##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937574_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260135 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16319644622998 33.0 31.0 34.0 31.0 34.0 2 32.358644948199995 34.0 31.0 34.0 31.0 34.0 3 32.46390326241574 34.0 31.0 34.0 31.0 34.0 4 36.11084249392182 37.0 35.0 37.0 35.0 37.0 5 36.008881327885284 37.0 35.0 37.0 35.0 37.0 6 36.03752563453068 37.0 35.0 37.0 35.0 37.0 7 35.97980518862811 37.0 35.0 37.0 35.0 37.0 8 35.95597386881757 37.0 35.0 37.0 35.0 37.0 9 37.62245972032644 39.0 37.0 39.0 35.0 39.0 10-11 37.55618823654339 39.0 37.0 39.0 35.0 39.0 12-13 37.45565441887321 39.0 37.0 39.0 35.0 39.0 14-15 38.69099721919266 40.0 38.0 41.0 34.5 41.0 16-17 38.637853933503976 40.0 38.0 41.0 34.0 41.0 18-19 38.59709552747955 40.0 38.0 41.0 34.0 41.0 20-21 38.4696186289757 40.0 38.0 41.0 34.0 41.0 22-23 38.306653584852235 40.0 38.0 41.0 34.0 41.0 24-25 38.37365046778179 40.0 38.0 41.0 34.0 41.0 26-27 38.24625962608428 40.0 38.0 41.0 34.0 41.0 28-29 38.09783309404084 40.0 38.0 41.0 33.0 41.0 30-31 38.160327148599535 40.0 38.0 41.0 34.0 41.0 32-33 38.10317658016004 40.0 37.0 41.0 33.5 41.0 34-35 37.97575985505291 40.0 37.0 41.0 33.0 41.0 36-37 37.812611636030596 40.0 37.0 41.0 33.0 41.0 38-39 37.660783316040856 40.0 37.0 41.0 33.0 41.0 40-41 37.48135730830238 40.0 37.0 41.0 33.0 41.0 42-43 37.26512398595659 40.0 36.0 41.0 32.0 41.0 44-45 36.962424147230145 39.0 36.0 41.0 31.0 41.0 46-47 36.62608737973616 39.0 36.0 41.0 30.5 41.0 48-49 36.304075199047844 39.0 35.0 41.0 30.0 41.0 50-51 36.03034774471436 39.0 35.0 40.0 29.5 41.0 52-53 35.75811599749572 39.0 35.0 40.0 29.0 41.0 54-55 35.50317834996582 38.0 35.0 40.0 28.0 41.0 56-57 35.19236837622532 38.0 34.0 40.0 28.0 41.0 58-59 34.886071186013226 38.0 34.0 40.0 27.0 41.0 60-61 34.50645492415276 37.0 34.0 40.0 26.0 41.0 62-63 34.12618560395728 36.5 33.5 39.5 26.0 41.0 64-65 33.69567459465917 36.0 33.0 39.0 26.0 40.5 66-67 33.23146758932542 35.5 32.5 39.0 25.0 40.0 68-69 32.773830324294785 35.0 32.0 38.0 23.5 40.0 70-71 32.34731487278415 35.0 31.5 37.5 23.0 39.5 72-73 31.859793109703624 35.0 31.0 37.0 22.0 39.0 74-75 31.36721257800972 34.5 31.0 36.0 20.0 39.0 76-77 30.1453231775978 33.5 29.5 35.0 20.0 37.0 78-79 30.769684996692675 34.0 30.5 35.0 20.0 37.0 80-81 30.602900048005985 34.0 30.5 35.0 18.5 37.0 82-83 30.27639610023295 34.0 30.0 35.0 18.0 36.0 84-85 29.925137657706287 34.0 30.0 35.0 16.0 36.0 86-87 29.51876082623383 34.0 29.5 35.0 9.0 35.5 88-89 29.159415256168327 34.0 29.0 35.0 6.0 35.0 90-91 28.647314651558425 33.5 29.0 35.0 2.0 35.0 92-93 28.16956442867351 33.0 28.0 35.0 2.0 35.0 94-95 27.69157882161906 33.0 27.0 35.0 2.0 35.0 96-97 27.118046045922036 33.0 26.5 35.0 2.0 35.0 98-99 26.201257004559462 32.5 24.5 34.0 2.0 35.0 100-101 23.530841962980087 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.323888569549176 1101 2 0.3425963584564684 1101 3 0.26923307030740773 1101 4 0.20168390413520854 1101 5 0.20916779030333288 1101 6 0.2142994274043275 1101 7 0.2602537554361106 1101 8 0.18905706596532923 1101 9 0.28365482579921064 1101 10-11 0.28915744212397954 1101 12-13 0.308575917210824 1101 14-15 0.30531008221174716 1101 16-17 0.3490865929108651 1101 18-19 0.2895803910507837 1101 20-21 0.30859372925050366 1101 22-23 0.33053183764452854 1101 24-25 0.31974153042812503 1101 26-27 0.2712279300233291 1101 28-29 0.288740007931203 1101 30-31 0.36574454532209444 1101 32-33 0.3592593403519402 1101 34-35 0.3302722830659448 1101 36-37 0.3600054129688459 1101 38-39 0.25151217248119906 1101 40-41 0.27648066547448025 1101 42-43 0.3952292739998242 1101 44-45 0.29669342876049853 1101 46-47 0.23381341907697362 1101 48-49 0.3445000961168958 1101 50-51 0.27171462335857655 1101 52-53 0.25744303488470877 1101 54-55 0.29991187250817575 1101 56-57 0.2379765778147842 1101 58-59 0.1681454104335387 1101 60-61 0.14082898142423517 1101 62-63 0.2358825836269105 1101 64-65 0.22685752620925115 1101 66-67 0.09719711085323013 1101 68-69 0.04496917982296367 1101 70-71 -0.032087174882271086 1101 72-73 0.03375525546157476 1101 74-75 0.025437511216232167 1101 76-77 0.04888014132562901 1101 78-79 0.07375444416513588 1101 80-81 0.1553318120866578 1101 82-83 0.09071219108782458 1101 84-85 0.15451609378629882 1101 86-87 -0.1149432330041229 1101 88-89 0.01994453953224351 1101 90-91 0.2026779999349344 1101 92-93 0.2412673403823291 1101 94-95 0.2562067020336194 1101 96-97 0.5302937944012882 1101 98-99 0.373192806128813 1101 100-101 0.7026346528962151 1102 1 0.2533264606420431 1102 2 0.23194802188587715 1102 3 0.24550958972879044 1102 4 0.1570879524261528 1102 5 0.13021987427913473 1102 6 0.18033139702855294 1102 7 0.17836896973704341 1102 8 0.2178003703205249 1102 9 0.19430638681499346 1102 10-11 0.2147432460165959 1102 12-13 0.2562153327538894 1102 14-15 0.2985747411113735 1102 16-17 0.29515549742998104 1102 18-19 0.32262308064300527 1102 20-21 0.3038758222753728 1102 22-23 0.32880488478835446 1102 24-25 0.2092515103672028 1102 26-27 0.3196399751258241 1102 28-29 0.2536312363342219 1102 30-31 0.20456427840177582 1102 32-33 0.23478450780281435 1102 34-35 0.2412730027546104 1102 36-37 0.11809628645792714 1102 38-39 0.15287686266918854 1102 40-41 0.12897082763595336 1102 42-43 0.23510576275148054 1102 44-45 0.11205054262708813 1102 46-47 0.12647707513665551 1102 48-49 0.10192798992991925 1102 50-51 -0.008387681538579272 1102 52-53 0.23279430725640538 1102 54-55 0.32474525586415126 1102 56-57 0.17587488818675467 1102 58-59 0.27401069498802144 1102 60-61 0.2943989015498474 1102 62-63 0.33021175936310243 1102 64-65 0.29219847098294593 1102 66-67 0.216635153794968 1102 68-69 0.2573847560678786 1102 70-71 0.056239564898056926 1102 72-73 0.16635381522188553 1102 74-75 0.10018914926251199 1102 76-77 0.020445971280011577 1102 78-79 0.1517347454295539 1102 80-81 0.06048391310327972 1102 82-83 -0.0013232230687307833 1102 84-85 0.07733093695734894 1102 86-87 0.14177771994690502 1102 88-89 0.1240483771656784 1102 90-91 0.3407040320807795 1102 92-93 0.3656444085030479 1102 94-95 0.19860386332205948 1102 96-97 0.4136201508100896 1102 98-99 0.21000213512363786 1102 100-101 0.6060845467988436 1103 1 0.2598588999828948 1103 2 0.22932501218310364 1103 3 0.23079674112570814 1103 4 0.13314821730104853 1103 5 0.09483196513597392 1103 6 0.10176634875099921 1103 7 0.18409087544903002 1103 8 0.18297923486088763 1103 9 0.22515810696766891 1103 10-11 0.2053158619407185 1103 12-13 0.2550068941881207 1103 14-15 0.2660137929451736 1103 16-17 0.31696864970596295 1103 18-19 0.3097039853141368 1103 20-21 0.2603429499470522 1103 22-23 0.331618485320881 1103 24-25 0.22110676894723724 1103 26-27 0.23199673207516014 1103 28-29 0.2612598683301428 1103 30-31 0.28646162147290255 1103 32-33 0.21792835307323344 1103 34-35 0.2649511640084512 1103 36-37 0.1991590107452481 1103 38-39 0.048472351310635986 1103 40-41 0.027271760831183656 1103 42-43 0.15105956942385035 1103 44-45 0.07336851138872902 1103 46-47 0.17326707839769284 1103 48-49 0.003981844699886494 1103 50-51 -0.039890064423829585 1103 52-53 -0.1117772805497097 1103 54-55 -0.10121988628188916 1103 56-57 -0.10803519220494451 1103 58-59 -0.03368097649264712 1103 60-61 0.015002651853869509 1103 62-63 0.19779803057031842 1103 64-65 0.26289038204274107 1103 66-67 0.16107688171669565 1103 68-69 0.07201367926695212 1103 70-71 0.10232073750518822 1103 72-73 0.01712065019986042 1103 74-75 0.1494812820147935 1103 76-77 8.551062612198734E-4 1103 78-79 0.05232449544200435 1103 80-81 0.2608181423437834 1103 82-83 0.3689279569220858 1103 84-85 0.16138549192366725 1103 86-87 0.37508289031165987 1103 88-89 0.22836109227337786 1103 90-91 0.20851952125300244 1103 92-93 0.21036886887742412 1103 94-95 0.3683563079198393 1103 96-97 0.2905323757803373 1103 98-99 0.4996270320119116 1103 100-101 0.5924109953527719 1104 1 0.1601032981686572 1104 2 0.1450459810796616 1104 3 0.09321214212239681 1104 4 0.04492676049001432 1104 5 0.10978342866853552 1104 6 0.1256300639791803 1104 7 0.04853462034701295 1104 8 0.13221120570181455 1104 9 0.20850095575175231 1104 10-11 0.28766930834240156 1104 12-13 0.22027658532388017 1104 14-15 0.16700281189772426 1104 16-17 0.1776565075179093 1104 18-19 0.1821930470191191 1104 20-21 0.09918009923815418 1104 22-23 0.21020456091254403 1104 24-25 7.813635372784233E-4 1104 26-27 0.08425948581465548 1104 28-29 -0.06465770805579751 1104 30-31 0.12995111683704152 1104 32-33 0.16055823705886496 1104 34-35 0.12465456568303779 1104 36-37 0.14300312120526115 1104 38-39 0.1516987601181654 1104 40-41 0.17874716054721773 1104 42-43 0.27656699090029235 1104 44-45 0.27795498421371434 1104 46-47 0.2673016192922191 1104 48-49 0.08010618563190519 1104 50-51 0.1865140460975141 1104 52-53 0.21984879942839086 1104 54-55 0.06160187366878489 1104 56-57 0.18260204519832968 1104 58-59 0.1320491947202811 1104 60-61 0.13788108657052334 1104 62-63 0.11652096761228137 1104 64-65 0.11954877557136712 1104 66-67 0.060555091206666134 1104 68-69 0.15671227848269353 1104 70-71 0.1834174925767158 1104 72-73 0.20459762044454877 1104 74-75 0.08222682378008273 1104 76-77 0.07353068741625535 1104 78-79 0.17997672763638306 1104 80-81 0.3009301767113648 1104 82-83 0.3547044349578812 1104 84-85 0.3492832764276592 1104 86-87 0.2577058112112347 1104 88-89 0.38904887339935 1104 90-91 0.18435318401648004 1104 92-93 0.17597837471686617 1104 94-95 0.2834987770316886 1104 96-97 0.6108093156573915 1104 98-99 0.5146173178166862 1104 100-101 0.8699366573539926 1105 1 0.09902580426955865 1105 2 0.10295255184925622 1105 3 0.08425034295325418 1105 4 0.0599506059555992 1105 5 -0.03454024282904555 1105 6 0.06230627054943483 1105 7 0.06534601705416065 1105 8 0.037591848713638853 1105 9 0.18536003480329555 1105 10-11 0.07821947999197221 1105 12-13 0.1128177503083947 1105 14-15 0.14759176999599788 1105 16-17 0.09655315884860016 1105 18-19 0.19173917601647616 1105 20-21 0.17219760680738716 1105 22-23 0.12009551320272038 1105 24-25 0.23296359716272974 1105 26-27 0.08341315210866185 1105 28-29 0.13905497598019423 1105 30-31 0.22766890313290133 1105 32-33 0.29198221579114403 1105 34-35 0.18852007708542118 1105 36-37 0.18192234286590292 1105 38-39 0.15867497561866628 1105 40-41 0.3059443008087044 1105 42-43 0.3589084447438182 1105 44-45 0.24794689976797457 1105 46-47 0.3517223599528094 1105 48-49 0.3514652440751931 1105 50-51 0.5139484818006252 1105 52-53 0.5110919235707883 1105 54-55 0.30534158027595737 1105 56-57 0.29154639175357033 1105 58-59 0.5478284185104485 1105 60-61 0.5447809442018539 1105 62-63 0.5740791301117909 1105 64-65 0.5760135200032721 1105 66-67 0.466164571689788 1105 68-69 0.37670601872596876 1105 70-71 0.44953497077919735 1105 72-73 0.4220884240118359 1105 74-75 0.20076152120800117 1105 76-77 0.17089577205606687 1105 78-79 0.4909234879332125 1105 80-81 0.4131684089698062 1105 82-83 0.45753768328217603 1105 84-85 0.4572057971260719 1105 86-87 0.5972018707416034 1105 88-89 0.47163869962026084 1105 90-91 0.32298189371661223 1105 92-93 0.3972834223648647 1105 94-95 0.6845879661141225 1105 96-97 0.6638917735693255 1105 98-99 0.8233609271116933 1105 100-101 1.065879493496059 1106 1 0.03493252089932497 1106 2 0.1047632735536439 1106 3 0.17033120603048246 1106 4 0.0417905282700346 1106 5 -0.0027668368221185347 1106 6 0.046715562667131394 1106 7 0.04795217394845963 1106 8 0.054452435623389306 1106 9 0.036548028781098196 1106 10-11 -0.09593039363193157 1106 12-13 -0.17132104705326867 1106 14-15 0.0635896027096905 1106 16-17 0.12478123098184568 1106 18-19 0.12624704344199245 1106 20-21 0.0953483898301215 1106 22-23 0.050248128727702124 1106 24-25 0.09205401235370658 1106 26-27 0.1064455860279594 1106 28-29 0.12147159321236956 1106 30-31 0.10316728598882463 1106 32-33 0.20815404811719418 1106 34-35 -0.013100077111026565 1106 36-37 0.06176043757259464 1106 38-39 0.14849232741798346 1106 40-41 0.20490754700028901 1106 42-43 0.13955134272082148 1106 44-45 0.26637923423095344 1106 46-47 0.38980294600112586 1106 48-49 0.21048725857466621 1106 50-51 0.2502592886693833 1106 52-53 0.17435739950255424 1106 54-55 0.2557459397126607 1106 56-57 0.13369876451115204 1106 58-59 0.18667570411649592 1106 60-61 0.1717465769535167 1106 62-63 0.21529143998478872 1106 64-65 0.09210187656591984 1106 66-67 0.11370498560819442 1106 68-69 0.14951758815842453 1106 70-71 0.33481771158388796 1106 72-73 0.36316092238196873 1106 74-75 0.14759240337379964 1106 76-77 0.14726718756616108 1106 78-79 0.263477851187627 1106 80-81 0.32239995717969094 1106 82-83 0.19754628781996786 1106 84-85 0.41315930031826653 1106 86-87 0.36883019386500493 1106 88-89 0.22415802312151456 1106 90-91 0.46893550948239593 1106 92-93 0.46630706183962545 1106 94-95 0.7080387577511793 1106 96-97 0.790333816770179 1106 98-99 0.8158148257798032 1106 100-101 0.8388513579300501 1107 1 0.29197548040309584 1107 2 0.3448236753308649 1107 3 0.28090472502977804 1107 4 0.20127493631657245 1107 5 0.1542365384365212 1107 6 0.2216687604554579 1107 7 0.2956326861261971 1107 8 0.20725019965775004 1107 9 0.3160448792271211 1107 10-11 0.2784593731246332 1107 12-13 0.31783295571018044 1107 14-15 0.364529685567355 1107 16-17 0.3646815199296469 1107 18-19 0.33568149857718055 1107 20-21 0.4995507930692611 1107 22-23 0.4996753888105019 1107 24-25 0.48458078184393827 1107 26-27 0.5149040116863404 1107 28-29 0.5545855328243405 1107 30-31 0.5553055799365012 1107 32-33 0.5369663660926776 1107 34-35 0.5671914943663126 1107 36-37 0.4115389433847767 1107 38-39 0.5953985981123537 1107 40-41 0.6250655230824691 1107 42-43 0.670083341226821 1107 44-45 0.7150566277888615 1107 46-47 0.5660798672977947 1107 48-49 0.48515251105455803 1107 50-51 0.6792127293480092 1107 52-53 0.6091334764117065 1107 54-55 0.5120845746233371 1107 56-57 0.5166325127438967 1107 58-59 0.47343148020829773 1107 60-61 0.6153078070783309 1107 62-63 0.6283471661236817 1107 64-65 0.8289998351416656 1107 66-67 0.8096885309358726 1107 68-69 0.8181201210918303 1107 70-71 0.7510937392275636 1107 72-73 0.8361907898692671 1107 74-75 0.8164600247215645 1107 76-77 0.7613593220448998 1107 78-79 0.8412286697966884 1107 80-81 0.8190613612231274 1107 82-83 0.7182877734837092 1107 84-85 0.6873131369539145 1107 86-87 0.8985415783168165 1107 88-89 1.0025423901516781 1107 90-91 0.9666396519077729 1107 92-93 1.0902159172534454 1107 94-95 1.320554969533191 1107 96-97 1.4667638334287858 1107 98-99 1.7949001948470347 1107 100-101 2.01050434101251 1108 1 0.11139741958235305 1108 2 0.1250314983703973 1108 3 0.10335309899819833 1108 4 0.0644400454095404 1108 5 -0.035882446377137 1108 6 0.024462972310601572 1108 7 0.044532806712553 1108 8 -0.02879993788605617 1108 9 0.0993121098861991 1108 10-11 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74-75 -0.06776668480014791 2316 76-77 -0.07925473996335697 2316 78-79 -0.011553785773219971 2316 80-81 -0.009083172845592458 2316 82-83 -0.1531334981879695 2316 84-85 -0.1294654489685989 2316 86-87 -0.08009515994687533 2316 88-89 -0.0888529259318318 2316 90-91 0.08738127926359596 2316 92-93 -0.018439039042405625 2316 94-95 0.03026588487737314 2316 96-97 0.10365771762973708 2316 98-99 0.14985427375619764 2316 100-101 0.7870963214269295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 3.0 4 19.0 5 46.0 6 93.0 7 223.0 8 550.0 9 1090.0 10 1905.0 11 3005.0 12 4601.0 13 6521.0 14 8145.0 15 11179.0 16 14814.0 17 20303.0 18 18535.0 19 12450.0 20 11392.0 21 11877.0 22 12489.0 23 13813.0 24 15832.0 25 18899.0 26 23367.0 27 29033.0 28 36586.0 29 45864.0 30 57601.0 31 72711.0 32 93136.0 33 122675.0 34 168883.0 35 242561.0 36 365105.0 37 496980.0 38 300121.0 39 17722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74495902966963 18.59158183179595 14.943284105211172 23.720175033323244 2 16.35552743530807 19.719839744086084 38.504204394870214 25.42042842573563 3 19.45352821844713 22.51232780342767 30.02794080884549 28.006203169279708 4 14.013997449699708 16.089545450120923 34.77093754949945 35.12551955067992 5 14.847914332095899 36.2035497867685 33.515451981751845 15.433083899383762 6 34.68045050406281 34.399715061268466 15.980682569846492 14.939151864822234 7 29.99727892360412 32.18011313483486 19.360038227804978 18.462569713756036 8 27.42508743946711 34.30357036194741 18.83971532673933 19.43162687184615 9 26.67929128127302 16.027449687739892 19.65387023341526 37.639388797571826 10-11 26.000560143779083 25.69358719438759 26.265699290396054 22.040153371437267 12-13 26.29087052402056 22.74940001699067 27.401480666034235 23.558248792954537 14-15 23.252991524842543 24.42758950239698 25.199512418505975 27.119906554254502 16-17 23.25186327365401 27.638260546383293 26.046231751643155 23.063644428319545 18-19 23.154435465138146 25.933406632789634 28.31797658104494 22.594181321027285 20-21 23.533638477347594 25.17449178920728 28.624993639760454 22.666876093684667 22-23 23.510222249359437 24.819732943265375 28.67118943737033 22.998855370004854 24-25 23.487916316484707 24.972562473605002 28.625995488321603 22.913525721588694 26-27 23.14085257344515 25.511587427581343 28.484413673244184 22.863146325729318 28-29 23.031869059325718 25.695503421850507 28.49081293699525 22.78181458182852 30-31 23.250381061308286 25.227740820791677 28.444805288179687 23.07707282972035 32-33 23.257416039307387 25.14544485174558 28.471440865258046 23.125698243688984 34-35 23.73422384061129 25.11712795917058 28.218402883013628 22.930245317204502 36-37 23.78813654936541 25.211967426724506 28.042550555608404 22.95734546830167 38-39 23.316919111194096 25.270100195408812 28.240654810877324 23.17232588251977 40-41 23.54119536077936 25.172723053323253 28.119091853931717 23.16698973196568 42-43 23.496161955244457 25.254119871608648 27.882483014608088 23.367235158538808 44-45 23.425132442339162 25.371698011777617 27.851284956713858 23.351884589169362 46-47 23.731701867366333 25.727224258727908 27.332415984000956 23.208657889904806 48-49 23.926402626391784 25.620239498967983 27.116145716959384 23.33721215768085 50-51 23.738670477648462 25.940751326801276 26.9923920473777 23.328186148172563 52-53 24.042324905370695 25.89148435823524 26.69101624460486 23.37517449178921 54-55 23.76012937280295 25.84338988600238 26.76426408157035 23.632216659624312 56-57 23.688961057635936 26.182440429443375 26.850586358779456 23.278012154141233 58-59 23.987416680861983 25.776867311023455 26.775723574034295 23.459992434080267 60-61 23.678607693788205 25.820824862231678 27.28257382855449 23.217993615425627 62-63 23.473996022361497 25.880688542941023 27.319518524335933 23.32579691036155 64-65 23.583812471378923 25.8841396642236 27.08198846529079 23.45005939910669 66-67 23.558924577514176 25.884847586537973 26.965225528563558 23.591002307384294 68-69 23.410060381402335 25.66233934020697 27.39071107762242 23.536889200768275 70-71 23.379719963506588 25.885611207123116 27.101667423258974 23.63300140611132 72-73 23.550274651735407 25.928805137746195 26.67803029465054 23.842889915867858 74-75 23.458156260577354 26.31723326261484 26.49032026847954 23.73429020832826 76-77 23.675687514241407 26.03605536837401 26.72475316739929 23.563503949985286 78-79 23.657480637218573 26.050169569516868 26.692985153541716 23.599364639722847 80-81 23.58729093334932 26.178619015815208 26.675934551682655 23.558155499152818 82-83 23.599338874276604 26.20654227751219 26.615420795107443 23.578698053103768 84-85 23.51613295724794 26.086720867208673 26.464000884906806 23.933145290636578 86-87 23.546690795018883 25.877834731111193 26.696790235981478 23.878684237888447 88-89 23.612704550834355 26.122421890727765 26.524344784714188 23.740528773723693 90-91 23.558211081893436 26.06996237594627 26.486316061964082 23.885510480196206 92-93 23.37166005445369 25.62958348257179 26.788013108353642 24.210743354620874 94-95 23.35170244255321 25.745409013178417 26.8208536215757 24.08203492269267 96-97 23.53304116789484 25.629707959922747 26.67997708101507 24.15727379116734 98-99 23.234559439881828 25.736932681354695 26.522320195018395 24.506187683745082 100-101 23.254249124109784 25.55827076237077 26.895593709079208 24.291886404440234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 241.0 2 269.0 3 387.5 4 323.5 5 301.5 6 635.5 7 1422.5 8 2354.5 9 2781.5 10 2506.5 11 2179.5 12 2269.5 13 2504.0 14 2753.5 15 2990.0 16 3202.0 17 3332.5 18 3421.0 19 3635.0 20 3968.0 21 4377.5 22 5037.0 23 5879.5 24 6797.0 25 8451.5 26 10254.5 27 12324.0 28 14691.0 29 16929.5 30 19052.5 31 21632.5 32 24410.0 33 25560.0 34 26848.0 35 28775.0 36 31657.0 37 36475.0 38 42090.5 39 45914.0 40 49389.0 41 55774.5 42 61306.5 43 65636.0 44 71528.5 45 78204.5 46 82858.5 47 86995.5 48 91327.0 49 92697.0 50 93055.5 51 92359.5 52 92626.5 53 95072.0 54 97607.5 55 93418.0 56 83489.0 57 75927.5 58 70749.5 59 63513.0 60 52697.0 61 42713.5 62 34826.5 63 27449.0 64 20862.0 65 15897.5 66 11740.5 67 9297.5 68 6673.5 69 4157.0 70 3048.5 71 2194.0 72 1684.0 73 1279.5 74 997.5 75 818.5 76 563.0 77 342.5 78 218.5 79 147.0 80 83.5 81 49.0 82 32.0 83 21.0 84 15.0 85 6.0 86 3.0 87 3.0 88 1.5 89 0.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019954560236446053 2 0.0 3 0.0 4 3.982063018359523E-4 5 0.02738774453738383 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.424514464843914E-5 12-13 0.003141405270039179 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.079223143750263E-4 24-25 2.212257232421957E-5 26-27 5.751868804297088E-4 28-29 0.0012167414778320763 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.636771697265872E-5 40-41 6.19432025078148E-4 42-43 0.014401794583066941 44-45 2.212257232421957E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.849028929687828E-5 70-71 4.424514464843914E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.212257232421957E-5 82-83 0.0022343798047461767 84-85 4.424514464843914E-4 86-87 0.0 88-89 0.0 90-91 0.01227802763994186 92-93 0.0021901346600977376 94-95 0.0 96-97 0.0 98-99 0.0017919283582617854 100-101 0.019202392777422587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260135.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.57480735892357 #Duplication Level Percentage of deduplicated Percentage of total 1 62.92604535061499 23.644340319082467 2 16.07254568481724 12.078456157490121 3 7.184579009359567 8.098775166949551 4 3.9677363035417366 5.963477090263531 5 2.4570349976054615 4.616130835457923 6 1.5787368840112386 3.559244057228974 7 1.1046691813726366 2.905541217979163 8 0.7923098874376956 2.381671311123347 9 0.5961917343844577 2.016161061163068 >10 3.011740141623256 20.164642457826023 >50 0.20072567275405878 5.19183849805579 >100 0.101652692447358 6.739477976151987 >500 0.005204475320426443 1.3520110757404473 >1k 4.7313412003876577E-4 0.2896468172452489 >5k 3.5485059002907435E-4 0.9985859582424814 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9409 0.41630256599716386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7696 0.3405106332143876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5363 0.23728671074957913 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.212257232421957E-5 0.0 14-15 0.0 0.0 0.0 4.424514464843914E-5 0.0 16-17 0.0 0.0 0.0 1.1061286162109785E-4 0.0 18-19 0.0 0.0 0.0 1.54858006269537E-4 0.0 20-21 0.0 0.0 0.0 1.7698057859375657E-4 0.0 22-23 0.0 0.0 0.0 2.4334829556641528E-4 0.0 24-25 0.0 0.0 0.0 3.7608372951173274E-4 0.0 26-27 0.0 0.0 0.0 5.973094527539284E-4 0.0 28-29 0.0 0.0 0.0 0.0016370703519922483 0.0 30-31 0.0 0.0 0.0 0.004535127326465012 0.0 32-33 0.0 0.0 0.0 0.009048132080605804 0.0 34-35 0.0 0.0 0.0 0.013185053105234865 0.0 36-37 0.0 0.0 0.0 0.017675935287051435 0.0 38-39 0.0 0.0 0.0 0.022874739783243038 0.0 40-41 0.0 0.0 0.0 0.02986547263769642 0.0 42-43 0.0 0.0 0.0 0.03840478555484518 0.0 44-45 0.0 0.0 0.0 0.04718744676756034 0.0 46-47 0.0 0.0 0.0 0.05829297807431857 0.0 48-49 0.0 0.0 0.0 0.06904454822388928 0.0 50-51 0.0 0.0 0.0 0.0854594968884602 0.0 52-53 0.0 0.0 0.0 0.1241076307388718 0.0 54-55 0.0 0.0 0.0 0.17923708097082697 0.0 56-57 0.0 0.0 0.0 0.22332736761299657 0.0 58-59 0.0 0.0 0.0 0.28394321578135817 0.0 60-61 0.0 0.0 0.0 0.35909359396673207 0.0 62-63 0.0 0.0 0.0 0.4106613100544879 0.0 64-65 0.0 0.0 0.0 0.4569859765014037 0.0 66-67 0.0 0.0 0.0 0.5777530988193184 0.0 68-69 0.0 0.0 0.0 0.6984096082756118 0.0 70-71 0.0 0.0 0.0 0.7615474296889345 0.0 72-73 0.0 0.0 0.0 0.8773812183785481 0.0 74-75 0.0 0.0 0.0 1.0483223347277928 0.0 76-77 0.0 0.0 0.0 1.2240861718437173 0.0 78-79 0.0 0.0 0.0 1.4026374530724934 0.0 80-81 0.0 0.0 0.0 1.6199474810133023 0.0 82-83 0.0 0.0 0.0 1.8598667778694635 0.0 84-85 0.0 0.0 0.0 2.112418063522754 0.0 86-87 0.0 0.0 0.0 2.4610698033524545 0.0 88-89 0.0 0.0 0.0 2.85436489413243 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4670 0.0 47.81919 1 GTATCAA 7750 0.0 41.68967 1 ATCAACG 10095 0.0 31.576952 3 TATCAAC 10245 0.0 31.068254 2 TCAACGC 10310 0.0 30.872381 4 CAACGCA 10510 0.0 30.284895 5 AACGCAG 10690 0.0 29.85855 6 CTACGCG 40 4.4932088E-4 29.690315 90-91 ACGCAGA 12155 0.0 26.182804 7 CGCAGAG 12310 0.0 25.621609 8 TACGCGC 50 0.0016546528 23.749361 92-93 GCAGAGT 13410 0.0 23.201115 9 GTACATG 6485 0.0 23.079256 1 GAGTACT 8630 0.0 22.676601 12-13 TACATGG 6565 0.0 22.505032 2 AGAGTAC 11640 0.0 22.219858 10-11 AGTACTT 9005 0.0 21.705893 12-13 ACATGGG 6725 0.0 21.26318 3 GTACTTT 9290 0.0 21.038832 14-15 CAGAGTA 12380 0.0 20.891691 10-11 >>END_MODULE