FastQCFastQC Report
Thu 26 May 2016
SRR937572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129534
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.31617245838760966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53020.248974658305526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.162570778395649No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29840.14012455307123528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.042.261191
GTATCAA70450.039.693121
ATCAACG86250.032.2985843
TCAACGC87950.031.7298244
TATCAAC88000.031.7110522
AACGTAC600.0039283731.7049921
CAACGCA89300.031.1422045
AACGCAG91400.030.4044846
ACGCAGA104650.026.1009657
CGCAGAG105600.025.6862148
GTACATG62350.024.5605621
TACATGG63300.023.281622
GAGTACT65750.023.2256112-13
GCAGAGT114950.023.0993679
GTATAGG3301.8189894E-1221.6170391
AGAGTAC97300.021.25995610-11
AGTACTT69000.021.13349212-13
ACATGGG65400.021.007053
GTACTTT72400.020.95987514-15
CAGAGTA106700.020.43314710-11
Produced by FastQC (version 0.11.5)