FastQCFastQC Report
Thu 26 May 2016
SRR937570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502332
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79270.5276463524706922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61450.4090307601781763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43290.28815201966010173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20170.13425794032211255No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.10576889795331525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32550.054.3435671
GTATCAA59350.043.9853251
ATCAACG76400.034.155543
TCAACGC77800.033.60654
TATCAAC78300.033.3289572
CAACGCA79900.032.778345
AACGCAG81650.032.115796
ACGCAGA93250.028.0197377
CGCAGAG93800.027.906098
CGTAATA700.00836249227.162712
TGGTATC15350.025.7028262
GCAGAGT102400.025.144049
GTGGTAT17250.023.430741
GAGTACT62400.023.1782412-13
GTACATG61800.023.159771
TACATGG62750.022.5742682
AGAGTAC95650.021.7514710-11
ACATGGG62600.021.5638433
AGTACTT66600.021.43127412-13
CAGAGTA99300.021.40638210-11
Produced by FastQC (version 0.11.5)