FastQCFastQC Report
Thu 26 May 2016
SRR937570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502332
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81950.5454852855427429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73050.48624405257958964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52420.3489242058346624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19070.12693599018059923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.053.5907021
GTATCAA70950.040.2619671
ATCAACG93750.030.2036843
TATCAAC95050.029.7905882
TCAACGC95050.029.7905884
CAACGCA98500.028.747165
AACGCAG100750.028.1429526
AACGCGT350.00838127.15127894-95
GTACGAC908.9378515E-426.394443
TGGTATC18750.025.5920472
ACGCAGA116450.024.2703297
CGCAGAG117100.024.0139168
GTGGTAT20700.023.650471
GCAGAGT124500.022.2432069
GAGTACT78400.022.1143912-13
AGTACTT81950.021.2723412-13
AGAGTAC113200.021.21157510-11
GTACATG62450.021.00631
GTACTTT82600.020.98713714-15
CGTAGAC1604.9825197E-520.7856183
Produced by FastQC (version 0.11.5)