##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937570_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1502332 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0330599361526 33.0 31.0 34.0 31.0 34.0 2 32.18826331330225 33.0 31.0 34.0 31.0 34.0 3 32.24089681907861 34.0 31.0 34.0 31.0 34.0 4 35.815881576109675 37.0 35.0 37.0 35.0 37.0 5 35.79711142410599 37.0 35.0 37.0 35.0 37.0 6 35.89005093414771 37.0 36.0 37.0 35.0 37.0 7 35.84450574174017 37.0 35.0 37.0 35.0 37.0 8 35.83694682666681 37.0 35.0 37.0 35.0 37.0 9 37.511641900725 39.0 37.0 39.0 35.0 39.0 10-11 37.41835925747438 39.0 37.0 39.0 35.0 39.0 12-13 37.307737903472734 39.0 37.0 39.0 35.0 39.0 14-15 38.5210023483491 40.0 38.0 41.0 34.5 41.0 16-17 38.453616777117176 40.0 38.0 41.0 34.0 41.0 18-19 38.401007234086734 40.0 38.0 41.0 34.0 41.0 20-21 38.236998879076 40.0 38.0 41.0 34.0 41.0 22-23 38.09828985869967 40.0 38.0 41.0 34.0 41.0 24-25 37.94929017021537 40.0 38.0 41.0 33.5 41.0 26-27 38.05409223793409 40.0 38.0 41.0 34.0 41.0 28-29 37.991548805457114 40.0 38.0 41.0 34.0 41.0 30-31 37.784530316867375 40.0 37.5 41.0 33.0 41.0 32-33 37.54758502115378 40.0 37.0 41.0 33.0 41.0 34-35 37.29026872888283 39.5 36.5 41.0 32.5 41.0 36-37 37.31527618395934 40.0 37.0 41.0 33.0 41.0 38-39 37.26570125644665 40.0 37.0 41.0 32.5 41.0 40-41 37.07496345681247 40.0 36.5 41.0 31.5 41.0 42-43 36.83036572475325 39.5 36.0 41.0 31.0 41.0 44-45 36.595549119635336 39.0 36.0 41.0 30.5 41.0 46-47 36.231526386977045 39.0 36.0 41.0 30.0 41.0 48-49 35.89412460095372 39.0 35.0 41.0 29.0 41.0 50-51 35.01318583375712 38.0 34.0 39.5 27.0 40.5 52-53 35.12184756764816 38.5 35.0 40.0 28.0 40.5 54-55 35.41072812134735 39.0 35.0 40.0 28.0 41.0 56-57 35.138352241714884 38.5 35.0 40.0 26.5 41.0 58-59 34.904844268776806 38.0 34.5 40.0 26.0 41.0 60-61 34.60453348527489 38.0 34.0 40.0 26.0 41.0 62-63 34.234017514104735 37.5 34.0 40.0 25.0 41.0 64-65 33.85882048708275 37.0 34.0 40.0 23.5 41.0 66-67 33.44433454123323 36.5 33.0 39.0 22.5 41.0 68-69 32.96023215907003 36.0 32.5 39.0 21.5 40.5 70-71 32.51088141635803 35.5 32.0 39.0 20.0 40.0 72-73 32.03709133533733 35.0 31.5 38.0 18.5 40.0 74-75 31.539831408769835 35.0 31.0 37.0 16.5 39.0 76-77 31.070159259071897 35.0 31.0 37.0 11.5 39.0 78-79 30.534628830378374 34.5 30.0 36.0 7.5 39.0 80-81 30.006113162736334 34.0 30.0 36.0 4.5 37.5 82-83 29.484906132599185 34.0 29.0 35.0 2.0 37.0 84-85 29.002869538823642 34.0 29.0 35.0 2.0 36.5 86-87 28.464291847607587 34.0 28.5 35.0 2.0 36.0 88-89 27.97293174877457 33.5 27.0 35.0 2.0 36.0 90-91 27.453959910326077 33.0 26.0 35.0 2.0 35.0 92-93 26.892679181432598 33.0 25.5 35.0 2.0 35.0 94-95 26.23895383976378 32.5 24.5 35.0 2.0 35.0 96-97 25.442721715306604 32.0 21.5 34.0 2.0 35.0 98-99 24.476123786220356 32.0 10.0 34.0 2.0 35.0 100-101 21.87212513612171 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1752368619376483 1101 2 0.19912082902685313 1101 3 0.1838903055350869 1101 4 0.19523423589227917 1101 5 0.1490381283836868 1101 6 0.15574272923279153 1101 7 0.19037285354666267 1101 8 0.14330723143046242 1101 9 0.25085925462888525 1101 10-11 0.10729477530845344 1101 12-13 0.29678229372954235 1101 14-15 0.3807569831343329 1101 16-17 0.41168541375569134 1101 18-19 0.4085051748595774 1101 20-21 0.39114962820275423 1101 22-23 0.36101311503665556 1101 24-25 0.49861692602998886 1101 26-27 0.5119813970771432 1101 28-29 0.362205959170133 1101 30-31 0.447867197406957 1101 32-33 0.5274564974718174 1101 34-35 0.4778180045417244 1101 36-37 0.5038664648883824 1101 38-39 0.4757036049600103 1101 40-41 0.5139066578403515 1101 42-43 0.6156839516522581 1101 44-45 0.614527791949385 1101 46-47 0.6183011231517881 1101 48-49 0.7649882520961597 1101 50-51 0.6385775170539816 1101 52-53 0.6207494686679453 1101 54-55 0.7204445159623205 1101 56-57 0.8026690057487116 1101 58-59 0.7347791984059739 1101 60-61 0.825319155642525 1101 62-63 0.7473908735783183 1101 64-65 0.826009774470883 1101 66-67 1.0002883804541085 1101 68-69 1.0364667121962086 1101 70-71 0.93922189732573 1101 72-73 0.8751263583127589 1101 74-75 0.8836023385619995 1101 76-77 0.9345254825276079 1101 78-79 0.9078553053673204 1101 80-81 1.1624955040043687 1101 82-83 1.2319369398599456 1101 84-85 1.1821690569731444 1101 86-87 1.2192232032610022 1101 88-89 1.2679542173671763 1101 90-91 1.317840387262386 1101 92-93 1.3723855850763194 1101 94-95 1.4712374732589986 1101 96-97 1.6815339898817783 1101 98-99 1.892499928726803 1101 100-101 2.2999727754112342 1102 1 0.12567993799513744 1102 2 0.1827711662178899 1102 3 0.06939154672110703 1102 4 0.11988200214817368 1102 5 -0.014825124956317381 1102 6 0.1449136091594383 1102 7 0.10933011402902082 1102 8 0.10333791594519681 1102 9 0.020690229318070408 1102 10-11 0.10158782675060252 1102 12-13 0.24802504262445524 1102 14-15 0.2959182396365563 1102 16-17 0.33510534358244115 1102 18-19 0.3490177859867529 1102 20-21 0.40405363229749724 1102 22-23 0.320161749992522 1102 24-25 0.432635451349924 1102 26-27 0.4121095531149379 1102 28-29 0.4245959064750693 1102 30-31 0.38633080968805444 1102 32-33 0.273188699541592 1102 34-35 0.44112764072965405 1102 36-37 0.5645212914479387 1102 38-39 0.5696959325289228 1102 40-41 0.7034125147275745 1102 42-43 0.7280578468925114 1102 44-45 0.6959402499144645 1102 46-47 0.583638757005815 1102 48-49 0.48277993081001824 1102 50-51 0.32583234048778564 1102 52-53 0.3845386155186148 1102 54-55 0.355178644279853 1102 56-57 0.4649781717706958 1102 58-59 0.49480652924391677 1102 60-61 0.6425916330914916 1102 62-63 0.5635449160938251 1102 64-65 0.4079757519081326 1102 66-67 0.3917756091318907 1102 68-69 0.28700444008342885 1102 70-71 0.6266584053862729 1102 72-73 0.5357085435815883 1102 74-75 0.5630159074732148 1102 76-77 0.5663326324363105 1102 78-79 0.6846036454601041 1102 80-81 0.7901325358896969 1102 82-83 0.764564959145865 1102 84-85 0.7758349708533281 1102 86-87 0.6847425217417751 1102 88-89 0.7574769077583383 1102 90-91 0.9994449164843076 1102 92-93 1.2163670050556412 1102 94-95 1.2204431728164415 1102 96-97 1.3927034593105745 1102 98-99 1.588949850192158 1102 100-101 1.8884212798667406 1103 1 0.10912742657961161 1103 2 0.1970405726105895 1103 3 0.12019063347909764 1103 4 0.1290144222394929 1103 5 0.21177304855966383 1103 6 0.20427433975304155 1103 7 0.11389632407468042 1103 8 0.05938814425569916 1103 9 0.23587287771989907 1103 10-11 0.17236408245836543 1103 12-13 0.16098166362902333 1103 14-15 0.28393193826669005 1103 16-17 0.22351858559157733 1103 18-19 0.14068445265341722 1103 20-21 0.1888709838956757 1103 22-23 0.17575535729845626 1103 24-25 0.11242997189786763 1103 26-27 0.10760042069484399 1103 28-29 0.2182602900367172 1103 30-31 0.14175318411727744 1103 32-33 -0.033603537901328195 1103 34-35 0.015899330227377106 1103 36-37 0.17525188505523914 1103 38-39 0.22237858093074436 1103 40-41 0.29559288002437256 1103 42-43 0.3302267966642063 1103 44-45 0.33007267000579077 1103 46-47 0.4086958346313807 1103 48-49 0.35692376642645485 1103 50-51 0.12063827656084669 1103 52-53 0.16393359268756313 1103 54-55 0.34176540227073104 1103 56-57 0.5851265735971722 1103 58-59 0.4480028762758792 1103 60-61 0.6037788477033601 1103 62-63 0.37233487043172175 1103 64-65 0.4901675327300481 1103 66-67 0.5929742392688766 1103 68-69 0.5752464492158538 1103 70-71 0.6711789533314843 1103 72-73 0.8073980412984874 1103 74-75 0.7151277796193369 1103 76-77 0.5591979292399607 1103 78-79 0.6300945130400137 1103 80-81 0.7590252299536218 1103 82-83 0.5451014888262335 1103 84-85 0.698494788204929 1103 86-87 0.8576877272212293 1103 88-89 0.8781960858405284 1103 90-91 0.8955636379454965 1103 92-93 1.1096318452382867 1103 94-95 1.3137650906246634 1103 96-97 1.3068016328265557 1103 98-99 1.4593836401464948 1103 100-101 1.8570285858028157 1104 1 0.1257604950570439 1104 2 0.2011597892029826 1104 3 0.24698941372729166 1104 4 0.20087328626703282 1104 5 0.2661441533310054 1104 6 0.19720791357867995 1104 7 0.1984802566739603 1104 8 0.2383947774420676 1104 9 0.32684243108010946 1104 10-11 0.353430323940934 1104 12-13 0.16211388331009147 1104 14-15 0.39115584367304734 1104 16-17 0.3668300899259407 1104 18-19 0.3986262000736289 1104 20-21 0.5291749590919608 1104 22-23 0.5005909030037827 1104 24-25 0.4048157577025293 1104 26-27 0.33799705616038267 1104 28-29 0.26643537786356575 1104 30-31 0.34768503284289665 1104 32-33 0.3256367500606956 1104 34-35 0.36117131837566063 1104 36-37 0.3473389613447395 1104 38-39 0.5383803571157202 1104 40-41 0.6098002967755249 1104 42-43 0.3964014758113166 1104 44-45 0.33532312387606567 1104 46-47 0.3768870928148047 1104 48-49 0.3135884879347941 1104 50-51 0.4060499820913819 1104 52-53 0.3078325741319361 1104 54-55 0.3713578424816646 1104 56-57 0.40918454717446195 1104 58-59 0.5814107740116299 1104 60-61 0.5241796289103817 1104 62-63 0.44017275818627155 1104 64-65 0.6424434662095635 1104 66-67 0.5420877186266182 1104 68-69 0.5160542007700144 1104 70-71 0.5493123283187131 1104 72-73 0.4849369641130288 1104 74-75 0.3295723485015216 1104 76-77 0.4484447403591929 1104 78-79 0.32588796080366933 1104 80-81 0.49049366730380584 1104 82-83 0.476425119945624 1104 84-85 0.43118042311246185 1104 86-87 0.36116150491517374 1104 88-89 0.50716106513147 1104 90-91 0.6811285983742934 1104 92-93 0.7226750159642457 1104 94-95 0.7119625772443321 1104 96-97 0.8448668093840581 1104 98-99 1.194165232268407 1104 100-101 1.225743170167668 1105 1 0.1981426239598889 1105 2 0.2603476307281909 1105 3 0.27540343178284843 1105 4 0.30781033659816615 1105 5 0.2704573907913215 1105 6 0.29276887617643865 1105 7 0.2827327477393311 1105 8 0.2019580971405972 1105 9 0.3119415107898078 1105 10-11 0.3362752485505567 1105 12-13 0.3341002545886269 1105 14-15 0.32141767726890436 1105 16-17 0.35162422056922793 1105 18-19 0.4141464847648777 1105 20-21 0.5379013369495453 1105 22-23 0.4894697283480909 1105 24-25 0.442002639753845 1105 26-27 0.4335333388447893 1105 28-29 0.4937336609867984 1105 30-31 0.4873733424412592 1105 32-33 0.5506019361692154 1105 34-35 0.5807850202578564 1105 36-37 0.4861683761501894 1105 38-39 0.5371504184199551 1105 40-41 0.6725976601493855 1105 42-43 0.6650225549591227 1105 44-45 0.7117812249900126 1105 46-47 0.796678219608367 1105 48-49 0.7652694435951375 1105 50-51 0.5569306323969556 1105 52-53 0.745310915817555 1105 54-55 0.8913548267991089 1105 56-57 1.0740166172681143 1105 58-59 1.09470935937928 1105 60-61 1.0538031317609082 1105 62-63 1.0653080328483497 1105 64-65 1.1308689249263253 1105 66-67 1.2557951453534741 1105 68-69 1.1580091744656258 1105 70-71 1.1597703232752181 1105 72-73 1.1577070071608837 1105 74-75 1.0943413326796616 1105 76-77 1.2202432738314108 1105 78-79 1.1797074816685083 1105 80-81 1.2663402136880144 1105 82-83 1.059102660649728 1105 84-85 1.381786112136762 1105 86-87 1.216295315686505 1105 88-89 1.1203703633057316 1105 90-91 1.2898990619949195 1105 92-93 1.3197663022306152 1105 94-95 1.408226038362141 1105 96-97 1.4704314469223405 1105 98-99 1.5534278573118918 1105 100-101 1.553867689458741 1106 1 -0.01693453144477175 1106 2 0.16605422934888736 1106 3 0.04320810237440753 1106 4 0.08268414701013427 1106 5 0.10290140514296553 1106 6 0.12469137297326682 1106 7 0.13627389518607202 1106 8 0.08453267108742324 1106 9 0.18038609459392774 1106 10-11 -0.004223094450857445 1106 12-13 -0.06185933591016379 1106 14-15 -0.09797235983560881 1106 16-17 -0.01697353874338603 1106 18-19 -0.10169961169244601 1106 20-21 -0.0041273194849935635 1106 22-23 -0.19277759062999422 1106 24-25 -0.06911772195659438 1106 26-27 -0.08349599336405333 1106 28-29 0.16969717998686917 1106 30-31 0.25258484177719964 1106 32-33 0.14989841006901372 1106 34-35 -6.034346337528973E-4 1106 36-37 0.006640913583581209 1106 38-39 -0.06980230801399756 1106 40-41 -0.256929532107506 1106 42-43 -0.236163383889604 1106 44-45 -0.34242272189232636 1106 46-47 -0.14497650837896003 1106 48-49 0.02332494358702064 1106 50-51 0.06017579069933987 1106 52-53 0.051155011982942256 1106 54-55 -0.01937520767439338 1106 56-57 0.021719615363338107 1106 58-59 -0.19579556115866836 1106 60-61 -0.23392066216216278 1106 62-63 -0.0028096563999966406 1106 64-65 0.17115858882321788 1106 66-67 0.30029064830075214 1106 68-69 0.37265491555576347 1106 70-71 0.2379215489495934 1106 72-73 0.12137629943596906 1106 74-75 0.4540877100788947 1106 76-77 0.48209914829236666 1106 78-79 0.27993165853035507 1106 80-81 0.01581013632537065 1106 82-83 0.04593461270038901 1106 84-85 0.08957968200912703 1106 86-87 0.14191545123782134 1106 88-89 0.4147205297264165 1106 90-91 0.4675002974905489 1106 92-93 0.6043995550116534 1106 94-95 0.44419741436476556 1106 96-97 0.2994737035399737 1106 98-99 0.6415953195901736 1106 100-101 0.5807006146243978 1107 1 0.4723257257088065 1107 2 0.37346984727915356 1107 3 0.3179338393319924 1107 4 0.3151978175306951 1107 5 0.34836256494879336 1107 6 0.374333836281707 1107 7 0.4151707040716275 1107 8 0.3994116542962729 1107 9 0.476632243542916 1107 10-11 0.5002304826213262 1107 12-13 0.48497569700140275 1107 14-15 0.6982231996610011 1107 16-17 0.6279857054842921 1107 18-19 0.5653547179688942 1107 20-21 0.6281299932487627 1107 22-23 0.6593704716234541 1107 24-25 0.6210849769054221 1107 26-27 0.6383194290294512 1107 28-29 0.5571868063070227 1107 30-31 0.5632120373025842 1107 32-33 0.7360511106362964 1107 34-35 0.8541309423201895 1107 36-37 0.8509226002018195 1107 38-39 0.8981097686545993 1107 40-41 1.000574759469643 1107 42-43 0.9537818203439912 1107 44-45 0.6952050306854645 1107 46-47 0.8814630173307094 1107 48-49 1.0099522755334078 1107 50-51 0.9154007148151564 1107 52-53 0.895249688153612 1107 54-55 0.9484697953382337 1107 56-57 1.0082616841877297 1107 58-59 0.99248417009197 1107 60-61 1.1420675374217524 1107 62-63 1.0824972339971097 1107 64-65 1.0599072672448244 1107 66-67 1.2711073553252987 1107 68-69 1.2827193744759953 1107 70-71 1.213408969796994 1107 72-73 1.2340225256901967 1107 74-75 1.2960729766114127 1107 76-77 1.0911125971288094 1107 78-79 1.1733260402590382 1107 80-81 1.2738282803177334 1107 82-83 1.2690102240369754 1107 84-85 1.2672202808972948 1107 86-87 1.2179769771066518 1107 88-89 1.385043279064206 1107 90-91 1.5125664048311371 1107 92-93 1.518362812290281 1107 94-95 1.6230748969426685 1107 96-97 1.641991580374551 1107 98-99 1.8067232711152457 1107 100-101 1.774686153998907 1108 1 0.1110779209786088 1108 2 0.09432959071117608 1108 3 0.038113829169120095 1108 4 0.14640051336659354 1108 5 0.12115102563006275 1108 6 0.05761769246813486 1108 7 0.039362800208841975 1108 8 0.056238609089312774 1108 9 0.020181047622330084 1108 10-11 0.12565282133028433 1108 12-13 0.16013371185345449 1108 14-15 0.08927875670235608 1108 16-17 0.14051991603407288 1108 18-19 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-0.4135639676253007 2316 48-49 -0.3101650859830656 2316 50-51 -0.08063346954278217 2316 52-53 -0.5424845740357114 2316 54-55 -0.7433557343759389 2316 56-57 -0.3553452366598151 2316 58-59 -0.1347573222221783 2316 60-61 -0.13471835973842872 2316 62-63 -0.006247811933640435 2316 64-65 0.01965449450218415 2316 66-67 0.017039770043616898 2316 68-69 0.007074065352306036 2316 70-71 -0.09571791070112567 2316 72-73 0.015509668152329681 2316 74-75 0.14865357304550386 2316 76-77 0.06418396644891189 2316 78-79 0.2694535454563294 2316 80-81 0.34561812025136973 2316 82-83 0.45710996084404343 2316 84-85 0.5479796744270473 2316 86-87 0.7616086298968057 2316 88-89 0.7109775115547095 2316 90-91 0.6776969259941019 2316 92-93 0.7396650540386212 2316 94-95 0.6598559808469346 2316 96-97 0.9249169390645235 2316 98-99 1.1900267776233804 2316 100-101 1.5814780933313983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5612.0 3 910.0 4 843.0 5 923.0 6 1102.0 7 1351.0 8 1503.0 9 1942.0 10 2387.0 11 3160.0 12 4251.0 13 5828.0 14 7007.0 15 8822.0 16 11285.0 17 15077.0 18 13215.0 19 9095.0 20 8677.0 21 8746.0 22 9630.0 23 10424.0 24 11980.0 25 13535.0 26 15781.0 27 18817.0 28 22761.0 29 27885.0 30 34467.0 31 44201.0 32 58002.0 33 77883.0 34 109271.0 35 160279.0 36 228863.0 37 301967.0 38 219806.0 39 25034.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.982866704012146 19.109690884920948 15.14247923687027 22.76496317419663 2 15.027677998160586 19.712714793145842 41.42903099642128 23.83057621227229 3 19.189519993379587 24.35377563741494 29.653109057952637 26.80359531125283 4 12.500767524247092 16.552828360221152 37.505038963535256 33.4413651519965 5 13.711538166326386 37.24453052885626 34.13968501025516 14.9042462945622 6 31.79212265651733 36.05076270118082 17.566906859674855 14.590207782626996 7 28.44068759729112 32.84808379008194 20.358804988241697 18.35242362438524 8 26.17509018890148 35.128352029175254 19.494075111064575 19.202482670858696 9 26.042359388454923 17.065818936392883 20.60392777839662 36.28789389675558 10-11 24.775298473663813 26.872234795502436 27.04645977117297 21.30600695966078 12-13 25.718799336477527 24.157940759208977 27.91229871050664 22.21096119380686 14-15 22.56681024995231 25.392297194723213 26.601004771731922 25.439887783592553 16-17 21.725945943062015 28.94613113022323 26.994690074266934 22.33323285244782 18-19 21.5691466213767 27.135063898269646 29.86700129487243 21.42878818548122 20-21 22.355281101934253 26.22152052789346 30.133773911419798 21.28942445875249 22-23 21.958007538955272 26.195135159649247 30.071688101735884 21.7751691996596 24-25 21.72576570423982 26.281242413652357 30.318846688417533 21.674145193690293 26-27 21.57028298866765 26.701086550568753 29.937329267772157 21.79130119299144 28-29 21.328925769772837 26.714117343375747 30.224326842715747 21.732630044135668 30-31 21.57725514756961 26.404186555439612 30.230101138850063 21.788457158140716 32-33 21.33708912170982 26.516661919681 30.115721949817136 22.03052700879204 34-35 21.876367308390073 26.61302371246238 29.852410731436617 21.658198247710924 36-37 21.79649328256508 26.412752039271925 29.85974930868323 21.931005369479763 38-39 21.52774536332563 26.675464134205168 29.917968838560917 21.87882166390829 40-41 21.802952064951832 26.59285615218018 29.88008903137703 21.72410275149097 42-43 21.724402273167573 26.528691188057834 29.716600972261638 22.030305566512958 44-45 21.685942697088173 26.78395118332097 29.544927850348866 21.98517826924199 46-47 21.80466747227713 26.97821383889391 29.1387904105679 22.078328278261054 48-49 21.992249380517396 27.203240319229472 28.732019996865066 22.07249030338806 50-51 21.99518377008555 27.22540536268292 28.665838950100564 22.113571917130965 52-53 22.12099925216861 27.278400748231658 28.594377188540903 22.00622281105883 54-55 21.99670076498636 27.161033947040842 28.65982832907118 22.182436958901615 56-57 21.98575672681434 27.53627247119556 28.47504426643409 22.002926535556007 58-59 22.047986503899967 27.227148232054237 28.5362499312359 22.188615332809896 60-61 21.991925060615387 27.18016195884121 28.847089211378258 21.98082376916515 62-63 21.902388821118628 27.22408604330103 28.853650575943497 22.019874559636847 64-65 21.850578441200895 27.229793230732714 28.73543061956102 22.184197708505373 66-67 21.754160315675914 27.427512025209268 28.54715335592352 22.271174303191298 68-69 21.834625279923614 27.217893507505803 28.725021912138743 22.22245930043184 70-71 21.803110514999638 27.34988109443133 28.572409707281928 22.27459868328711 72-73 21.917099879346118 27.30155036407868 28.54512885361473 22.236220902960472 74-75 21.80540203497435 27.519602007965844 28.395778003404402 22.2792179536554 76-77 21.846072494055296 27.489474238724398 28.343337863267127 22.32111540395318 78-79 21.961024641093207 27.479471868449245 28.266254375783756 22.29324911467379 80-81 21.788741922115577 27.6546954667161 28.293758103722986 22.26280450744534 82-83 21.94204363327991 27.673878433767392 28.2795295328663 22.1045484000864 84-85 21.95158456367539 27.533783502243903 28.186702872185208 22.327929061895503 86-87 21.987447363999777 27.463891503836198 28.277232713124135 22.2714284190399 88-89 22.04165559608933 27.479345386344534 28.157848153127667 22.32115086443846 90-91 21.95493805407687 27.53292163851559 28.171534247205592 22.34060606020195 92-93 22.06822288453717 27.468712286386758 28.17347762020016 22.28958720887591 94-95 21.84433248177847 27.424055985926177 28.16195166639941 22.569659865895943 96-97 22.105967846846948 27.36398621483822 27.978364083899187 22.55168185441565 98-99 22.01999705258862 27.280792947017947 27.965665991607214 22.733544008786218 100-101 22.150355536738797 26.884944777627023 28.403568368731435 22.561131316902745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2046.0 1 1235.0 2 511.5 3 596.5 4 446.0 5 352.5 6 687.5 7 1615.5 8 2748.0 9 3141.5 10 2510.0 11 1830.5 12 1762.5 13 1913.0 14 2019.5 15 2147.0 16 2282.5 17 2351.0 18 2475.5 19 2679.0 20 2941.0 21 3452.0 22 4258.0 23 5293.0 24 6439.0 25 7821.0 26 9720.5 27 11925.5 28 13862.0 29 15846.0 30 18417.5 31 21548.5 32 24674.0 33 27223.0 34 30144.0 35 33131.0 36 36241.0 37 39320.0 38 41371.0 39 43019.0 40 45170.5 41 48093.0 42 50689.0 43 52896.0 44 54782.0 45 56685.0 46 57222.5 47 57346.0 48 58788.5 49 57951.0 50 55367.5 51 52081.0 52 48783.5 53 47455.5 54 45877.5 55 42828.0 56 39267.5 57 35254.0 58 31555.0 59 27603.0 60 23149.5 61 18822.5 62 14992.0 63 11727.5 64 8978.5 65 7028.0 66 5357.5 67 4199.0 68 3080.0 69 2044.5 70 1497.5 71 1076.5 72 843.5 73 727.5 74 570.0 75 455.0 76 340.5 77 224.0 78 156.5 79 109.0 80 75.5 81 54.0 82 35.5 83 26.0 84 18.5 85 9.5 86 10.0 87 14.5 88 11.5 89 6.0 90 6.0 91 4.5 92 3.0 93 3.5 94 2.0 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3065234581969898 2 0.26824962791180645 3 0.2619926886999678 4 0.2667852378835038 5 0.2703130865880511 6 0.20774369446966448 7 0.19742640108844117 8 0.19829172246880183 9 0.20721118900482718 10-11 0.20774369446966448 12-13 0.20374990348338448 14-15 0.20541398306100117 16-17 0.19143571460902117 18-19 0.19679405084894686 20-21 0.18551159131270584 22-23 0.1943644946656265 24-25 0.19489700013046385 26-27 0.1935657364683705 28-29 0.20574679897652448 30-31 0.20345036915941347 32-33 0.20867557903312983 34-35 0.20208582390576785 36-37 0.18893959524259618 38-39 0.17692494069220385 40-41 0.17546055066390118 42-43 0.18893959524259618 44-45 0.18933897434122418 46-47 0.1895719454820905 48-49 0.2061461780751525 50-51 0.20145347366627347 52-53 0.22172196292164448 54-55 0.19260057031335284 56-57 0.17349693676231354 58-59 0.17569352180476752 60-61 0.16684061845184686 62-63 0.17143347808606885 64-65 0.1709675358043362 66-67 0.17080112784657453 68-69 0.17256505219884818 70-71 0.1738963158609415 72-73 0.17223223628332485 74-75 0.18667644701703753 76-77 0.17825620435429718 78-79 0.18261609284765284 80-81 0.17542726907234885 82-83 0.15815412305668786 84-85 0.1581874046482402 86-87 0.16028414491603718 88-89 0.16544279160664888 90-91 0.17283130493126686 92-93 0.16920361145206253 94-95 0.16817188211394019 96-97 0.17329724721299952 98-99 0.1804527893967512 100-101 0.16554263638130587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1502332.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.78690984855283 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01481843054715 48.63853554485993 2 11.910886552922657 14.480519742176993 3 3.482379416358511 6.350492509219224 4 1.4695680152939126 3.5732199384795105 5 0.7970071000864153 2.422379937080472 6 0.46933526864469677 1.71176643983109 7 0.31512676319413846 1.3408907501603846 8 0.2316201721254739 1.1263579617677977 9 0.17060608419190482 0.9333554993449138 >10 1.0350085070651762 11.532506105484673 >50 0.07307126898109585 3.043171852476216 >100 0.029476673478326793 3.142261913604142 >500 4.382988442459987E-4 0.1589244948598218 >1k 4.382988442459987E-4 0.6083224692182533 >5k 2.1914942212299934E-4 0.9372948414364677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7927 0.5276463524706922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6145 0.4090307601781763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4329 0.28815201966010173 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2017 0.13425794032211255 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1589 0.10576889795331525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.3281591552333304E-5 0.0 0.0 0.0 0.0 12-13 1.3312636620933322E-4 0.0 0.0 0.0 0.0 14-15 1.3312636620933322E-4 0.0 0.0 0.0 0.0 16-17 1.3312636620933322E-4 0.0 0.0 6.656318310466661E-5 0.0 18-19 1.3312636620933322E-4 0.0 0.0 6.656318310466661E-5 0.0 20-21 1.9968954931399982E-4 0.0 0.0 1.664079577616665E-4 0.0 22-23 1.9968954931399982E-4 0.0 0.0 2.995343239709997E-4 0.0 24-25 1.9968954931399982E-4 0.0 0.0 3.3281591552333307E-4 0.0 26-27 2.6625273241866643E-4 0.0 0.0 3.993790986279997E-4 0.0 28-29 2.6625273241866643E-4 0.0 0.0 7.321950141513328E-4 0.0 30-31 2.6625273241866643E-4 0.0 0.0 0.0017972059438259983 0.0 32-33 2.6625273241866643E-4 0.0 0.0 0.0031950327890239976 0.0 34-35 3.6609750707566636E-4 0.0 0.0 0.006323502394943328 0.0 36-37 3.9937909862799965E-4 0.0 0.0 0.01218106250815399 0.0 38-39 4.659422817326663E-4 0.0 0.0 0.01973598379053365 0.0 40-41 4.659422817326663E-4 0.0 0.0 0.028655450326558977 0.0 42-43 4.659422817326663E-4 0.0 0.0 0.0360772452427293 0.0 44-45 4.659422817326663E-4 0.0 0.0 0.04522968291962096 0.0 46-47 5.325054648373329E-4 0.0 0.0 0.059108106596943946 0.0 48-49 5.325054648373329E-4 0.0 0.0 0.0751498337251686 0.0 50-51 5.325054648373329E-4 0.0 0.0 0.09235641655772492 0.0 52-53 5.657870563896663E-4 0.0 0.0 0.11076113668616525 0.0 54-55 5.990686479419996E-4 0.0 0.0 0.1331263662093332 0.0 56-57 5.990686479419996E-4 0.0 0.0 0.15818740464824022 0.0 58-59 5.990686479419996E-4 0.0 0.0 0.1881741186368925 0.0 60-61 5.990686479419996E-4 0.0 0.0 0.22268712907666216 0.0 62-63 5.990686479419996E-4 0.0 0.0 0.25766608179816447 0.0 64-65 5.990686479419996E-4 0.0 0.0 0.2977038364356214 0.0 66-67 6.656318310466661E-4 0.0 0.0 0.34396524869336476 0.0 68-69 6.656318310466661E-4 0.0 0.0 0.4019750627690817 0.0 70-71 6.656318310466661E-4 0.0 0.0 0.47160015229656294 0.0 72-73 6.656318310466661E-4 0.0 0.0 0.5441207402890973 0.0 74-75 6.656318310466661E-4 0.0 0.0 0.6197365162959985 0.0 76-77 6.656318310466661E-4 0.0 0.0 0.7067013150222454 0.0 78-79 6.656318310466661E-4 0.0 0.0 0.8102736279331066 0.0 80-81 6.656318310466661E-4 0.0 0.0 0.9221996203236036 0.0 82-83 6.656318310466661E-4 0.0 0.0 1.0381194037003805 0.0 84-85 7.321950141513327E-4 0.0 0.0 1.1635577222611246 0.0 86-87 7.987581972559993E-4 0.0 0.0 1.2996128685270634 0.0 88-89 7.987581972559993E-4 0.0 0.0 1.4543722692454133 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3255 0.0 54.343567 1 GTATCAA 5935 0.0 43.985325 1 ATCAACG 7640 0.0 34.15554 3 TCAACGC 7780 0.0 33.6065 4 TATCAAC 7830 0.0 33.328957 2 CAACGCA 7990 0.0 32.77834 5 AACGCAG 8165 0.0 32.11579 6 ACGCAGA 9325 0.0 28.019737 7 CGCAGAG 9380 0.0 27.90609 8 CGTAATA 70 0.008362492 27.16271 2 TGGTATC 1535 0.0 25.702826 2 GCAGAGT 10240 0.0 25.14404 9 GTGGTAT 1725 0.0 23.43074 1 GAGTACT 6240 0.0 23.17824 12-13 GTACATG 6180 0.0 23.15977 1 TACATGG 6275 0.0 22.574268 2 AGAGTAC 9565 0.0 21.75147 10-11 ACATGGG 6260 0.0 21.563843 3 AGTACTT 6660 0.0 21.431274 12-13 CAGAGTA 9930 0.0 21.406382 10-11 >>END_MODULE