##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937569_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1454773 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11063719219425 33.0 31.0 34.0 31.0 34.0 2 32.27215792429472 34.0 31.0 34.0 31.0 34.0 3 32.30113839066301 34.0 31.0 34.0 31.0 34.0 4 35.8800465777135 37.0 35.0 37.0 35.0 37.0 5 35.87684951535394 37.0 35.0 37.0 35.0 37.0 6 35.936923492531136 37.0 36.0 37.0 35.0 37.0 7 35.893163400750495 37.0 36.0 37.0 35.0 37.0 8 35.88326426184704 37.0 36.0 37.0 35.0 37.0 9 37.574812702737816 39.0 38.0 39.0 35.0 39.0 10-11 37.48134898021891 39.0 37.0 39.0 35.0 39.0 12-13 37.37233918968801 39.0 37.0 39.0 35.0 39.0 14-15 38.60871455546673 40.0 38.0 41.0 35.0 41.0 16-17 38.532056203957595 40.0 38.0 41.0 35.0 41.0 18-19 38.47122609506775 40.0 38.0 41.0 34.0 41.0 20-21 38.38642867306446 40.0 38.0 41.0 34.0 41.0 22-23 38.19759990046557 40.0 38.0 41.0 34.0 41.0 24-25 38.02812431905184 40.0 38.0 41.0 34.0 41.0 26-27 37.98011064269133 40.0 38.0 41.0 33.5 41.0 28-29 38.0241395049262 40.0 38.0 41.0 34.0 41.0 30-31 37.84587698561906 40.0 37.5 41.0 33.5 41.0 32-33 37.610572577302435 40.0 37.0 41.0 33.0 41.0 34-35 37.63240003766911 40.0 37.0 41.0 33.0 41.0 36-37 37.546439547613275 40.0 37.0 41.0 33.0 41.0 38-39 37.416444696182836 40.0 37.0 41.0 33.0 41.0 40-41 37.18365717538063 40.0 37.0 41.0 32.5 41.0 42-43 36.92543029049893 40.0 36.5 41.0 31.0 41.0 44-45 36.69203545845296 39.5 36.0 41.0 31.0 41.0 46-47 36.34232007330353 39.0 36.0 41.0 30.0 41.0 48-49 35.98385658793502 39.0 35.5 41.0 29.5 41.0 50-51 35.06738061539498 38.0 34.5 39.5 27.5 40.5 52-53 35.17751910435511 38.5 35.0 40.0 28.0 40.5 54-55 35.519568001330796 39.0 35.0 40.0 28.0 41.0 56-57 35.31768220883946 39.0 35.0 40.0 27.5 41.0 58-59 35.06969712800554 38.0 35.0 40.0 26.5 41.0 60-61 34.75759757707904 38.0 34.0 40.0 26.0 41.0 62-63 34.39994899547902 38.0 34.0 40.0 26.0 41.0 64-65 34.02899765117994 37.0 34.0 40.0 25.0 41.0 66-67 33.615589511215845 36.5 33.5 39.5 23.5 41.0 68-69 33.129094023603685 36.0 33.0 39.0 22.0 40.5 70-71 32.69115868936253 35.5 32.0 39.0 21.5 40.0 72-73 32.2308648840747 35.0 32.0 38.0 19.5 40.0 74-75 31.770410572646043 35.0 31.5 37.0 18.0 39.0 76-77 31.272365516819463 35.0 31.0 37.0 15.0 39.0 78-79 30.744799360450052 34.5 31.0 36.0 9.5 39.0 80-81 30.21658602407386 34.0 30.0 36.0 7.0 37.5 82-83 29.69808829281269 34.0 29.5 35.0 2.0 37.0 84-85 29.22752656256337 34.0 29.0 35.0 2.0 36.5 86-87 28.74884913316373 34.0 29.0 35.0 2.0 36.0 88-89 28.25914317903893 34.0 28.5 35.0 2.0 36.0 90-91 27.767962080682004 33.0 27.0 35.0 2.0 35.0 92-93 27.202643986381382 33.0 26.0 35.0 2.0 35.0 94-95 26.56402373428707 32.5 25.0 35.0 2.0 35.0 96-97 25.832788345673173 32.0 23.5 34.5 2.0 35.0 98-99 24.883575994330386 32.0 18.5 34.0 2.0 35.0 100-101 22.448631848405213 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09405022229931603 1101 2 0.14203614808462106 1101 3 0.11351418589368478 1101 4 0.05585402866372391 1101 5 0.010606528698936302 1101 6 0.0281995958549075 1101 7 0.06592473100884888 1101 8 0.06557314626578403 1101 9 0.019263224208010854 1101 10-11 0.13226140400065844 1101 12-13 0.20984266717617572 1101 14-15 0.26310269697365385 1101 16-17 0.2255571094158384 1101 18-19 0.2794766272500553 1101 20-21 0.36568556446735556 1101 22-23 0.27126893976535627 1101 24-25 0.28265381964756386 1101 26-27 0.21088835437349474 1101 28-29 0.0676408599372067 1101 30-31 0.12439585189142122 1101 32-33 0.25170395825676195 1101 34-35 0.31130094759165416 1101 36-37 0.19444076988130377 1101 38-39 0.18220897310416007 1101 40-41 0.2607921979173895 1101 42-43 0.31282440557316704 1101 44-45 0.47167012488288407 1101 46-47 0.496976919408354 1101 48-49 0.49722974416807375 1101 50-51 0.5289327679026385 1101 52-53 0.4862037637725791 1101 54-55 0.5373685874704108 1101 56-57 0.5912992155952068 1101 58-59 0.5531533538634719 1101 60-61 0.5524257531244885 1101 62-63 0.6762560241956734 1101 64-65 0.5590859609213297 1101 66-67 0.7125123313013688 1101 68-69 0.6586664508239082 1101 70-71 0.6868536385980164 1101 72-73 0.716338408438908 1101 74-75 0.7072751028955082 1101 76-77 0.8064113078040229 1101 78-79 0.7893769527432042 1101 80-81 0.817735611736051 1101 82-83 0.8144175717242348 1101 84-85 0.8234522682068324 1101 86-87 0.9038867186362687 1101 88-89 0.7516208858969655 1101 90-91 0.7737103314616895 1101 92-93 0.7642720488973929 1101 94-95 1.0082860781372602 1101 96-97 1.2352154855252486 1101 98-99 1.3368777111360437 1101 100-101 1.797533294857626 1102 1 0.2119570046344279 1102 2 0.10350759405656618 1102 3 0.20449603919308856 1102 4 0.10917490715191036 1102 5 0.11481451846452728 1102 6 0.09264386755937437 1102 7 0.018471375743388307 1102 8 0.14407457279789782 1102 9 0.12843525587827287 1102 10-11 0.08615174251465163 1102 12-13 0.20941661792902977 1102 14-15 0.22401156690067836 1102 16-17 0.16265333429341666 1102 18-19 0.3295623641028058 1102 20-21 0.377927686920529 1102 22-23 0.31475840661586574 1102 24-25 0.4106856532831529 1102 26-27 0.2491066351964406 1102 28-29 0.2545241425354874 1102 30-31 0.2875354939375967 1102 32-33 0.28329385481259806 1102 34-35 0.35567792097204887 1102 36-37 0.45039969524248846 1102 38-39 0.39117155389211433 1102 40-41 0.4935796107065684 1102 42-43 0.4827146307814445 1102 44-45 0.6056873987342328 1102 46-47 0.42648429646671104 1102 48-49 0.5989926032137802 1102 50-51 0.5223597189842124 1102 52-53 0.40106950638283934 1102 54-55 0.4352671287910965 1102 56-57 0.3772961774210657 1102 58-59 0.36774699807167366 1102 60-61 0.48743175108104 1102 62-63 0.6274869722021847 1102 64-65 0.5268758473323558 1102 66-67 0.4798001811739425 1102 68-69 0.4064337683177186 1102 70-71 0.4943058937440412 1102 72-73 0.6470812789074287 1102 74-75 0.7176717255947267 1102 76-77 0.7061311396636079 1102 78-79 0.7866757313471346 1102 80-81 0.7427837172579288 1102 82-83 0.7413162823447337 1102 84-85 0.8474202145473555 1102 86-87 1.066027524701024 1102 88-89 1.0399244753824561 1102 90-91 1.1555906068472765 1102 92-93 1.142528682387006 1102 94-95 1.2497837029991672 1102 96-97 1.5021691823547307 1102 98-99 1.7315658141019519 1102 100-101 2.3535446899869292 1103 1 0.23532843656776237 1103 2 0.1643909185974195 1103 3 0.18610723194483114 1103 4 0.19818995709029963 1103 5 0.2358771186801647 1103 6 0.16081601563326586 1103 7 0.21404711446650282 1103 8 0.19218977937578074 1103 9 0.19066459443313732 1103 10-11 0.23651216937759756 1103 12-13 0.35465725084491595 1103 14-15 0.4352874978232748 1103 16-17 0.39489373415157303 1103 18-19 0.3525715326653014 1103 20-21 0.29038330600084095 1103 22-23 0.37641046410603707 1103 24-25 0.4736336677580866 1103 26-27 0.32522549918792265 1103 28-29 0.3143920244828138 1103 30-31 0.2744927124049923 1103 32-33 0.2379997193836303 1103 34-35 0.246196372025544 1103 36-37 0.37527709615151394 1103 38-39 0.40059348946741835 1103 40-41 0.5184091974012901 1103 42-43 0.5011354550256968 1103 44-45 0.45310430975395377 1103 46-47 0.5851325746511762 1103 48-49 0.5678678575213709 1103 50-51 0.4770256029409623 1103 52-53 0.45400741518868415 1103 54-55 0.43295881714644224 1103 56-57 0.38040085089483 1103 58-59 0.3325253770755481 1103 60-61 0.5731828551798301 1103 62-63 0.8079927822305635 1103 64-65 0.7515660869592509 1103 66-67 0.6644530860581028 1103 68-69 0.5886857235485294 1103 70-71 0.6453164118960544 1103 72-73 0.833185512594433 1103 74-75 0.7983248138046406 1103 76-77 0.866263088692552 1103 78-79 1.0893616485170519 1103 80-81 0.9563632466633472 1103 82-83 1.0311048759079853 1103 84-85 0.9626516584228604 1103 86-87 1.1102337323577345 1103 88-89 1.1848277109193077 1103 90-91 1.3231971429951166 1103 92-93 1.3307278352530467 1103 94-95 1.3948492988354637 1103 96-97 1.7536787755893606 1103 98-99 1.820346993699097 1103 100-101 2.3168439487853476 1104 1 0.14879531799456203 1104 2 0.23977380360891942 1104 3 0.1691157774409362 1104 4 0.11992684643319507 1104 5 0.20368555317664772 1104 6 0.125607150678114 1104 7 0.12985966720975028 1104 8 0.1404482717515947 1104 9 0.26164000359342054 1104 10-11 0.27287275593283056 1104 12-13 0.17382874860916786 1104 14-15 0.28087821219564546 1104 16-17 0.35781677776650866 1104 18-19 0.3228437766606973 1104 20-21 0.34401200825038813 1104 22-23 0.3136423909749837 1104 24-25 0.3082291525670229 1104 26-27 0.36006738170393504 1104 28-29 0.26293991351268886 1104 30-31 0.2575222043873495 1104 32-33 0.311158422698945 1104 34-35 0.4930981353510262 1104 36-37 0.29004567601995745 1104 38-39 0.33037935940348717 1104 40-41 0.4364652026080762 1104 42-43 0.362964053112556 1104 44-45 0.37617423348193313 1104 46-47 0.3327691407143831 1104 48-49 0.125067014031508 1104 50-51 0.1039929807157094 1104 52-53 0.03244027617037659 1104 54-55 0.1343601203195135 1104 56-57 0.20099453128965905 1104 58-59 0.22197099564036193 1104 60-61 0.29671487515172146 1104 62-63 0.470150927196336 1104 64-65 0.28394566812337274 1104 66-67 0.3435616304147189 1104 68-69 0.3374835628095596 1104 70-71 0.3489789301530095 1104 72-73 0.2707802326052686 1104 74-75 0.41297063050646443 1104 76-77 0.34049522402093046 1104 78-79 0.340578220438573 1104 80-81 0.29464078861161624 1104 82-83 0.42645891174290185 1104 84-85 0.6127841742734894 1104 86-87 0.8270347246514369 1104 88-89 0.8381422493894561 1104 90-91 0.6416177511202079 1104 92-93 0.6841170102430105 1104 94-95 0.9394757040095456 1104 96-97 1.2882292505886426 1104 98-99 1.3094233173326977 1104 100-101 1.8347552053935452 1105 1 0.16752088576134128 1105 2 0.09482567485389382 1105 3 0.1594498992337634 1105 4 0.06977129483604472 1105 5 0.0814839975225965 1105 6 0.1488404697887944 1105 7 0.09637361300075042 1105 8 0.14285921794441236 1105 9 0.17067235252610402 1105 10-11 0.12733569902705 1105 12-13 0.20041060409876366 1105 14-15 0.2651963604343237 1105 16-17 0.29975557342570625 1105 18-19 0.3691574868804324 1105 20-21 0.3467426188439191 1105 22-23 0.3011892877447835 1105 24-25 0.3179212667293285 1105 26-27 0.3383319285054256 1105 28-29 0.33257151927741546 1105 30-31 0.3471632136707399 1105 32-33 0.2088306030587077 1105 34-35 0.40812332877215596 1105 36-37 0.30412437283864335 1105 38-39 0.38832802530516375 1105 40-41 0.4777989142915047 1105 42-43 0.42333885444423913 1105 44-45 0.31331771792056173 1105 46-47 0.35410656685671427 1105 48-49 0.3168566789983558 1105 50-51 0.3435227633577185 1105 52-53 0.3893435083019625 1105 54-55 0.47485655982423225 1105 56-57 0.45549020028580145 1105 58-59 0.34703088956425887 1105 60-61 0.3770783674058933 1105 62-63 0.43387592191462687 1105 64-65 0.44645635237088754 1105 66-67 0.5017927470860144 1105 68-69 0.6332169751269205 1105 70-71 0.6962294020549891 1105 72-73 0.8840232452109618 1105 74-75 0.7749919451487557 1105 76-77 0.7696657397783859 1105 78-79 0.7668734826393262 1105 80-81 0.9493259088206223 1105 82-83 0.9983395602404777 1105 84-85 1.0913794321936372 1105 86-87 1.0678718173209738 1105 88-89 1.0127207825786293 1105 90-91 1.181607905732836 1105 92-93 1.4580045021823587 1105 94-95 1.4662091066610365 1105 96-97 1.736286201943038 1105 98-99 2.0267057004411626 1105 100-101 2.2540119822098 1106 1 0.03201857721307988 1106 2 0.07818918501592975 1106 3 0.0941924028170007 1106 4 0.07736684018518503 1106 5 0.015522792486279968 1106 6 -0.12485334934556391 1106 7 -0.11167996902638322 1106 8 -0.02364926036708681 1106 9 -0.020394554887381844 1106 10-11 -0.26911583147659 1106 12-13 -0.2510368402201877 1106 14-15 -0.17843223835076572 1106 16-17 -0.3219261232216226 1106 18-19 -0.009943201885853625 1106 20-21 0.11853275173048416 1106 22-23 0.16172141834575626 1106 24-25 0.16550031708165136 1106 26-27 0.20317458743880934 1106 28-29 0.15053449568940636 1106 30-31 0.17615951481795378 1106 32-33 0.275897248355335 1106 34-35 0.21796256098681255 1106 36-37 0.09848472841601108 1106 38-39 0.0932750217715963 1106 40-41 0.18504506789364683 1106 42-43 0.2873830586983743 1106 44-45 0.36975527442385925 1106 46-47 0.3897438154979227 1106 48-49 0.33080417549025043 1106 50-51 0.09658364542267606 1106 52-53 0.284114444050779 1106 54-55 0.4124493754851244 1106 56-57 0.4869110405790025 1106 58-59 0.4039121247523596 1106 60-61 0.37138504746223333 1106 62-63 0.6211951426762781 1106 64-65 0.626674835949764 1106 66-67 0.5399249057351625 1106 68-69 0.46419543062423685 1106 70-71 0.5111630088580128 1106 72-73 0.5607392549576957 1106 74-75 0.7291999640879467 1106 76-77 0.6409738781727405 1106 78-79 0.8422667205253944 1106 80-81 0.8175255834284236 1106 82-83 0.6166817713213426 1106 84-85 0.6588198444349977 1106 86-87 0.7680967453014453 1106 88-89 0.8119757947286637 1106 90-91 1.1399317435910206 1106 92-93 1.1624996809852846 1106 94-95 1.268810282513897 1106 96-97 1.1327727926625997 1106 98-99 1.3625255961119755 1106 100-101 1.9911081532168353 1107 1 0.06138554762571147 1107 2 0.020296030971934442 1107 3 -0.08701670661776717 1107 4 0.038958993402694375 1107 5 -0.04301052037767761 1107 6 -0.07309806498592764 1107 7 0.0238473733681559 1107 8 -0.02498425648701641 1107 9 0.11811444708315122 1107 10-11 0.07906263908220268 1107 12-13 0.03902576663943336 1107 14-15 0.1892102989830775 1107 16-17 0.15730888278249466 1107 18-19 0.17988786264105983 1107 20-21 0.03973664567946855 1107 22-23 0.04041889283088551 1107 24-25 0.07763578511891467 1107 26-27 0.09971554267185923 1107 28-29 0.10291942377669017 1107 30-31 -0.0045757845572325095 1107 32-33 0.12244882383161126 1107 34-35 0.10103347266635154 1107 36-37 0.09201289939982615 1107 38-39 0.18645009595557127 1107 40-41 -0.0010438855812751058 1107 42-43 0.17856825681322874 1107 44-45 0.1466239640540863 1107 46-47 0.32815860224134497 1107 48-49 0.07060379989115262 1107 50-51 0.07236486420598709 1107 52-53 0.061517090068605285 1107 54-55 0.04448044968592768 1107 56-57 0.04690006282263681 1107 58-59 0.19002631444882212 1107 60-61 0.18170014271844792 1107 62-63 0.26251041248303153 1107 64-65 0.2898289009793018 1107 66-67 0.3522563750931269 1107 68-69 0.12745107144551326 1107 70-71 0.2880558832005491 1107 72-73 0.43187217203357875 1107 74-75 0.24601633061882922 1107 76-77 0.3813974681514374 1107 78-79 0.6022500646191737 1107 80-81 0.5151720954671539 1107 82-83 0.7083298437997705 1107 84-85 0.8912151614334825 1107 86-87 0.9646288678569519 1107 88-89 0.9837373040229558 1107 90-91 1.090168858922656 1107 92-93 1.1380695464131314 1107 94-95 1.2042204867800805 1107 96-97 1.220856459149104 1107 98-99 1.4029956627986913 1107 100-101 1.9987654537402797 1108 1 0.029902151380589714 1108 2 -0.007515701073423031 1108 3 -0.022738327263226665 1108 4 0.06824696887063908 1108 5 0.10397290065445475 1108 6 0.16360724580775354 1108 7 0.13614102152010332 1108 8 0.025502985285463353 1108 9 0.09309196172518597 1108 10-11 0.05929446843426689 1108 12-13 0.1305769376485486 1108 14-15 0.1992975492539628 1108 16-17 0.2793085924203922 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-0.007732238505461453 2316 72-73 0.004913493615582354 2316 74-75 -0.11765787993079613 2316 76-77 0.14109305549661144 2316 78-79 -0.21602650505471033 2316 80-81 -0.09212672201811856 2316 82-83 -0.24071868211664338 2316 84-85 1.570986317638301E-4 2316 86-87 -0.19150884122376155 2316 88-89 -0.1446961961320561 2316 90-91 0.0566303201624514 2316 92-93 0.073760527708437 2316 94-95 0.33203984695251165 2316 96-97 0.4504547584537697 2316 98-99 0.2587126269394133 2316 100-101 0.5339471122134896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5367.0 3 900.0 4 756.0 5 882.0 6 1014.0 7 1134.0 8 1353.0 9 1770.0 10 2197.0 11 2772.0 12 4106.0 13 5476.0 14 6455.0 15 8169.0 16 10629.0 17 14579.0 18 12432.0 19 8389.0 20 7867.0 21 8222.0 22 8794.0 23 9784.0 24 10910.0 25 12432.0 26 14283.0 27 16938.0 28 20690.0 29 25229.0 30 31764.0 31 40784.0 32 53551.0 33 72868.0 34 103548.0 35 152430.0 36 222982.0 37 299841.0 38 227164.0 39 26308.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93259337136554 19.113907033660226 15.114044914772236 22.839454680201996 2 15.036193674412706 19.685471386250125 41.42483557415671 23.853499365180458 3 19.243837285593777 24.24513295804778 29.740644969207104 26.77038478715134 4 12.538089955001826 16.512744888607124 37.559486483320356 33.38967867307069 5 13.652381595521538 37.306885554970606 34.10032441848607 14.940408431021796 6 31.709329709333844 36.088091841661715 17.599839647665696 14.602738801338749 7 28.407567067950573 32.802979221349894 20.448054063473062 18.34139964722647 8 26.111559224035265 35.11630998638211 19.562574435153852 19.209556354428774 9 25.95398656793525 17.0840020664715 20.5847425520923 36.37726881350095 10-11 24.786697178189314 26.913801274650545 27.04936722114608 21.25013432601406 12-13 25.706394584908043 24.123548575638353 27.950800446780217 22.219256392673383 14-15 22.492217395631823 25.428030325207647 26.64683145801182 25.43292082114871 16-17 21.673847900199508 28.993299084285102 27.02311208062451 22.309740934890876 18-19 21.546825659497777 27.100806547433525 29.92198335107561 21.43038444199309 20-21 22.264130541832905 26.183367665785802 30.25209042279537 21.300411369585923 22-23 21.92637091253679 26.182456396489957 30.130015109547664 21.761157581425586 24-25 21.65299819670545 26.31670287373598 30.35646832492634 21.673830604632233 26-27 21.545026363539627 26.624850498704983 30.047444384485132 21.78267875327026 28-29 21.326165430055255 26.668616038786308 30.24402625935122 21.76119227180722 30-31 21.50357290823262 26.420958122808578 30.274478011693528 21.80099095726527 32-33 21.29608869280404 26.5455372041722 30.1190113328546 22.039362770169156 34-35 21.838886493471072 26.59958425342633 29.88464341259335 21.676885840509254 36-37 21.75282951037797 26.373743300702884 29.960888914600233 21.912538274318912 38-39 21.526731068757204 26.66147372727357 29.935401624618663 21.87639357935056 40-41 21.804045259526056 26.582396165474115 29.908991990744248 21.70456658425558 42-43 21.75865516306809 26.54206386349963 29.72397768844616 21.975303284986122 44-45 21.66293153202046 26.770756857197558 29.561260570063734 22.00505104071825 46-47 21.796886272249214 26.974712971409225 29.103103018306953 22.125297738034607 48-49 21.996954285352192 27.186309299141246 28.753350837123833 22.06338557838273 50-51 21.926271712393827 27.2633687454495 28.69149441058906 22.11886513156762 52-53 22.066696679495028 27.29905556035868 28.645775028477168 21.988472731669127 54-55 21.94973955122779 27.205997312518555 28.687728017510643 22.156535118743015 56-57 21.937510565650953 27.576460883076674 28.48998729195351 21.99604125931886 58-59 22.05772884369472 27.173817985502026 28.582918503817993 22.185534666985262 60-61 21.920989665845227 27.182067127071917 28.877270678553142 22.01967252852971 62-63 21.844413131571915 27.235380974812877 28.900759773335206 22.019446120279998 64-65 21.854415227622756 27.182822992520407 28.809823775152783 22.15293800470406 66-67 21.769235457431886 27.381902161056765 28.610303558903528 22.23855882260782 68-69 21.80543427058684 27.223624077507292 28.749886367079785 22.22105528482608 70-71 21.76185445750741 27.28725470211664 28.66342265610366 22.28746818427229 72-73 21.8862273593423 27.25346035952342 28.572594379545606 22.287717901588678 74-75 21.792340499380543 27.52525861196257 28.4057892167024 22.276611671954488 76-77 21.837404891931335 27.463787131491745 28.40345175049814 22.295356226078773 78-79 21.960172101046858 27.432056902312024 28.303686093641677 22.30408490299944 80-81 21.773098516225033 27.677446393218446 28.325407722159028 22.22404736839749 82-83 21.933889573983116 27.65468082739792 28.279737001872313 22.13169259674665 84-85 21.95115904216104 27.51133836235171 28.200506942132087 22.33699565335516 86-87 21.977802126814098 27.39710791298533 28.32312120058043 22.30196875962015 88-89 22.043201261181782 27.437935248729055 28.186228173211386 22.332635316877777 90-91 21.87612024949646 27.52278034345881 28.279754773618777 22.321344633425955 92-93 22.02066686593397 27.43707583957421 28.25120214657717 22.291055147914648 94-95 21.857093466594673 27.42230630640558 28.183643896902446 22.5369563300973 96-97 21.98719016971889 27.353415518611506 28.09406775809377 22.565326553575833 98-99 21.934698119204185 27.303373146570266 27.97148762991354 22.790441104312013 100-101 22.111795767373575 26.901631238689173 28.385261006453888 22.60131198748337 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2101.0 1 1264.0 2 492.5 3 568.0 4 438.0 5 369.5 6 713.0 7 1604.0 8 2671.0 9 3029.5 10 2413.0 11 1772.5 12 1691.5 13 1843.5 14 1994.5 15 2057.0 16 2174.5 17 2327.5 18 2427.0 19 2543.5 20 2801.0 21 3356.0 22 4111.0 23 4987.0 24 6277.5 25 7942.5 26 9701.0 27 11557.0 28 13248.0 29 15309.5 30 18099.0 31 21050.5 32 23939.0 33 26565.5 34 29462.5 35 32085.0 36 35110.5 37 38026.0 38 39953.0 39 41582.5 40 43535.5 41 46500.5 42 48791.0 43 51125.5 44 53601.0 45 55243.5 46 55392.0 47 55193.5 48 56650.0 49 56051.5 50 53370.0 51 50658.0 52 47729.5 53 45880.0 54 44140.0 55 41393.0 56 38128.0 57 34196.0 58 30339.0 59 26704.5 60 22541.5 61 18228.5 62 14458.5 63 11132.0 64 8528.0 65 6745.5 66 5131.5 67 3935.5 68 2870.0 69 1935.0 70 1399.0 71 1047.5 72 824.5 73 706.0 74 575.5 75 460.0 76 335.0 77 207.0 78 131.5 79 94.5 80 63.5 81 45.5 82 31.5 83 21.0 84 17.0 85 11.0 86 12.0 87 11.5 88 6.5 89 7.5 90 6.5 91 2.5 92 3.5 93 4.5 94 4.0 95 3.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2920043195742566 2 0.27268859127850187 3 0.2604530053829704 4 0.24766750551460606 5 0.24478045715723346 6 0.20415556241420485 7 0.19590685282171172 8 0.20663017529195274 9 0.20779874248422264 10-11 0.211785618787261 12-13 0.20982655025904387 14-15 0.20467110676373562 16-17 0.20322758258504936 18-19 0.20398371429769455 20-21 0.19205745501188157 22-23 0.1863520975437405 24-25 0.18618024942723022 26-27 0.19676609340426304 28-29 0.20783311210752467 30-31 0.20240271162580004 32-33 0.20762689436771234 34-35 0.20119977481022813 36-37 0.18937662439432132 38-39 0.1925729993614124 40-41 0.1858021835709076 42-43 0.19793466059653295 44-45 0.19212619425848568 46-47 0.19133569292253844 48-49 0.19913759741210482 50-51 0.20690513227836918 52-53 0.21800652060493286 54-55 0.19631928830133638 56-57 0.1748382737375522 58-59 0.1766598637725611 60-61 0.18191841613777543 62-63 0.18978905987394595 64-65 0.1802686742192768 66-67 0.18439302901552337 68-69 0.18758940398261448 70-71 0.18291513521353503 72-73 0.18449613788542957 74-75 0.18518353035147064 76-77 0.19741911624700212 78-79 0.2028838863520288 80-81 0.194841394499348 82-83 0.1940852627867028 84-85 0.18789873059233295 86-87 0.1884830141884679 88-89 0.1925729993614124 90-91 0.19305417408764117 92-93 0.19157628028565282 94-95 0.19171375877886102 96-97 0.17920321589691313 98-99 0.18569907470100147 100-101 0.18044052233578708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1454773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.89676588574472 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09008101289837 48.772269132127995 2 11.76849063159792 14.33326037641997 3 3.5261196763037197 6.441878532389566 4 1.4976950679057015 3.6481914367395216 5 0.8001427759749978 2.436305365185966 6 0.4802895415208833 1.7548847860421337 7 0.3183609553218746 1.3571006794378762 8 0.23479442295559513 1.1438576804804321 9 0.17178549471369475 0.9415062948732036 >10 1.0123564882231646 11.468719877132349 >50 0.06884344400135761 2.8608280813178975 >100 0.029911776805984322 3.1179451596154553 >500 4.514847107085506E-4 0.1631079101598624 >1k 4.514847107085506E-4 0.6073415483238703 >5k 2.257423553542753E-4 0.9528031397539034 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7851 0.5396718250888627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6011 0.4131916113373014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4168 0.2865051798459279 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1984 0.136378665262553 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.10482735107126678 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.873924660410937E-5 0.0 18-19 0.0 0.0 0.0 6.873924660410937E-5 0.0 20-21 0.0 0.0 0.0 1.3747849320821875E-4 0.0 22-23 0.0 0.0 0.0 1.3747849320821875E-4 0.0 24-25 0.0 0.0 0.0 2.0621773981232812E-4 0.0 26-27 0.0 0.0 0.0 3.7806585632260155E-4 0.0 28-29 0.0 0.0 0.0 8.248709592493125E-4 0.0 30-31 0.0 0.0 0.0 0.002508982501049992 0.0 32-33 0.0 0.0 0.0 0.005017965002099984 0.0 34-35 0.0 0.0 0.0 0.007148881646827375 0.0 36-37 0.0 0.0 0.0 0.01151382380618832 0.0 38-39 0.0 0.0 0.0 0.018593966206411586 0.0 40-41 0.0 0.0 0.0 0.026292761826071834 0.0 42-43 3.4369623302054687E-5 0.0 0.0 0.0345071017952629 0.0 44-45 6.873924660410937E-5 0.0 0.0 0.04464614066936903 0.0 46-47 2.405873631143828E-4 0.0 0.0 0.05839398999019091 0.0 48-49 3.4369623302054687E-4 0.0 0.0 0.0737915812295114 0.0 50-51 3.4369623302054687E-4 0.0 0.0 0.0913200891135593 0.0 52-53 3.7806585632260155E-4 0.0 0.0 0.10912355398402362 0.0 54-55 4.1243547962465624E-4 0.0 0.0 0.12988280645846464 0.0 56-57 4.1243547962465624E-4 0.0 0.0 0.1569316999971817 0.0 58-59 4.1243547962465624E-4 0.0 0.0 0.1881049483321453 0.0 60-61 4.1243547962465624E-4 0.0 0.0 0.22591153396440544 0.0 62-63 4.1243547962465624E-4 0.0 0.0 0.2639243373364779 0.0 64-65 4.1243547962465624E-4 0.0 0.0 0.30527099416884973 0.0 66-67 4.1243547962465624E-4 0.0 0.0 0.35816584443071187 0.0 68-69 4.1243547962465624E-4 0.0 0.0 0.4185532725724219 0.0 70-71 4.468051029267109E-4 0.0 0.0 0.4882548686289888 0.0 72-73 4.811747262287656E-4 0.0 0.0 0.5588500748914091 0.0 74-75 4.811747262287656E-4 0.0 0.0 0.6331915700937534 0.0 76-77 4.811747262287656E-4 0.0 0.0 0.7217964589664505 0.0 78-79 4.811747262287656E-4 0.0 0.0 0.825249025105635 0.0 80-81 4.811747262287656E-4 0.0 0.0 0.9424838101889436 0.0 82-83 4.811747262287656E-4 0.0 0.0 1.0645303425345398 0.0 84-85 5.842835961349297E-4 0.0 0.0 1.194756845226025 0.0 86-87 6.186532194369844E-4 0.0 0.0 1.3353973437780327 0.0 88-89 6.186532194369844E-4 0.0 0.0 1.4949411351461706 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3300 0.0 53.88045 1 GTATCAA 5715 0.0 41.84324 1 ATCAACG 7320 0.0 32.52076 3 TATCAAC 7470 0.0 31.936844 2 TCAACGC 7460 0.0 31.910452 4 CAACGCA 7670 0.0 31.101929 5 AACGCAG 7850 0.0 30.444054 6 ACGCAGA 9085 0.0 26.200943 7 CGCAGAG 9145 0.0 25.925133 8 GGACCGA 215 1.6589183E-9 24.308512 6 GCAGAGT 9930 0.0 23.443426 9 TGGTATC 1540 0.0 23.144587 2 GTACATG 5915 0.0 22.826384 1 GAGTACT 5920 0.0 22.586096 12-13 TACATGG 6020 0.0 21.86715 2 GTGGTAT 1640 0.0 21.451685 1 CAGAGTA 9620 0.0 21.01026 10-11 GTACTTT 6430 0.0 20.793947 14-15 AGTACTT 6270 0.0 20.75714 12-13 AGAGTAC 9310 0.0 20.510832 10-11 >>END_MODULE