FastQCFastQC Report
Thu 26 May 2016
SRR937573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215049
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.31674242872279573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54530.2461796556193565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35490.16022218921567874No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32890.1484842998958488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.045.043661
GTATCAA69000.039.6268961
TATCAAC85900.031.5438962
ATCAACG85950.031.3007743
TCAACGC87150.030.923624
CAACGCA88750.030.2562735
AACGCAG91200.029.4815676
ACGCAGA105800.025.1438247
CGCAGAG106950.024.6513778
GTACATG66800.023.918511
GTACCGT1801.9511936E-723.7519346
TACCGTA1452.3341354E-522.9329017
TACATGG67950.022.7367062
ACATGGG66700.022.661353
GAGTACT68200.022.38988712-13
GCAGAGT117200.022.0901099
TATCCCG1553.891779E-521.4635565
ACCGTAT2056.8297595E-720.8553568
AGTACTT71950.020.82686412-13
GTGTACG2308.925781E-820.6749041
Produced by FastQC (version 0.11.5)