FastQCFastQC Report
Thu 26 May 2016
SRR937574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260135
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69890.3092293159479412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56290.2490559192260639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35980.15919403044508404No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31330.13862003818355983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40400.046.2607541
GTATCAA65250.039.6478731
ATCAACG83100.030.3713193
TATCAAC84550.030.0211072
TCAACGC85050.029.6795854
CAACGCA86650.029.1321955
AACGCAG87550.028.7611876
GTACATG60400.025.9823531
TACATGG63300.024.6302722
ACGCAGA102250.024.3475387
CGCAGAG103100.024.1928888
ACATGGG63950.024.1553293
GTCGTAA1652.4070232E-623.057251
GAGTACT68000.021.62030412-13
GCAGAGT112650.021.3827659
CATGGGG45550.021.1858184
AGTACTT71600.020.69910812-13
AGAGTAC98100.020.53084810-11
CCGATCG2801.5734258E-920.3615259
CAGAGTA104350.019.75637610-11
Produced by FastQC (version 0.11.5)