##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937572_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2129534 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76175773666915 33.0 31.0 34.0 30.0 34.0 2 31.871032347922128 33.0 31.0 34.0 30.0 34.0 3 31.927473804128038 33.0 31.0 34.0 30.0 34.0 4 35.55138072460923 37.0 35.0 37.0 33.0 37.0 5 35.544246299894716 37.0 35.0 37.0 33.0 37.0 6 35.60072907969537 37.0 35.0 37.0 33.0 37.0 7 35.52219969251489 37.0 35.0 37.0 33.0 37.0 8 35.52010627677229 37.0 35.0 37.0 33.0 37.0 9 37.09029909830038 39.0 37.0 39.0 33.0 39.0 10-11 37.01535030668681 39.0 37.0 39.0 33.0 39.0 12-13 36.909228967464244 39.0 37.0 39.0 33.0 39.0 14-15 38.054109960207256 40.0 38.0 41.0 33.0 41.0 16-17 37.94719807244214 40.0 38.0 41.0 33.0 41.0 18-19 37.83794482736599 40.0 38.0 41.0 33.0 41.0 20-21 37.82840189449898 40.0 37.5 41.0 33.0 41.0 22-23 37.833290287922146 40.0 38.0 41.0 33.0 41.0 24-25 37.74971120442312 40.0 37.5 41.0 33.0 41.0 26-27 37.64296672417534 40.0 37.0 41.0 32.0 41.0 28-29 37.491329793278716 40.0 37.0 41.0 32.0 41.0 30-31 37.26205075852276 39.5 37.0 41.0 31.5 41.0 32-33 37.13971460422797 39.0 36.5 41.0 31.0 41.0 34-35 37.13079856907662 40.0 36.0 41.0 31.0 41.0 36-37 36.97135969653455 39.0 36.0 41.0 31.0 41.0 38-39 36.80192168803128 39.0 36.0 41.0 30.5 41.0 40-41 36.56402480542691 39.0 35.5 41.0 30.0 41.0 42-43 36.29989542313013 39.0 35.0 40.5 30.0 41.0 44-45 36.0505969850681 39.0 35.0 40.0 29.5 41.0 46-47 35.69546365542884 38.5 35.0 40.0 28.5 41.0 48-49 35.32899310365554 38.0 35.0 40.0 27.0 41.0 50-51 34.42079112143783 37.5 33.5 39.5 25.0 40.5 52-53 34.50976293405036 38.0 34.0 39.5 26.0 40.5 54-55 34.75204903983688 38.0 34.0 40.0 26.0 41.0 56-57 34.46994600696678 38.0 34.0 40.0 25.5 41.0 58-59 34.14259457702953 37.5 33.5 40.0 24.5 41.0 60-61 33.75585104534608 37.0 33.0 40.0 23.0 41.0 62-63 33.34366650168535 36.5 33.0 39.0 22.5 41.0 64-65 32.86704720375444 36.0 32.0 39.0 21.5 40.5 66-67 32.34113895340483 35.0 31.5 38.5 19.5 40.0 68-69 31.83352320272886 35.0 31.0 38.0 18.0 40.0 70-71 31.34625016552917 35.0 31.0 37.0 16.0 39.5 72-73 30.85653715789464 34.5 30.0 37.0 11.5 39.0 74-75 30.294891276683067 34.0 29.5 36.0 7.5 39.0 76-77 29.74612192150959 34.0 29.0 35.5 4.5 38.0 78-79 29.22108968440983 34.0 29.0 35.0 2.0 37.0 80-81 28.685426952563333 34.0 28.0 35.0 2.0 36.5 82-83 28.143562159608628 33.0 27.0 35.0 2.0 36.0 84-85 27.604300753122516 33.0 26.0 35.0 2.0 36.0 86-87 27.010456278228006 32.5 25.5 35.0 2.0 35.0 88-89 26.45064600987822 32.0 24.5 34.5 2.0 35.0 90-91 25.858113793909844 32.0 23.5 34.0 2.0 35.0 92-93 25.144557447779654 31.0 19.5 34.0 2.0 35.0 94-95 24.324392801429795 31.0 15.5 34.0 2.0 35.0 96-97 23.3627096350657 31.0 3.5 34.0 2.0 35.0 98-99 22.090361553278793 30.0 2.0 34.0 2.0 35.0 100-101 18.872528449886218 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11072382996109198 1101 2 0.12596963122116378 1101 3 0.11175423005807872 1101 4 0.17343874306208562 1101 5 0.06705806606004927 1101 6 0.10060823034724109 1101 7 0.1380612606193239 1101 8 0.2200373919671108 1101 9 0.457551953974523 1101 10-11 0.39410907062750056 1101 12-13 0.40065086684615636 1101 14-15 0.5466734996145206 1101 16-17 0.6088642238613744 1101 18-19 0.7436706692180479 1101 20-21 0.6450424268587156 1101 22-23 0.5979700221950779 1101 24-25 0.5330998768094943 1101 26-27 0.6522643228896499 1101 28-29 0.5618594164050847 1101 30-31 0.603722901913514 1101 32-33 0.643654578450537 1101 34-35 0.708264546072968 1101 36-37 0.7021480636978623 1101 38-39 0.5742960625606415 1101 40-41 0.7103380396405754 1101 42-43 0.6546430430401458 1101 44-45 0.8438308288891818 1101 46-47 0.9124447871552803 1101 48-49 1.0264270337665238 1101 50-51 0.833191229429417 1101 52-53 0.9667517061075728 1101 54-55 1.039459171436043 1101 56-57 1.0837485904806314 1101 58-59 1.0348778260016047 1101 60-61 1.1624720601780822 1101 62-63 1.1122196711619239 1101 64-65 1.1263723000840073 1101 66-67 1.0717035517347284 1101 68-69 1.206759565227923 1101 70-71 1.1478512926520743 1101 72-73 1.239821497426167 1101 74-75 1.3746318777661841 1101 76-77 1.4413258230629857 1101 78-79 1.566845348895228 1101 80-81 1.7651226875166017 1101 82-83 1.9154058535104426 1101 84-85 2.198346078257263 1101 86-87 2.339763494838504 1101 88-89 2.533105404243006 1101 90-91 2.509853558185661 1101 92-93 2.5414168751368216 1101 94-95 2.850061931101987 1101 96-97 3.077921833728233 1101 98-99 3.1582661935547485 1101 100-101 3.758482412344481 1102 1 0.29066043036521805 1102 2 0.26440916302676953 1102 3 0.19325258490495756 1102 4 0.2523469671680374 1102 5 0.20384310906638348 1102 6 0.07296696086161347 1102 7 0.22747108008814365 1102 8 0.19010763772352846 1102 9 0.36713595636445717 1102 10-11 0.3854983640622365 1102 12-13 0.38184031463069346 1102 14-15 0.47081077965567175 1102 16-17 0.5123067015906102 1102 18-19 0.4722395934580206 1102 20-21 0.5198356935560824 1102 22-23 0.43384099903845197 1102 24-25 0.5181740020568881 1102 26-27 0.484100629116341 1102 28-29 0.49808691224552604 1102 30-31 0.47150144729111076 1102 32-33 0.5670288227796405 1102 34-35 0.5409948986294921 1102 36-37 0.5183362252340586 1102 38-39 0.5994089204799877 1102 40-41 0.6189213615636362 1102 42-43 0.6196104096748627 1102 44-45 0.8329575699922813 1102 46-47 0.6954153777092387 1102 48-49 0.7935789479945043 1102 50-51 0.6766647638324059 1102 52-53 0.8447306157838952 1102 54-55 0.8779007778357553 1102 56-57 0.8867842403057367 1102 58-59 0.764770756199816 1102 60-61 0.8239516192023615 1102 62-63 0.8444909160428651 1102 64-65 0.7438782437961251 1102 66-67 0.86364748790362 1102 68-69 0.9788384329974384 1102 70-71 1.021268584808869 1102 72-73 1.0918026310673739 1102 74-75 0.9836396616432665 1102 76-77 1.1111105009407574 1102 78-79 1.2887264041167725 1102 80-81 1.5415841353343396 1102 82-83 1.7230094483671081 1102 84-85 1.8787898131905223 1102 86-87 1.949832018681814 1102 88-89 1.993749420491227 1102 90-91 2.032490699229019 1102 92-93 2.1244644331321005 1102 94-95 2.246881059689482 1102 96-97 2.130358444505333 1102 98-99 2.417314950665425 1102 100-101 3.1478130860481706 1103 1 0.14578403171833187 1103 2 0.11918147632367138 1103 3 0.2130938547462229 1103 4 0.09113697497443951 1103 5 -0.003463682502697907 1103 6 0.08418996372395782 1103 7 0.11250100462626023 1103 8 0.11191612067955248 1103 9 0.2173545355994264 1103 10-11 0.29975935807733123 1103 12-13 0.27648772550761436 1103 14-15 0.3571404700017595 1103 16-17 0.3654500791602189 1103 18-19 0.3462663954356415 1103 20-21 0.3355799038084939 1103 22-23 0.17587326549695348 1103 24-25 0.185878920089678 1103 26-27 0.3405070824080383 1103 28-29 0.3036307581992119 1103 30-31 0.4204085511162461 1103 32-33 0.3142094108618636 1103 34-35 0.2753092363864482 1103 36-37 0.4375412733735331 1103 38-39 0.4059415772585524 1103 40-41 0.5462177445977261 1103 42-43 0.2939325538330664 1103 44-45 0.49305905320851906 1103 46-47 0.37348069126635863 1103 48-49 0.4015544879372541 1103 50-51 0.48083468316626465 1103 52-53 0.5355802124531621 1103 54-55 0.5207982537660101 1103 56-57 0.5167022730926263 1103 58-59 0.5599906365016594 1103 60-61 0.7101551060615847 1103 62-63 0.7851115249421596 1103 64-65 0.5884723109311736 1103 66-67 0.9260635690901964 1103 68-69 0.9576674728100834 1103 70-71 1.0146058213428226 1103 72-73 0.9899147830319848 1103 74-75 1.1202490813674402 1103 76-77 1.0420498712243926 1103 78-79 1.223356901124319 1103 80-81 1.4080335238329056 1103 82-83 1.241233492082955 1103 84-85 1.5725498964588027 1103 86-87 1.5078609023664349 1103 88-89 1.6311746091459263 1103 90-91 1.848634337012001 1103 92-93 1.9626637565773244 1103 94-95 1.9189159282817236 1103 96-97 2.004671481195423 1103 98-99 2.4260034752555875 1103 100-101 3.0710228180283963 1104 1 0.2341572769701905 1104 2 0.1492970832600875 1104 3 0.22327827533527156 1104 4 0.15772932289626596 1104 5 0.15992194920663394 1104 6 0.1373746595676124 1104 7 0.33896123033504466 1104 8 0.2592296356809314 1104 9 0.40353950669769034 1104 10-11 0.3197758733539544 1104 12-13 0.3821772383069515 1104 14-15 0.5499246703871137 1104 16-17 0.5227842382573868 1104 18-19 0.49340787630544725 1104 20-21 0.5629250730900779 1104 22-23 0.41003786775403483 1104 24-25 0.38280570236327804 1104 26-27 0.47630532077852195 1104 28-29 0.44157322998561455 1104 30-31 0.5544162582453467 1104 32-33 0.5261926203306189 1104 34-35 0.5796871609666852 1104 36-37 0.5431619725202452 1104 38-39 0.6426522846628941 1104 40-41 0.7432725202652151 1104 42-43 0.76850040409456 1104 44-45 0.9073874319588597 1104 46-47 0.7440890039555939 1104 48-49 0.8897667092419681 1104 50-51 0.7797818342810459 1104 52-53 0.8988516134991329 1104 54-55 0.9751940698959345 1104 56-57 1.033417109316133 1104 58-59 1.072912464192278 1104 60-61 1.016285026237746 1104 62-63 1.126164373827379 1104 64-65 1.1947137092245939 1104 66-67 1.2542868005793153 1104 68-69 1.2449396996198878 1104 70-71 1.1693346955514663 1104 72-73 1.254938179673875 1104 74-75 1.3913225743484468 1104 76-77 1.3934880765643314 1104 78-79 1.593172480535273 1104 80-81 1.7258590124098419 1104 82-83 1.8119849371696617 1104 84-85 1.7851579338934265 1104 86-87 1.9158487911635724 1104 88-89 1.969841000557981 1104 90-91 2.1331058481598113 1104 92-93 2.2915035583940018 1104 94-95 2.671178521180156 1104 96-97 2.5815611040966076 1104 98-99 3.0726894097449886 1104 100-101 3.542948224028944 1105 1 0.25582732176161826 1105 2 0.2457832079254061 1105 3 0.17467394132630787 1105 4 0.1458339446714092 1105 5 0.11800817896916271 1105 6 0.25698217151617087 1105 7 0.16438412150937864 1105 8 0.18964339340682557 1105 9 0.3910341892790825 1105 10-11 0.25691303773100316 1105 12-13 0.23553534455523106 1105 14-15 0.3073893877506748 1105 16-17 0.34316652937667413 1105 18-19 0.46971877638802795 1105 20-21 0.37422708995567433 1105 22-23 0.2702022698259512 1105 24-25 0.3356949374056981 1105 26-27 0.3166326235335788 1105 28-29 0.3916285936970496 1105 30-31 0.357150975358671 1105 32-33 0.5674886316410834 1105 34-35 0.5145300156072352 1105 36-37 0.58111270194496 1105 38-39 0.5432900621070331 1105 40-41 0.6094753203553012 1105 42-43 0.7205451079456182 1105 44-45 0.6520309146803811 1105 46-47 0.6868032020888677 1105 48-49 0.4826150939978646 1105 50-51 0.6035078433394219 1105 52-53 0.587171121544074 1105 54-55 0.7577679507357118 1105 56-57 0.7518754332657878 1105 58-59 0.6684430174540452 1105 60-61 0.851940820347302 1105 62-63 0.9569297067603415 1105 64-65 0.9324117048705673 1105 66-67 1.014191274717895 1105 68-69 0.9200376026802211 1105 70-71 0.9111317953687994 1105 72-73 1.0580857787029814 1105 74-75 1.1315369601553193 1105 76-77 1.2692684859430052 1105 78-79 1.4269499747173917 1105 80-81 1.6252737835731672 1105 82-83 1.6304326262820865 1105 84-85 1.7357966497055557 1105 86-87 1.8489431534486833 1105 88-89 1.960449500920813 1105 90-91 2.11441355779122 1105 92-93 2.300488431902 1105 94-95 2.304099910965707 1105 96-97 2.3529074119313513 1105 98-99 2.595624687376798 1105 100-101 3.0836202206258 1106 1 -0.07541417568791076 1106 2 -0.09692199093004561 1106 3 -0.045541520152220016 1106 4 -0.10821779610294868 1106 5 -0.17489172978105216 1106 6 -0.12741262557813116 1106 7 -0.13107369988404827 1106 8 -0.10669357085804165 1106 9 -0.021680503842880228 1106 10-11 -0.30256446192948516 1106 12-13 -0.42988646533547836 1106 14-15 -0.4677824799156909 1106 16-17 -0.26992468824812477 1106 18-19 0.07976247741273568 1106 20-21 -0.003495364163306647 1106 22-23 -0.03382272737460568 1106 24-25 -0.08671876840784165 1106 26-27 -0.059533015083907515 1106 28-29 0.16438579659321562 1106 30-31 0.23440139646572788 1106 32-33 0.09583964908814124 1106 34-35 0.0761936088475963 1106 36-37 0.0037448533195387768 1106 38-39 -0.07093343912114847 1106 40-41 0.04626950443208955 1106 42-43 0.19436157078933292 1106 44-45 0.2509644178860384 1106 46-47 0.37997831948660377 1106 48-49 0.3876828523264919 1106 50-51 0.2229770161588931 1106 52-53 0.3224164417164701 1106 54-55 0.4217837872199013 1106 56-57 0.14579785188551142 1106 58-59 -0.12547585719543264 1106 60-61 0.253972230049321 1106 62-63 0.16996699051859565 1106 64-65 0.30371197861993693 1106 66-67 0.4997948634791598 1106 68-69 0.4286070120820362 1106 70-71 0.2988071474428864 1106 72-73 0.285878040101462 1106 74-75 0.39606649363779667 1106 76-77 0.6464861476612178 1106 78-79 0.7554521916345784 1106 80-81 0.627351473357237 1106 82-83 0.6666150734441736 1106 84-85 0.766216720370533 1106 86-87 0.9401703082421591 1106 88-89 1.1640519317865916 1106 90-91 1.331637193630705 1106 92-93 1.4204585255581144 1106 94-95 1.4726283532627242 1106 96-97 1.5600344552237786 1106 98-99 1.5153026864095018 1106 100-101 2.007122878305669 1107 1 0.1502189997353902 1107 2 0.17916015436205157 1107 3 0.19035042901772314 1107 4 0.13766860255084623 1107 5 0.16716176170483266 1107 6 0.15411178319801877 1107 7 0.16912502736968804 1107 8 0.17926509011807923 1107 9 0.2025286649526592 1107 10-11 0.2182064085393023 1107 12-13 0.20728374540811956 1107 14-15 0.33380358300815516 1107 16-17 0.23752186309766898 1107 18-19 0.41743554966449636 1107 20-21 0.3946808277601619 1107 22-23 0.3412108632226065 1107 24-25 0.37101751284021134 1107 26-27 0.42929173059566494 1107 28-29 0.4305532884053207 1107 30-31 0.40638936135079007 1107 32-33 0.4486550398597302 1107 34-35 0.359598504332439 1107 36-37 0.4020970658397758 1107 38-39 0.3972386632571201 1107 40-41 0.37002726840725586 1107 42-43 0.4841742027314382 1107 44-45 0.5314527987664448 1107 46-47 0.5994437331995499 1107 48-49 0.6789709769209082 1107 50-51 0.40944164834039043 1107 52-53 0.5598765877196854 1107 54-55 0.6916617932116438 1107 56-57 0.7771144976839537 1107 58-59 0.714773863225183 1107 60-61 0.848875788364353 1107 62-63 0.9589246024119547 1107 64-65 0.8789129648046625 1107 66-67 1.1211524105969417 1107 68-69 1.1911536135350325 1107 70-71 1.1082589841004697 1107 72-73 1.0805828711627576 1107 74-75 1.2208105596901113 1107 76-77 1.271953922397099 1107 78-79 1.353889950164831 1107 80-81 1.3216902402508168 1107 82-83 1.570682674741093 1107 84-85 1.9631824479868776 1107 86-87 2.0515744773486695 1107 88-89 1.8363096482361847 1107 90-91 2.1349278693503067 1107 92-93 2.2661996485460527 1107 94-95 2.38640349047078 1107 96-97 2.77642698936884 1107 98-99 3.1271513075228192 1107 100-101 3.7070533795067178 1108 1 0.15385883245407683 1108 2 0.08821581108761833 1108 3 0.284931173364658 1108 4 0.05890317158183933 1108 5 0.11493096290911353 1108 6 0.1890070493564977 1108 7 0.22826944934320892 1108 8 0.17616402585020552 1108 9 0.262390300978538 1108 10-11 0.2966927322853792 1108 12-13 0.2783005192946604 1108 14-15 0.3724730880275118 1108 16-17 0.4116247163970854 1108 18-19 0.4790433216567749 1108 20-21 0.37667841188125095 1108 22-23 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-2.015922520123137 2316 82-83 -2.098210952361491 2316 84-85 -2.2873829484740433 2316 86-87 -2.4454846409791067 2316 88-89 -2.513361410390093 2316 90-91 -2.4915915482138864 2316 92-93 -2.638923545009977 2316 94-95 -2.73449066512487 2316 96-97 -2.9614518399279 2316 98-99 -3.6042101999580893 2316 100-101 -3.286742749737172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7291.0 3 1298.0 4 1306.0 5 1551.0 6 1842.0 7 2222.0 8 2663.0 9 3431.0 10 4392.0 11 5582.0 12 7474.0 13 9316.0 14 10622.0 15 12971.0 16 16163.0 17 20703.0 18 18645.0 19 15021.0 20 15664.0 21 17038.0 22 18766.0 23 20999.0 24 24376.0 25 27444.0 26 32164.0 27 38075.0 28 45518.0 29 54528.0 30 65776.0 31 82101.0 32 102879.0 33 133694.0 34 176984.0 35 242287.0 36 326051.0 37 367656.0 38 184669.0 39 10372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43543424691742 18.005003745741867 14.336391177953365 24.223170829387346 2 15.837756557603635 19.251209469867682 39.202976663930556 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28.056323528581455 22.93557715290181 22-23 23.454441164208685 25.00414081402757 28.161487982698922 23.379930039064817 24-25 23.45228048637891 25.28195375951286 27.997043123391485 23.26872263071674 26-27 23.091639514287703 25.744925752125603 27.89316362390614 23.27027110968055 28-29 23.014036174231908 25.892026026171116 28.04215934770803 23.05177845188895 30-31 23.191496170603678 25.465462017113456 27.91583799163999 23.42720382064288 32-33 23.123683186339633 25.501465701548433 27.854564946179178 23.52028616593276 34-35 23.657973240643614 25.52663864703881 27.572462080087945 23.24292603222963 36-37 23.60600021315395 25.484813486763397 27.521680650679492 23.387505649403156 38-39 23.22991924602017 25.569309911981737 27.647346372657694 23.5534244693404 40-41 23.438385426553747 25.543261566515625 27.610030182570323 23.408322824360305 42-43 23.39497758683074 25.55797663685302 27.414997226290904 23.632048550025335 44-45 23.332195431469096 25.67508910100969 27.39229358903266 23.60042187848855 46-47 23.446344764822708 26.045951985062416 26.892920105999245 23.614783144115634 48-49 23.71995815354149 25.93562742157201 26.67492897349583 23.66948545139067 50-51 23.55768846155475 26.24506531501749 26.637040125477117 23.560206097950644 52-53 23.697347842518766 26.145596390682456 26.44500012472214 23.71205564207664 54-55 23.583938983326814 26.053212182158752 26.478259304390917 23.884589530123524 56-57 23.506102796066173 26.424224889622778 26.568391538647905 23.501280775663144 58-59 23.670994734354164 26.04977787393964 26.514443485438928 23.76478390626727 60-61 23.522381445447476 26.055515751056024 26.913975707389724 23.508127096106776 62-63 23.36087898978457 26.181882243141892 26.915181267887352 23.54205749918619 64-65 23.36540730065187 26.191777187268233 26.693880181656564 23.748935330423336 66-67 23.365096898436175 26.250374296657476 26.600901793260284 23.783627011646065 68-69 23.139604573355463 26.03422207419283 27.020930477620592 23.805242874831116 70-71 23.119921041811523 26.185031439321595 26.815699620168964 23.879347898697922 72-73 23.327028756398676 26.197916666666664 26.47658837699488 23.998466199939777 74-75 23.249424730574408 26.560967501894435 26.32403719271814 23.86557057481302 76-77 23.422485331832657 26.38086864911702 26.38510341413814 23.811542604912187 78-79 23.47121794413349 26.397933142368892 26.34782379577595 23.783025117721674 80-81 23.44745752983039 26.32823523489096 26.451715956231038 23.77259127904761 82-83 23.429986917047685 26.373086659387386 26.431374246295398 23.765552177269537 84-85 23.430408472012104 26.26673741730079 26.256999796780097 24.045854313907018 86-87 23.542822141938604 26.147003387420888 26.457260185490757 23.852914285149755 88-89 23.514277413610387 26.238330535916134 26.2441880570085 24.003203993464982 90-91 23.46126685519995 26.217942716431804 26.35195628762883 23.96883414073942 92-93 23.354541932597442 25.87915638246799 26.55403817641957 24.212263508515 94-95 23.34277604403974 26.008297322364644 26.461199303452087 24.187727330143527 96-97 23.418683786158475 25.973786758349103 26.331475297613093 24.276054157879322 98-99 23.179442953381685 26.096248708797674 26.221116883245198 24.503191454575436 100-101 23.706668755216967 25.21773960058357 26.764795603131226 24.310796041068237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2675.0 1 1543.0 2 412.5 3 397.0 4 348.5 5 375.5 6 574.0 7 1035.0 8 1707.5 9 2072.5 10 1960.0 11 1884.0 12 2003.5 13 2167.5 14 2368.5 15 2494.0 16 2596.5 17 2726.0 18 2871.0 19 3095.0 20 3383.5 21 3878.5 22 4606.5 23 5321.5 24 6178.5 25 7759.0 26 9650.0 27 11730.0 28 13709.5 29 15787.5 30 17971.5 31 20440.5 32 23054.0 33 24226.5 34 25458.5 35 27233.5 36 30110.5 37 34684.5 38 40048.5 39 43740.5 40 46796.0 41 52657.5 42 57934.5 43 62297.5 44 68065.0 45 73981.0 46 78162.5 47 81918.0 48 85843.5 49 87885.0 50 88296.5 51 87299.0 52 87276.0 53 89195.5 54 91112.5 55 87335.5 56 78827.0 57 71909.0 58 66254.0 59 59001.0 60 49354.5 61 40404.5 62 32637.0 63 25360.0 64 19649.0 65 15308.5 66 11332.5 67 8652.0 68 6220.5 69 4090.5 70 3076.5 71 2235.0 72 1715.0 73 1324.5 74 967.5 75 746.5 76 517.0 77 319.0 78 190.0 79 116.5 80 83.0 81 58.0 82 44.0 83 33.5 84 21.5 85 17.5 86 14.5 87 11.5 88 11.5 89 4.0 90 2.5 91 4.5 92 4.0 93 4.5 94 5.5 95 4.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3345332828684585 2 0.2856493486368379 3 0.29128438428313425 4 0.2848980105506651 5 0.2853675968545231 6 0.21492965127581903 7 0.20478658711248565 8 0.21286347153884372 9 0.21229996797421405 10-11 0.2131921819515443 12-13 0.21582186525314928 14-15 0.21197125756151347 16-17 0.203753497243998 18-19 0.19720276830517852 20-21 0.19581748870879734 22-23 0.20417612491747023 24-25 0.20279084532108904 26-27 0.2108442504322542 28-29 0.21582186525314928 30-31 0.21863938307629746 32-33 0.21662016196970793 34-35 0.2157749066227635 36-37 0.2023682176476168 38-39 0.19093379114867384 40-41 0.18640228331644387 42-43 0.1982123788584733 44-45 0.2008655414752711 46-47 0.20776846014198413 48-49 0.21708974827356595 50-51 0.2124878024957573 52-53 0.22591797078609688 54-55 0.21016335029166006 56-57 0.1816829409626707 58-59 0.16383866141606568 60-61 0.18158902370189908 62-63 0.17515569133904413 64-65 0.1819412134297926 66-67 0.18222296521210746 68-69 0.1798515543776244 70-71 0.18088464424611206 72-73 0.19224863279947632 74-75 0.19842369269520935 76-77 0.20044291380179888 78-79 0.19638099227342695 80-81 0.19257734321217693 82-83 0.18158902370189908 84-85 0.1764705329898466 86-87 0.18198817206017842 88-89 0.19072247731193773 90-91 0.18797539743436825 92-93 0.1832560550805951 94-95 0.1835378068629099 96-97 0.1916616499196538 98-99 0.19217819485389762 100-101 0.17175119063607344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2129534.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.38812534562249 #Duplication Level Percentage of deduplicated Percentage of total 1 65.25993796869614 26.35726554727248 2 15.451382721935758 12.48104764273454 3 6.83418115874167 8.280592958218504 4 3.6975121014281513 5.973423288777447 5 2.193152535030224 4.428865974343521 6 1.4061025695051517 3.4073908095585552 7 1.0113502955357683 2.8592579753101672 8 0.7110757338369916 2.2975212694751126 9 0.5230737339180904 1.901337077743796 >10 2.6429834410884494 18.999752319282486 >50 0.1797961627203861 5.016492989709948 >100 0.08560424121648835 6.147856212676273 >500 0.0030312346973872684 0.7983580432924445 >1k 5.829297494975507E-4 0.4841472958497731 >5k 2.3317189979902024E-4 0.5666905957549134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6733 0.31617245838760966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5302 0.248974658305526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3462 0.162570778395649 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2984 0.14012455307123528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.6958630385802715E-5 0.0 10-11 0.0 0.0 0.0 4.6958630385802715E-5 0.0 12-13 0.0 0.0 0.0 4.6958630385802715E-5 0.0 14-15 0.0 0.0 0.0 4.6958630385802715E-5 0.0 16-17 0.0 0.0 0.0 4.6958630385802715E-5 0.0 18-19 0.0 0.0 0.0 4.6958630385802715E-5 0.0 20-21 0.0 0.0 0.0 7.043794557870407E-5 0.0 22-23 0.0 0.0 0.0 1.643552063503095E-4 0.0 24-25 0.0 0.0 0.0 4.6958630385802715E-4 0.0 26-27 0.0 0.0 0.0 5.635035646296326E-4 0.0 28-29 0.0 0.0 0.0 0.0015731141179243908 0.0 30-31 0.0 0.0 0.0 0.004038442213179033 0.0 32-33 0.0 0.0 0.0 0.007442942916149731 0.0 34-35 0.0 0.0 0.0 0.010659609097577218 0.0 36-37 0.0 0.0 0.0 0.014251944322091124 0.0 38-39 0.0 0.0 0.0 0.01885389009989979 0.0 40-41 0.0 0.0 0.0 0.02369062902963747 0.0 42-43 0.0 0.0 0.0 0.03141532372810202 0.0 44-45 0.0 0.0 0.0 0.038506076916358226 0.0 46-47 0.0 0.0 0.0 0.045878581886929254 0.0 48-49 0.0 0.0 0.0 0.05442505261714535 0.0 50-51 0.0 2.3479315192901357E-5 0.0 0.06649342062629665 0.0 52-53 0.0 4.6958630385802715E-5 0.0 0.09887139627730762 0.0 54-55 0.0 4.6958630385802715E-5 0.0 0.1502676172345687 0.0 56-57 0.0 4.6958630385802715E-5 0.0 0.18870325620534822 0.0 58-59 0.0 4.6958630385802715E-5 0.0 0.24303439156172196 0.0 60-61 0.0 4.6958630385802715E-5 0.0 0.30682769094083495 0.0 62-63 0.0 4.6958630385802715E-5 0.0 0.3512270759706114 0.0 64-65 0.0 4.6958630385802715E-5 0.0 0.39032013576679214 0.0 66-67 0.0 4.6958630385802715E-5 0.0 0.5057444492550953 0.0 68-69 0.0 4.6958630385802715E-5 0.0 0.6230001493284446 0.0 70-71 0.0 4.6958630385802715E-5 0.0 0.6749129152199496 0.0 72-73 0.0 4.6958630385802715E-5 0.0 0.7890928250030289 0.0 74-75 0.0 4.6958630385802715E-5 0.0 0.9604918259112087 0.0 76-77 0.0 4.6958630385802715E-5 0.0 1.1286506813227684 0.0 78-79 0.0 4.6958630385802715E-5 0.0 1.2976078334508863 0.0 80-81 0.0 4.6958630385802715E-5 0.0 1.5044606003003473 0.0 82-83 0.0 4.6958630385802715E-5 0.0 1.735708375635233 0.0 84-85 0.0 4.6958630385802715E-5 0.0 1.985598727233282 0.0 86-87 0.0 4.6958630385802715E-5 0.0 2.3260253182151587 0.0 88-89 0.0 4.6958630385802715E-5 0.0 2.7149132157551836 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4355 0.0 42.26119 1 GTATCAA 7045 0.0 39.69312 1 ATCAACG 8625 0.0 32.298584 3 TCAACGC 8795 0.0 31.729824 4 TATCAAC 8800 0.0 31.711052 2 AACGTAC 60 0.00392837 31.704992 1 CAACGCA 8930 0.0 31.142204 5 AACGCAG 9140 0.0 30.404484 6 ACGCAGA 10465 0.0 26.100965 7 CGCAGAG 10560 0.0 25.686214 8 GTACATG 6235 0.0 24.560562 1 TACATGG 6330 0.0 23.28162 2 GAGTACT 6575 0.0 23.22561 12-13 GCAGAGT 11495 0.0 23.099367 9 GTATAGG 330 1.8189894E-12 21.617039 1 AGAGTAC 9730 0.0 21.259956 10-11 AGTACTT 6900 0.0 21.133492 12-13 ACATGGG 6540 0.0 21.00705 3 GTACTTT 7240 0.0 20.959875 14-15 CAGAGTA 10670 0.0 20.433147 10-11 >>END_MODULE