FastQCFastQC Report
Thu 26 May 2016
SRR937571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519082
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83880.5521755902578004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73420.4833182145532631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.3458667800684887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.12922278060038891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40100.054.160891
GTATCAA73300.043.374661
TATCAAC97900.032.2750632
ATCAACG97750.032.1301573
TCAACGC99400.031.5490074
CAACGCA101450.030.9114995
AACGCAG104200.030.2215466
ACGCAGA119150.026.3099967
CGCAGAG119350.026.226118
GTACCGT1109.481967E-525.9077366
GCAGAGT127700.024.064919
GAGTACT79200.023.35894212-13
TAAGGTG5300.023.3091075
GGACCGA2653.092282E-1123.3006696
TGGTATC17450.022.6001222
AGAGTAC117950.022.06751810-11
AGTACTT83450.021.74242212-13
GTACTTT85050.021.58470214-15
CAGAGTA123850.020.8053310-11
GTGGTAT20500.020.4005931
Produced by FastQC (version 0.11.5)