FastQCFastQC Report
Thu 26 May 2016
SRR937571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519082
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79780.5251856055170162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.40280906494843594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42890.28234157208103317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19460.1281036836721125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.1037468681743316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.056.0089041
GTATCAA62850.043.9695051
ATCAACG81550.033.4621283
TATCAAC82250.033.2329522
TCAACGC82250.033.178444
CAACGCA83750.032.6409655
AACGCAG85050.032.112366
ACGCAGA98550.027.6679467
CGCAGAG99400.027.3348678
GTGTAGC9750.026.831121
GCAGAGT106900.025.0615989
TGGTATC15150.024.7885652
GTACATG62650.024.5223561
ATAGGAC3750.024.087293
GAGTACT65500.023.06179812-13
TACATGG64000.022.951732
GTGGTAT17300.022.8198471
GCGTTCG753.0345886E-522.16736634-35
GTACTTT68550.021.79173714-15
AGAGTAC98500.021.77353110-11
Produced by FastQC (version 0.11.5)