##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937574_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260135 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64537516564276 33.0 31.0 34.0 30.0 34.0 2 31.77293878463012 33.0 31.0 34.0 30.0 34.0 3 31.831741024319342 33.0 31.0 34.0 30.0 34.0 4 35.42843281485398 37.0 35.0 37.0 33.0 37.0 5 35.39554362903101 37.0 35.0 37.0 33.0 37.0 6 35.48772971526037 37.0 35.0 37.0 33.0 37.0 7 35.413965979908276 37.0 35.0 37.0 33.0 37.0 8 35.40285425428127 37.0 35.0 37.0 33.0 37.0 9 37.00963968966455 39.0 37.0 39.0 33.0 39.0 10-11 36.8991794737925 39.0 37.0 39.0 33.0 39.0 12-13 36.765878586898566 39.0 37.0 39.0 33.0 39.0 14-15 37.91714831193712 40.0 38.0 41.0 33.0 41.0 16-17 37.84188488740717 40.0 38.0 41.0 33.0 41.0 18-19 37.754155172146795 40.0 37.5 41.0 32.5 41.0 20-21 37.56748778280944 40.0 37.0 41.0 32.0 41.0 22-23 37.42319728688773 40.0 37.0 41.0 32.0 41.0 24-25 37.265552721408234 39.0 37.0 41.0 31.5 41.0 26-27 37.362586305685284 39.5 37.0 41.0 32.0 41.0 28-29 37.29815099540514 39.5 37.0 41.0 31.5 41.0 30-31 37.10770772542348 39.0 36.5 41.0 31.0 41.0 32-33 36.884313768867784 39.0 36.0 41.0 30.5 41.0 34-35 36.61640919679577 39.0 35.5 40.0 30.0 41.0 36-37 36.66337032964845 39.0 36.0 41.0 30.0 41.0 38-39 36.624212270505964 39.0 36.0 41.0 30.0 41.0 40-41 36.43460722478967 39.0 35.0 41.0 30.0 41.0 42-43 36.19744506412228 39.0 35.0 41.0 30.0 41.0 44-45 35.98011778057506 39.0 35.0 40.0 29.0 41.0 46-47 35.64252998161614 39.0 35.0 40.0 27.5 41.0 48-49 35.31764916697453 38.0 35.0 40.0 26.5 41.0 50-51 34.38841639990532 37.5 33.5 39.5 25.0 40.5 52-53 34.47224236605336 38.0 33.5 39.5 26.0 40.5 54-55 34.75140843356702 38.0 34.0 40.0 26.0 41.0 56-57 34.44212558099406 38.0 34.0 40.0 24.5 41.0 58-59 34.16998984573931 37.5 33.5 40.0 24.0 41.0 60-61 33.834759870538704 37.0 33.0 40.0 23.0 41.0 62-63 33.43810303366834 36.5 33.0 39.5 22.5 41.0 64-65 33.031894554971274 36.0 32.0 39.0 22.0 41.0 66-67 32.584607114176805 35.5 32.0 39.0 20.5 40.0 68-69 32.083850300977595 35.0 31.0 38.0 18.5 40.0 70-71 31.622397777123933 35.0 31.0 37.5 18.0 40.0 72-73 31.15217807785818 35.0 30.5 37.0 14.5 39.0 74-75 30.658594287509374 34.5 30.0 36.0 9.5 39.0 76-77 30.20070349779991 34.0 30.0 36.0 7.5 38.5 78-79 29.683300333829614 34.0 29.0 35.0 4.5 37.0 80-81 29.181847986956534 34.0 29.0 35.0 2.0 37.0 82-83 28.68748747309342 34.0 28.5 35.0 2.0 36.0 84-85 28.235593449063884 33.5 27.0 35.0 2.0 36.0 86-87 27.71801286206355 33.0 26.5 35.0 2.0 35.5 88-89 27.242784391197873 33.0 25.5 35.0 2.0 35.0 90-91 26.730112360544837 32.5 25.0 35.0 2.0 35.0 92-93 26.147650693432027 32.0 24.0 34.0 2.0 35.0 94-95 25.485859030544635 32.0 21.5 34.0 2.0 35.0 96-97 24.659759040942244 31.5 18.0 34.0 2.0 35.0 98-99 23.673815059719885 31.0 2.0 34.0 2.0 35.0 100-101 21.113924168246587 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27467477817547703 1101 2 0.36906994776879287 1101 3 0.2906228555841466 1101 4 0.3634517846434733 1101 5 0.35228011567750883 1101 6 0.32293778531231254 1101 7 0.2704301746327573 1101 8 0.34760744032040236 1101 9 0.3802373643678152 1101 10-11 0.2896377584261316 1101 12-13 0.3793936138827334 1101 14-15 0.5393684889619692 1101 16-17 0.6283710403377043 1101 18-19 0.5344231099504029 1101 20-21 0.5573707215829131 1101 22-23 0.599338149213196 1101 24-25 0.6575560704773835 1101 26-27 0.6187004888436292 1101 28-29 0.5789776745698774 1101 30-31 0.48275621738489605 1101 32-33 0.4335747658932476 1101 34-35 0.7166488158439321 1101 36-37 0.6137312499175138 1101 38-39 0.7000388626678031 1101 40-41 0.8761121870674629 1101 42-43 0.6473009505663967 1101 44-45 0.8266755780299562 1101 46-47 1.0096811333366347 1101 48-49 0.9235567637350002 1101 50-51 0.8905141490785056 1101 52-53 0.8848791946595611 1101 54-55 0.9677900022057102 1101 56-57 1.1249457768123605 1101 58-59 1.0820195309881555 1101 60-61 0.9764594656961023 1101 62-63 0.8251986566781042 1101 64-65 0.938880519913738 1101 66-67 0.9006069419735212 1101 68-69 0.9941524131416983 1101 70-71 0.9643779704781537 1101 72-73 1.0333462104238968 1101 74-75 1.087787694170192 1101 76-77 1.130766147960859 1101 78-79 1.0887791075053563 1101 80-81 1.2605936716633686 1101 82-83 1.273538585204836 1101 84-85 1.2619212379237545 1101 86-87 1.516497519503627 1101 88-89 1.4792139691725978 1101 90-91 1.543121601785522 1101 92-93 1.7221125427302901 1101 94-95 1.6688699977024655 1101 96-97 1.8711863315860668 1101 98-99 2.275151851127003 1101 100-101 2.4666344846352253 1102 1 0.2525727061132521 1102 2 0.2494816494303791 1102 3 0.27869034622153777 1102 4 0.27606894495333023 1102 5 0.28139443744451853 1102 6 0.23703500345971662 1102 7 0.2372047039903009 1102 8 0.15611120214188645 1102 9 0.2656130244183075 1102 10-11 0.2782647465449912 1102 12-13 0.3616122046464554 1102 14-15 0.4905717600491357 1102 16-17 0.5434479649143782 1102 18-19 0.5403064101550683 1102 20-21 0.4055125694262145 1102 22-23 0.6445121577103592 1102 24-25 0.7467828428958612 1102 26-27 0.6676980894439737 1102 28-29 0.5426078074601364 1102 30-31 0.5343560577373623 1102 32-33 0.43063133484735516 1102 34-35 0.7399747710717577 1102 36-37 0.7975283753246245 1102 38-39 0.6858412742903539 1102 40-41 0.6675267642967668 1102 42-43 0.7054863452919236 1102 44-45 0.6446652076893074 1102 46-47 0.7035760212974793 1102 48-49 0.6650727703871055 1102 50-51 0.3201002334228633 1102 52-53 0.43305671761451947 1102 54-55 0.5865324460839574 1102 56-57 0.6716246826231256 1102 58-59 0.6168975297924888 1102 60-61 0.7130188252013028 1102 62-63 0.7094082673619653 1102 64-65 0.6968381038369529 1102 66-67 0.6769232420501368 1102 68-69 0.735997238216477 1102 70-71 0.777193771609376 1102 72-73 0.7658993842434292 1102 74-75 0.5325818180312538 1102 76-77 0.8377497525943873 1102 78-79 0.8703434733366642 1102 80-81 0.7864257894246087 1102 82-83 0.8591526667319336 1102 84-85 1.0227596386664253 1102 86-87 1.0628757270532887 1102 88-89 1.159933624096972 1102 90-91 1.2633166207300768 1102 92-93 1.2404034892942981 1102 94-95 1.2926155807280857 1102 96-97 1.47583794996698 1102 98-99 1.6938328430788268 1102 100-101 1.923406578786775 1103 1 0.2554054838823454 1103 2 0.2920572959031986 1103 3 0.18534264783529864 1103 4 0.2640205506969764 1103 5 0.19338673601173895 1103 6 0.2636037468544785 1103 7 0.22515321956312562 1103 8 0.3218801438892527 1103 9 0.29013607472956693 1103 10-11 0.3620286436793805 1103 12-13 0.35722143545931573 1103 14-15 0.5296596943135725 1103 16-17 0.4463654315414374 1103 18-19 0.38789000563788534 1103 20-21 0.2812433493816897 1103 22-23 0.26116601531601447 1103 24-25 0.3316737435061512 1103 26-27 0.09959345117028562 1103 28-29 0.3103518409764874 1103 30-31 0.33091666125863384 1103 32-33 0.3016754163158879 1103 34-35 0.2858214542459976 1103 36-37 0.3315466116950532 1103 38-39 0.5914567327062912 1103 40-41 0.6185729143620051 1103 42-43 0.5972886492505793 1103 44-45 0.48420836047062465 1103 46-47 0.40123852520108727 1103 48-49 0.3018287345076729 1103 50-51 0.14844151697803198 1103 52-53 0.12434883437062894 1103 54-55 0.1756573351559112 1103 56-57 0.29920183964416225 1103 58-59 0.32392188797309274 1103 60-61 0.2675516150038888 1103 62-63 0.21419994076944704 1103 64-65 0.29041665616099976 1103 66-67 0.4664340752801621 1103 68-69 0.5793938340115901 1103 70-71 0.6534132529914558 1103 72-73 0.5235735508152679 1103 74-75 0.6178429029284267 1103 76-77 0.44407707730263013 1103 78-79 0.5834922774223763 1103 80-81 0.6904362676485398 1103 82-83 0.5597002086464684 1103 84-85 0.6570261066411689 1103 86-87 0.6899964476665836 1103 88-89 0.6331398435565418 1103 90-91 0.6813780147897468 1103 92-93 0.9802701829605738 1103 94-95 0.8678887790948764 1103 96-97 1.0125982585041768 1103 98-99 1.0514195941713815 1103 100-101 1.5275477894139122 1104 1 0.166423302250859 1104 2 0.23980309264933908 1104 3 0.2788061658271701 1104 4 0.35589456024974453 1104 5 0.3052711825249972 1104 6 0.2681529502981377 1104 7 0.3492167400856303 1104 8 0.3173324741649921 1104 9 0.33296269657072486 1104 10-11 0.23707182746612432 1104 12-13 0.4060644550614043 1104 14-15 0.44505513126830465 1104 16-17 0.47642470212750254 1104 18-19 0.46716594821390345 1104 20-21 0.4712512442022856 1104 22-23 0.48026233634496407 1104 24-25 0.4473040120719176 1104 26-27 0.3139435816706282 1104 28-29 0.3409564446653164 1104 30-31 0.41139954514188304 1104 32-33 0.44191565045348824 1104 34-35 0.4505417670940801 1104 36-37 0.344016517244782 1104 38-39 0.4392143774347588 1104 40-41 0.6278913141251934 1104 42-43 0.5463984018271262 1104 44-45 0.5774585956089737 1104 46-47 0.49638827181146894 1104 48-49 0.6141307169550245 1104 50-51 0.4286690174103498 1104 52-53 0.45199884267187684 1104 54-55 0.49025654168618615 1104 56-57 0.7186618247134007 1104 58-59 0.6607283927838026 1104 60-61 0.6303717262062136 1104 62-63 0.5520724764178837 1104 64-65 0.6109116717646828 1104 66-67 0.6818959606277417 1104 68-69 0.8516874040080538 1104 70-71 0.6869603931991008 1104 72-73 0.8694239196172724 1104 74-75 0.854710334742137 1104 76-77 0.8843172347542634 1104 78-79 0.7831243573107507 1104 80-81 1.067520448521602 1104 82-83 1.1653943719622752 1104 84-85 1.0646580477279386 1104 86-87 1.1313922626831534 1104 88-89 1.205771042706555 1104 90-91 1.3619468253021658 1104 92-93 1.5088366652869496 1104 94-95 1.536515954532053 1104 96-97 1.7187891935323734 1104 98-99 1.92926133656864 1104 100-101 1.8830904979393566 1105 1 0.16525163722426583 1105 2 0.2253307326094287 1105 3 0.23988340212311243 1105 4 0.16464418028508732 1105 5 0.18937874214724815 1105 6 0.21663897360950557 1105 7 0.15796657479027232 1105 8 0.2559024932791445 1105 9 0.21943634119171662 1105 10-11 0.16627872315918069 1105 12-13 0.35824729834765634 1105 14-15 0.3235925592896933 1105 16-17 0.39132710917140656 1105 18-19 0.31624409384898655 1105 20-21 0.4147405176068233 1105 22-23 0.3824665177594113 1105 24-25 0.39567668999412575 1105 26-27 0.3811211183318619 1105 28-29 0.2825078983199276 1105 30-31 0.2863532839082339 1105 32-33 0.25144172933844544 1105 34-35 0.4226928264441341 1105 36-37 0.3074798027841439 1105 38-39 0.3734903665879443 1105 40-41 0.4938250783427378 1105 42-43 0.34514003187121745 1105 44-45 0.3186195872780857 1105 46-47 0.4012375009769613 1105 48-49 0.4360623198820832 1105 50-51 0.3581081852834558 1105 52-53 0.08164729164327156 1105 54-55 0.2637070157618595 1105 56-57 0.38133027571127087 1105 58-59 0.4137871082714142 1105 60-61 0.42691986536686954 1105 62-63 0.5028717760807595 1105 64-65 0.5289250662701761 1105 66-67 0.5776967715624721 1105 68-69 0.6070294494801658 1105 70-71 0.647462101108033 1105 72-73 0.5494940005561837 1105 74-75 0.5938912168096948 1105 76-77 0.5919713544298197 1105 78-79 0.5550690335980377 1105 80-81 0.7643240012126107 1105 82-83 0.7079949574083848 1105 84-85 0.767171374397936 1105 86-87 0.8406594074612777 1105 88-89 0.7289590835658473 1105 90-91 0.7891243447898475 1105 92-93 0.853564644939734 1105 94-95 0.950406625176111 1105 96-97 1.0806618439769125 1105 98-99 1.3336005114828993 1105 100-101 1.3238781582329224 1106 1 0.19976131561620747 1106 2 0.2864285796150696 1106 3 0.20065321947980408 1106 4 0.07061904798664642 1106 5 0.1328420832922106 1106 6 0.12436656314466177 1106 7 0.16022147272028775 1106 8 0.18175736732398207 1106 9 0.22342658546661198 1106 10-11 0.015349966578511953 1106 12-13 -0.13277324486396225 1106 14-15 0.1505780881995662 1106 16-17 0.12007309323097815 1106 18-19 0.11709321685178509 1106 20-21 0.3313715767578813 1106 22-23 0.02655067777443776 1106 24-25 0.17654167608395 1106 26-27 0.252196705133926 1106 28-29 0.42166684145950484 1106 30-31 0.452991440089896 1106 32-33 0.3223165925370637 1106 34-35 0.20456546851689694 1106 36-37 0.1806220938426648 1106 38-39 0.28343149549547775 1106 40-41 0.060123458066186686 1106 42-43 0.3430908254660707 1106 44-45 0.17833405171168693 1106 46-47 0.2559839216262816 1106 48-49 0.49492239972123286 1106 50-51 0.49586656614241775 1106 52-53 0.43523432668987283 1106 54-55 0.49704514570897373 1106 56-57 0.6812671855564716 1106 58-59 0.3823067606833277 1106 60-61 0.23976297008771752 1106 62-63 0.4446445555911964 1106 64-65 0.414246228930196 1106 66-67 0.710666247478585 1106 68-69 0.7181315057591249 1106 70-71 0.37263081465621894 1106 72-73 0.4413077225914357 1106 74-75 0.43635647072676775 1106 76-77 0.5977715862335238 1106 78-79 0.6745980795417665 1106 80-81 0.5002835050665411 1106 82-83 0.47511385175954146 1106 84-85 0.6496150582364812 1106 86-87 0.6119292081766581 1106 88-89 0.9803230594300736 1106 90-91 1.0967284949375617 1106 92-93 1.1419057409561937 1106 94-95 1.1147426514654732 1106 96-97 1.0046902115424032 1106 98-99 1.2908172861223228 1106 100-101 1.210646625685449 1107 1 0.4166798282109845 1107 2 0.4790526197973719 1107 3 0.5726927074853805 1107 4 0.3852900232828702 1107 5 0.4088390792432719 1107 6 0.4852033087812302 1107 7 0.4287086327597933 1107 8 0.3635129257310794 1107 9 0.5108130743497981 1107 10-11 0.5665104552004792 1107 12-13 0.5660727111270702 1107 14-15 0.7855844239702066 1107 16-17 0.7485477421284656 1107 18-19 0.7285950465490174 1107 20-21 0.8090597537286683 1107 22-23 0.6276471785500917 1107 24-25 0.7507153048421529 1107 26-27 0.6664425364718198 1107 28-29 0.7012878581158191 1107 30-31 0.6789597646738343 1107 32-33 0.8074289636734449 1107 34-35 0.880851125844508 1107 36-37 0.8784404920956916 1107 38-39 0.9636876072800078 1107 40-41 0.9534802937096458 1107 42-43 0.940285471109739 1107 44-45 0.8849950318427986 1107 46-47 0.8231304875927705 1107 48-49 0.9030013092812581 1107 50-51 0.8391261310938134 1107 52-53 0.7344809084671411 1107 54-55 0.6366184206722565 1107 56-57 0.7839265765892307 1107 58-59 0.9958608415841255 1107 60-61 1.216855521604522 1107 62-63 1.102082727406291 1107 64-65 1.1803809620143824 1107 66-67 1.2736209507284997 1107 68-69 1.431832294694324 1107 70-71 1.390368394598056 1107 72-73 1.444621829018633 1107 74-75 1.460672138755296 1107 76-77 1.3629582451399216 1107 78-79 1.228753165000203 1107 80-81 1.5372115671456363 1107 82-83 1.7133308077941045 1107 84-85 1.6491287047760963 1107 86-87 1.6467132191204712 1107 88-89 1.6809965644517177 1107 90-91 1.9010322361356522 1107 92-93 2.047445329784157 1107 94-95 2.067675064947256 1107 96-97 2.242487694878065 1107 98-99 2.413545918077059 1107 100-101 2.4282961006070245 1108 1 0.19468553722104787 1108 2 0.15033234244876326 1108 3 0.16707036886383264 1108 4 0.3192430888140194 1108 5 0.27848334950743237 1108 6 0.17915185232421038 1108 7 0.24721204309253153 1108 8 0.1994212801042181 1108 9 0.25025470301920194 1108 10-11 0.4053710554944132 1108 12-13 0.2956366218931734 1108 14-15 0.34388156569685435 1108 16-17 0.4025497016566746 1108 18-19 0.42434178694921343 1108 20-21 0.39251869784443016 1108 22-23 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2474.0 8 3061.0 9 3824.0 10 4813.0 11 6062.0 12 7968.0 13 9965.0 14 11405.0 15 13731.0 16 16873.0 17 21107.0 18 19014.0 19 15323.0 20 15874.0 21 17264.0 22 19325.0 23 21322.0 24 23989.0 25 27490.0 26 31730.0 27 37938.0 28 45136.0 29 54155.0 30 66981.0 31 82437.0 32 103598.0 33 133999.0 34 178227.0 35 245122.0 36 338788.0 37 406027.0 38 239836.0 39 20296.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.444199292363834 18.01697616500264 14.328611319515042 24.210213223118483 2 15.914785799704031 19.271082238980483 39.110939534925 25.703192426390487 3 19.129903505019975 22.337159175009628 29.67886179583245 28.854075524137947 4 13.471240028736231 15.559364767765013 34.96497383520863 36.00442136829013 5 14.825119466161867 36.06140842495475 33.5366110703609 15.576861038522486 6 34.56077687984303 34.08950524903832 16.292793242209587 15.056924628909066 7 29.861978150604763 31.886239492072498 19.62206930798425 18.629713049338488 8 27.1328102692704 34.27309583812917 19.074827482854694 19.519266409745732 9 26.570783069088073 16.20932392973463 19.595949742036613 37.62394325914069 10-11 25.840708710176123 25.576651780300203 26.44311768092833 22.13952182859535 12-13 26.0682431433286 22.727628454440833 27.38245650751662 23.82167189471395 14-15 23.179163851918723 24.45934114612028 25.05664045135883 27.304854550602165 16-17 23.225517995970947 27.659805569742367 25.8051459113608 23.30953052292589 18-19 23.204463491607456 26.241324363077233 27.784494828979 22.769717316336312 20-21 23.665393026409525 25.50372627329669 27.902119881777104 22.92876081851668 22-23 23.494690350234716 25.07543728353069 28.059698977972225 23.370173388262376 24-25 23.48339397341147 25.33930623139704 27.940301167601373 23.236998627590115 26-27 23.12424629353763 25.792854862736753 27.812100978931685 23.270797864793927 28-29 23.054230419699724 25.95213015850564 27.946015897787333 23.047623524007307 30-31 23.25716530607992 25.504228527138796 27.82341149224685 23.415194674534437 32-33 23.15934825491304 25.537731379194824 27.79155491297568 23.51136545291645 34-35 23.663865576026733 25.58943064196883 27.481430507175947 23.265273274828495 36-37 23.643800994685215 25.517929448148536 27.467600247108585 23.37066931005766 38-39 23.25128487229514 25.627985559163523 27.578992301958788 23.541737266582548 40-41 23.454457287029108 25.55109936188962 27.571235565389912 23.423207785691364 42-43 23.467777294549137 25.58340991842303 27.301043906863665 23.647768880164175 44-45 23.343207976205097 25.717533490955212 27.317877344876706 23.621381187962985 46-47 23.491476243096372 26.049855748395945 26.86387148473305 23.594796523774637 48-49 23.749239264062112 25.94399431540417 26.65997847214293 23.646787948390788 50-51 23.578810381312483 26.231913457285668 26.61620780360949 23.57306835779236 52-53 23.76814711497737 26.197859266864317 26.352238633868996 23.681754984289316 54-55 23.63118128640452 26.0384791701211 26.453254582679765 23.877084960794623 56-57 23.585994135781263 26.40979497397005 26.509548747703406 23.494662142545284 58-59 23.704054535050044 26.044595408633892 26.462500060948123 23.78884999536794 60-61 23.50249112985808 26.066860183605456 26.921686801720778 23.508961884815687 62-63 23.34451700521671 26.211589694060567 26.885956465457344 23.557936835265377 64-65 23.382802653778214 26.23590011344399 26.655614059076417 23.725683173701384 66-67 23.328376599488518 26.225162774411732 26.597538327889055 23.848922298210702 68-69 23.190863832435333 26.09944808193847 26.963515432607664 23.74617265301853 70-71 23.183999730509832 26.190455083790166 26.785571815583626 23.839973370116375 72-73 23.341252498747856 26.273153586007897 26.404418185122314 23.981175730121933 74-75 23.31248812758658 26.600478908498243 26.256447676376133 23.830585287539048 76-77 23.503856746502642 26.33935557026256 26.413516100104744 23.743271583130053 78-79 23.523174322138694 26.375454849918643 26.356481759567945 23.744889068374718 80-81 23.480342844148996 26.41253221117544 26.403977286453234 23.703147658222335 82-83 23.462174319889368 26.421948783719134 26.40205061889275 23.713826277498743 84-85 23.49530353625218 26.27737388017204 26.211630035209627 24.015692548366154 86-87 23.559084515094543 26.23997397599161 26.36240470346724 23.838536805446605 88-89 23.518141003892115 26.323065239181226 26.168315842123256 23.9904779148034 90-91 23.410535292725328 26.356537950439566 26.284554495996144 23.948372260838962 92-93 23.366567066669653 26.011045604949757 26.47089132283319 24.1514960055474 94-95 23.364638209944342 26.090573611003997 26.446482090667573 24.098306088384085 96-97 23.46792684824144 26.06566619844979 26.27126645056884 24.195140502739935 98-99 23.249563025217533 26.16546926193264 26.2299903672716 24.354977345578234 100-101 23.70200509061932 25.529216547216315 26.556049331939313 24.212729030225052 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2692.0 1 1583.0 2 467.5 3 460.5 4 410.0 5 403.5 6 616.5 7 1137.0 8 1800.5 9 2162.0 10 2057.0 11 1970.0 12 2105.5 13 2263.5 14 2464.5 15 2612.5 16 2714.0 17 2824.0 18 3080.0 19 3264.5 20 3486.0 21 4071.5 22 4778.0 23 5585.0 24 6566.5 25 8182.5 26 10080.0 27 12243.0 28 14537.0 29 16882.0 30 19092.5 31 21571.0 32 24593.0 33 25906.0 34 27065.0 35 29055.5 36 31937.0 37 36554.5 38 42258.5 39 46398.0 40 49960.0 41 56185.5 42 61451.5 43 65994.0 44 72282.5 45 78718.0 46 83235.0 47 87012.0 48 90968.5 49 92740.0 50 93410.5 51 92430.0 52 92388.0 53 95049.5 54 97166.5 55 92746.5 56 83639.0 57 76413.5 58 70498.0 59 62851.5 60 52370.5 61 42787.5 62 34531.5 63 26869.5 64 20947.0 65 16320.0 66 11903.5 67 9122.0 68 6626.0 69 4259.5 70 3192.0 71 2438.0 72 1900.5 73 1473.5 74 1115.5 75 843.5 76 567.0 77 369.0 78 238.5 79 159.0 80 112.0 81 74.5 82 46.0 83 29.5 84 18.5 85 15.0 86 12.5 87 8.5 88 7.0 89 9.5 90 12.0 91 10.0 92 8.0 93 6.0 94 3.5 95 1.5 96 0.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3347145192654421 2 0.28847834310782317 3 0.28046997192645573 4 0.28909777513290136 5 0.29060211005094827 6 0.21945591745625814 7 0.20773095412442177 8 0.20826189586020302 9 0.21989836890274253 10-11 0.2185488919909651 12-13 0.2137704163689337 14-15 0.21682333134967602 16-17 0.19994380866629646 18-19 0.20711152209934364 20-21 0.19450165587453846 22-23 0.20436832313114037 24-25 0.20412497483557399 26-27 0.20419134255254662 28-29 0.2174870085194026 30-31 0.21494291270211735 32-33 0.22076114922338708 34-35 0.21339433263942198 36-37 0.19673603567928466 38-39 0.18456862090096388 40-41 0.18136084791395204 42-43 0.19795277715711673 44-45 0.20025352467883556 46-47 0.20031989239580822 48-49 0.21757549880869947 50-51 0.21381466151358217 52-53 0.23449926663672743 54-55 0.20251002705590598 56-57 0.1805201901656317 58-59 0.1822015056622724 60-61 0.1694367814311977 62-63 0.17779911376975271 64-65 0.17501166965690101 66-67 0.17454709563809243 68-69 0.1771133140277019 70-71 0.17799821692067067 72-73 0.17720180431699875 74-75 0.19381585613248767 76-77 0.1866260201271163 78-79 0.19063020571780004 80-81 0.18248909910248723 82-83 0.1612956748158849 84-85 0.16149477796680287 86-87 0.16622900844418587 88-89 0.1728436575691275 90-91 0.17967953241731136 92-93 0.17448072792111974 94-95 0.1741931344809049 96-97 0.17954679698336604 98-99 0.18954619967391328 100-101 0.16937041371422504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260135.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.924601892146505 #Duplication Level Percentage of deduplicated Percentage of total 1 65.30123408806385 26.07125774031816 2 15.21048266147924 12.145449296939114 3 6.826241894923188 8.176049702229001 4 3.7097078950216593 5.924344433795703 5 2.1992227036859737 4.390154545841629 6 1.4487319911543732 3.470402879715304 7 0.997444010012029 2.7875788506605517 8 0.7320805619798926 2.3382419992020878 9 0.5538281946980885 1.9900233170970651 >10 2.7433166837719347 19.357761622231404 >50 0.18425327464878197 5.058348216755165 >100 0.08911406036826283 6.318265851369049 >500 0.003562650414605553 0.9328550946784581 >1k 5.566641272821114E-4 0.4784066033270194 >5k 2.2266565091284458E-4 0.5608598458404098 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6989 0.3092293159479412 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5629 0.2490559192260639 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3598 0.15919403044508404 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3133 0.13862003818355983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.212257232421957E-5 0.0 14-15 0.0 0.0 0.0 4.424514464843914E-5 0.0 16-17 0.0 0.0 0.0 1.1061286162109785E-4 0.0 18-19 0.0 0.0 0.0 1.54858006269537E-4 0.0 20-21 0.0 0.0 0.0 1.9910315091797615E-4 0.0 22-23 0.0 0.0 0.0 2.875934402148544E-4 0.0 24-25 0.0 0.0 0.0 4.2032887416017185E-4 0.0 26-27 0.0 0.0 0.0 6.415545974023675E-4 0.0 28-29 0.0 0.0 0.0 0.001703438068964907 0.0 30-31 0.0 0.0 0.0 0.004557249898789232 0.0 32-33 0.0 0.0 0.0 0.008915396646660488 0.0 34-35 0.0 0.0 0.0 0.013008072526641108 0.0 36-37 0.0 0.0 0.0 0.017344096702188144 0.0 38-39 0.0 0.0 0.0 0.022410165764434425 0.0 40-41 0.0 0.0 0.0 0.029223918040294054 0.0 42-43 0.0 0.0 0.0 0.037674740668145926 0.0 44-45 0.0 0.0 0.0 0.04663438245945485 0.0 46-47 0.0 0.0 0.0 0.0577620363385373 0.0 48-49 0.0 0.0 0.0 0.06835874848183848 0.0 50-51 0.0 0.0 0.0 0.08446398113387033 0.0 52-53 0.0 0.0 0.0 0.12244843781455532 0.0 54-55 0.0 0.0 0.0 0.17717968174467452 0.0 56-57 0.0 0.0 0.0 0.22111511038057463 0.0 58-59 0.0 0.0 0.0 0.2813769973917487 0.0 60-61 0.0 0.0 0.0 0.356062801558314 0.0 62-63 0.0 0.0 0.0 0.4074092919228276 0.0 64-65 0.0 0.0 0.0 0.45366759065277074 0.0 66-67 0.0 0.0 0.0 0.574191364675119 0.0 68-69 0.0 0.0 0.0 0.6942726872509828 0.0 70-71 0.0 0.0 0.0 0.7568353217838757 0.0 72-73 0.0 0.0 0.0 0.8715629818572784 0.0 74-75 0.0 0.0 0.0 1.0406458021312885 0.0 76-77 0.0 0.0 0.0 1.2141973820147913 0.0 78-79 0.0 0.0 0.0 1.3901381997093094 0.0 80-81 0.0 0.0 0.0 1.6063642216062315 0.0 82-83 0.0 0.0 0.0 1.8443809772425097 0.0 84-85 0.0 0.0 0.0 2.0948306185249996 0.0 86-87 0.0 0.0 0.0 2.4378189798397 0.0 88-89 4.424514464843914E-5 0.0 0.0 2.8247648923626243 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4040 0.0 46.260754 1 GTATCAA 6525 0.0 39.647873 1 ATCAACG 8310 0.0 30.371319 3 TATCAAC 8455 0.0 30.021107 2 TCAACGC 8505 0.0 29.679585 4 CAACGCA 8665 0.0 29.132195 5 AACGCAG 8755 0.0 28.761187 6 GTACATG 6040 0.0 25.982353 1 TACATGG 6330 0.0 24.630272 2 ACGCAGA 10225 0.0 24.347538 7 CGCAGAG 10310 0.0 24.192888 8 ACATGGG 6395 0.0 24.155329 3 GTCGTAA 165 2.4070232E-6 23.05725 1 GAGTACT 6800 0.0 21.620304 12-13 GCAGAGT 11265 0.0 21.382765 9 CATGGGG 4555 0.0 21.185818 4 AGTACTT 7160 0.0 20.699108 12-13 AGAGTAC 9810 0.0 20.530848 10-11 CCGATCG 280 1.5734258E-9 20.361525 9 CAGAGTA 10435 0.0 19.756376 10-11 >>END_MODULE