##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937573_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2215049 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.730201002325458 33.0 31.0 34.0 30.0 34.0 2 31.87139246129544 33.0 31.0 34.0 30.0 34.0 3 31.90424229892883 33.0 31.0 34.0 30.0 34.0 4 35.51711903438705 37.0 35.0 37.0 33.0 37.0 5 35.49639759662202 37.0 35.0 37.0 33.0 37.0 6 35.54824656249139 37.0 35.0 37.0 33.0 37.0 7 35.48222860984114 37.0 35.0 37.0 33.0 37.0 8 35.45772396005687 37.0 35.0 37.0 33.0 37.0 9 37.09003367419863 39.0 37.0 39.0 33.0 39.0 10-11 36.97873771641169 39.0 37.0 39.0 33.0 39.0 12-13 36.8485114776242 39.0 37.0 39.0 33.0 39.0 14-15 38.02565360856577 40.0 38.0 41.0 33.0 41.0 16-17 37.94309967860756 40.0 38.0 41.0 33.0 41.0 18-19 37.84372061295258 40.0 38.0 41.0 33.0 41.0 20-21 37.74059986934826 40.0 37.5 41.0 32.0 41.0 22-23 37.541411499248994 40.0 37.0 41.0 32.0 41.0 24-25 37.36995863296929 39.5 37.0 41.0 32.0 41.0 26-27 37.311488143151685 39.0 37.0 41.0 32.0 41.0 28-29 37.34791893994219 40.0 37.0 41.0 32.0 41.0 30-31 37.185441044419335 39.0 36.5 41.0 31.0 41.0 32-33 36.96646439875597 39.0 36.0 41.0 31.0 41.0 34-35 37.00688224052831 39.0 36.0 41.0 31.0 41.0 36-37 36.93140377481491 39.0 36.0 41.0 31.0 41.0 38-39 36.8108436878823 39.0 36.0 41.0 30.0 41.0 40-41 36.57841113221423 39.0 35.5 41.0 30.0 41.0 42-43 36.33327840603074 39.0 35.0 41.0 30.0 41.0 44-45 36.11180678170099 39.0 35.0 41.0 29.5 41.0 46-47 35.793844966860775 39.0 35.0 40.0 28.5 41.0 48-49 35.45491702440894 39.0 35.0 40.0 27.0 41.0 50-51 34.48696981421179 37.5 34.0 39.5 25.0 40.5 52-53 34.56523512572409 38.0 34.0 39.5 26.0 40.5 54-55 34.89070286932704 38.0 34.0 40.0 26.0 41.0 56-57 34.65881928571332 38.0 34.0 40.0 26.0 41.0 58-59 34.378706520713536 38.0 34.0 40.0 25.0 41.0 60-61 34.01875805004765 37.0 33.5 40.0 24.5 41.0 62-63 33.62733194615559 36.5 33.0 39.5 23.0 41.0 64-65 33.21958475862159 36.0 32.5 39.0 22.0 41.0 66-67 32.78279306688023 35.5 32.0 39.0 21.5 40.0 68-69 32.27710086774604 35.0 31.5 38.5 20.0 40.0 70-71 31.820027457631863 35.0 31.0 37.5 18.5 40.0 72-73 31.366717846873815 35.0 31.0 37.0 17.5 39.0 74-75 30.912850686373076 35.0 30.5 36.5 14.0 39.0 76-77 30.42528111116278 34.0 30.0 36.0 9.5 38.5 78-79 29.91302449742647 34.0 29.5 35.5 7.0 37.0 80-81 29.41323668234879 34.0 29.0 35.0 3.5 37.0 82-83 28.912914341849774 34.0 29.0 35.0 2.0 36.0 84-85 28.477898683053965 34.0 28.0 35.0 2.0 36.0 86-87 28.020024387722348 33.0 27.0 35.0 2.0 35.5 88-89 27.5500810591549 33.0 26.0 35.0 2.0 35.0 90-91 27.055480262513377 33.0 25.5 35.0 2.0 35.0 92-93 26.477956469586 32.0 25.0 35.0 2.0 35.0 94-95 25.8254839960651 32.0 23.5 34.0 2.0 35.0 96-97 25.07367850553193 32.0 19.5 34.0 2.0 35.0 98-99 24.083929746023678 31.0 8.5 34.0 2.0 35.0 100-101 21.666464940504703 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21557739352701688 1101 2 0.256805536817712 1101 3 0.21805190783149797 1101 4 0.12592979531964943 1101 5 0.11798534949344486 1101 6 0.19860972596641346 1101 7 0.17342964186192944 1101 8 0.13611473770917115 1101 9 0.18235099068451888 1101 10-11 0.15966104024001737 1101 12-13 0.32445380894733233 1101 14-15 0.48818638160840067 1101 16-17 0.5997202264606045 1101 18-19 0.49286438902688445 1101 20-21 0.5409621400078422 1101 22-23 0.46895960608880927 1101 24-25 0.4684016340140147 1101 26-27 0.5442518251760831 1101 28-29 0.34090505650427616 1101 30-31 0.4423713870502155 1101 32-33 0.38396476795860934 1101 34-35 0.5905422151086341 1101 36-37 0.5157740929851684 1101 38-39 0.662899579033656 1101 40-41 0.6263707299116064 1101 42-43 0.6470760429677469 1101 44-45 0.7013924710940032 1101 46-47 0.8479151744069924 1101 48-49 0.7607552166576781 1101 50-51 0.687321502492253 1101 52-53 0.6861609434843245 1101 54-55 0.6996552269369758 1101 56-57 0.7181944970702787 1101 58-59 0.8336491862665767 1101 60-61 0.7853744223185899 1101 62-63 0.9852601548734725 1101 64-65 0.7584112608911155 1101 66-67 0.8896195844176589 1101 68-69 0.8553900380423372 1101 70-71 0.8419808833817513 1101 72-73 0.8779891790900685 1101 74-75 0.9315392868763617 1101 76-77 1.0650660098770466 1101 78-79 1.17845137189612 1101 80-81 1.3035951751384616 1101 82-83 1.1770737056302636 1101 84-85 1.3409849820395472 1101 86-87 1.4768705752318994 1101 88-89 1.6137373076942865 1101 90-91 1.6180252133536754 1101 92-93 1.6467301077714787 1101 94-95 1.708014528878234 1101 96-97 1.7707149442446841 1101 98-99 2.041148966812404 1101 100-101 2.319179802244193 1102 1 0.22785650085699416 1102 2 0.2629754030862905 1102 3 0.36895917239990794 1102 4 0.2716850296666635 1102 5 0.1835966555198496 1102 6 0.19756741735852756 1102 7 0.19792815830563626 1102 8 0.329584049488858 1102 9 0.35189411022407313 1102 10-11 0.3016458316559323 1102 12-13 0.4609624014117131 1102 14-15 0.5410292217899766 1102 16-17 0.5424655583196554 1102 18-19 0.5194133167208506 1102 20-21 0.6034391042830904 1102 22-23 0.6823925019565777 1102 24-25 0.6711224080291984 1102 26-27 0.531462502035204 1102 28-29 0.4219823719978564 1102 30-31 0.5603400681052619 1102 32-33 0.5987005495862405 1102 34-35 0.6117364723627006 1102 36-37 0.7375097685650402 1102 38-39 0.6481769235976813 1102 40-41 0.6286366665543639 1102 42-43 0.6685823253707142 1102 44-45 0.7197490236962949 1102 46-47 0.6019449894018578 1102 48-49 0.5822300184484703 1102 50-51 0.4601927790477731 1102 52-53 0.4835244329451811 1102 54-55 0.5148618087209442 1102 56-57 0.5645892253840614 1102 58-59 0.41353466951436957 1102 60-61 0.5508534096588491 1102 62-63 0.8096670289932888 1102 64-65 0.8301741714646766 1102 66-67 0.7675900341714055 1102 68-69 0.7887283585869085 1102 70-71 0.9160431022717503 1102 72-73 0.8717153044385206 1102 74-75 0.8379297028283155 1102 76-77 0.8494667361929835 1102 78-79 0.8504762315682335 1102 80-81 1.0037245459934212 1102 82-83 0.8563540306555346 1102 84-85 1.039099908892549 1102 86-87 1.1777519211337477 1102 88-89 1.1045247790780728 1102 90-91 1.1432628196702694 1102 92-93 1.2405305592531448 1102 94-95 1.5971864589994347 1102 96-97 1.6861465808879679 1102 98-99 1.869130039882279 1102 100-101 2.517508307808736 1103 1 0.37040189682052116 1103 2 0.3233352580279565 1103 3 0.34980922603382325 1103 4 0.2618136659810517 1103 5 0.27194617357093165 1103 6 0.27119592348799415 1103 7 0.2994084846887759 1103 8 0.3032227851087157 1103 9 0.3065528867581193 1103 10-11 0.31419605495568703 1103 12-13 0.4658372924752001 1103 14-15 0.557788568395928 1103 16-17 0.4968690703060261 1103 18-19 0.3747343818509421 1103 20-21 0.2631894253018956 1103 22-23 0.4777186459057461 1103 24-25 0.5609073069945509 1103 26-27 0.39106775715698916 1103 28-29 0.25299922683230847 1103 30-31 0.28634297887744253 1103 32-33 0.2712796158890356 1103 34-35 0.5149042297653281 1103 36-37 0.6091757063096068 1103 38-39 0.6292291089363786 1103 40-41 0.7555584540642215 1103 42-43 0.6958256705766885 1103 44-45 0.7335824713314238 1103 46-47 0.6010953366278216 1103 48-49 0.549814635337043 1103 50-51 0.6075371485074612 1103 52-53 0.48265123910730523 1103 54-55 0.6356541271867187 1103 56-57 0.6441913946411049 1103 58-59 0.5659901915577876 1103 60-61 0.7905918221137966 1103 62-63 0.8784177132440902 1103 64-65 0.9063999470954514 1103 66-67 0.762594696058386 1103 68-69 0.8312532577526781 1103 70-71 0.8036924769397871 1103 72-73 0.8387110165613798 1103 74-75 0.7472394088751173 1103 76-77 0.8444631728889824 1103 78-79 1.0116908256664026 1103 80-81 0.7567759500335214 1103 82-83 0.9350008487073111 1103 84-85 1.0620410065036303 1103 86-87 1.0373382677705223 1103 88-89 1.0304535307720357 1103 90-91 1.0215997654696594 1103 92-93 1.3103971277469562 1103 94-95 1.3503299473615478 1103 96-97 1.5915300345643537 1103 98-99 2.003243386039344 1103 100-101 2.46324067219566 1104 1 0.09186419939413781 1104 2 0.18064807795735405 1104 3 0.21681646757185646 1104 4 0.2388131508943232 1104 5 0.22342366524286206 1104 6 0.18400384883869236 1104 7 0.21794322083701445 1104 8 0.23378813200592674 1104 9 0.3566522338757565 1104 10-11 0.348849492349224 1104 12-13 0.21021823581377674 1104 14-15 0.44040703677519133 1104 16-17 0.5847686311125031 1104 18-19 0.5908661746225405 1104 20-21 0.5678125116856805 1104 22-23 0.558829016464955 1104 24-25 0.5415873720506497 1104 26-27 0.6247026696225646 1104 28-29 0.37220311564301056 1104 30-31 0.5399104720739274 1104 32-33 0.48735891707758583 1104 34-35 0.5172350860954467 1104 36-37 0.6554305155110711 1104 38-39 0.674565210869595 1104 40-41 0.6409745565160776 1104 42-43 0.604903033759264 1104 44-45 0.6591930101355601 1104 46-47 0.8572710968512922 1104 48-49 0.6293210847959045 1104 50-51 0.5688996836125142 1104 52-53 0.5295034276194315 1104 54-55 0.6257715933113488 1104 56-57 0.6703009380918985 1104 58-59 0.6445404995118906 1104 60-61 0.7364693402197346 1104 62-63 0.9076930638031655 1104 64-65 0.6558208143609718 1104 66-67 0.6396676691879293 1104 68-69 0.8579803615410881 1104 70-71 0.7592538555988071 1104 72-73 0.7893278344881587 1104 74-75 0.7678039799007266 1104 76-77 0.8424102500802597 1104 78-79 0.8819647794474577 1104 80-81 0.8727898772425426 1104 82-83 0.9604715005795796 1104 84-85 1.052256031685996 1104 86-87 1.2081214108201266 1104 88-89 0.96511363940661 1104 90-91 1.1098630030208412 1104 92-93 1.3473800621268346 1104 94-95 1.545335446429977 1104 96-97 1.6453101709546338 1104 98-99 1.8338379901973916 1104 100-101 2.002824972318688 1105 1 0.2580240459126344 1105 2 0.1850818356576447 1105 3 0.3170019995101008 1105 4 0.26732564233300593 1105 5 0.2396487688360196 1105 6 0.2700006212590509 1105 7 0.31476718056967457 1105 8 0.303623735129257 1105 9 0.29769378969847793 1105 10-11 0.4188396645945929 1105 12-13 0.43551902095632755 1105 14-15 0.5104852717770854 1105 16-17 0.6144336545942721 1105 18-19 0.47427794382413424 1105 20-21 0.5443843890219782 1105 22-23 0.6504751555683441 1105 24-25 0.552160169452911 1105 26-27 0.5549720314260114 1105 28-29 0.6678199909657465 1105 30-31 0.5810164276148484 1105 32-33 0.6509311393161283 1105 34-35 0.6586126766592386 1105 36-37 0.6293905176275132 1105 38-39 0.7101701223953043 1105 40-41 0.8716926096996147 1105 42-43 0.7765500841100916 1105 44-45 0.7319375012528582 1105 46-47 0.6241512207809379 1105 48-49 0.7289538667755551 1105 50-51 0.7055576493358942 1105 52-53 0.5914696007427267 1105 54-55 0.6487377233218652 1105 56-57 0.6730616258975388 1105 58-59 0.6640227003018353 1105 60-61 0.6180282396287922 1105 62-63 0.7554873060911262 1105 64-65 0.8532847049564083 1105 66-67 0.9448478659193853 1105 68-69 0.973463459860767 1105 70-71 1.061837732006218 1105 72-73 1.0293140334987179 1105 74-75 1.0607312055246112 1105 76-77 1.0845414324777032 1105 78-79 1.2696056431140619 1105 80-81 1.3254042755399809 1105 82-83 1.2749930858757708 1105 84-85 1.4534294097390443 1105 86-87 1.3728359993037245 1105 88-89 1.3509162083405215 1105 90-91 1.567343634113584 1105 92-93 1.7593687469812131 1105 94-95 1.8084456515194383 1105 96-97 1.879102459449694 1105 98-99 2.1252425507458668 1105 100-101 2.3256919429304723 1106 1 0.26607416338469747 1106 2 0.2789530692502744 1106 3 0.33681469453323487 1106 4 0.288026324463182 1106 5 0.277503505496405 1106 6 0.11563807421406125 1106 7 0.20259643637498925 1106 8 0.13889651034318007 1106 9 0.22597523313275047 1106 10-11 0.09553254520820786 1106 12-13 0.06262385191362085 1106 14-15 0.10104798483191502 1106 16-17 0.20191634712252693 1106 18-19 0.3744201872981421 1106 20-21 0.4698534791485329 1106 22-23 0.5337694399346873 1106 24-25 0.29421632074415527 1106 26-27 0.4608310614035602 1106 28-29 0.5428276766064855 1106 30-31 0.6106320096011331 1106 32-33 0.6818438209780737 1106 34-35 0.6176251545925808 1106 36-37 0.5262937069319449 1106 38-39 0.6957401841050839 1106 40-41 0.7657572849576368 1106 42-43 0.80949709575561 1106 44-45 0.9025309391345573 1106 46-47 0.8034303195035832 1106 48-49 0.9428453963717729 1106 50-51 0.8770358227073984 1106 52-53 0.9774973127984197 1106 54-55 1.01311661122147 1106 56-57 0.8360161280867686 1106 58-59 0.9707993366634327 1106 60-61 0.9000008007089448 1106 62-63 1.146343521941752 1106 64-65 1.184376196006184 1106 66-67 1.1712018097763774 1106 68-69 1.1884924642865116 1106 70-71 1.2659266702408587 1106 72-73 1.4000112570905578 1106 74-75 1.6803515526081476 1106 76-77 1.7782154407048871 1106 78-79 1.7151140068647983 1106 80-81 1.7782165106070735 1106 82-83 1.434093091433077 1106 84-85 1.2884832919926659 1106 86-87 1.6141506340643375 1106 88-89 1.608312455146546 1106 90-91 1.8327100381678214 1106 92-93 1.8784618886701416 1106 94-95 2.2832896625725745 1106 96-97 2.558715883544533 1106 98-99 2.8955097331781445 1106 100-101 3.2421499148908914 1107 1 0.3534676225897151 1107 2 0.3253303043508673 1107 3 0.3585530325042434 1107 4 0.24390801057508327 1107 5 0.2394693419062932 1107 6 0.21917176554086382 1107 7 0.2986483032450451 1107 8 0.3050481378363443 1107 9 0.33709062512315313 1107 10-11 0.4195820838068087 1107 12-13 0.30673211890731267 1107 14-15 0.5844928367172813 1107 16-17 0.5120528633752315 1107 18-19 0.6247902775453369 1107 20-21 0.6509723119779878 1107 22-23 0.6403263870065175 1107 24-25 0.7641540270352039 1107 26-27 0.542392135407674 1107 28-29 0.6689028647190227 1107 30-31 0.7283177445225562 1107 32-33 0.6247250035597887 1107 34-35 0.6250195503222642 1107 36-37 0.5376809281713761 1107 38-39 0.6785395452530096 1107 40-41 0.6989941898384515 1107 42-43 0.9306841289195447 1107 44-45 0.7491329401558389 1107 46-47 0.9511060342037894 1107 48-49 1.0135476742902085 1107 50-51 0.9704350427246027 1107 52-53 1.0550108812923114 1107 54-55 1.139216546109303 1107 56-57 1.227636958884915 1107 58-59 1.4485267943516362 1107 60-61 1.5246923921696194 1107 62-63 1.4697740947895923 1107 64-65 1.4857974018291173 1107 66-67 1.4822997456582243 1107 68-69 1.4629515397884774 1107 70-71 1.6601048653977841 1107 72-73 1.6873272455523356 1107 74-75 1.6953528403295728 1107 76-77 1.6009256897522839 1107 78-79 1.7646597183079145 1107 80-81 1.6714245432621482 1107 82-83 1.7306291059930743 1107 84-85 1.9143536894258766 1107 86-87 1.9431904265826319 1107 88-89 2.0283769627286894 1107 90-91 1.9620566906996828 1107 92-93 1.899335719533191 1107 94-95 2.159860008467291 1107 96-97 2.345566276988624 1107 98-99 2.5375993449560674 1107 100-101 2.9318329101812957 1108 1 0.24080665898081577 1108 2 0.2584539970652493 1108 3 0.3355464495659568 1108 4 0.20895399469777942 1108 5 0.1592750923464621 1108 6 0.33114172064449576 1108 7 0.3226530400234111 1108 8 0.24790074807850715 1108 9 0.4046497211459368 1108 10-11 0.36270074054326784 1108 12-13 0.4477101836341575 1108 14-15 0.5486878008489455 1108 16-17 0.5788543707711042 1108 18-19 0.5659797547078043 1108 20-21 0.63234713943136 1108 22-23 0.681451530783967 1108 24-25 0.6618710686420286 1108 26-27 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-1.3912389558711382 2316 96-97 -1.3612279173296749 2316 98-99 -1.2933694398124942 2316 100-101 -0.9750057071327696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8175.0 3 1303.0 4 1338.0 5 1681.0 6 1956.0 7 2290.0 8 2748.0 9 3479.0 10 4443.0 11 5610.0 12 7533.0 13 9302.0 14 10514.0 15 12815.0 16 15888.0 17 19950.0 18 18132.0 19 14310.0 20 14848.0 21 15847.0 22 17729.0 23 19691.0 24 22347.0 25 25706.0 26 29608.0 27 35361.0 28 41683.0 29 50722.0 30 62149.0 31 77516.0 32 98320.0 33 126983.0 34 172376.0 35 239223.0 36 336529.0 37 413337.0 38 251819.0 39 21787.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.492546345281404 17.973109590828056 14.312638601017493 24.221705462873043 2 15.823056234707755 19.28769098466779 39.24184424880232 25.647408531822137 3 19.248666645234582 22.280551698032216 29.68304213622926 28.787739520503937 4 13.457251460505681 15.498835579475776 34.96838136492541 36.07553159509314 5 14.758347129587 36.07415888123361 33.555612596669974 15.611881392509416 6 34.56916874431569 34.07523879766338 16.289100147961793 15.06649231005914 7 29.870217420319563 31.902399518603225 19.57805391734363 18.649329143733585 8 27.140455628383897 34.2623585070014 19.085756691999467 19.511429172615237 9 26.574367031328055 16.233058087591136 19.646690534973466 37.54588434610734 10-11 25.801539705588887 25.535555180476248 26.46021114953914 22.202693964395724 12-13 26.060109284672272 22.74331133642955 27.319086627740713 23.87749275115746 14-15 23.185177811512332 24.45482372690396 25.107428967021335 27.25256949456238 16-17 23.233575526020253 27.67136663460155 25.809674374662343 23.285383464715853 18-19 23.18928812209568 26.21482577474922 27.83977430579122 22.756111797363886 20-21 23.528943383610052 25.497326914489765 27.97720921457839 22.99652048732179 22-23 23.486436413783867 25.05553433176383 28.07514081778474 23.382888436667564 24-25 23.48423794749398 25.294921414229464 27.955168552210957 23.265672086065596 26-27 23.119452385385213 25.724417772636055 27.831224701565283 23.324905140413453 28-29 23.055639207981095 25.918068086764322 27.942258181075236 23.084034524179348 30-31 23.26010309427624 25.50891034273055 27.85095221030074 23.38003435269247 32-33 23.142645882280537 25.50763304891161 27.83745968077674 23.51226138803112 34-35 23.623265196050042 25.525011221475737 27.549650505328394 23.302073077145835 36-37 23.652262189962116 25.51648758807936 27.435249750558206 23.39600047140032 38-39 23.206871123456736 25.590546550187522 27.636230382598704 23.56635194375704 40-41 23.434878335543193 25.580568972295094 27.547118428153755 23.43743426400796 42-43 23.43985482034198 25.6083651136286 27.320587432874543 23.631192633154875 44-45 23.315175382460467 25.737939621137418 27.33609717840328 23.610787817998833 46-47 23.492475696584798 26.058735374122733 26.848252812070555 23.600536117221917 48-49 23.774388424365426 25.901043418810225 26.667437374170877 23.65713078265347 50-51 23.547922319342153 26.249192259447167 26.628015790988417 23.57486963022226 52-53 23.752002096345922 26.137862804297146 26.427762281975724 23.682372817381207 54-55 23.60825467595074 26.028322313419515 26.452657052152883 23.910765958476865 56-57 23.51141785687955 26.445703270056843 26.52700577462629 23.515873098437318 58-59 23.712222894393154 26.074686356877535 26.450571879258 23.762518869471315 60-61 23.47693062042749 26.06854366314662 26.919560912371548 23.534964804054344 62-63 23.35821732765625 26.15949883376068 26.889891265600376 23.592392572982696 64-65 23.37681634683885 26.21776938473194 26.669885676161613 23.735528592267592 66-67 23.348123037491753 26.19781562922739 26.559766440738613 23.89429489254225 68-69 23.155809314599516 26.08209551912425 26.96842259068703 23.793672575589206 70-71 23.18517818242348 26.13686736916764 26.79114226368393 23.886812184724945 72-73 23.344952700009998 26.198128446625173 26.442216569856203 24.014702283508626 74-75 23.256868594796448 26.609977811504095 26.283935845616924 23.849217748082538 76-77 23.48784420750495 26.362849523713 26.359139489155385 23.790166779626663 78-79 23.516818395714182 26.333730000723953 26.36879660464779 23.780654998914066 80-81 23.430538794151612 26.367875712131887 26.43641681586987 23.76516867784663 82-83 23.439892453878834 26.378500185826052 26.421750266524096 23.759857093771018 84-85 23.41834610179773 26.27701796285561 26.266161130322928 24.03847480502373 86-87 23.52184232306204 26.188010161439863 26.42001507794456 23.870132437553536 88-89 23.49185961604062 26.285708339629505 26.2235006591752 23.998931385154666 90-91 23.434089164182495 26.31155933036153 26.31386668126481 23.94048482419117 92-93 23.368597965101813 25.999669747515192 26.492809091443746 24.138923195939253 94-95 23.3333906377804 26.076607299259507 26.44814369783787 24.14185836512222 96-97 23.445334703627825 26.030446230792997 26.313906302796784 24.21031276278239 98-99 23.1977277837115 26.18570034593529 26.25532168996652 24.361250180386687 100-101 23.609891707952983 25.544592612294025 26.561993250349087 24.28352242940391 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2980.0 1 1747.0 2 481.5 3 449.5 4 399.5 5 398.5 6 573.5 7 1111.5 8 1808.5 9 2173.0 10 2074.0 11 1964.0 12 2121.5 13 2294.0 14 2480.5 15 2611.0 16 2679.5 17 2809.0 18 2952.0 19 3152.5 20 3498.5 21 3983.5 22 4757.0 23 5552.5 24 6461.5 25 8115.5 26 9887.0 27 11973.5 28 14274.5 29 16436.5 30 18475.0 31 20979.0 32 23767.5 33 25170.0 34 26647.0 35 28555.0 36 31304.5 37 35860.0 38 41378.0 39 45399.5 40 48845.5 41 54698.0 42 60370.5 43 64972.5 44 70600.0 45 76880.0 46 81355.5 47 85078.5 48 89283.5 49 91579.5 50 91838.0 51 90568.5 52 90543.5 53 92908.5 54 95179.0 55 90697.0 56 81835.5 57 74979.0 58 69018.5 59 61589.0 60 51156.0 61 41804.5 62 34066.0 63 26562.5 64 20589.0 65 15931.0 66 11637.5 67 8997.5 68 6605.5 69 4257.0 70 3191.5 71 2381.0 72 1810.0 73 1418.5 74 1084.5 75 822.5 76 557.5 77 366.5 78 241.5 79 149.5 80 97.0 81 68.0 82 44.0 83 31.5 84 23.0 85 17.0 86 17.5 87 11.5 88 7.0 89 5.0 90 4.5 91 7.0 92 6.5 93 4.0 94 2.5 95 3.0 96 2.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32834488085816615 2 0.30798415746107644 3 0.29444043901511885 4 0.2810321577536208 5 0.27611127338492286 6 0.22658640960087112 7 0.21733153532946678 8 0.23177816833848822 9 0.23227477134817334 10-11 0.23231991707632652 12-13 0.22757961562024134 14-15 0.2250063091155094 16-17 0.22405824882429237 18-19 0.22478058047474345 20-21 0.2113722992132454 22-23 0.20223028926222403 24-25 0.20326864100974742 26-27 0.2170832338246242 28-29 0.2337420075131521 30-31 0.22721844979501582 32-33 0.23408060047430101 34-35 0.22577378649411367 36-37 0.20827981683475172 38-39 0.20943103290265813 40-41 0.2032460681456708 42-43 0.2176927011546923 44-45 0.21320070120344967 46-47 0.21270409819376454 48-49 0.223855093047603 50-51 0.23390001756168824 52-53 0.24785004756102463 54-55 0.2204691634361136 56-57 0.18825768639881105 58-59 0.1867453045056791 60-61 0.19335915368012174 62-63 0.20428441989319424 64-65 0.19069555571908342 66-67 0.19816717372843673 68-69 0.20159824906807933 70-71 0.19326886222381537 72-73 0.19737712348575584 74-75 0.20062761591278566 76-77 0.21791842979545828 78-79 0.22432912319321152 80-81 0.21202691227146667 82-83 0.20985991732011344 84-85 0.20132737469916018 86-87 0.20475845003880272 88-89 0.21268152532968798 90-91 0.21317812833937308 92-93 0.2085281183395943 94-95 0.20916015853373898 96-97 0.19557129435962814 98-99 0.2040586912524283 100-101 0.19268196775782387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2215049.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.794056960866236 #Duplication Level Percentage of deduplicated Percentage of total 1 64.73622985787053 25.76117218395829 2 15.564322214716475 12.387350495394061 3 6.955167882788809 8.303230407002557 4 3.770751322319722 6.002139716226107 5 2.2232587367226246 4.423624240394182 6 1.5042783681596628 3.5916803440526714 7 1.0340601418043358 2.8804613728746213 8 0.6994793904250838 2.226809816442222 9 0.5264806742131274 1.8855721744589202 >10 2.7118776652465946 19.278028401644303 >50 0.17848972474823055 4.903641046734171 >100 0.09128114567091186 6.386515914312151 >500 0.003526556177155036 0.9070231106367936 >1k 5.687993834121008E-4 0.4982832590726274 >5k 2.2751975336484035E-4 0.5644675167962743 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7016 0.31674242872279573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5453 0.2461796556193565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3549 0.16022218921567874 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3289 0.1484842998958488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.5145728153192096E-5 0.0 6 0.0 0.0 0.0 4.5145728153192096E-5 0.0 7 0.0 0.0 0.0 4.5145728153192096E-5 0.0 8 0.0 0.0 0.0 4.5145728153192096E-5 0.0 9 0.0 0.0 0.0 4.5145728153192096E-5 0.0 10-11 0.0 0.0 0.0 4.5145728153192096E-5 0.0 12-13 0.0 0.0 0.0 4.5145728153192096E-5 0.0 14-15 0.0 0.0 0.0 4.5145728153192096E-5 0.0 16-17 0.0 0.0 0.0 4.5145728153192096E-5 0.0 18-19 0.0 0.0 0.0 4.5145728153192096E-5 0.0 20-21 0.0 0.0 0.0 1.354371844595763E-4 0.0 22-23 0.0 0.0 0.0 1.5801004853617235E-4 0.0 24-25 0.0 0.0 0.0 3.385929611489407E-4 0.0 26-27 0.0 0.0 0.0 5.417487378383051E-4 0.0 28-29 0.0 0.0 0.0 0.0015575276212851275 0.0 30-31 0.0 0.0 0.0 0.004356562766783038 0.0 32-33 0.0 0.0 0.0 0.008690552669489478 0.0 34-35 0.0 0.0 0.0 0.012663376746970384 0.0 36-37 0.0 0.0 0.0 0.016884502329293845 0.0 38-39 0.0 0.0 0.0 0.022189125387293916 0.0 40-41 0.0 0.0 0.0 0.02848695446466421 0.0 42-43 0.0 0.0 0.0 0.03789983878460476 0.0 44-45 0.0 0.0 0.0 0.046409808541481475 0.0 46-47 0.0 0.0 0.0 0.05627415014295395 0.0 48-49 0.0 0.0 0.0 0.0668382505308009 0.0 50-51 0.0 0.0 0.0 0.08207493378250323 0.0 52-53 0.0 0.0 0.0 0.118800983635125 0.0 54-55 0.0 0.0 0.0 0.17218580717627466 0.0 56-57 0.0 0.0 0.0 0.2181893041643774 0.0 58-59 0.0 0.0 0.0 0.2796100673167953 0.0 60-61 0.0 0.0 0.0 0.3513692022162941 0.0 62-63 0.0 0.0 0.0 0.4016841162430267 0.0 64-65 0.0 0.0 0.0 0.4469652815806784 0.0 66-67 0.0 0.0 0.0 0.5655856823031906 0.0 68-69 0.0 0.0 0.0 0.6819939423461964 0.0 70-71 0.0 0.0 0.0 0.7444079115179845 0.0 72-73 0.0 0.0 0.0 0.8632088951531095 0.0 74-75 0.0 0.0 0.0 1.031873335533435 0.0 76-77 0.0 0.0 0.0 1.2045783185834715 0.0 78-79 0.0 0.0 0.0 1.3765158242549036 0.0 80-81 0.0 0.0 0.0 1.5935764852154513 0.0 82-83 0.0 0.0 0.0 1.8316976283594628 0.0 84-85 0.0 0.0 0.0 2.081579233687381 0.0 86-87 0.0 0.0 0.0 2.425634827942858 0.0 88-89 0.0 0.0 0.0 2.814407265934072 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4265 0.0 45.04366 1 GTATCAA 6900 0.0 39.626896 1 TATCAAC 8590 0.0 31.543896 2 ATCAACG 8595 0.0 31.300774 3 TCAACGC 8715 0.0 30.92362 4 CAACGCA 8875 0.0 30.256273 5 AACGCAG 9120 0.0 29.481567 6 ACGCAGA 10580 0.0 25.143824 7 CGCAGAG 10695 0.0 24.651377 8 GTACATG 6680 0.0 23.91851 1 GTACCGT 180 1.9511936E-7 23.751934 6 TACCGTA 145 2.3341354E-5 22.932901 7 TACATGG 6795 0.0 22.736706 2 ACATGGG 6670 0.0 22.66135 3 GAGTACT 6820 0.0 22.389887 12-13 GCAGAGT 11720 0.0 22.090109 9 TATCCCG 155 3.891779E-5 21.463556 5 ACCGTAT 205 6.8297595E-7 20.855356 8 AGTACTT 7195 0.0 20.826864 12-13 GTGTACG 230 8.925781E-8 20.674904 1 >>END_MODULE