FastQCFastQC Report
Thu 26 May 2016
SRR937573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215049
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.4156567191064396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75710.34179830784781734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51540.23268108290155207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.048.0709421
GTATCAA77600.039.1334341
TATCAAC100000.030.0226752
ATCAACG99900.030.0051773
TCAACGC101950.029.448434
CAACGCA103000.029.1943475
AACGCAG106000.028.409066
ACGCAGA120600.024.7351887
CGCAGAG121550.024.307398
GAGTACT82350.022.12099512-13
GCAGAGT134150.021.811879
GTACATG62800.021.0374931
AGAGTAC115550.020.7804110-11
TAAACCG1150.0037224320.6540155
AGTACTT85850.020.2508712-13
CAGAGTA124900.020.1375410-11
GTACTTT90350.020.05579414-15
TACATGG65200.019.9634342
ACATGGG64650.018.6636893
AATTCGC5650.017.65484678-79
Produced by FastQC (version 0.11.5)