FastQCFastQC Report
Thu 26 May 2016
SRR937572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129534
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90030.4227685493633819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73250.3439719675760049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49780.2337600620605259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45050.044.7257961
GTATCAA72900.040.4809841
ATCAACG92750.031.907133
TATCAAC94000.031.58392
TCAACGC94600.031.3333664
CAACGCA96350.030.7149565
AACGCAG98300.030.2477866
ACGCAGA115650.025.4266537
CGCAGAG117050.024.960198
GTACATG61750.023.2411161
GCAGAGT126550.022.5609029
TACATGG62900.022.429492
GAGTACT79650.022.09767712-13
CTATGCG1353.7944285E-421.1135679
ACATGGG62650.020.850923
AGTACTT82100.020.54136812-13
GTACTTT85450.020.51101114-15
AGAGTAC113000.020.1793410-11
CAGAGTA119050.019.97187610-11
CTACGGT1250.00603119419.0008744
Produced by FastQC (version 0.11.5)