FastQCFastQC Report
Thu 26 May 2016
SRR937574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260135
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94090.41630256599716386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.3405106332143876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53630.23728671074957913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.047.819191
GTATCAA77500.041.689671
ATCAACG100950.031.5769523
TATCAAC102450.031.0682542
TCAACGC103100.030.8723814
CAACGCA105100.030.2848955
AACGCAG106900.029.858556
CTACGCG404.4932088E-429.69031590-91
ACGCAGA121550.026.1828047
CGCAGAG123100.025.6216098
TACGCGC500.001654652823.74936192-93
GCAGAGT134100.023.2011159
GTACATG64850.023.0792561
GAGTACT86300.022.67660112-13
TACATGG65650.022.5050322
AGAGTAC116400.022.21985810-11
AGTACTT90050.021.70589312-13
ACATGGG67250.021.263183
GTACTTT92900.021.03883214-15
CAGAGTA123800.020.89169110-11
Produced by FastQC (version 0.11.5)