##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937571_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1519082 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.318105935031817 31.0 31.0 33.0 30.0 34.0 2 31.645561595753225 31.0 31.0 34.0 30.0 34.0 3 31.710728584763693 31.0 31.0 34.0 30.0 34.0 4 35.55119802617634 37.0 35.0 37.0 33.0 37.0 5 35.415949237763336 37.0 35.0 37.0 33.0 37.0 6 35.48388039618664 37.0 35.0 37.0 33.0 37.0 7 35.40444228817141 37.0 35.0 37.0 33.0 37.0 8 35.361366930817425 37.0 35.0 37.0 33.0 37.0 9 36.918598864314106 39.0 37.0 39.0 33.0 39.0 10-11 36.78172211901662 38.5 36.5 39.0 32.5 39.0 12-13 36.624708870225575 38.0 35.0 39.0 32.0 39.0 14-15 37.54165114193967 39.0 36.0 40.0 32.0 41.0 16-17 37.450228164114904 39.0 36.0 40.0 32.0 41.0 18-19 37.3985637378364 39.0 36.0 40.0 32.0 41.0 20-21 37.256414071129804 39.0 36.0 40.0 32.0 41.0 22-23 37.17492735744351 39.0 36.0 40.0 31.5 41.0 24-25 37.185869821378965 39.0 36.0 40.0 31.0 41.0 26-27 36.9567485494529 39.0 36.0 40.0 31.0 41.0 28-29 36.726084240350424 38.5 35.5 40.0 30.0 41.0 30-31 36.72583705158773 38.5 35.5 40.0 30.0 41.0 32-33 36.583322690940975 38.0 35.0 40.0 30.0 41.0 34-35 36.35910898819155 38.0 35.0 40.0 30.0 41.0 36-37 36.11110295560081 38.0 35.0 40.0 30.0 41.0 38-39 35.90708171119137 38.0 35.0 40.0 29.5 41.0 40-41 35.621186348070744 38.0 34.5 40.0 29.0 41.0 42-43 35.288161863546534 38.0 34.0 40.0 27.0 41.0 44-45 34.862089077482324 38.0 33.5 40.0 26.5 41.0 46-47 34.3831547605725 38.0 33.0 40.0 24.5 41.0 48-49 33.92758290862508 37.5 33.0 40.0 23.5 41.0 50-51 33.54024173810235 37.0 32.5 40.0 22.5 41.0 52-53 33.17077188723189 37.0 32.0 39.0 21.5 40.0 54-55 32.82507428828727 36.5 31.0 39.0 19.5 40.0 56-57 32.405146002651605 36.0 31.0 39.0 18.0 40.0 58-59 32.000742553726525 36.0 30.5 39.0 16.5 40.0 60-61 31.517325595326653 35.0 29.5 38.5 13.5 40.0 62-63 30.960348750100387 34.5 29.0 38.0 8.5 40.0 64-65 30.383094197679917 34.0 28.0 38.0 8.0 40.0 66-67 29.791426664261706 34.0 28.0 37.5 7.0 39.0 68-69 29.19204229923072 33.5 26.5 37.0 4.0 39.0 70-71 28.665359736999058 33.0 26.0 36.0 2.0 39.0 72-73 27.994388058050852 32.0 26.0 36.0 2.0 38.5 74-75 27.353160988017763 32.0 25.0 35.0 2.0 38.0 76-77 26.521698960293126 30.5 24.0 34.0 2.0 36.0 78-79 27.089769676686316 32.0 25.0 35.0 2.0 37.0 80-81 26.77717661061088 32.0 25.0 35.0 2.0 36.0 82-83 26.217102500062538 31.0 23.5 34.0 2.0 36.0 84-85 25.556589440201385 31.0 21.5 34.0 2.0 35.5 86-87 24.83775793538466 31.0 19.0 34.0 2.0 35.0 88-89 24.033450465478495 30.0 16.0 34.0 2.0 35.0 90-91 23.038069702623034 29.0 7.0 34.0 2.0 35.0 92-93 22.144279571478037 29.0 2.0 34.0 2.0 35.0 94-95 21.17565114983918 29.0 2.0 33.0 2.0 34.5 96-97 20.02315543203066 27.0 2.0 33.0 2.0 34.0 98-99 18.481525026298776 25.0 2.0 32.0 2.0 34.0 100-101 15.76162412562324 11.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3197604494388173 1101 2 0.20518473554466254 1101 3 0.29969095027824366 1101 4 0.15308481262639617 1101 5 0.17951194502906986 1101 6 0.1315455820066873 1101 7 0.2827765160839988 1101 8 0.19263181399031026 1101 9 0.24682462206260425 1101 10-11 0.2178289007419849 1101 12-13 0.3111827399970579 1101 14-15 0.26753281545476426 1101 16-17 0.34269231332228145 1101 18-19 0.3036946893522554 1101 20-21 0.30350645234531015 1101 22-23 0.3739529928212022 1101 24-25 0.2669997556107049 1101 26-27 0.13385354119284898 1101 28-29 -0.029342072538135255 1101 30-31 0.11448068505814746 1101 32-33 0.22728100668696527 1101 34-35 0.08861158666645963 1101 36-37 0.10944333108150772 1101 38-39 0.011233384072632191 1101 40-41 0.0794712477544337 1101 42-43 0.20233938163278253 1101 44-45 -0.21981303419558884 1101 46-47 -0.29114077727867027 1101 48-49 -0.5623671244617512 1101 50-51 -0.31841699455004147 1101 52-53 -0.22293848789950488 1101 54-55 -0.42957534124861496 1101 56-57 -0.4020296010619795 1101 58-59 -0.39914012231123763 1101 60-61 -0.2872407434962376 1101 62-63 -0.359184183409738 1101 64-65 -0.2741198129286495 1101 66-67 -0.12722849737300734 1101 68-69 -0.03117675530621966 1101 70-71 -0.2198336627562405 1101 72-73 -0.46837467003826916 1101 74-75 -0.5293067035730523 1101 76-77 -0.28251926970102303 1101 78-79 -0.3386060666997892 1101 80-81 -0.30849725405512984 1101 82-83 -0.20125431545609374 1101 84-85 -0.15171558368888327 1101 86-87 -0.34266622584795314 1101 88-89 -0.39234978071473137 1101 90-91 -0.45059426311512496 1101 92-93 -0.39571014479156474 1101 94-95 -0.5661326379546914 1101 96-97 -0.5431213395094971 1101 98-99 -0.471435849943699 1101 100-101 -0.3319039315482595 1102 1 0.2325693769673407 1102 2 0.23184111908317817 1102 3 0.12324808380085628 1102 4 0.03633058999040628 1102 5 0.04610931863911105 1102 6 -0.0021707424351617988 1102 7 -0.05842649426080726 1102 8 0.1018281542760775 1102 9 -0.02354478991979647 1102 10-11 8.176119489533562E-4 1102 12-13 0.1613577676082727 1102 14-15 0.32518531318515187 1102 16-17 0.23473085192303955 1102 18-19 0.26608706345380995 1102 20-21 0.14762074685717863 1102 22-23 0.24636823652266315 1102 24-25 0.23768796143131965 1102 26-27 0.1439271156737334 1102 28-29 0.12247116002632907 1102 30-31 0.007358402088051719 1102 32-33 0.04879223620984874 1102 34-35 -0.1357473923258894 1102 36-37 -0.1117085551545216 1102 38-39 0.1192309858004208 1102 40-41 0.11455862784082882 1102 42-43 -0.03290090818374125 1102 44-45 -0.14549948359623954 1102 46-47 -0.046108626842382705 1102 48-49 -0.230312676888353 1102 50-51 -0.16613881665868035 1102 52-53 -0.215600736290547 1102 54-55 -0.4255776788050625 1102 56-57 -0.41758024233596913 1102 58-59 -0.35542857562082375 1102 60-61 -0.4698256588122227 1102 62-63 -0.4878527881617529 1102 64-65 -0.5194396403802664 1102 66-67 -0.6474620217200204 1102 68-69 -0.8561744423251945 1102 70-71 -0.7228468626498454 1102 72-73 -0.6501227560464677 1102 74-75 -0.8406247341275304 1102 76-77 -0.8794284765220048 1102 78-79 -0.6359088778391069 1102 80-81 -0.4825333553521354 1102 82-83 -0.7413811029567476 1102 84-85 -0.5234201278292332 1102 86-87 -0.495351524540542 1102 88-89 -0.3402047076534451 1102 90-91 -0.291914548826103 1102 92-93 -0.15846478954659204 1102 94-95 -0.43002111323759706 1102 96-97 -0.63566298762413 1102 98-99 -0.5530860679421608 1102 100-101 -0.6542984307011306 1103 1 0.28863722139742976 1103 2 0.21656857254725992 1103 3 0.2101130296802225 1103 4 0.10203417221434563 1103 5 0.07065898910583002 1103 6 0.18234552730957176 1103 7 0.05700277981524948 1103 8 0.055464554771567975 1103 9 0.1733880479252221 1103 10-11 0.23517386860743272 1103 12-13 0.22334116791082437 1103 14-15 0.1576058588933833 1103 16-17 0.1889749036864785 1103 18-19 0.2692497511144154 1103 20-21 0.22649604849827654 1103 22-23 0.1243689187977779 1103 24-25 0.155866328836467 1103 26-27 0.18187447423446912 1103 28-29 0.1968162528185431 1103 30-31 0.36893764526600137 1103 32-33 0.3565171842702597 1103 34-35 0.23706439900978182 1103 36-37 0.17716662065715383 1103 38-39 0.4102445627954481 1103 40-41 0.3648634146717953 1103 42-43 0.29340153122938517 1103 44-45 0.1690207777374937 1103 46-47 0.06480354561663404 1103 48-49 -0.05451865826000102 1103 50-51 0.005693322693218761 1103 52-53 0.11596853840068633 1103 54-55 -0.05192737415804061 1103 56-57 -0.17085879792600878 1103 58-59 -0.08042259872310709 1103 60-61 0.027230631680385642 1103 62-63 0.0578528283045614 1103 64-65 -0.010337279540618027 1103 66-67 -0.08372434950847207 1103 68-69 0.2858272102345971 1103 70-71 0.03669153210222831 1103 72-73 0.07295726168149841 1103 74-75 0.016517798455300436 1103 76-77 -0.03364170007615641 1103 78-79 0.09522894173268526 1103 80-81 0.10907675797823302 1103 82-83 -0.14994016029861257 1103 84-85 0.10577820590955156 1103 86-87 -0.06996977204422805 1103 88-89 -0.014190452577622636 1103 90-91 -0.1262019138740449 1103 92-93 -0.11162985398523873 1103 94-95 -0.16134977410492723 1103 96-97 -0.2179213531837796 1103 98-99 -0.3405972178273764 1103 100-101 -0.2885963514397769 1104 1 0.35565544116498415 1104 2 0.2812099967779851 1104 3 0.18570296180375578 1104 4 0.10221953148958107 1104 5 0.11908112701872398 1104 6 0.12385033685816182 1104 7 0.27371435238935504 1104 8 0.17658390096393362 1104 9 0.12595286071934453 1104 10-11 0.29715031608850495 1104 12-13 0.21633703544818417 1104 14-15 0.11262405907580586 1104 16-17 0.16092794693603452 1104 18-19 0.02809208801224372 1104 20-21 -0.018419861342970023 1104 22-23 0.12294479998877961 1104 24-25 -0.04723637559033733 1104 26-27 0.1803356425155087 1104 28-29 -0.009035014899005489 1104 30-31 0.2035412142364379 1104 32-33 0.2201780929084265 1104 34-35 0.33544995469590333 1104 36-37 0.3977969680973814 1104 38-39 0.2470525082033035 1104 40-41 0.1537119208858826 1104 42-43 0.2663834933237652 1104 44-45 0.19160558135458672 1104 46-47 0.004117968107081538 1104 48-49 0.16786245999501404 1104 50-51 0.11592788261533116 1104 52-53 0.23159845217015373 1104 54-55 0.06569287854610906 1104 56-57 0.21318463629872753 1104 58-59 0.08533161068111283 1104 60-61 0.017051959328437505 1104 62-63 0.08926562970573926 1104 64-65 0.24951124346071651 1104 66-67 0.2873267984599899 1104 68-69 0.2622767897138587 1104 70-71 0.1605678352129054 1104 72-73 0.1197073190379534 1104 74-75 0.23800289696850996 1104 76-77 0.1900418293882744 1104 78-79 0.18732452276756106 1104 80-81 0.08912090845918996 1104 82-83 0.215357505713353 1104 84-85 0.2629051651221168 1104 86-87 0.21725037042360995 1104 88-89 0.14257173357065156 1104 90-91 0.3050133574919336 1104 92-93 0.12245757683728087 1104 94-95 0.09183071127057829 1104 96-97 -0.14769711742356506 1104 98-99 -0.019500394535619137 1104 100-101 -0.10036890371808838 1105 1 0.2408043710256642 1105 2 0.10258683523342427 1105 3 0.16367571359872457 1105 4 0.09609823762755809 1105 5 0.0156828002341598 1105 6 0.010534758389830756 1105 7 0.01712756360571177 1105 8 0.027713920182534935 1105 9 0.07214863376883329 1105 10-11 0.058367816986695686 1105 12-13 0.17558413127313344 1105 14-15 0.0077543976538620996 1105 16-17 -0.022678790407915983 1105 18-19 -0.01919342789584988 1105 20-21 -0.2108200329986829 1105 22-23 -0.2008397093667469 1105 24-25 -0.07554245881768651 1105 26-27 -0.25310963296053757 1105 28-29 -0.2041864540125573 1105 30-31 -0.1568979905585124 1105 32-33 -0.09768008868164202 1105 34-35 -0.010936364743400873 1105 36-37 -0.1074311172856568 1105 38-39 -0.11827403684063853 1105 40-41 -0.08765753238213136 1105 42-43 0.09711977369855873 1105 44-45 -0.0039707198926208775 1105 46-47 -0.12149045442829731 1105 48-49 -0.11101533368736938 1105 50-51 0.05195654797794447 1105 52-53 0.2244720314482862 1105 54-55 -0.03325473224595754 1105 56-57 0.07103474835967916 1105 58-59 0.029541010684535962 1105 60-61 0.017234450446249383 1105 62-63 -0.41468715642037424 1105 64-65 -0.42877152937775875 1105 66-67 -0.39086544167551196 1105 68-69 -0.35567912442407135 1105 70-71 -0.5660950438125916 1105 72-73 -0.6863523840718067 1105 74-75 -0.643519378353556 1105 76-77 -0.3922883968436821 1105 78-79 -0.42346897142408935 1105 80-81 -0.6238904230986542 1105 82-83 -0.36527362841191646 1105 84-85 -0.4222377767487586 1105 86-87 -0.5460561547744192 1105 88-89 -0.6696352826425453 1105 90-91 -0.28831694857988666 1105 92-93 -0.40325299795271263 1105 94-95 -0.49092330781115123 1105 96-97 -0.8036372033084476 1105 98-99 -0.9603843216304178 1105 100-101 -0.5326140862377198 1106 1 0.11342614853609945 1106 2 -0.009263285223486406 1106 3 -0.0021380346722814636 1106 4 -0.03456659597701872 1106 5 -0.0693790159934764 1106 6 -0.07051946096083128 1106 7 -0.08125855804813398 1106 8 -0.12516864883210133 1106 9 -0.1741571252623828 1106 10-11 -0.2662002022375489 1106 12-13 -0.5006820021668617 1106 14-15 -0.27207815349834163 1106 16-17 -0.28583248670993555 1106 18-19 -0.2519880511512085 1106 20-21 0.09496743307033029 1106 22-23 0.08095217498240714 1106 24-25 -0.057487662373070236 1106 26-27 0.11997874332914904 1106 28-29 0.09997946202485508 1106 30-31 -0.12390325463243101 1106 32-33 -0.08964275772589758 1106 34-35 -0.7110858671907039 1106 36-37 -0.12547370010169345 1106 38-39 -0.25360523991358974 1106 40-41 -0.5100021368590362 1106 42-43 -0.6456556741599329 1106 44-45 -0.4647863460090278 1106 46-47 -0.7452883662031482 1106 48-49 -0.7472606505241615 1106 50-51 -0.36780620992045954 1106 52-53 -0.2169876651041136 1106 54-55 -0.32671139523836246 1106 56-57 -0.34359923972592554 1106 58-59 -0.21139132428644203 1106 60-61 -0.3209219939221981 1106 62-63 -0.7403563105960629 1106 64-65 -0.8694375309105702 1106 66-67 -0.9148699927788577 1106 68-69 -0.46705463090798105 1106 70-71 -0.4883703741149077 1106 72-73 -0.49805830737632917 1106 74-75 -0.8087405748006127 1106 76-77 -0.7074620364744639 1106 78-79 -0.7193853447092167 1106 80-81 -1.0690581009519882 1106 82-83 -1.2748034647798256 1106 84-85 -1.2376318342077752 1106 86-87 -0.8845986643086832 1106 88-89 -1.0976563618387658 1106 90-91 -1.3073944455799165 1106 92-93 -1.3745299786215952 1106 94-95 -1.0910010170229754 1106 96-97 -0.986107287551949 1106 98-99 -1.4194184755160286 1106 100-101 -1.584547579976709 1107 1 0.1325264523508487 1107 2 0.15832032776588179 1107 3 0.016130372351916833 1107 4 0.002919905557938307 1107 5 -0.04235806281101162 1107 6 -0.1741028166705192 1107 7 0.053070106666460504 1107 8 0.02659110154461075 1107 9 -0.004464128887931906 1107 10-11 0.060854577023071954 1107 12-13 0.07524165706310271 1107 14-15 0.14322471776611678 1107 16-17 0.031075244040685845 1107 18-19 0.0746006024275161 1107 20-21 0.11288091812892986 1107 22-23 0.020293647497844347 1107 24-25 0.125182071501321 1107 26-27 -0.0017970754033740377 1107 28-29 -0.022857054175823066 1107 30-31 0.005981523593646898 1107 32-33 -0.05762248067753717 1107 34-35 -0.10513856070357974 1107 36-37 -0.05408343279043493 1107 38-39 -0.10450643560539419 1107 40-41 -0.3266221715840416 1107 42-43 -0.05878116116053178 1107 44-45 0.10568058173452499 1107 46-47 -0.002109086826479256 1107 48-49 -0.018465591223908007 1107 50-51 0.19017781006676415 1107 52-53 0.23267347353669976 1107 54-55 0.18781870706518333 1107 56-57 -0.007106093478320474 1107 58-59 -0.004148087661594246 1107 60-61 -0.08283076890283425 1107 62-63 -0.18976501292618764 1107 64-65 -0.07979989616324801 1107 66-67 -0.1550998551226037 1107 68-69 0.029288432944259313 1107 70-71 -0.003153106463837929 1107 72-73 -0.017581340300768744 1107 74-75 -0.28598643459326567 1107 76-77 -0.343437252382099 1107 78-79 -0.1818465348059668 1107 80-81 -0.3209988382829998 1107 82-83 -0.20644708282954483 1107 84-85 -0.03949606570679265 1107 86-87 0.14695044995565354 1107 88-89 0.13348308103449114 1107 90-91 0.09827419291849893 1107 92-93 0.051861491782140945 1107 94-95 0.023139523551076735 1107 96-97 0.15444956021403655 1107 98-99 0.20581880105458694 1107 100-101 0.0526231727484614 1108 1 0.19754243952070993 1108 2 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-0.12962426278797068 2316 90-91 -0.1286736339583321 2316 92-93 0.254591819890031 2316 94-95 0.3880504329114487 2316 96-97 0.6501265008696784 2316 98-99 0.6082641921020162 2316 100-101 0.8275458429338141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 28.0 5 88.0 6 157.0 7 412.0 8 906.0 9 1545.0 10 2572.0 11 3946.0 12 5999.0 13 8213.0 14 10862.0 15 14389.0 16 18636.0 17 23184.0 18 21330.0 19 16948.0 20 16878.0 21 17894.0 22 19542.0 23 22006.0 24 25592.0 25 30214.0 26 35608.0 27 43155.0 28 51822.0 29 60852.0 30 72395.0 31 85762.0 32 102195.0 33 122874.0 34 148147.0 35 173776.0 36 184389.0 37 137641.0 38 38135.0 39 978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54400324447885 21.943290091171963 16.803872306582186 20.708834357766996 2 17.175373021337887 22.29241081126628 38.16712988502267 22.36508628237317 3 20.605668423429414 25.264666423537374 30.72934838277328 23.400316770259934 4 14.55405303992806 18.78884747498818 36.40830448915858 30.24879499592517 5 13.720495929155987 37.993314912906854 34.02862102299217 14.25756813494499 6 31.519957447985032 37.59948442546222 16.57250892315227 14.308049203400476 7 27.910409049676055 33.576396797539566 20.299957474316727 18.213236678467652 8 26.334325599276404 36.183300177343945 18.90549687245323 18.576877350926416 9 25.873323494057594 16.97907025427199 20.293111234284915 36.8544950173855 10-11 25.106643354341635 27.61108353597765 26.56923721036784 20.713035899312874 12-13 26.01220778246132 24.701437956170743 27.747030837422017 21.539323423945923 14-15 22.608127803502377 26.03220234325731 26.459401138319066 24.900268714921246 16-17 21.54603898933698 29.507887000175103 27.034748617915294 21.91132539257262 18-19 21.51552714073368 27.297341420673803 30.210614041901625 20.976517396690898 20-21 22.31617516368438 26.297724546798655 30.662235481692235 20.723864807824725 22-23 21.930717592478306 26.340645360902787 30.60768988971889 21.120947156900016 24-25 21.802106277623665 26.492319683386896 30.739139135712584 20.966434903276856 26-27 21.654739389375187 26.785059513504013 30.449907739421633 21.110293357699167 28-29 21.468257468734077 26.820065750928528 30.674177422179156 21.037499358158236 30-31 21.602125494212952 26.52131353014518 30.798008270784592 21.078552704857277 32-33 21.48702308367817 26.674893126243347 30.558719015826664 21.279364774251817 34-35 21.959051585102056 26.68022529396043 30.309390803129787 21.051332317807727 36-37 21.96379128973946 26.449559668273338 30.343095369440228 21.243553672546973 38-39 21.72575279017196 26.722388916464023 30.357874031816586 21.193984261547435 40-41 21.906917038329247 26.74769185194938 30.225400325856196 21.11999078386518 42-43 21.949315903611602 26.654182066680796 30.014951235665972 21.381550794041626 44-45 21.971756626699545 26.924945460482054 29.749480278220663 21.35381763459774 46-47 22.118654555843595 27.186353337081208 29.249869328976313 21.445122778098877 48-49 22.31291661674617 27.394604109587238 28.83517808781883 21.45730118584777 50-51 22.216970512454232 27.482650706150164 28.710201292622784 21.590177488772824 52-53 22.544602595514924 27.509706520122023 28.494840963160645 21.450849921202412 54-55 22.406760146522945 27.47838411353718 28.48850241215157 21.62635332778831 56-57 22.36857523162015 27.759725939745188 28.41657000741237 21.455128821222292 58-59 22.54878275168819 27.456549416028892 28.38414911110789 21.610518721175026 60-61 22.365744574683923 27.492261773887122 28.717639995734267 21.424353655694688 62-63 22.317096772919434 27.46533761837742 28.717936227274105 21.49962938142905 64-65 22.267165301148985 27.38640837031839 28.629659228402417 21.71676710013021 66-67 22.212230807816827 27.643208200742293 28.412916485087703 21.731644506353177 68-69 22.28958015433005 27.480478341524684 28.494840963160645 21.735100540984618 70-71 22.332040008373475 27.483407742307524 28.435002192113394 21.749550057205603 72-73 22.46764822438815 27.47600195381158 28.240542643517596 21.81580717828267 74-75 22.35169003384939 27.735303295016333 28.001055900866444 21.911950770267833 76-77 22.315977675991157 27.659171789278 28.23646123119094 21.7883893035399 78-79 22.390726767876913 27.644261468439492 28.120108065265732 21.844903698417863 80-81 22.275459784264445 27.790336532195102 28.091274862054842 21.842928821485607 82-83 22.393393865864248 27.829077338613743 28.10440972882804 21.673119066693964 84-85 22.412845389518143 27.69465374482747 27.994769209298774 21.897731656355614 86-87 22.530910115451306 27.517573113235493 28.042199170288374 21.90931760102483 88-89 22.512181699210444 27.683726092469 27.912021865837396 21.89207034248316 90-91 22.478957157199993 27.67924683575555 27.914808170251987 21.92698783679247 92-93 22.522491683187283 27.61297024511516 27.9115427518899 21.95299531980766 94-95 22.34013700379571 27.60489558825659 27.877329861060822 22.17763754688687 96-97 22.60707453580518 27.504868071638 27.735270380400795 22.152787012156026 98-99 22.441073301781124 27.48317308720898 27.60864463410002 22.467108976909877 100-101 22.7747592343562 27.093085075739744 28.023656016630976 22.10849967327308 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 680.0 1 457.0 2 331.5 3 452.5 4 385.0 5 334.0 6 612.5 7 1447.5 8 2368.0 9 2705.5 10 2326.0 11 1864.0 12 1822.5 13 1924.5 14 2105.5 15 2331.5 16 2546.0 17 2764.0 18 3004.0 19 3233.5 20 3562.0 21 4081.5 22 4864.5 23 5867.5 24 7111.0 25 8734.0 26 10867.5 27 13122.0 28 14908.5 29 16896.5 30 19459.5 31 22493.5 32 25667.0 33 28487.5 34 31391.5 35 34138.5 36 37186.0 37 40122.0 38 42115.0 39 43946.5 40 46082.5 41 48799.5 42 51477.0 43 53873.5 44 55697.0 45 57023.5 46 57248.5 47 57694.5 48 59014.5 49 58057.5 50 55525.5 51 52371.5 52 48951.5 53 46949.5 54 45566.0 55 42469.5 56 38519.0 57 34700.5 58 30790.0 59 26957.5 60 22618.0 61 18366.5 62 14753.0 63 11521.5 64 8893.5 65 6911.5 66 5270.5 67 4017.5 68 2857.5 69 1860.5 70 1316.0 71 1030.0 72 830.5 73 666.0 74 528.5 75 434.5 76 335.5 77 230.5 78 165.0 79 115.5 80 71.5 81 53.5 82 41.0 83 23.5 84 13.0 85 8.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0127708708285662 2 0.0 3 0.0 4 0.0 5 0.03146637245388992 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033572907848292586 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.253776952132933E-4 24-25 2.63316924300334E-4 26-27 9.874384661262526E-4 28-29 0.001053267697201336 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.6080461752558453E-4 42-43 0.009479409274812025 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.6457307768770875E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.924630796757516E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01033518927878811 92-93 0.001349499237039212 94-95 0.0 96-97 0.0 98-99 8.228653884385438E-4 100-101 0.015107808531731666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1519082.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.372392116667505 #Duplication Level Percentage of deduplicated Percentage of total 1 80.52539724808321 49.42036255259781 2 11.603283959079686 14.242425859553535 3 3.413062260613406 6.284033861308967 4 1.4247514118808429 3.4976160927490696 5 0.7818438206266003 2.399181276674458 6 0.4648851144439249 1.7118666919712644 7 0.3028102508568475 1.3008932616773028 8 0.22805835923912174 1.119718963896576 9 0.16955332133182333 0.9365303629313987 >10 0.9851488410478461 11.151095046314477 >50 0.0719371978142607 3.036317826634137 >100 0.02766015261646528 2.9064073953451004 >500 0.0010720415773586415 0.40068397435074393 >1k 2.1440831547172827E-4 0.21225065702060755 >5k 3.216124732075924E-4 1.3806161769747145 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8388 0.5521755902578004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7342 0.4833182145532631 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5254 0.3458667800684887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1963 0.12922278060038891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.58292310750835E-5 0.0 0.0 0.0 0.0 7 6.58292310750835E-5 0.0 0.0 0.0 0.0 8 6.58292310750835E-5 0.0 0.0 0.0 0.0 9 6.58292310750835E-5 0.0 0.0 0.0 0.0 10-11 6.58292310750835E-5 0.0 0.0 0.0 0.0 12-13 6.58292310750835E-5 0.0 0.0 0.0 0.0 14-15 6.58292310750835E-5 0.0 0.0 0.0 0.0 16-17 6.58292310750835E-5 0.0 0.0 0.0 0.0 18-19 1.31658462150167E-4 0.0 0.0 0.0 0.0 20-21 1.9748769322525052E-4 0.0 0.0 0.0 0.0 22-23 1.9748769322525052E-4 0.0 0.0 3.291461553754175E-5 0.0 24-25 1.9748769322525052E-4 0.0 0.0 2.63316924300334E-4 0.0 26-27 1.9748769322525052E-4 0.0 0.0 3.6206077091295927E-4 0.0 28-29 2.63316924300334E-4 0.0 0.0 6.58292310750835E-4 0.0 30-31 2.63316924300334E-4 0.0 0.0 0.0012178407748890448 0.0 32-33 2.962315398378758E-4 0.0 0.0 0.0028635715517661324 0.0 34-35 3.6206077091295927E-4 0.0 0.0 0.0045751315597183035 0.0 36-37 3.9497538645050104E-4 0.0 0.0 0.007504532342559519 0.0 38-39 3.9497538645050104E-4 0.0 0.0 0.011355542360451904 0.0 40-41 3.9497538645050104E-4 0.0 0.0 0.016062332382320375 0.0 42-43 3.9497538645050104E-4 0.0 0.0 0.02218445087230314 0.0 44-45 3.9497538645050104E-4 0.0 0.0 0.029557324752712492 0.0 46-47 4.2789000198804276E-4 0.0 0.0 0.038905075565374356 0.0 48-49 4.6080461752558453E-4 0.0 0.0 0.049174435613087375 0.0 50-51 4.6080461752558453E-4 0.0 0.0 0.060727465666764534 0.0 52-53 4.6080461752558453E-4 0.0 0.0 0.0732350195710304 0.0 54-55 4.6080461752558453E-4 0.0 0.0 0.09061393657485245 0.0 56-57 4.6080461752558453E-4 0.0 0.0 0.1105931082061403 0.0 58-59 4.6080461752558453E-4 0.0 0.0 0.1331725344648939 0.0 60-61 4.937192330631263E-4 0.0 0.0 0.1591750807395519 0.0 62-63 5.26633848600668E-4 0.0 0.0 0.18932486857194014 0.0 64-65 5.26633848600668E-4 0.0 0.0 0.22171285026088125 0.0 66-67 5.26633848600668E-4 0.0 0.0 0.2608483281350184 0.0 68-69 5.26633848600668E-4 0.0 0.0 0.30472351064656156 0.0 70-71 5.595484641382098E-4 0.0 0.0 0.3591313701301181 0.0 72-73 5.924630796757516E-4 0.0 0.0 0.41715983732280415 3.291461553754175E-5 74-75 5.924630796757516E-4 0.0 0.0 0.48068504531025974 6.58292310750835E-5 76-77 6.253776952132933E-4 0.0 0.0 0.5538213210346775 6.58292310750835E-5 78-79 6.58292310750835E-4 0.0 0.0 0.6363382621872947 6.58292310750835E-5 80-81 7.899507729010021E-4 0.0 0.0 0.7344896457202442 6.58292310750835E-5 82-83 7.899507729010021E-4 0.0 0.0 0.8399809885180656 6.58292310750835E-5 84-85 8.228653884385438E-4 0.0 0.0 0.9482700736365779 9.874384661262526E-5 86-87 8.557800039760855E-4 0.0 0.0 1.0627471064761482 1.31658462150167E-4 88-89 8.557800039760855E-4 0.0 6.58292310750835E-5 1.192397777078525 1.31658462150167E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4010 0.0 54.16089 1 GTATCAA 7330 0.0 43.37466 1 TATCAAC 9790 0.0 32.275063 2 ATCAACG 9775 0.0 32.130157 3 TCAACGC 9940 0.0 31.549007 4 CAACGCA 10145 0.0 30.911499 5 AACGCAG 10420 0.0 30.221546 6 ACGCAGA 11915 0.0 26.309996 7 CGCAGAG 11935 0.0 26.22611 8 GTACCGT 110 9.481967E-5 25.907736 6 GCAGAGT 12770 0.0 24.06491 9 GAGTACT 7920 0.0 23.358942 12-13 TAAGGTG 530 0.0 23.309107 5 GGACCGA 265 3.092282E-11 23.300669 6 TGGTATC 1745 0.0 22.600122 2 AGAGTAC 11795 0.0 22.067518 10-11 AGTACTT 8345 0.0 21.742422 12-13 GTACTTT 8505 0.0 21.584702 14-15 CAGAGTA 12385 0.0 20.80533 10-11 GTGGTAT 2050 0.0 20.400593 1 >>END_MODULE