##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2129534 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19615934753801 33.0 31.0 34.0 31.0 34.0 2 32.411368872250925 34.0 31.0 34.0 31.0 34.0 3 32.535125525114886 34.0 31.0 34.0 31.0 34.0 4 36.17003109600504 37.0 35.0 37.0 35.0 37.0 5 35.99263359965138 37.0 35.0 37.0 35.0 37.0 6 36.08436681452374 37.0 35.0 37.0 35.0 37.0 7 36.04357760899803 37.0 35.0 37.0 35.0 37.0 8 36.0379998628808 37.0 35.0 37.0 35.0 37.0 9 37.699470870152815 39.0 37.0 39.0 35.0 39.0 10-11 37.6571489349313 39.0 37.0 39.0 35.0 39.0 12-13 37.572011998869236 39.0 37.0 39.0 35.0 39.0 14-15 38.83467251520756 40.0 38.0 41.0 35.0 41.0 16-17 38.79901471401725 40.0 38.0 41.0 35.0 41.0 18-19 38.75347634740746 40.0 38.0 41.0 35.0 41.0 20-21 38.654371331943985 40.0 38.0 41.0 34.0 41.0 22-23 38.50266865896482 40.0 38.0 41.0 34.0 41.0 24-25 38.442187586579976 40.0 38.0 41.0 34.0 41.0 26-27 38.36310995738974 40.0 38.0 41.0 34.0 41.0 28-29 38.24350867372862 40.0 38.0 41.0 34.0 41.0 30-31 38.332101295400776 40.0 38.0 41.0 34.0 41.0 32-33 38.272122210774754 40.0 38.0 41.0 34.0 41.0 34-35 38.14226539703053 40.0 38.0 41.0 34.0 41.0 36-37 37.98189040419172 40.0 37.0 41.0 33.0 41.0 38-39 37.83807138087488 40.0 37.0 41.0 33.0 41.0 40-41 37.65369395370067 40.0 37.0 41.0 33.0 41.0 42-43 37.449096140282336 40.0 37.0 41.0 33.0 41.0 44-45 37.15497592431021 40.0 36.0 41.0 32.0 41.0 46-47 36.81101264408082 39.5 36.0 41.0 31.0 41.0 48-49 36.482826994074756 39.0 36.0 41.0 30.5 41.0 50-51 36.2175628564747 39.0 35.0 40.5 30.0 41.0 52-53 35.967628128970944 39.0 35.0 40.0 29.5 41.0 54-55 35.726615776033626 38.5 35.0 40.0 29.0 41.0 56-57 35.40927897840561 38.0 35.0 40.0 28.0 41.0 58-59 35.11681593249979 38.0 34.0 40.0 28.0 41.0 60-61 34.7478455380379 37.5 34.0 40.0 27.0 41.0 62-63 34.35113104557147 37.0 34.0 39.5 26.5 41.0 64-65 33.91550146651802 36.0 33.0 39.0 26.0 40.5 66-67 33.46767978346436 36.0 33.0 39.0 26.0 40.0 68-69 33.014143939472206 35.0 32.5 38.0 25.0 40.0 70-71 32.58849682606618 35.0 32.0 37.5 24.0 39.5 72-73 32.163321881688674 35.0 32.0 37.0 23.5 39.0 74-75 31.681014484859126 35.0 31.0 36.0 22.0 39.0 76-77 30.462202528816164 33.5 29.5 35.0 20.5 37.0 78-79 31.051235152855035 34.0 31.0 35.0 21.0 37.0 80-81 30.882321202666873 34.0 31.0 35.0 20.0 37.0 82-83 30.56782164548676 34.0 31.0 35.0 19.5 36.5 84-85 30.21990303981998 34.0 30.5 35.0 18.0 36.0 86-87 29.82392462388485 34.0 30.0 35.0 16.0 35.5 88-89 29.418569508634285 34.0 29.5 35.0 8.5 35.0 90-91 28.940615881220964 34.0 29.0 35.0 4.5 35.0 92-93 28.52922517320691 33.5 29.0 35.0 2.0 35.0 94-95 28.077384770564827 33.0 28.5 35.0 2.0 35.0 96-97 27.525923042318176 33.0 27.0 35.0 2.0 35.0 98-99 26.70788280440697 33.0 25.5 34.5 2.0 35.0 100-101 24.267813521643703 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28540232634125573 1101 2 0.2148251038050546 1101 3 0.18430843048090395 1101 4 0.14857582089052812 1101 5 0.03233991932596325 1101 6 0.10530332235932605 1101 7 0.025676837859172963 1101 8 0.10897597837332995 1101 9 0.2661259275377148 1101 10-11 0.19194072793904127 1101 12-13 0.24675602579163325 1101 14-15 0.29128068409110597 1101 16-17 0.13616997610704118 1101 18-19 0.27249601965009873 1101 20-21 0.3290251221421272 1101 22-23 0.32233193010588934 1101 24-25 0.07810571533251931 1101 26-27 0.17995993343475902 1101 28-29 0.14564517303848135 1101 30-31 0.22059251520222034 1101 32-33 0.2351457877697456 1101 34-35 0.19982629455210343 1101 36-37 0.2627669120752856 1101 38-39 0.31576749608959176 1101 40-41 0.1903851275250119 1101 42-43 0.11298407241539365 1101 44-45 0.23673785708619732 1101 46-47 0.38101927787625556 1101 48-49 0.43290102237513395 1101 50-51 0.5322399011805601 1101 52-53 0.44150903896069593 1101 54-55 0.3709368279017511 1101 56-57 0.29100488089912346 1101 58-59 0.39996348160526907 1101 60-61 0.5047446219966787 1101 62-63 0.6196891231110726 1101 64-65 0.5411319722416437 1101 66-67 0.6939523761557496 1101 68-69 0.5041448727975322 1101 70-71 0.5833394848284499 1101 72-73 0.4300967643880469 1101 74-75 0.44619895865848136 1101 76-77 0.3559932917230597 1101 78-79 0.42044161891209697 1101 80-81 0.3832784387158874 1101 82-83 0.46155685272010416 1101 84-85 0.691362876959154 1101 86-87 0.615507163213465 1101 88-89 0.7352138709629195 1101 90-91 0.711226643171365 1101 92-93 0.8273922630150388 1101 94-95 0.9361794100931853 1101 96-97 1.041623593562111 1101 98-99 1.3786670326104087 1101 100-101 2.0446094323342763 1102 1 0.1850050415700224 1102 2 0.22137563469368615 1102 3 0.1880819096996902 1102 4 0.10243892257468445 1102 5 -0.061472396294483644 1102 6 0.0455676671402756 1102 7 0.015173997739843514 1102 8 0.07840309995699357 1102 9 0.24883937215227547 1102 10-11 0.12052220347334242 1102 12-13 0.13071827232810307 1102 14-15 0.1844385690009247 1102 16-17 0.2270471559867957 1102 18-19 0.2720309993813146 1102 20-21 0.13960140289280076 1102 22-23 0.16326159721651834 1102 24-25 0.12841607461073323 1102 26-27 0.1268927497792447 1102 28-29 0.10562780868581001 1102 30-31 0.19848408446669197 1102 32-33 0.1297246659122422 1102 34-35 0.20673251460014797 1102 36-37 0.25787657511970963 1102 38-39 0.3011277078641541 1102 40-41 0.13148169573144486 1102 42-43 0.3130904990930148 1102 44-45 0.4956673250357895 1102 46-47 0.49500100485137466 1102 48-49 0.5310656289698557 1102 50-51 0.6664560780355586 1102 52-53 0.5037037105731983 1102 54-55 0.49565862483218126 1102 56-57 0.620140169329936 1102 58-59 0.5037738885991416 1102 60-61 0.5544870611384667 1102 62-63 0.6151406792641083 1102 64-65 0.4930931897794366 1102 66-67 0.6622177250402359 1102 68-69 0.6266251745264952 1102 70-71 0.6356242353170103 1102 72-73 0.6440776615255217 1102 74-75 0.6506001063239388 1102 76-77 0.6340842294810898 1102 78-79 0.8890295671800175 1102 80-81 0.7993677458429502 1102 82-83 0.8244733793516659 1102 84-85 0.8862971330719418 1102 86-87 0.9021872317580524 1102 88-89 0.9300397295336893 1102 90-91 1.019208229074085 1102 92-93 1.3825492890428954 1102 94-95 1.023144320408484 1102 96-97 1.4842067121749558 1102 98-99 1.7363103559441981 1102 100-101 2.3650264464954525 1103 1 0.18772687347217953 1103 2 0.26883855428775405 1103 3 0.21420212827892726 1103 4 0.09194711929599464 1103 5 0.060494816820266806 1103 6 0.10532827083530094 1103 7 0.12096374533287246 1103 8 0.042363547523997624 1103 9 0.15246934873307083 1103 10-11 0.17805147747803574 1103 12-13 0.20189703891670518 1103 14-15 0.2909959460501028 1103 16-17 0.2434093730047593 1103 18-19 0.23750198559000069 1103 20-21 0.19572812680640794 1103 22-23 0.21840471457275612 1103 24-25 0.3334642140850903 1103 26-27 0.29204367353672467 1103 28-29 0.2763225783969787 1103 30-31 0.2166690961762967 1103 32-33 0.16607647439519013 1103 34-35 0.3035045676836816 1103 36-37 0.21549171953812873 1103 38-39 0.14393541018005607 1103 40-41 0.19559176598905026 1103 42-43 0.29931610408910814 1103 44-45 0.103230426779227 1103 46-47 0.2294155247576981 1103 48-49 0.21190091390350574 1103 50-51 0.2783452271378266 1103 52-53 0.21196028096091624 1103 54-55 0.17333622046059105 1103 56-57 0.22519351307180813 1103 58-59 0.23833152586169604 1103 60-61 0.22604573925451632 1103 62-63 0.36583024470777303 1103 64-65 0.12987044713046458 1103 66-67 0.15703947887838865 1103 68-69 -0.042922054201063986 1103 70-71 0.21636584343566767 1103 72-73 0.32167068780706387 1103 74-75 0.3330239939117625 1103 76-77 0.22393070358985767 1103 78-79 0.29648077717221 1103 80-81 0.40015879450285397 1103 82-83 0.28363028801677004 1103 84-85 0.48103691969697593 1103 86-87 0.4427948299886033 1103 88-89 0.452799292375218 1103 90-91 0.5699797617308633 1103 92-93 0.8365071344761503 1103 94-95 0.8489796053421301 1103 96-97 0.975580638794785 1103 98-99 1.1426907878984025 1103 100-101 1.9758487207603501 1104 1 0.18387055637887073 1104 2 0.2601955595175909 1104 3 0.15921176219029576 1104 4 0.0983247686216231 1104 5 -0.022857784335798215 1104 6 0.02069848828644183 1104 7 0.06500448301522965 1104 8 0.11592534218406314 1104 9 0.24319326835872346 1104 10-11 0.12420204174998162 1104 12-13 0.1817029844162903 1104 14-15 0.23993621579961655 1104 16-17 0.1742778013009314 1104 18-19 0.19760245352284045 1104 20-21 0.1425916005195873 1104 22-23 0.22955527884470683 1104 24-25 0.18682232033336987 1104 26-27 0.16369244865371257 1104 28-29 0.17165563647570536 1104 30-31 0.16951522884901493 1104 32-33 0.1653332869468045 1104 34-35 0.13828436399526112 1104 36-37 0.2803306955909761 1104 38-39 0.13138792875974303 1104 40-41 0.09904621135101621 1104 42-43 0.2711671913448157 1104 44-45 0.19435621813622816 1104 46-47 0.3640824666789797 1104 48-49 0.32101872287680067 1104 50-51 0.284933446240494 1104 52-53 0.2057381504902267 1104 54-55 0.27300866747408037 1104 56-57 0.3640057894274378 1104 58-59 0.41048814573507997 1104 60-61 0.45156872301449624 1104 62-63 0.4629538340278927 1104 64-65 0.3685340859964086 1104 66-67 0.36930207177681496 1104 68-69 0.42560808617879076 1104 70-71 0.47675945752870774 1104 72-73 0.4544975526570525 1104 74-75 0.5011481337411077 1104 76-77 0.25419357174289914 1104 78-79 0.4557128168088731 1104 80-81 0.5087013213401761 1104 82-83 0.5890624377552811 1104 84-85 0.5538390116320144 1104 86-87 0.5611322921410924 1104 88-89 0.7965468838973777 1104 90-91 0.655841308661774 1104 92-93 0.877076911519918 1104 94-95 1.1367968362807481 1104 96-97 1.2535258631272903 1104 98-99 1.5880678867148106 1104 100-101 2.2261336092822326 1105 1 0.20798661373191862 1105 2 0.2605052209544212 1105 3 0.17379087286767003 1105 4 0.13227179462067085 1105 5 0.030235076560529706 1105 6 -0.019541859034823972 1105 7 0.12053084489996735 1105 8 0.03424666440711377 1105 9 0.11653861280233713 1105 10-11 0.20967485410140796 1105 12-13 0.11589054541020971 1105 14-15 0.2896431321972912 1105 16-17 0.23871240330778676 1105 18-19 0.32923358732160324 1105 20-21 0.3044835380618238 1105 22-23 0.24224670591474506 1105 24-25 0.23443824005912717 1105 26-27 0.18636185535490313 1105 28-29 0.23721002428442262 1105 30-31 0.3357924727996746 1105 32-33 0.23225612807484453 1105 34-35 0.1865024031815139 1105 36-37 0.308489555035969 1105 38-39 0.2620306482752923 1105 40-41 0.27189046728775423 1105 42-43 0.3862533335263336 1105 44-45 0.37155293760173436 1105 46-47 0.30583327367544655 1105 48-49 0.3973987494013329 1105 50-51 0.42762011891271356 1105 52-53 0.37842131992195505 1105 54-55 0.25865007327445255 1105 56-57 0.43140563428392653 1105 58-59 0.4357990583292235 1105 60-61 0.49292885613763104 1105 62-63 0.5206462620237957 1105 64-65 0.6496972869572986 1105 66-67 0.6391282234671323 1105 68-69 0.6113095475305457 1105 70-71 0.6111493932192147 1105 72-73 0.5897226358590189 1105 74-75 0.5624720458598169 1105 76-77 0.5591146862738405 1105 78-79 0.6035370542284895 1105 80-81 0.6205375823816404 1105 82-83 0.7281973875838723 1105 84-85 0.6520001231601782 1105 86-87 0.7918316265253971 1105 88-89 0.8669010239769506 1105 90-91 0.7933888526399535 1105 92-93 1.0011932816622995 1105 94-95 1.1662631551256801 1105 96-97 1.3543144050285498 1105 98-99 1.9076150303226456 1105 100-101 2.4593227467343795 1106 1 0.041363427708624556 1106 2 0.13583936672059593 1106 3 0.0834169512471945 1106 4 0.10327348143138693 1106 5 -0.0031705656783529435 1106 6 0.010009194387990306 1106 7 0.019227497724308762 1106 8 -0.02521251977688621 1106 9 0.1061711596720869 1106 10-11 0.1314895098181026 1106 12-13 -0.06527110847434159 1106 14-15 0.05840100934123882 1106 16-17 0.21401450443542558 1106 18-19 0.343496429597657 1106 20-21 0.17622174527746637 1106 22-23 0.2152182743389801 1106 24-25 0.189263936285478 1106 26-27 0.027748786622140642 1106 28-29 0.08506946625503531 1106 30-31 0.1214967817909951 1106 32-33 0.23806719693627088 1106 34-35 -0.04192955973724111 1106 36-37 0.13475921960365156 1106 38-39 0.1323951947543094 1106 40-41 0.1796707030123983 1106 42-43 0.3251780691282917 1106 44-45 0.19037341495963744 1106 46-47 0.30569523752338057 1106 48-49 0.3906544846768867 1106 50-51 0.3254496902769617 1106 52-53 0.4629451592270257 1106 54-55 0.33677779081465786 1106 56-57 0.2683008066221362 1106 58-59 0.2820366127395957 1106 60-61 0.36819986562714035 1106 62-63 0.3453955931366366 1106 64-65 0.2147240781811135 1106 66-67 0.3081808159856081 1106 68-69 0.04270915370312167 1106 70-71 0.33020576795804146 1106 72-73 0.3382008133410537 1106 74-75 0.3903707454907597 1106 76-77 0.3100657508944167 1106 78-79 0.4040779415195104 1106 80-81 0.37296430856317997 1106 82-83 0.3335140577051625 1106 84-85 0.3134945290478157 1106 86-87 0.4236058214187466 1106 88-89 0.5557382026669089 1106 90-91 0.6672978160852416 1106 92-93 0.7794090921772288 1106 94-95 0.7548208664475631 1106 96-97 0.9381916931229561 1106 98-99 1.2812916999197874 1106 100-101 1.882628996987517 1107 1 0.05923362542669253 1107 2 0.16482291818256556 1107 3 0.05135920006286909 1107 4 0.003588910340774021 1107 5 -0.12396147315840977 1107 6 0.0025635231451843765 1107 7 0.054723517898615626 1107 8 0.029037321511204084 1107 9 0.1566626678517622 1107 10-11 0.12605076674597626 1107 12-13 0.11441658404819322 1107 14-15 0.1329469055383754 1107 16-17 0.09957852723361071 1107 18-19 0.20920063519910315 1107 20-21 0.23140332225772653 1107 22-23 0.19817728031546267 1107 24-25 0.16439882673612516 1107 26-27 0.08952057474497366 1107 28-29 0.04736380085611103 1107 30-31 0.172344291627617 1107 32-33 0.10669481128218905 1107 34-35 0.1334761384015124 1107 36-37 0.0742266164819867 1107 38-39 0.12574777691779104 1107 40-41 0.1588113821937398 1107 42-43 0.06668639548924915 1107 44-45 -0.04061107997272728 1107 46-47 0.07988105425307879 1107 48-49 0.1359058890278817 1107 50-51 0.03162525556710705 1107 52-53 0.006615575914715066 1107 54-55 -0.05209732609235829 1107 56-57 0.1203392131428842 1107 58-59 0.16132726146936704 1107 60-61 0.05390643577192833 1107 62-63 -0.11522874605270772 1107 64-65 -0.03998740645873511 1107 66-67 0.12432803609232224 1107 68-69 0.0828277681159264 1107 70-71 0.22128055558918192 1107 72-73 0.05123003393358516 1107 74-75 0.1586672064206418 1107 76-77 0.07114463393811832 1107 78-79 0.1341389150542298 1107 80-81 0.15484251873881405 1107 82-83 0.3012493356358199 1107 84-85 0.1888976162143905 1107 86-87 0.4045432308414796 1107 88-89 0.5778632582600807 1107 90-91 0.6701858740265259 1107 92-93 1.0295135310646373 1107 94-95 0.9108559379647296 1107 96-97 0.9016467368758079 1107 98-99 1.1045564595401913 1107 100-101 1.914381059068809 1108 1 0.08013235068452218 1108 2 0.15665788802983371 1108 3 0.034709996662336096 1108 4 0.032590896120034074 1108 5 -0.05783136343724493 1108 6 -0.002843607358038014 1108 7 -0.0637746564656183 1108 8 -0.11597489514920767 1108 9 0.0838202581955656 1108 10-11 0.033094906866949714 1108 12-13 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2316 46-47 -0.6473732420310654 2316 48-49 -0.6087340067314244 2316 50-51 -0.6211297423371462 2316 52-53 -0.4971544931538574 2316 54-55 -0.48145010432572377 2316 56-57 -0.5317478788695809 2316 58-59 -0.46016053763037235 2316 60-61 -0.5003889371801549 2316 62-63 -0.5819414280638924 2316 64-65 -0.5973334600734503 2316 66-67 -0.6252560034170926 2316 68-69 -0.6889415902206011 2316 70-71 -0.5540492969794713 2316 72-73 -0.3569556858193046 2316 74-75 -0.5247782038904383 2316 76-77 -0.4370766224174716 2316 78-79 -0.5008086001171677 2316 80-81 -0.5346031102590523 2316 82-83 -0.6628532284667443 2316 84-85 -0.8881389044788506 2316 86-87 -0.6053614581676854 2316 88-89 -0.8036964341205071 2316 90-91 -0.6221081503570502 2316 92-93 -0.7319666139975958 2316 94-95 -0.763022836660312 2316 96-97 -0.9255487873346411 2316 98-99 -1.1808256956180827 2316 100-101 -1.4775334526218238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 18.0 5 31.0 6 70.0 7 195.0 8 511.0 9 935.0 10 1544.0 11 2620.0 12 3879.0 13 5715.0 14 7340.0 15 9906.0 16 13320.0 17 19081.0 18 17134.0 19 10971.0 20 9943.0 21 10246.0 22 10604.0 23 11556.0 24 13566.0 25 15957.0 26 19908.0 27 24599.0 28 30901.0 29 39323.0 30 49716.0 31 63209.0 32 82109.0 33 109783.0 34 152713.0 35 224908.0 36 350669.0 37 494190.0 38 304792.0 39 17563.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.869498506281886 18.62362766556687 14.880805569532003 23.62606825861924 2 16.318781479891843 19.706424034554036 38.6427734894113 25.332020996142816 3 19.492339638625165 22.480599041856106 30.03704096764832 27.99002035187041 4 14.023255792112266 16.056329694665592 34.80315411728576 35.11726039593639 5 14.850662080276237 36.13346433727629 33.55751790110643 15.458355681341049 6 34.7054332074529 34.337277545228204 16.011906830320623 14.945382416998273 7 29.918376508663396 32.19281777139975 19.441436483286953 18.447369236649898 8 27.430555229453958 34.26092281222089 18.843653118475682 19.46486883984947 9 26.723546090365307 16.07182604269291 19.620067113274548 37.58456075366723 10-11 26.023604225149725 25.68010184387758 26.238674752316705 22.057619178655987 12-13 26.298890971864797 22.75395504528907 27.370162559781203 23.576991423064932 14-15 23.300191497294716 24.405550697946126 25.214788775384662 27.079469029374504 16-17 23.263399410387436 27.59437041155483 26.07547003241085 23.06676014564689 18-19 23.191857936994666 25.884512761946983 28.3522357473513 22.571393553707058 20-21 23.483991666711667 25.13717219022118 28.717936316039467 22.66089982702768 22-23 23.471264427189197 24.765987040799388 28.7558824613606 23.006866070650815 24-25 23.48721794519303 24.925940178776663 28.67428165436621 22.91256022166409 26-27 23.16750555351153 25.458832401936878 28.536138738117266 22.837523306434328 28-29 23.023080206641318 25.671910652659534 28.539807944354674 22.765201196344474 30-31 23.196743693853502 25.20380675637506 28.516768610155435 23.082680939616004 32-33 23.248114376196856 25.108732708658327 28.53196990515296 23.111183009991855 34-35 23.68738888414085 25.126436112313772 28.246931018711134 22.93924398483424 36-37 23.728570408897355 25.163862294626238 28.13362278660288 22.97394450987353 38-39 23.333555604183825 25.218686341706682 28.336809837269563 23.110948216839926 40-41 23.482531859082787 25.190816394572707 28.17365207599409 23.152999670350415 42-43 23.511904063043747 25.26042412700798 27.90362158722406 23.32405022272421 44-45 23.401997807971135 25.35972658807044 27.887509661738203 23.35076594222022 46-47 23.68243474863515 25.74659526450388 27.38397226811124 23.186997718749737 48-49 23.95559310158936 25.636289441727627 27.09578715343357 23.312330303249443 50-51 23.755009311896405 25.99204802553047 27.001071595945405 23.25187106662772 52-53 24.06861034790257 25.925918969088528 26.702474204438246 23.302996478570652 54-55 23.775718067896545 25.898788185584266 26.753951803540115 23.571541942979074 56-57 23.681683410548974 26.202610524180407 26.896917353749693 23.218788711520926 58-59 23.943829964677718 25.827763257125735 26.804338413944084 23.424068364252463 60-61 23.664449593197386 25.86760765500809 27.292614252695664 23.175328499098864 62-63 23.461470913354752 25.92010740377942 27.36716107843312 23.25126060443271 64-65 23.618312738843333 25.960303991389665 27.057562828299524 23.363820441467475 66-67 23.557289998656984 25.92060046939847 26.968599703033618 23.553509828910926 68-69 23.427355468379467 25.67686169838096 27.40411752054675 23.491665312692824 70-71 23.361073361589906 25.927104239706907 27.142839701080142 23.56898269762305 72-73 23.543688579535647 25.968751357398865 26.663363907336468 23.824196155729016 74-75 23.444934510273967 26.34409195445479 26.553551072829368 23.657422462441875 76-77 23.696357982544537 26.026233908451335 26.77106822431574 23.506339884688387 78-79 23.646910544748287 26.120667714157182 26.680766778083843 23.551654963010687 80-81 23.55872223688375 26.243534970561633 26.648388802432834 23.549353990121784 82-83 23.603583043304617 26.258745765792035 26.6403087441105 23.49736244679285 84-85 23.511811892544237 26.12405803634844 26.486110181718715 23.878019889388607 86-87 23.53655776334165 25.93954827675914 26.715516164569337 23.80837779532987 88-89 23.5502931627295 26.169528169073608 26.50392057605091 23.77625809214598 90-91 23.555894098235246 26.068352487110737 26.559408957078524 23.816344457575497 92-93 23.342350450358857 25.675679165951504 26.796428310632443 24.1855420730572 94-95 23.34304124752176 25.819921165851305 26.806592428202602 24.03044515842433 96-97 23.461752665137066 25.659745277605335 26.723569569680507 24.154932487577092 98-99 23.201768277922426 25.79893821616465 26.527761179171268 24.471532326741656 100-101 23.141791458341356 25.714560119326606 26.833740199748085 24.30990822258395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 302.0 1 235.0 2 242.0 3 366.5 4 340.5 5 293.5 6 542.0 7 1284.5 8 2168.0 9 2584.0 10 2361.0 11 2096.0 12 2183.5 13 2434.0 14 2748.5 15 2974.5 16 3089.0 17 3264.5 18 3458.0 19 3566.0 20 3833.0 21 4281.5 22 4925.0 23 5660.0 24 6552.0 25 8100.5 26 9788.0 27 11622.0 28 13644.5 29 15822.5 30 17978.5 31 20445.0 32 23009.5 33 24284.0 34 25328.0 35 26939.0 36 29840.0 37 34166.0 38 39513.5 39 43413.0 40 46475.0 41 52583.5 42 58142.0 43 61982.0 44 67220.0 45 73294.0 46 77606.5 47 81884.0 48 86067.5 49 87458.0 50 88086.0 51 87346.0 52 87749.0 53 89935.0 54 91687.0 55 87380.5 56 78466.5 57 71762.5 58 66710.0 59 59810.0 60 49597.5 61 40324.0 62 32724.5 63 25648.5 64 19404.5 65 14591.5 66 10846.0 67 8695.0 68 6236.0 69 3852.5 70 2808.0 71 1974.5 72 1556.0 73 1222.5 74 912.5 75 730.5 76 501.5 77 291.5 78 179.0 79 108.5 80 61.0 81 41.5 82 27.0 83 16.0 84 8.0 85 2.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044751574757669985 2 0.0 3 0.0 4 0.0 5 0.01577809980962971 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004789780299351877 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.173965759645068E-4 22-23 2.113138367361122E-4 24-25 2.5827246712191497E-4 26-27 0.0011974450748379693 28-29 0.0022070556281327276 30-31 2.113138367361122E-4 32-33 0.0 34-35 0.0 36-37 9.391726077160543E-5 38-39 0.0 40-41 0.0 42-43 0.010283940054490794 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.6958630385802715E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.043794557870407E-5 74-75 7.043794557870407E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6435520635030951E-4 84-85 3.0523109750771766E-4 86-87 0.0 88-89 0.0 90-91 0.006832480721134296 92-93 0.0030757902902700776 94-95 0.0 96-97 0.0 98-99 2.113138367361122E-4 100-101 0.011833574857222284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2129534.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.17715968079192 #Duplication Level Percentage of deduplicated Percentage of total 1 63.02964416563069 24.062927899347773 2 16.34321439290701 12.478750111508553 3 7.175393113447568 8.218083859936275 4 3.8668738126176856 5.905050360391121 5 2.3932198635727753 4.568316844143045 6 1.5660807026763872 3.5873107835449938 7 1.0622662672897973 2.8388016236888967 8 0.7718245721770071 2.35728559500484 9 0.5670471326210225 1.9483424035747168 >10 2.925986143423532 19.80153853440575 >50 0.19553031277700714 5.154556247850664 >100 0.09734783950812159 6.543568259494127 >500 0.004704975363386094 1.246722621763177 >1k 6.190757057086961E-4 0.5169349229043448 >5k 2.476302822834785E-4 0.7718099324416736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9003 0.4227685493633819 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7325 0.3439719675760049 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4978 0.2337600620605259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.6958630385802715E-5 0.0 10-11 0.0 0.0 0.0 9.391726077160543E-5 0.0 12-13 0.0 0.0 0.0 9.391726077160543E-5 0.0 14-15 0.0 0.0 0.0 9.391726077160543E-5 0.0 16-17 0.0 0.0 0.0 9.391726077160543E-5 0.0 18-19 0.0 0.0 0.0 9.391726077160543E-5 0.0 20-21 0.0 0.0 0.0 1.1739657596450679E-4 0.0 22-23 0.0 0.0 0.0 2.1131383673611223E-4 0.0 24-25 0.0 0.0 0.0 5.165449342438298E-4 0.0 26-27 0.0 0.0 0.0 6.104621950154353E-4 0.0 28-29 0.0 0.0 0.0 0.0015965934331172923 0.0 30-31 0.0 0.0 0.0 0.00396800426760033 0.0 32-33 0.0 0.0 0.0 0.007442942916149731 0.0 34-35 0.0 0.0 0.0 0.010636129782384315 0.0 36-37 0.0 0.0 0.0 0.01418150637651242 0.0 38-39 0.0 0.0 0.0 0.018947807360671396 0.0 40-41 0.0 0.0 0.0 0.02371410834483037 0.0 42-43 0.0 0.0 0.0 0.03150924098887362 0.0 44-45 0.0 0.0 0.0 0.038646952807515636 0.0 46-47 0.0 0.0 0.0 0.04616033366924407 0.0 48-49 0.0 0.0 0.0 0.05484768029061757 0.0 50-51 0.0 0.0 0.0 0.06670473446303275 0.0 52-53 0.0 0.0 0.0 0.09936446189635854 0.0 54-55 0.0 0.0 0.0 0.15127722778786346 0.0 56-57 0.0 0.0 0.0 0.1900180978561507 0.0 58-59 0.0 0.0 0.0 0.24479534020118957 0.0 60-61 0.0 0.0 0.0 0.30894082930819605 0.0 62-63 0.0 0.0 0.0 0.35397415584818087 0.0 64-65 0.0 0.0 0.0 0.3939124709913061 0.0 66-67 0.0 0.0 0.0 0.509618536261924 0.0 68-69 0.0 0.0 0.0 0.6276020951062533 0.0 70-71 0.0 0.0 0.0 0.679820092095266 0.0 72-73 0.0 0.0 0.0 0.7948217779100968 0.0 74-75 0.0 0.0 0.0 0.968286958555252 0.0 76-77 0.0 0.0 0.0 1.138723307540523 0.0 78-79 0.0 0.0 0.0 1.3095588048840732 0.0 80-81 0.0 0.0 0.0 1.5199569483276623 0.0 82-83 0.0 0.0 0.0 1.7543509518983966 0.0 84-85 0.0 0.0 0.0 2.0066596729613146 0.0 86-87 0.0 0.0 0.0 2.3531674065781525 0.0 88-89 0.0 0.0 0.0 2.749592164295099 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4505 0.0 44.725796 1 GTATCAA 7290 0.0 40.480984 1 ATCAACG 9275 0.0 31.90713 3 TATCAAC 9400 0.0 31.5839 2 TCAACGC 9460 0.0 31.333366 4 CAACGCA 9635 0.0 30.714956 5 AACGCAG 9830 0.0 30.247786 6 ACGCAGA 11565 0.0 25.426653 7 CGCAGAG 11705 0.0 24.96019 8 GTACATG 6175 0.0 23.241116 1 GCAGAGT 12655 0.0 22.560902 9 TACATGG 6290 0.0 22.42949 2 GAGTACT 7965 0.0 22.097677 12-13 CTATGCG 135 3.7944285E-4 21.113567 9 ACATGGG 6265 0.0 20.85092 3 AGTACTT 8210 0.0 20.541368 12-13 GTACTTT 8545 0.0 20.511011 14-15 AGAGTAC 11300 0.0 20.17934 10-11 CAGAGTA 11905 0.0 19.971876 10-11 CTACGGT 125 0.006031194 19.000874 4 >>END_MODULE