FastQCFastQC Report
Thu 26 May 2016
SRR937569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454773
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.5356849487858243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70430.4841305138327423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49010.33689104760674No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.13039835080799547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.054.2735631
GTATCAA66050.041.009731
ATCAACG90300.030.0894323
TCAACGC91600.029.7661154
TATCAAC91350.029.6915782
CAACGCA93450.029.2276745
AACGCAG94900.028.879226
ACGCAGA109650.025.0420387
CGCAGAG111750.024.571458
TGGTATC16200.023.1642252
GAGTACT72600.022.96788412-13
GCAGAGT117950.022.9576479
TAGCGCG1503.033276E-522.1672844
ATACGTC2606.2573235E-1021.9236895
AGTACTT76450.021.81122812-13
GTGGTAT18700.021.600421
GTACTTT76850.021.53945714-15
AGAGTAC109150.021.47899410-11
CAGAGTA114950.020.39523510-11
GTACATG60350.020.315491
Produced by FastQC (version 0.11.5)