##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937573_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2215049 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.223374742500056 33.0 31.0 34.0 31.0 34.0 2 32.432245968373614 34.0 31.0 34.0 31.0 34.0 3 32.50275727534696 34.0 31.0 34.0 31.0 34.0 4 36.11561459814207 37.0 35.0 37.0 35.0 37.0 5 36.02336381723384 37.0 35.0 37.0 35.0 37.0 6 36.0649168483406 37.0 35.0 37.0 35.0 37.0 7 36.011842627409145 37.0 35.0 37.0 35.0 37.0 8 35.97619149734385 37.0 35.0 37.0 35.0 37.0 9 37.67009849443511 39.0 37.0 39.0 35.0 39.0 10-11 37.591542444433514 39.0 37.0 39.0 35.0 39.0 12-13 37.48120921929944 39.0 37.0 39.0 35.0 39.0 14-15 38.73477313594417 40.0 38.0 41.0 35.0 41.0 16-17 38.68328894755827 40.0 38.0 41.0 34.5 41.0 18-19 38.63134969023258 40.0 38.0 41.0 34.0 41.0 20-21 38.50862531709231 40.0 38.0 41.0 34.0 41.0 22-23 38.45142026203484 40.0 38.0 41.0 34.0 41.0 24-25 38.46035618173684 40.0 38.0 41.0 34.0 41.0 26-27 38.27778189105523 40.0 38.0 41.0 34.0 41.0 28-29 38.2376337950086 40.0 38.0 41.0 34.0 41.0 30-31 38.26841505537801 40.0 38.0 41.0 34.0 41.0 32-33 38.154456176815955 40.0 38.0 41.0 34.0 41.0 34-35 38.004796959344915 40.0 37.0 41.0 33.0 41.0 36-37 37.840974849766305 40.0 37.0 41.0 33.0 41.0 38-39 37.68790667836242 40.0 37.0 41.0 33.0 41.0 40-41 37.50912259728791 40.0 37.0 41.0 33.0 41.0 42-43 37.279874847012415 40.0 36.5 41.0 32.0 41.0 44-45 36.977504109389905 39.5 36.0 41.0 31.0 41.0 46-47 36.6398930678283 39.0 36.0 41.0 30.5 41.0 48-49 36.30549933658352 39.0 35.0 41.0 30.0 41.0 50-51 36.04071760940729 39.0 35.0 40.0 29.5 41.0 52-53 35.745662285574724 39.0 35.0 40.0 28.5 41.0 54-55 35.48468498890995 38.0 35.0 40.0 28.0 41.0 56-57 35.17210928516705 38.0 34.0 40.0 28.0 41.0 58-59 34.8776929991165 38.0 34.0 40.0 27.0 41.0 60-61 34.50244825283775 37.0 34.0 40.0 26.0 41.0 62-63 34.06379791146833 36.5 33.5 39.5 26.0 41.0 64-65 33.63375528035723 36.0 33.0 39.0 25.5 40.5 66-67 33.189226739453616 35.5 32.5 39.0 24.5 40.0 68-69 32.71185942162002 35.0 32.0 38.0 23.0 40.0 70-71 32.275646046656306 35.0 32.0 37.5 22.5 39.5 72-73 31.813147474389957 35.0 31.0 37.0 21.5 39.0 74-75 31.347911942354322 34.5 31.0 36.0 20.0 39.0 76-77 30.092495922212102 33.5 29.5 35.0 19.5 37.0 78-79 30.722261223115154 34.0 30.5 35.0 20.0 37.0 80-81 30.58566605072845 34.0 31.0 35.0 18.5 37.0 82-83 30.23773785591199 34.0 30.0 35.0 17.5 36.0 84-85 29.877423704848063 34.0 30.0 35.0 13.5 36.0 86-87 29.562642858013525 34.0 30.0 35.0 7.5 35.5 88-89 29.184390503325208 34.0 29.0 35.0 4.5 35.0 90-91 28.70506047496015 34.0 29.0 35.0 2.0 35.0 92-93 28.269342800091557 33.0 29.0 35.0 2.0 35.0 94-95 27.78753923728098 33.0 27.0 35.0 2.0 35.0 96-97 27.265670420834937 33.0 27.0 35.0 2.0 35.0 98-99 26.444103493873047 32.5 25.0 34.0 2.0 35.0 100-101 23.994320667398327 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25646626990616994 1101 2 0.25119323997452625 1101 3 0.33734933669682476 1101 4 0.1884954917907038 1101 5 0.1334915491564388 1101 6 0.18782337740673682 1101 7 0.18692372972225968 1101 8 0.2107726543583155 1101 9 0.2570231036139887 1101 10-11 0.3113752201211284 1101 12-13 0.26648085299203217 1101 14-15 0.39068169969098676 1101 16-17 0.39197472213741946 1101 18-19 0.3563491707104589 1101 20-21 0.3423673596737302 1101 22-23 0.3212500960772857 1101 24-25 0.2564648186855223 1101 26-27 0.25720828095904125 1101 28-29 0.40229357161669554 1101 30-31 0.5163392748155502 1101 32-33 0.5012538121701198 1101 34-35 0.4530066946426601 1101 36-37 0.3810333390632721 1101 38-39 0.4408878971442789 1101 40-41 0.5030213445045533 1101 42-43 0.45292984705733375 1101 44-45 0.5621843793745782 1101 46-47 0.5688296927729795 1101 48-49 0.6445593468457957 1101 50-51 0.6798383497614751 1101 52-53 0.7349891083229991 1101 54-55 0.6086838228530169 1101 56-57 0.6409427115171766 1101 58-59 0.719991550217884 1101 60-61 0.6273777270974463 1101 62-63 0.5941549548612102 1101 64-65 0.6610521327041425 1101 66-67 0.6242117778601894 1101 68-69 0.5995513487247734 1101 70-71 0.6067131941704744 1101 72-73 0.5449429136943067 1101 74-75 0.732761688080835 1101 76-77 0.6567396131046621 1101 78-79 0.6398623957605132 1101 80-81 0.8273524554167686 1101 82-83 0.753772236603325 1101 84-85 0.79958915008066 1101 86-87 0.8685544585115856 1101 88-89 0.9462750632145784 1101 90-91 0.769439329854464 1101 92-93 0.9324219091008068 1101 94-95 1.0284273078676271 1101 96-97 0.9824383817247728 1101 98-99 1.0861305439945994 1101 100-101 1.4714732448753445 1102 1 0.15341361459625347 1102 2 0.16742570408699464 1102 3 0.21231179356393426 1102 4 0.17237122606022837 1102 5 0.018248777646356018 1102 6 0.09841992202948546 1102 7 0.07192920638470213 1102 8 0.1301605956152727 1102 9 0.17454519751594688 1102 10-11 0.21530781230666918 1102 12-13 0.21925233341138295 1102 14-15 0.17075532501905144 1102 16-17 0.22632496452700934 1102 18-19 0.2198424322279493 1102 20-21 0.19837859925343082 1102 22-23 0.2566287236709144 1102 24-25 0.2125714270926622 1102 26-27 0.2187016336924401 1102 28-29 0.17293460068045619 1102 30-31 0.17520104860157915 1102 32-33 0.19067087577430897 1102 34-35 0.16837765045930553 1102 36-37 0.25996869368179887 1102 38-39 0.2595515062240423 1102 40-41 0.33441569089807643 1102 42-43 0.3230372815987934 1102 44-45 0.34520351840022556 1102 46-47 0.2699192408389379 1102 48-49 0.2269988755191079 1102 50-51 0.23471704743561617 1102 52-53 0.22280965251859897 1102 54-55 0.2636879238609069 1102 56-57 0.2744390469366067 1102 58-59 0.2524117604001006 1102 60-61 0.2875762218124862 1102 62-63 0.31021735321665744 1102 64-65 0.3924320365788887 1102 66-67 0.4016515438431796 1102 68-69 0.2879047829295516 1102 70-71 0.31630501039785486 1102 72-73 0.3100654730198755 1102 74-75 0.42519304811002456 1102 76-77 0.2988317500709101 1102 78-79 0.09917741249455503 1102 80-81 0.24188416925985834 1102 82-83 0.2825673560331303 1102 84-85 0.3347770770990337 1102 86-87 0.23558289266074794 1102 88-89 0.176612473083253 1102 90-91 0.35349525262202874 1102 92-93 0.545533621149243 1102 94-95 0.7284269926907072 1102 96-97 0.67339749475018 1102 98-99 0.6612891150640294 1102 100-101 1.1546216249772243 1103 1 0.22485629985828126 1103 2 0.2665133507766626 1103 3 0.22271013672684603 1103 4 0.13081661580286408 1103 5 0.1002202746178753 1103 6 0.20185897837507127 1103 7 0.22444485741046094 1103 8 0.19149045583807833 1103 9 0.28552949572090824 1103 10-11 0.25696055760124636 1103 12-13 0.25197138679212117 1103 14-15 0.22072203663118728 1103 16-17 0.2058479261403292 1103 18-19 0.2861986404791992 1103 20-21 0.335747797444796 1103 22-23 0.34828023045972145 1103 24-25 0.29586245269077693 1103 26-27 0.27654562016076767 1103 28-29 0.3218470508022335 1103 30-31 0.3053407766332725 1103 32-33 0.16716044367949934 1103 34-35 0.3306791988184159 1103 36-37 0.2977027499999636 1103 38-39 0.3369633165772328 1103 40-41 0.4427247221010546 1103 42-43 0.4494732155862522 1103 44-45 0.5082323816353451 1103 46-47 0.32805101444916573 1103 48-49 0.3386143389537821 1103 50-51 0.3843986947277074 1103 52-53 0.28487486372353743 1103 54-55 0.3068719728142213 1103 56-57 0.39998788934970264 1103 58-59 0.25520615085766707 1103 60-61 0.3928816401712325 1103 62-63 0.19911629511674533 1103 64-65 0.168365165587133 1103 66-67 0.25594181291281615 1103 68-69 0.279802024389582 1103 70-71 0.32823446813464585 1103 72-73 0.18450263832786717 1103 74-75 0.27871771262150347 1103 76-77 0.34794720168778426 1103 78-79 0.36340051673457197 1103 80-81 0.39184225860283206 1103 82-83 0.4895176045946208 1103 84-85 0.31599187540884444 1103 86-87 0.42809481115156345 1103 88-89 0.5460063672687241 1103 90-91 0.6064996690768716 1103 92-93 0.6646374119034064 1103 94-95 0.889163485745339 1103 96-97 0.8955806079225681 1103 98-99 1.167361575025783 1103 100-101 1.6998960069486664 1104 1 0.16655718190509106 1104 2 0.13141130902138087 1104 3 0.15221435895320212 1104 4 0.12715224322053587 1104 5 0.1604977606014728 1104 6 0.2103684704635853 1104 7 0.10817851051722016 1104 8 0.11529024415688127 1104 9 0.18739533099897443 1104 10-11 0.19253550460252455 1104 12-13 0.22943179381311296 1104 14-15 0.3491856707343146 1104 16-17 0.30384343779628153 1104 18-19 0.2322352018078675 1104 20-21 0.17897551534289846 1104 22-23 0.18024986313783842 1104 24-25 0.07970807389938983 1104 26-27 0.12554479807147345 1104 28-29 0.2703080540523217 1104 30-31 0.3315522708678742 1104 32-33 0.25528555098924244 1104 34-35 0.311383000108286 1104 36-37 0.1952780780357628 1104 38-39 0.16034566087642332 1104 40-41 0.34150275569106014 1104 42-43 0.3777301876082433 1104 44-45 0.2971246189249044 1104 46-47 0.35815483276220306 1104 48-49 0.2843927295283848 1104 50-51 0.31076764279846714 1104 52-53 0.35308171569455027 1104 54-55 0.36503296052595857 1104 56-57 0.34363242387797754 1104 58-59 0.23734102745748942 1104 60-61 0.2759594621846091 1104 62-63 0.22640888591934072 1104 64-65 0.4010966372608138 1104 66-67 0.35096140733063663 1104 68-69 0.21807077251133222 1104 70-71 0.2680847604667562 1104 72-73 0.13694599604085766 1104 74-75 0.321573848667974 1104 76-77 0.1429576362198013 1104 78-79 0.2399204460737927 1104 80-81 0.31531988244480047 1104 82-83 0.2101364866326456 1104 84-85 0.06872494065169477 1104 86-87 0.23984608720939704 1104 88-89 0.22273747589304804 1104 90-91 0.05875596017218854 1104 92-93 0.28295793638424627 1104 94-95 0.2084237768665851 1104 96-97 0.47971415905763237 1104 98-99 0.6006810569945067 1104 100-101 1.0148506289162036 1105 1 0.18315658283339076 1105 2 0.21664870857595275 1105 3 0.17219958302698757 1105 4 0.1670523881382735 1105 5 0.15628740549912834 1105 6 0.18209211949026383 1105 7 0.15040748054305908 1105 8 0.162578597140822 1105 9 0.28696038181816164 1105 10-11 0.2700848662883928 1105 12-13 0.30820582796430784 1105 14-15 0.28843718874517776 1105 16-17 0.38733293248508005 1105 18-19 0.4232373192333725 1105 20-21 0.48324605686883615 1105 22-23 0.3347089985893845 1105 24-25 0.3415231808141854 1105 26-27 0.2038198507621516 1105 28-29 0.28843942849761106 1105 30-31 0.370300741098994 1105 32-33 0.40171346102853533 1105 34-35 0.3830676761598255 1105 36-37 0.3801936994624242 1105 38-39 0.41272274290535194 1105 40-41 0.40592366284278825 1105 42-43 0.4389753239475098 1105 44-45 0.5433310615422826 1105 46-47 0.5062038813904834 1105 48-49 0.5793311909311782 1105 50-51 0.499471922427972 1105 52-53 0.5230635348383927 1105 54-55 0.5919874638030223 1105 56-57 0.5904531011601861 1105 58-59 0.6697750918464251 1105 60-61 0.7230827373058872 1105 62-63 0.4814145003793726 1105 64-65 0.6575217890618958 1105 66-67 0.7172875320108432 1105 68-69 0.8018533606304317 1105 70-71 0.5793011141248243 1105 72-73 0.6373364606692746 1105 74-75 0.8073985618944874 1105 76-77 0.7832376880335268 1105 78-79 0.7209804620059259 1105 80-81 0.8140110665667528 1105 82-83 0.722830261440869 1105 84-85 0.7601525518410313 1105 86-87 0.9039601587315467 1105 88-89 1.096392038646151 1105 90-91 1.1117512452015674 1105 92-93 1.0045622903833014 1105 94-95 1.1819019446517345 1105 96-97 1.062272319670381 1105 98-99 1.2243696690470784 1105 100-101 1.533742478542738 1106 1 -0.29310010810807796 1106 2 -0.1979106145201115 1106 3 -0.20688164000675613 1106 4 -0.15030397610433965 1106 5 -0.1577596992017689 1106 6 -0.15756974778935984 1106 7 -0.14072340828355578 1106 8 -0.10131615451247455 1106 9 -0.41527779500596296 1106 10-11 -0.38504831404156903 1106 12-13 -0.4756608001559144 1106 14-15 -0.42079270414064496 1106 16-17 -0.2677708354889674 1106 18-19 -0.2763129525427104 1106 20-21 -0.22639206178090632 1106 22-23 -0.3289848031187219 1106 24-25 -0.3445331750333409 1106 26-27 -0.33307610221591943 1106 28-29 -0.29533464677200527 1106 30-31 -0.49413357209441244 1106 32-33 -0.5323801004146134 1106 34-35 -0.7879947336077961 1106 36-37 -0.5659932176851825 1106 38-39 -0.5237291278387275 1106 40-41 -0.5249036490866317 1106 42-43 -0.7164153253472421 1106 44-45 -0.7126745276921511 1106 46-47 -0.5467215099564342 1106 48-49 -0.671836730859745 1106 50-51 -0.894170485991701 1106 52-53 -0.7038516085933963 1106 54-55 -0.6192890265313977 1106 56-57 -0.7813161215141093 1106 58-59 -0.8335183530114634 1106 60-61 -0.9169678164740844 1106 62-63 -1.0720824365971993 1106 64-65 -1.3965465457435329 1106 66-67 -1.4031881382065876 1106 68-69 -1.278082328292669 1106 70-71 -1.2655874322603786 1106 72-73 -1.2505418649961584 1106 74-75 -1.0943446596659854 1106 76-77 -1.1191542311655596 1106 78-79 -1.1876547100873935 1106 80-81 -1.3232051115887629 1106 82-83 -1.5228453832365894 1106 84-85 -1.1918825467066192 1106 86-87 -0.9394019011620607 1106 88-89 -1.0669248710641241 1106 90-91 -1.2837490190662315 1106 92-93 -1.1232218329747816 1106 94-95 -0.9857397637834318 1106 96-97 -1.1090895344845357 1106 98-99 -1.0552682308575037 1106 100-101 -0.57110133741336 1107 1 0.1608517740842288 1107 2 0.17940687653030096 1107 3 0.09869094850890292 1107 4 0.11328474493785734 1107 5 0.08563832052274734 1107 6 0.07112542736001615 1107 7 0.013307041215682602 1107 8 0.059147567455873684 1107 9 0.1690371357164011 1107 10-11 0.10780046063741366 1107 12-13 0.1244453887014032 1107 14-15 0.16082260531111103 1107 16-17 0.18330745708429674 1107 18-19 0.3533105082300949 1107 20-21 0.19908872953502055 1107 22-23 0.2689922739581547 1107 24-25 0.13310966942918157 1107 26-27 0.08670389154256242 1107 28-29 0.13485158827138832 1107 30-31 0.16070255535236555 1107 32-33 0.25805131441632057 1107 34-35 0.24935526314696688 1107 36-37 0.29297609542435055 1107 38-39 0.3462147132816469 1107 40-41 0.2180772318807911 1107 42-43 0.27761625627992004 1107 44-45 0.41282066713610277 1107 46-47 0.19698977134267892 1107 48-49 0.25203552006248486 1107 50-51 0.37990227308407043 1107 52-53 0.3921666204443781 1107 54-55 0.49640125883300357 1107 56-57 0.459013742627576 1107 58-59 0.39346366364029706 1107 60-61 0.49034572991070746 1107 62-63 0.45730991596759196 1107 64-65 0.5323641189904222 1107 66-67 0.48187805719454246 1107 68-69 0.5514359512759484 1107 70-71 0.6273648343219591 1107 72-73 0.6926026410567232 1107 74-75 0.7080707145126723 1107 76-77 0.48622420630230323 1107 78-79 0.6766407907117618 1107 80-81 0.6714947811554559 1107 82-83 0.7522138386121782 1107 84-85 0.7251438867021811 1107 86-87 0.8822703169817991 1107 88-89 0.7926641487175878 1107 90-91 0.8974095271511118 1107 92-93 0.911801522350089 1107 94-95 1.020381580769623 1107 96-97 0.9655205111651135 1107 98-99 1.0530352161756547 1107 100-101 1.6212546059766844 1108 1 0.1664414903871574 1108 2 0.10396968076447166 1108 3 0.09218445407012155 1108 4 0.1472858156996466 1108 5 0.12805615983066332 1108 6 0.1012423837004448 1108 7 0.08342577302602194 1108 8 0.04865667742486579 1108 9 0.09170720134851251 1108 10-11 0.1053606964680327 1108 12-13 0.15777901429315477 1108 14-15 0.1789645924893719 1108 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-0.5534017779594507 2316 58-59 -0.49738802614988487 2316 60-61 -0.5185960133687999 2316 62-63 -0.513114464987865 2316 64-65 -0.629669363833024 2316 66-67 -0.529830834875753 2316 68-69 -0.41568174341287545 2316 70-71 -0.37519594946122226 2316 72-73 -0.11244401318496244 2316 74-75 -0.21923660579126647 2316 76-77 -0.2481555578115895 2316 78-79 -0.20510166091260373 2316 80-81 -0.3781113686025428 2316 82-83 -0.4459583570347725 2316 84-85 -0.38855848730309717 2316 86-87 -0.2624557647686281 2316 88-89 -0.1976573930026717 2316 90-91 -0.3093552874131724 2316 92-93 -0.06255833218478912 2316 94-95 -0.23993969845111707 2316 96-97 0.05275394795018329 2316 98-99 -0.19702869236143528 2316 100-101 0.1419771258105449 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 17.0 5 41.0 6 84.0 7 270.0 8 595.0 9 1159.0 10 1918.0 11 3020.0 12 4777.0 13 6587.0 14 8683.0 15 11371.0 16 15087.0 17 20353.0 18 19219.0 19 13103.0 20 11697.0 21 11939.0 22 12371.0 23 13418.0 24 15028.0 25 17927.0 26 21930.0 27 27421.0 28 34190.0 29 42925.0 30 53949.0 31 67739.0 32 87528.0 33 115293.0 34 159341.0 35 232403.0 36 356611.0 37 499915.0 38 309653.0 39 17479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89018254735302 18.60077137773121 14.912655472355954 23.59639060255982 2 16.43259359048039 19.72335600702287 38.45355114040366 25.390499262093076 3 19.518484692663684 22.42921036961259 30.02213495051351 28.03016998721021 4 14.089284867801782 16.070282996507974 34.78290508770702 35.05752704798322 5 14.85869214324253 36.187859647063334 33.54948088918186 15.40396732051227 6 34.725416909513065 34.33296509467736 16.009803846325745 14.931814149483827 7 29.98254214692316 32.12556471662704 19.39370190004826 18.498191236401542 8 27.357137471902426 34.307954361280494 18.862291533957038 19.47261663286004 9 26.59236883698735 16.061360267876694 19.64137136469667 37.704899530439285 10-11 26.022200863276613 25.677874394652218 26.252421503993816 22.04750323807735 12-13 26.28578123433915 22.739645440598704 27.370336057587686 23.604237267474463 14-15 23.2493050943794 24.447134126603974 25.194995686325676 27.108565092690952 16-17 23.25330049132096 27.645347800432408 26.042900179634852 23.05845152861178 18-19 23.156462904432363 25.914866894592397 28.332510928652145 22.596159272323096 20-21 23.50638339752321 25.128857543623305 28.65540307795379 22.709355980899694 22-23 23.46652280007133 24.820621982243914 28.703078760188983 23.00977645749577 24-25 23.47571092106766 24.98527120619002 28.633136332424247 22.90588154031807 26-27 23.172176386271033 25.465446213925354 28.478143587544157 22.884233812259456 28-29 23.07693175892714 25.684656763046753 28.48192901628206 22.756482461744053 30-31 23.2280745157667 25.22985957866376 28.479947161415915 23.062118744153626 32-33 23.304360309862222 25.10614889332019 28.475013419567695 23.114477377249894 34-35 23.70789540095953 25.164815767055266 28.185516437785353 22.94177239419986 36-37 23.780310111711277 25.21218009332109 28.056913632053227 22.9505961629144 38-39 23.37442647995597 25.27844756481685 28.216373542978058 23.13075241224912 40-41 23.507733814045807 25.2105656520374 28.1405506117143 23.1411499222025 42-43 23.542837403678778 25.275321221261677 27.84938103806791 23.33246033699164 44-45 23.460541956408186 25.399957292141167 27.838187778238765 23.30131297321188 46-47 23.745253491006295 25.736225248290218 27.311766015108468 23.20675524559502 48-49 23.988160081334545 25.625347339945982 27.062606741431 23.323885837288476 50-51 23.782182696635605 25.939381024979586 26.992540571337248 23.285895707047562 52-53 24.08538684367408 25.88358674764909 26.680747622456124 23.350278786220706 54-55 23.77922565144157 25.86299445294438 26.72672252397125 23.631057371642793 56-57 23.697760184989136 26.171994389288905 26.87130171838185 23.25894370734011 58-59 23.954007337986656 25.793966634598153 26.773267769697195 23.478758257718003 60-61 23.6922975518826 25.84581650338209 27.254746960450987 23.20713898428432 62-63 23.50670346344483 25.899697929932923 27.311788587972547 23.2818100186497 64-65 23.590448789168995 25.93263173862068 27.021817576044594 23.45510189616573 66-67 23.561420085966496 25.91504747750501 26.946785375853988 23.576747060674506 68-69 23.45510189616573 25.69945405270944 27.359575341222698 23.485868709902128 70-71 23.389866318984364 25.93288004012552 27.049243605897654 23.628010034992453 72-73 23.566860146208953 25.938997286290284 26.69810013232213 23.796042435178634 74-75 23.48081238834897 26.318401985689704 26.482168114565415 23.71861751139591 76-77 23.717737169696925 26.0131491447819 26.747196111688726 23.52191757383245 78-79 23.711552204939938 26.022110571820306 26.706903549312 23.559433673927753 80-81 23.571239281839816 26.20571824821934 26.687581177662434 23.53546129227841 82-83 23.651453031466904 26.18029955646429 26.63679213233702 23.53145527973178 84-85 23.551664685516265 26.042880431666564 26.4745876265156 23.93086725630157 86-87 23.61180271858546 25.87048864381781 26.73776065450471 23.779947983092022 88-89 23.6230440048956 26.084930852545472 26.510068174564083 23.781956967994837 90-91 23.611857343452996 26.006239756550166 26.562964200412676 23.818938699584166 92-93 23.29151395800094 25.682640467141795 26.838982538164753 24.186863036692518 94-95 23.359641253985803 25.74746653460036 26.82405671386954 24.068835497544296 96-97 23.543384367569296 25.602864767325688 26.722275669748164 24.131475195356852 98-99 23.214196228599135 25.782751454940524 26.50772740344527 24.495324913015075 100-101 23.206784108826042 25.621556066470287 26.83586151842815 24.33579830627552 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 300.0 1 244.0 2 248.0 3 362.0 4 316.5 5 295.0 6 611.0 7 1390.0 8 2225.0 9 2587.0 10 2435.5 11 2162.5 12 2243.5 13 2497.0 14 2731.5 15 2914.0 16 3111.0 17 3320.5 18 3416.5 19 3565.5 20 3934.5 21 4398.0 22 5037.5 23 5831.5 24 6762.0 25 8258.0 26 9860.0 27 12037.0 28 14354.5 29 16417.5 30 18664.5 31 21379.0 32 23990.0 33 25063.5 34 26296.5 35 28151.0 36 31034.5 37 35630.5 38 41107.0 39 45013.5 40 48332.5 41 54547.5 42 60068.5 43 64427.0 44 70311.5 45 76451.5 46 81141.0 47 85455.0 48 89544.0 49 91221.5 50 91620.5 51 90221.5 52 90674.5 53 93456.5 54 95474.5 55 91059.0 56 81440.5 57 74508.5 58 69506.0 59 62072.0 60 51630.0 61 42109.0 62 34194.5 63 27000.5 64 20545.0 65 15508.5 66 11374.5 67 9101.0 68 6640.0 69 4091.0 70 3011.5 71 2176.5 72 1628.0 73 1296.0 74 1006.0 75 801.0 76 523.5 77 303.5 78 202.0 79 129.5 80 85.5 81 47.0 82 29.5 83 17.5 84 7.0 85 4.0 86 2.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03742580863899625 2 0.0 3 0.0 4 1.8058291261276838E-4 5 0.009209728543251187 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005914090388068165 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.708743689191526E-4 22-23 0.0015349547572085312 24-25 0.0 26-27 0.0010834974756766104 28-29 0.0015349547572085312 30-31 4.5145728153192096E-5 32-33 0.0 34-35 0.0 36-37 3.837386893021328E-4 38-39 0.0 40-41 1.5801004853617233E-4 42-43 0.02049616058154921 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2572864076596048E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.3859296114894074E-4 84-85 2.708743689191526E-4 86-87 0.0 88-89 0.0 90-91 0.009886914465549068 92-93 0.0021218492232000286 94-95 0.0 96-97 0.0 98-99 4.51457281531921E-4 100-101 0.012866532523659747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2215049.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.827112232994864 #Duplication Level Percentage of deduplicated Percentage of total 1 63.07859605790432 23.860811325820944 2 16.144783552272873 12.21421078818471 3 7.193903956658803 8.16373837085755 4 3.8417779680100033 5.812934654806454 5 2.411988727246304 4.561928414513219 6 1.5465445187242977 3.510078784986422 7 1.0893032207257336 2.884363663030857 8 0.7866838498898564 2.380638262533447 9 0.6094171904691156 2.0747243214532483 >10 2.993129559322185 20.087100313713403 >50 0.1974151350244007 5.140311122257487 >100 0.09999235178895423 6.588249253785669 >500 0.00562599744941961 1.4292580753331845 >1k 4.788082935676246E-4 0.2985752494454932 >5k 3.5910622017571846E-4 0.9930773992779128 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9207 0.4156567191064396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7571 0.34179830784781734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5154 0.23268108290155207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.029145630638419E-5 0.0 22-23 0.0 0.0 0.0 1.5801004853617235E-4 0.0 24-25 0.0 0.0 0.0 2.9344723299574863E-4 0.0 26-27 0.0 0.0 0.0 4.96603009685113E-4 0.0 28-29 0.0 0.0 0.0 0.0015801004853617233 0.0 30-31 0.0 0.0 0.0 0.0045371456793958055 0.0 32-33 0.0 0.0 0.0 0.009006572766561824 0.0 34-35 0.0 0.0 0.0 0.01302454257219592 0.0 36-37 0.0 0.0 0.0 0.017177949562289595 0.0 38-39 0.0 0.0 0.0 0.022595436940672644 0.0 40-41 0.0 0.0 0.0 0.02923185897919188 0.0 42-43 0.0 0.0 0.0 0.038802753347668605 0.0 44-45 0.0 0.0 0.0 0.04751587888123468 0.0 46-47 0.0 0.0 0.0 0.05765109485162631 0.0 48-49 0.0 0.0 0.0 0.06826034096762645 0.0 50-51 0.0 0.0 0.0 0.083316441306716 0.0 52-53 0.0 0.0 0.0 0.12026821980010374 0.0 54-55 0.0 0.0 0.0 0.17387877198201937 0.0 56-57 0.0 0.0 0.0 0.2194082388245136 0.0 58-59 0.0 0.0 0.0 0.28051298187985907 0.0 60-61 0.0 0.0 0.0 0.3523849810997409 0.0 62-63 0.0 0.0 0.0 0.40281275944685646 0.0 64-65 0.0 0.0 0.0 0.4478004775515124 0.0 66-67 0.0 0.0 0.0 0.5664660240021778 0.0 68-69 0.0 0.0 0.0 0.683190304142256 0.0 70-71 0.0 0.0 0.0 0.7456268461781206 0.0 72-73 0.0 0.0 0.0 0.8644504026773223 0.0 74-75 0.0 0.0 0.0 1.0346723706789331 0.0 76-77 0.0 0.0 0.0 1.2078739567386547 0.0 78-79 0.0 0.0 0.0 1.3812561257109888 0.0 80-81 0.0 0.0 0.0 1.599513148467596 0.0 82-83 0.0 0.0 0.0 1.8396432765144248 0.0 84-85 0.0 0.0 0.0 2.091488721017007 0.0 86-87 0.0 0.0 0.0 2.4408263654664073 0.0 88-89 0.0 0.0 0.0 2.8372961501077403 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 48.070942 1 GTATCAA 7760 0.0 39.133434 1 TATCAAC 10000 0.0 30.022675 2 ATCAACG 9990 0.0 30.005177 3 TCAACGC 10195 0.0 29.44843 4 CAACGCA 10300 0.0 29.194347 5 AACGCAG 10600 0.0 28.40906 6 ACGCAGA 12060 0.0 24.735188 7 CGCAGAG 12155 0.0 24.30739 8 GAGTACT 8235 0.0 22.120995 12-13 GCAGAGT 13415 0.0 21.81187 9 GTACATG 6280 0.0 21.037493 1 AGAGTAC 11555 0.0 20.78041 10-11 TAAACCG 115 0.00372243 20.654015 5 AGTACTT 8585 0.0 20.25087 12-13 CAGAGTA 12490 0.0 20.13754 10-11 GTACTTT 9035 0.0 20.055794 14-15 TACATGG 6520 0.0 19.963434 2 ACATGGG 6465 0.0 18.663689 3 AATTCGC 565 0.0 17.654846 78-79 >>END_MODULE