FastQCFastQC Report
Thu 26 May 2016
SRR937569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454773
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78510.5396718250888627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60110.4131916113373014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41680.2865051798459279No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19840.136378665262553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.10482735107126678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.053.880451
GTATCAA57150.041.843241
ATCAACG73200.032.520763
TATCAAC74700.031.9368442
TCAACGC74600.031.9104524
CAACGCA76700.031.1019295
AACGCAG78500.030.4440546
ACGCAGA90850.026.2009437
CGCAGAG91450.025.9251338
GGACCGA2151.6589183E-924.3085126
GCAGAGT99300.023.4434269
TGGTATC15400.023.1445872
GTACATG59150.022.8263841
GAGTACT59200.022.58609612-13
TACATGG60200.021.867152
GTGGTAT16400.021.4516851
CAGAGTA96200.021.0102610-11
GTACTTT64300.020.79394714-15
AGTACTT62700.020.7571412-13
AGAGTAC93100.020.51083210-11
Produced by FastQC (version 0.11.5)