654034008585313 1105 36-37 0.1717785419381883 1105 38-39 0.1348081153344438 1105 40-41 0.23522877969379863 1105 42-43 0.18628942460455278 1105 44-45 0.20854023719841308 1105 46-47 0.24859363078540042 1105 48-49 0.21423168246961666 1105 50-51 0.3129352989779406 1105 52-53 0.28955726933709514 1105 54-55 0.1474869548313933 1105 56-57 0.16348798789121588 1105 58-59 0.2774611842715089 1105 60-61 0.2632250263425462 1105 62-63 0.09350144364018576 1105 64-65 0.19650506932315892 1105 66-67 0.3134651984078971 1105 68-69 0.27182083449856265 1105 70-71 0.13505498485312017 1105 72-73 0.21677790067416325 1105 74-75 0.2075364714629231 1105 76-77 0.12801336320745094 1105 78-79 0.31440546064931496 1105 80-81 0.35642362751318757 1105 82-83 0.3562875808996644 1105 84-85 0.48401772403672894 1105 86-87 0.3039833692086056 1105 88-89 0.4432953502869523 1105 90-91 0.6088496735921254 1105 92-93 0.7017616534009505 1105 94-95 0.658455544990094 1105 96-97 0.5429821614859165 1105 98-99 0.5227542726634447 1105 100-101 0.44274375948352684 1106 1 0.01789696423931275 1106 2 0.054414435204911626 1106 3 0.06504859980437772 1106 4 -0.020133281444081774 1106 5 -0.05841129814340462 1106 6 -0.15262557163721624 1106 7 -0.00816438165141875 1106 8 -0.07949134918386846 1106 9 -0.02861798433059448 1106 10-11 -0.13620696990455627 1106 12-13 -0.32941946025839997 1106 14-15 -0.11132507085649479 1106 16-17 0.043248527954652616 1106 18-19 -0.04609181006613028 1106 20-21 0.08537352285601685 1106 22-23 0.07835138414979781 1106 24-25 0.05203013676973711 1106 26-27 0.05457591883505586 1106 28-29 0.07211864355047481 1106 30-31 0.12649036474073938 1106 32-33 0.01988137952658775 1106 34-35 -0.29617479505785127 1106 36-37 -0.09244855297875887 1106 38-39 0.04205660341903439 1106 40-41 -0.2702599665481813 1106 42-43 -0.2763711982697714 1106 44-45 -0.16896095260065636 1106 46-47 -0.18556288873310933 1106 48-49 -0.024612064991181626 1106 50-51 0.03285543734286023 1106 52-53 0.20650929431707254 1106 54-55 0.07645537001902625 1106 56-57 -0.07170859013058362 1106 58-59 -0.04783974783450162 1106 60-61 0.25369096326577534 1106 62-63 0.03647118211654998 1106 64-65 -0.0874628235136683 1106 66-67 -0.25637078343751085 1106 68-69 0.08092018562277303 1106 70-71 0.02084065851155259 1106 72-73 0.10798789730789693 1106 74-75 -0.016638504450902758 1106 76-77 -0.1634705774070646 1106 78-79 -0.21097440446810012 1106 80-81 -0.2600542190085342 1106 82-83 -0.3111258539631905 1106 84-85 -0.451622271784462 1106 86-87 -0.0920194863091588 1106 88-89 -0.26878778474653586 1106 90-91 -0.30356428362153665 1106 92-93 -0.34201076415467924 1106 94-95 -0.16349595763907132 1106 96-97 -0.239867900615792 1106 98-99 -0.4202827910285336 1106 100-101 -0.6465062608301153 1107 1 0.16492560497072617 1107 2 0.14358071315372456 1107 3 0.06656366650835821 1107 4 -0.027798175557336435 1107 5 0.02926479104402091 1107 6 0.03343904881867843 1107 7 0.10601542367868433 1107 8 0.08752298673055492 1107 9 -0.028125401067228495 1107 10-11 0.0996769744932422 1107 12-13 0.07109800094755059 1107 14-15 0.12595328222371194 1107 16-17 0.13566821884621305 1107 18-19 0.20573205410030226 1107 20-21 0.17209305515068962 1107 22-23 0.1395903056909873 1107 24-25 0.10015962646094323 1107 26-27 0.17277973137976232 1107 28-29 0.10293059740189392 1107 30-31 0.14580311157658343 1107 32-33 0.18260738008633837 1107 34-35 0.22446581196394533 1107 36-37 0.22388754802569366 1107 38-39 0.14051728072102065 1107 40-41 0.1709316869020796 1107 42-43 0.2591681866230999 1107 44-45 0.19887248957367376 1107 46-47 0.24508051705723233 1107 48-49 0.13038062711318332 1107 50-51 0.27927767822354355 1107 52-53 0.36916812443182323 1107 54-55 0.3622426014149909 1107 56-57 0.4208908438962169 1107 58-59 0.4625536346327763 1107 60-61 0.4026600835195424 1107 62-63 0.3170390211562619 1107 64-65 0.5011548958009442 1107 66-67 0.37567262857231754 1107 68-69 0.5359023046015352 1107 70-71 0.45453783175532436 1107 72-73 0.3935947636200616 1107 74-75 0.3362233918702735 1107 76-77 0.3461478571483987 1107 78-79 0.2514225853008476 1107 80-81 0.4093092166353358 1107 82-83 0.4364923940077041 1107 84-85 0.5541463011545567 1107 86-87 0.4045585526870177 1107 88-89 0.4684663123396362 1107 90-91 0.5908835815400089 1107 92-93 0.5795092781378202 1107 94-95 0.39837361974687724 1107 96-97 0.46448014180110064 1107 98-99 0.544376847619354 1107 100-101 0.5624410912238673 1108 1 0.21527461136150094 1108 2 0.1956452225495795 1108 3 0.11084382931661452 1108 4 0.06659525317721204 1108 5 0.1023811694811343 1108 6 0.08696048339762541 1108 7 0.1024717042589458 1108 8 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-0.4841998179401017 2316 54-55 -0.36687987612714323 2316 56-57 -0.3608180556347236 2316 58-59 -0.4975666474264173 2316 60-61 -0.39304866398203586 2316 62-63 -0.5131095651941848 2316 64-65 -0.5585069729977121 2316 66-67 -0.2910591424356781 2316 68-69 -0.34375473502894494 2316 70-71 -0.18184190183556126 2316 72-73 -0.3529444666641943 2316 74-75 -0.2126980491892816 2316 76-77 -0.23360451383903325 2316 78-79 -0.292189274778476 2316 80-81 -0.3024871892115364 2316 82-83 -0.24615559987535107 2316 84-85 -0.3051469411400163 2316 86-87 -0.3905460122183477 2316 88-89 -0.2802592791216796 2316 90-91 -0.07492089974919836 2316 92-93 0.08063134430636865 2316 94-95 0.16267077456196333 2316 96-97 -0.06257241117442547 2316 98-99 -0.02019083004261546 2316 100-101 0.12741504501588352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 13.0 5 38.0 6 103.0 7 244.0 8 536.0 9 1046.0 10 1843.0 11 2882.0 12 4376.0 13 6100.0 14 7818.0 15 10855.0 16 14273.0 17 19667.0 18 18078.0 19 12014.0 20 11144.0 21 11215.0 22 11757.0 23 12975.0 24 14978.0 25 18370.0 26 22068.0 27 27728.0 28 34775.0 29 43732.0 30 55239.0 31 69778.0 32 89497.0 33 118335.0 34 163796.0 35 238298.0 36 363619.0 37 504108.0 38 300288.0 39 15807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84796363308172 18.594401006879416 14.942936465208465 23.6146988948304 2 16.42894598375508 19.763590260231194 38.42827794149602 25.379185814517708 3 19.466024124945452 22.422156016600493 30.03388698233459 28.077932876119466 4 14.081639433169734 16.04540532386581 34.74367469933608 35.12928054362837 5 14.838317915256194 36.24897204085877 33.47883830174637 15.433871742138667 6 34.69074312518071 34.43299913262255 15.958712474252538 14.917545267944208 7 29.997342197463954 32.12982467482325 19.375066220586838 18.497766907125964 8 27.38564714798034 34.35748521597339 18.859847607349185 19.397020028697085 9 26.582200624583596 16.092096449499056 19.66513483858339 37.66056808733396 10-11 25.975664944482457 25.717516894106325 26.23190494405146 22.07491321735976 12-13 26.269355168921106 22.736554196268866 27.42155117917921 23.57253945563082 14-15 23.216713268001673 24.47034754359784 25.22247872411112 27.090460464289368 16-17 23.259992349838647 27.642515681932867 26.048125979840208 23.049365988388278 18-19 23.14530727250198 25.937324347087458 28.332534196759994 22.584834183650564 20-21 23.46493945418074 25.17776299225466 28.655511079265366 22.70178647429923 22-23 23.48907429319006 24.8129588762066 28.705865556665227 22.992101273938108 24-25 23.49049664036017 25.00598793254816 28.60386554436985 22.899649882721825 26-27 23.153030376836554 25.538151134644583 28.460586839689743 22.848231648829124 28-29 23.032148113162652 25.6816085998874 28.523088582180485 22.763154704769466 30-31 23.278848381344382 25.184991137665193 28.499679447464406 23.036481033526023 32-33 23.28749072911784 25.10283271467592 28.47263002131629 23.137046534889944 34-35 23.744502568909816 25.172622478903694 28.174906752434676 22.907968199751817 36-37 23.73568063988392 25.191029557278338 28.07519426201982 22.99809554081792 38-39 23.287243804895745 25.282795577272914 28.235739901697222 23.19422071613412 40-41 23.56555141468329 25.219483440027762 28.094778235862705 23.120186909426238 42-43 23.481354098147197 25.26615675084693 27.870117391392196 23.38237175961368 44-45 23.41712594571977 25.40610055472649 27.872110313171746 23.304663186381994 46-47 23.70122348707284 25.739740074095224 27.31801235878179 23.24102408005014 48-49 23.94258069034625 25.630554672614398 27.098137562830992 23.32872707420836 50-51 23.73437867580376 25.95407029887708 27.024150086827536 23.287400938491626 52-53 24.01989581142348 25.928575581388824 26.73336914483613 23.318159462351566 54-55 23.759192888931988 25.875897514637014 26.791583554106335 23.573326042324663 56-57 23.696554374509518 26.193990852130998 26.87987899815211 23.229575775207373 58-59 23.964534498456498 25.76198570174068 26.805622150270004 23.46785764953282 60-61 23.660503438083076 25.823245356477763 27.28681011617111 23.229441089268043 62-63 23.485344373988735 25.92044370935852 27.30351117264762 23.29070074400513 64-65 23.566727709354794 25.9077724147808 27.069528953673856 23.45597092219055 66-67 23.54530206464566 25.9095117006165 26.94906267565291 23.596123559084926 68-69 23.39283758132786 25.69327342502752 27.42966700248361 23.48422199116101 70-71 23.362510797322802 25.936179516603186 27.096835598750836 23.60447408732318 72-73 23.564629496939038 25.938401834602075 26.71821097564699 23.7787576928119 74-75 23.46119069553615 26.29920301840169 26.531558711396762 23.708047574665393 76-77 23.667439763958402 25.998741135420428 26.746719499471133 23.587099601150037 78-79 23.644632944899087 26.03988768988473 26.76380216610907 23.55167719910712 80-81 23.525188964372877 26.233633413606157 26.693406315154323 23.54777130686665 82-83 23.621250966096873 26.202747115963724 26.65080505641018 23.52519686152922 84-85 23.532921523406404 26.076129914523776 26.526362764169754 23.864585797900066 86-87 23.576481859599784 25.889533286283044 26.75861675744499 23.77536809667218 88-89 23.597290837225756 26.1079040892447 26.56446697590558 23.73033809762396 90-91 23.53790456485565 26.016906839515684 26.59628062102799 23.848907974600678 92-93 23.319187336620473 25.68891303298625 26.81654464294734 24.175354987445942 94-95 23.357482522254607 25.782906917649424 26.793141253225727 24.06646930687024 96-97 23.491248107662553 25.633517763279585 26.706291270016575 24.168942859041287 98-99 23.230776137830407 25.78387114877378 26.533924462652227 24.451428250743586 100-101 23.262952001584186 25.58166529038249 26.838506859642813 24.31687584839051 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 336.0 1 273.0 2 278.0 3 376.0 4 321.0 5 308.5 6 613.0 7 1413.5 8 2375.0 9 2744.5 10 2427.0 11 2104.5 12 2237.5 13 2504.5 14 2744.0 15 2893.5 16 3004.5 17 3202.5 18 3414.5 19 3589.0 20 3844.0 21 4301.0 22 5033.0 23 5915.0 24 6801.0 25 8313.0 26 10129.5 27 12207.5 28 14440.0 29 16689.0 30 18921.0 31 21497.0 32 24139.5 33 25293.5 34 26452.5 35 28287.0 36 31459.0 37 36122.5 38 41583.0 39 45381.0 40 48581.5 41 54902.5 42 60343.0 43 64648.0 44 70863.5 45 77211.0 46 81557.0 47 86071.0 48 90237.5 49 91504.5 50 91846.5 51 90966.0 52 91264.0 53 93638.5 54 96239.5 55 92054.5 56 82065.0 57 75086.5 58 69708.0 59 62071.0 60 51657.5 61 42098.5 62 34253.5 63 26781.0 64 20444.0 65 15572.0 66 11389.5 67 9008.5 68 6528.0 69 4082.5 70 2962.0 71 2067.0 72 1576.5 73 1221.5 74 950.0 75 776.0 76 509.0 77 300.0 78 204.5 79 133.5 80 73.0 81 54.5 82 41.0 83 25.0 84 18.5 85 9.0 86 3.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014590976760390123 2 0.0 3 0.0 4 0.0 5 0.04063025836354788 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004130368806018127 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.754586056234073E-4 24-25 3.367148483166951E-4 26-27 0.0014590976760390122 28-29 0.0011672781408312097 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.734296966333902E-4 42-43 0.01072997983302535 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2447656554446342E-5 54-55 1.3468593932667805E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.4895313108892685E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.060867269700511E-4 84-85 4.4895313108892685E-5 86-87 0.0 88-89 0.0 90-91 0.011582990782094313 92-93 0.001638678928474583 94-95 0.0 96-97 0.0 98-99 0.0013019640801578878 100-101 0.017643858051794824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2227404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.705107486010775 #Duplication Level Percentage of deduplicated Percentage of total 1 62.860056676037516 23.701451935467237 2 16.16259334207783 12.188246384314533 3 7.310041042345519 8.26877649686364 4 3.9783011698024526 6.000090928764956 5 2.3736597982390375 4.474954891391278 6 1.555646574551062 3.5193492782215356 7 1.0956096324804148 2.8917055268767955 8 0.779908255771866 2.3525219690484307 9 0.5765521929521651 1.9565066165900948 >10 2.9978926598129356 20.060626003551032 >50 0.20267730189097607 5.210488888217241 >100 0.10108254168124899 6.748640356051992 >500 0.005022202379888288 1.293392194510288 >1k 5.978812357009845E-4 0.331429777315338 >5k 3.587287414205907E-4 1.0018187528155096 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9299 0.4174815165995931 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7600 0.3412043796275844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.238887961052418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.2447656554446342E-5 0.0 18-19 0.0 0.0 0.0 4.4895313108892685E-5 0.0 20-21 0.0 0.0 0.0 6.734296966333902E-5 0.0 22-23 0.0 0.0 0.0 1.3468593932667805E-4 0.0 24-25 0.0 0.0 0.0 2.0202890899001708E-4 0.0 26-27 0.0 0.0 0.0 2.9181953520780247E-4 0.0 28-29 0.0 0.0 0.0 0.0012346211104945488 0.0 30-31 0.0 0.0 0.0 0.003479386765939183 0.0 32-33 0.0 0.0 0.0 0.0068016399359972415 0.0 34-35 0.0 0.0 0.0 0.010078997792946407 0.0 36-37 0.0 0.0 0.0 0.014523633790726784 0.0 38-39 0.0 0.0 0.0 0.019484565889259425 0.0 40-41 0.0 0.0 0.0 0.02583725269416774 0.0 42-43 0.0 0.0 0.0 0.03362658951856062 0.0 44-45 0.0 0.0 0.0 0.042066908383032446 0.0 46-47 0.0 0.0 0.0 0.05203366789320662 0.0 48-49 0.0 0.0 0.0 0.06330239148353868 0.0 50-51 0.0 0.0 0.0 0.07713014792107764 0.0 52-53 0.0 0.0 0.0 0.11309129372130067 2.2447656554446342E-5 54-55 0.0 0.0 0.0 0.16539433349316066 4.4895313108892685E-5 56-57 0.0 0.0 0.0 0.20606948716981743 4.4895313108892685E-5 58-59 0.0 0.0 0.0 0.26524150984733796 4.4895313108892685E-5 60-61 0.0 0.0 0.0 0.33294364201554816 4.4895313108892685E-5 62-63 0.0 0.0 0.0 0.38230603877877567 4.4895313108892685E-5 64-65 0.0 0.0 0.0 0.42589938780751047 4.4895313108892685E-5 66-67 0.0 0.0 0.0 0.5381152229231877 4.4895313108892685E-5 68-69 0.0 0.0 0.0 0.6508024588265083 4.4895313108892685E-5 70-71 0.0 0.0 0.0 0.7136334495224037 4.4895313108892685E-5 72-73 0.0 0.0 0.0 0.8323142097257614 4.4895313108892685E-5 74-75 0.0 0.0 0.0 1.0021082839035935 4.4895313108892685E-5 76-77 0.0 0.0 0.0 1.175583773756355 4.4895313108892685E-5 78-79 0.0 0.0 0.0 1.3519774589611941 4.4895313108892685E-5 80-81 0.0 0.0 0.0 1.5670260087527903 4.4895313108892685E-5 82-83 0.0 0.0 0.0 1.8018958392819624 4.4895313108892685E-5 84-85 0.0 0.0 0.0 2.049403700451288 4.4895313108892685E-5 86-87 0.0 0.0 0.0 2.3851532995361415 4.4895313108892685E-5 88-89 0.0 0.0 0.0 2.7755180470179637 4.4895313108892685E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4865 0.0 45.713055 1 GTATCAA 8065 0.0 40.65575 1 ATCAACG 10230 0.0 31.909452 3 TATCAAC 10390 0.0 31.646729 2 TCAACGC 10435 0.0 31.237043 4 CAACGCA 10655 0.0 30.592072 5 AACGCAG 10970 0.0 29.701624 6 ACGCAGA 12525 0.0 25.941769 7 CGCAGAG 12670 0.0 25.457418 8 GTACATG 6550 0.0 24.739468 1 TACATGG 6720 0.0 23.687202 2 GCAGAGT 13715 0.0 23.205996 9 ACATGGG 6730 0.0 22.663563 3 GAGTACT 8430 0.0 22.51181 12-13 AGTACTT 8790 0.0 21.616846 12-13 AGAGTAC 11845 0.0 21.61598 10-11 GTACTTT 9070 0.0 20.894323 14-15 CAGAGTA 12755 0.0 20.595194 10-11 GTGGTAT 2495 0.0 20.379356 1 CATGGGG 4860 0.0 20.140451 4 >>END_MODULE