##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937569_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1454773 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.503991344354066 31.0 31.0 34.0 30.0 34.0 2 31.763262034695448 31.0 31.0 34.0 30.0 34.0 3 31.803324642401254 31.0 31.0 34.0 30.0 34.0 4 35.58213480728609 37.0 35.0 37.0 33.0 37.0 5 35.45312292708209 37.0 35.0 37.0 33.0 37.0 6 35.52509910480879 37.0 35.0 37.0 33.0 37.0 7 35.46644665525137 37.0 35.0 37.0 33.0 37.0 8 35.41613846283922 37.0 35.0 37.0 33.0 37.0 9 36.98000169098547 39.0 37.0 39.0 33.0 39.0 10-11 36.85258593608762 39.0 37.0 39.0 33.0 39.0 12-13 36.70259311933889 38.0 35.0 39.0 32.0 39.0 14-15 37.65503449679091 39.0 37.0 41.0 32.0 41.0 16-17 37.57517392747872 39.0 36.0 40.0 32.0 41.0 18-19 37.530790027035145 39.0 36.0 40.0 32.0 41.0 20-21 37.3843266269033 39.0 36.0 40.0 32.0 41.0 22-23 37.3093963800538 39.0 36.0 40.0 32.0 41.0 24-25 37.31648477116361 39.0 36.0 40.0 32.0 41.0 26-27 37.048853669954006 39.0 36.0 40.0 31.0 41.0 28-29 36.96577541650828 39.0 36.0 40.0 31.0 41.0 30-31 36.938128147827875 39.0 36.0 40.0 31.0 41.0 32-33 36.75414342993717 38.5 35.0 40.0 30.5 41.0 34-35 36.524659517326754 38.0 35.0 40.0 30.0 41.0 36-37 36.264560862760035 38.0 35.0 40.0 30.0 41.0 38-39 36.05471162854961 38.0 35.0 40.0 30.0 41.0 40-41 35.787650375694355 38.0 35.0 40.0 29.0 41.0 42-43 35.478308643341606 38.0 34.5 40.0 28.0 41.0 44-45 35.05233703127567 38.0 34.0 40.0 26.5 41.0 46-47 34.60228949808665 38.0 33.0 40.0 25.0 41.0 48-49 34.14737453884558 38.0 33.0 40.0 24.0 41.0 50-51 33.79205621770544 37.5 33.0 40.0 23.0 41.0 52-53 33.41372949594198 37.0 32.0 40.0 22.0 41.0 54-55 33.08376461482307 37.0 32.0 39.0 20.5 40.0 56-57 32.68981518078766 36.0 31.0 39.0 19.0 40.0 58-59 32.32946686527727 36.0 31.0 39.0 18.0 40.0 60-61 31.842613933582765 35.5 30.5 39.0 15.0 40.0 62-63 31.277235692441362 35.0 29.5 38.0 11.0 40.0 64-65 30.73863929286562 34.5 29.0 38.0 8.0 40.0 66-67 30.19231110283185 34.0 28.0 38.0 7.5 39.5 68-69 29.571227607331178 34.0 27.5 37.0 6.5 39.0 70-71 29.040192868578124 33.5 26.5 36.0 2.0 39.0 72-73 28.423005857271203 33.0 26.0 36.0 2.0 39.0 74-75 27.80141266025696 32.0 26.0 35.0 2.0 38.0 76-77 26.89641236124124 31.0 25.0 34.0 2.0 36.0 78-79 27.480279397541747 32.0 26.0 35.0 2.0 37.0 80-81 27.21399489817312 32.0 25.5 35.0 2.0 36.5 82-83 26.646907455664902 32.0 25.0 34.5 2.0 36.0 84-85 26.018819430935274 31.0 23.5 34.0 2.0 35.5 86-87 25.39592225041295 31.0 21.0 34.0 2.0 35.0 88-89 24.702071044760935 31.0 19.0 34.0 2.0 35.0 90-91 23.84233347745662 30.0 14.0 34.0 2.0 35.0 92-93 22.995587627760482 29.5 4.5 34.0 2.0 35.0 94-95 22.08401963742797 29.0 2.0 34.0 2.0 35.0 96-97 21.0948615350986 29.0 2.0 34.0 2.0 35.0 98-99 19.75012355879577 27.5 2.0 33.0 2.0 34.0 100-101 17.300045780338237 21.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24859684635839585 1101 2 0.1506953910921638 1101 3 0.2227037211053826 1101 4 0.16131119109900283 1101 5 0.14180917966588424 1101 6 -0.006244564140956754 1101 7 0.13030550436338473 1101 8 0.16694946996433657 1101 9 0.15416043399668666 1101 10-11 0.2335276983503647 1101 12-13 0.21259189639364706 1101 14-15 0.35092111266477843 1101 16-17 0.3680956500382919 1101 18-19 0.3995145516361376 1101 20-21 0.2898517697915963 1101 22-23 0.15974127390948212 1101 24-25 0.19034241477253033 1101 26-27 0.22783904294796287 1101 28-29 0.2808243379425832 1101 30-31 0.41604364001856453 1101 32-33 0.2748439978090573 1101 34-35 0.26821131550874355 1101 36-37 0.2200521258834769 1101 38-39 0.3334180825839539 1101 40-41 0.3382733227416921 1101 42-43 0.487623577668721 1101 44-45 0.6984459757756127 1101 46-47 0.627579065433487 1101 48-49 0.44565052328762533 1101 50-51 0.4357442835288765 1101 52-53 0.5165112658799842 1101 54-55 0.712601181933934 1101 56-57 0.7875717603724652 1101 58-59 0.732272947528152 1101 60-61 0.7134290182542777 1101 62-63 0.69284431041185 1101 64-65 0.8962132469970605 1101 66-67 0.9138902316690611 1101 68-69 1.0621245085533566 1101 70-71 0.903221525399104 1101 72-73 1.0475711280125353 1101 74-75 1.07128739641524 1101 76-77 1.1156000019800878 1101 78-79 1.061754163298712 1101 80-81 1.1092678986752382 1101 82-83 1.2902454298757604 1101 84-85 1.5192410009550734 1101 86-87 1.641643142897376 1101 88-89 1.786520454915557 1101 90-91 1.8033792586042345 1101 92-93 1.7419033756753208 1101 94-95 1.5692819837124148 1101 96-97 1.9336802369285238 1101 98-99 1.9228509507385283 1101 100-101 1.981280165365785 1102 1 0.223277959942763 1102 2 0.224805233076804 1102 3 0.253446459823067 1102 4 0.1786301671051973 1102 5 0.1931350212078442 1102 6 0.18801939996484407 1102 7 0.135549846159293 1102 8 0.10069263621345925 1102 9 0.24639095578925918 1102 10-11 0.23633333227936504 1102 12-13 0.15856153968486808 1102 14-15 0.05267346458177968 1102 16-17 0.12895340064319782 1102 18-19 0.30396703800486335 1102 20-21 0.2400626369953187 1102 22-23 0.34693581784410554 1102 24-25 0.3228730451936599 1102 26-27 0.24220087157043935 1102 28-29 0.39412824033630045 1102 30-31 0.29256050379544973 1102 32-33 0.26791159850127144 1102 34-35 0.18869590164974426 1102 36-37 0.2776835621585647 1102 38-39 0.2458882657241972 1102 40-41 0.4868515336576422 1102 42-43 0.3025436345587309 1102 44-45 0.2798777054597963 1102 46-47 0.3601770864042635 1102 48-49 0.3499966208609706 1102 50-51 0.5066754720465241 1102 52-53 0.6117193564349677 1102 54-55 0.24113429454070712 1102 56-57 0.647644473655653 1102 58-59 0.6214596298907296 1102 60-61 0.7041644645635081 1102 62-63 0.780226566341792 1102 64-65 0.7561774305338709 1102 66-67 0.6724398724174883 1102 68-69 0.4978139639415353 1102 70-71 0.5455117632554654 1102 72-73 0.5364281368602626 1102 74-75 0.6044708192622821 1102 76-77 0.4982151176210863 1102 78-79 0.5087946114623385 1102 80-81 0.597878533470503 1102 82-83 0.7042157609063118 1102 84-85 0.8478384409046917 1102 86-87 0.7435321533630059 1102 88-89 0.9060260335750847 1102 90-91 0.7959692625369961 1102 92-93 1.119534851475656 1102 94-95 1.5997482103752247 1102 96-97 1.6122902261447187 1102 98-99 1.502111920806719 1102 100-101 1.6193929137737406 1103 1 0.14487825066358795 1103 2 0.26392480144219377 1103 3 0.16841744462360353 1103 4 0.15707801564433055 1103 5 0.13320668077064113 1103 6 0.18623489937635185 1103 7 0.016477175379499442 1103 8 0.1361170856917795 1103 9 0.1618075781684567 1103 10-11 0.26100673454187984 1103 12-13 0.27686401480993084 1103 14-15 0.34192540778590796 1103 16-17 0.272032480022915 1103 18-19 0.21849222270392232 1103 20-21 0.40266395778579067 1103 22-23 0.34046497167553014 1103 24-25 0.2260388597531744 1103 26-27 0.25837660554788044 1103 28-29 0.3597167956091738 1103 30-31 0.36460719595219615 1103 32-33 0.22893960085850296 1103 34-35 0.500353403654465 1103 36-37 0.5029600216053822 1103 38-39 0.5763327558917268 1103 40-41 0.4766385995063658 1103 42-43 0.517384666110587 1103 44-45 0.6613595625495776 1103 46-47 0.759606338757024 1103 48-49 0.5082426550603216 1103 50-51 0.8052136442693651 1103 52-53 0.4264396010253151 1103 54-55 0.48307373138612775 1103 56-57 0.5139861921914743 1103 58-59 0.5242722823574582 1103 60-61 0.714740312176918 1103 62-63 0.44006304887985337 1103 64-65 0.4453161278354436 1103 66-67 0.5933112482804468 1103 68-69 0.7831198530210095 1103 70-71 0.9314044897415812 1103 72-73 1.0130784829851152 1103 74-75 1.1662507822135808 1103 76-77 0.9912771808613172 1103 78-79 0.9815596869980716 1103 80-81 1.2969200713784623 1103 82-83 1.3275307649157462 1103 84-85 1.3560926500020045 1103 86-87 1.3525103691289537 1103 88-89 1.5607609247221745 1103 90-91 1.5084611892158009 1103 92-93 1.5569117201224394 1103 94-95 1.6026009734744875 1103 96-97 1.8952431098034062 1103 98-99 2.0836642477922354 1103 100-101 2.0938228731007804 1104 1 0.13151841416013 1104 2 0.09111628986264009 1104 3 0.1904373964031194 1104 4 0.21246253615747435 1104 5 0.15432040376894207 1104 6 0.10681085066521234 1104 7 0.12759677679970594 1104 8 0.10202559009925238 1104 9 0.10919134527838281 1104 10-11 0.13959537035252367 1104 12-13 0.22894750314726764 1104 14-15 0.35869851611710857 1104 16-17 0.32048337844284447 1104 18-19 0.3297138754051545 1104 20-21 0.24136126731855967 1104 22-23 0.2635637177685908 1104 24-25 0.16814042574600307 1104 26-27 0.1954800047771883 1104 28-29 0.3921154799687514 1104 30-31 0.2996831428841915 1104 32-33 0.3081468115439634 1104 34-35 0.30910712458828726 1104 36-37 0.20912857747753577 1104 38-39 0.18813403976052 1104 40-41 0.36746651859193236 1104 42-43 0.5531074740524176 1104 44-45 0.5037184854510954 1104 46-47 0.38552253806569325 1104 48-49 0.454418228843231 1104 50-51 0.360051377705382 1104 52-53 0.6114627628119536 1104 54-55 0.7103933787880621 1104 56-57 0.627994900567451 1104 58-59 0.5785536771186202 1104 60-61 0.5438391038495318 1104 62-63 0.695693296812518 1104 64-65 0.8706610075422851 1104 66-67 0.8737595634121185 1104 68-69 0.9298186532733048 1104 70-71 0.8355233732838094 1104 72-73 0.5493564565985736 1104 74-75 0.9927074678963059 1104 76-77 0.8046848758965659 1104 78-79 0.9766377654445044 1104 80-81 1.0641184771665273 1104 82-83 0.9576407859158564 1104 84-85 0.9672487820942344 1104 86-87 1.0801941502478947 1104 88-89 0.8863871241179773 1104 90-91 0.9985404306111505 1104 92-93 1.2945948510624419 1104 94-95 1.2081270252059397 1104 96-97 1.2078285295743427 1104 98-99 1.2435923460456664 1104 100-101 1.6001510579553617 1105 1 0.13609382665148217 1105 2 0.14321675423904523 1105 3 0.16495054683041843 1105 4 0.08944421379491274 1105 5 0.21182202280353835 1105 6 0.09789062591973874 1105 7 0.14498776295402394 1105 8 0.11673498547095562 1105 9 0.02824513865228795 1105 10-11 0.19804597150110936 1105 12-13 0.19525604902635507 1105 14-15 0.13282661938183082 1105 16-17 0.25065327696493966 1105 18-19 0.26794097383278626 1105 20-21 0.35162166607745604 1105 22-23 0.2911131967087073 1105 24-25 0.16824157225724434 1105 26-27 0.13591541116522166 1105 28-29 0.2446687216971739 1105 30-31 0.16079310329551078 1105 32-33 0.04029773180460694 1105 34-35 0.3045337833565469 1105 36-37 0.23796154695959615 1105 38-39 0.35833185886222907 1105 40-41 0.5749193939835706 1105 42-43 0.6345621749142012 1105 44-45 0.721009498807959 1105 46-47 0.7609549135104956 1105 48-49 0.86563920876997 1105 50-51 0.6342219417922266 1105 52-53 0.5687975764054869 1105 54-55 0.6110858008776248 1105 56-57 0.5040420949082218 1105 58-59 0.47924402794857457 1105 60-61 0.6944369384166471 1105 62-63 0.4797727669014584 1105 64-65 0.4798239839147378 1105 66-67 0.5440150325731317 1105 68-69 0.6300283337884132 1105 70-71 0.5623556440609612 1105 72-73 0.7495523287162484 1105 74-75 1.185845522873059 1105 76-77 0.9584800448906385 1105 78-79 1.096328954444182 1105 80-81 0.8979399373493244 1105 82-83 0.9067395808570105 1105 84-85 1.1184293310332656 1105 86-87 1.2742905080068994 1105 88-89 0.9881232539279594 1105 90-91 1.134900426579101 1105 92-93 1.0606008549536448 1105 94-95 1.5311886368944698 1105 96-97 1.6008037835452917 1105 98-99 1.7895522160810273 1105 100-101 2.0470111664617505 1106 1 -0.13718553064467542 1106 2 -0.09920585659506287 1106 3 -0.09744511772345277 1106 4 0.03208444839783908 1106 5 -0.06820475652328639 1106 6 0.05292798591887049 1106 7 0.05675330360342201 1106 8 -0.07115980716873338 1106 9 -0.3192959297994449 1106 10-11 -0.30175911039371783 1106 12-13 -0.34979862404129847 1106 14-15 -0.11162322322284268 1106 16-17 -0.03565554176456942 1106 18-19 -0.21477267018414636 1106 20-21 -0.060814818584951524 1106 22-23 -0.09590567486988988 1106 24-25 -0.1621957455026788 1106 26-27 -0.02949347025528226 1106 28-29 -0.21973388978805275 1106 30-31 -0.23193536618415322 1106 32-33 -0.35975560980961063 1106 34-35 -0.5531211139295422 1106 36-37 -0.3829849286121956 1106 38-39 -0.20934207946017835 1106 40-41 -0.4728311592582912 1106 42-43 -0.6747473976099485 1106 44-45 -0.5246088089689778 1106 46-47 -0.621579589498424 1106 48-49 -0.631451628075645 1106 50-51 -0.7218251941637774 1106 52-53 -0.5706260503401097 1106 54-55 -0.5428542161015315 1106 56-57 -0.7109225234905452 1106 58-59 -0.7953563481974548 1106 60-61 -0.6747149966985866 1106 62-63 -0.9682642366521463 1106 64-65 -1.3875046112808498 1106 66-67 -1.0235059346881386 1106 68-69 -0.8728451430747661 1106 70-71 -0.7373812373662219 1106 72-73 -0.8701497178821214 1106 74-75 -0.8617589461357973 1106 76-77 -0.7132511534425028 1106 78-79 -0.7499290770582583 1106 80-81 -0.8597380412455813 1106 82-83 -0.6405096947738862 1106 84-85 -0.2463542681316362 1106 86-87 -0.04925246872229749 1106 88-89 -0.2932155705030013 1106 90-91 -0.6172386639755949 1106 92-93 -0.3993662218340681 1106 94-95 -0.11156717120594095 1106 96-97 -0.32882681534163183 1106 98-99 -0.46443755301405787 1106 100-101 -0.07134106182366295 1107 1 0.025594828004095405 1107 2 0.06024079718711306 1107 3 -0.009400146776652463 1107 4 -0.015400026068355999 1107 5 -0.006407138401996804 1107 6 -1.6818301239851507E-4 1107 7 -0.11935220062679974 1107 8 -0.03302577339005097 1107 9 -0.03831457726415266 1107 10-11 -0.09521683025903371 1107 12-13 0.062265761971943334 1107 14-15 0.07230088304688564 1107 16-17 0.14225624502300604 1107 18-19 0.1658267741983721 1107 20-21 0.20539430136491177 1107 22-23 0.0022773171785956947 1107 24-25 -0.05403266903903159 1107 26-27 0.020335028589109072 1107 28-29 0.058238884534937085 1107 30-31 0.05301837792476505 1107 32-33 0.20607244319486284 1107 34-35 0.32149019568314685 1107 36-37 0.12163792657185013 1107 38-39 0.2720668167285325 1107 40-41 0.25172873155299413 1107 42-43 0.45014098468516295 1107 44-45 0.4256148470444998 1107 46-47 0.18827978165699477 1107 48-49 0.5031806696612904 1107 50-51 0.39680018884845225 1107 52-53 0.3671485775833503 1107 54-55 0.3631671463600057 1107 56-57 0.6842701866062555 1107 58-59 0.5094376926894313 1107 60-61 0.6551905567422729 1107 62-63 0.611027550850455 1107 64-65 0.6489646730830039 1107 66-67 0.9285050767165295 1107 68-69 0.8049356256626048 1107 70-71 0.614149865225329 1107 72-73 0.5288879408694882 1107 74-75 0.8803125691100355 1107 76-77 0.6575059542341464 1107 78-79 0.9202541168800025 1107 80-81 1.0684512388281107 1107 82-83 0.9208429246407626 1107 84-85 1.0682722252743524 1107 86-87 1.337099782664115 1107 88-89 1.3982348182399633 1107 90-91 1.4888464199453537 1107 92-93 1.4784036112072947 1107 94-95 1.694420122356508 1107 96-97 1.3018724172669316 1107 98-99 1.584307260416658 1107 100-101 1.8628096446853881 1108 1 0.1177260150185333 1108 2 -0.023889851595136236 1108 3 0.12836250699132634 1108 4 0.15275539555865691 1108 5 0.010162622863695958 1108 6 0.08127413761818758 1108 7 0.13534564573623697 1108 8 0.048273844890012185 1108 9 0.12594255608287597 1108 10-11 0.08563308086183241 1108 12-13 0.18947534675413635 1108 14-15 0.003021724672066739 1108 16-17 0.12118852691503434 1108 18-19 0.1091208260244727 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2316 62-63 -0.10577124449034514 2316 64-65 -0.20850908571942384 2316 66-67 -0.06582446253085195 2316 68-69 0.25403286941417136 2316 70-71 0.612824149589855 2316 72-73 0.4217751369366276 2316 74-75 0.5138319159957838 2316 76-77 0.6452423464570067 2316 78-79 0.784620711208067 2316 80-81 0.7330259911791153 2316 82-83 0.6600534004516199 2316 84-85 0.8524359762523979 2316 86-87 0.6506551512219225 2316 88-89 1.0083164575212997 2316 90-91 0.9313800426331049 2316 92-93 1.131275749034483 2316 94-95 1.3065213373497926 2316 96-97 1.2332836105586367 2316 98-99 1.127664657407962 2316 100-101 1.552289544887639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 16.0 4 28.0 5 74.0 6 150.0 7 356.0 8 872.0 9 1510.0 10 2418.0 11 3728.0 12 5397.0 13 7633.0 14 9866.0 15 13434.0 16 17070.0 17 21675.0 18 19783.0 19 15714.0 20 15130.0 21 15669.0 22 17197.0 23 19373.0 24 22356.0 25 26253.0 26 31450.0 27 37817.0 28 45329.0 29 54246.0 30 64391.0 31 76334.0 32 92463.0 33 112328.0 34 137207.0 35 166036.0 36 187797.0 37 160010.0 38 52185.0 39 1470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.748584559910555 21.829476084322685 16.59788299395318 20.82405636181358 2 17.04142158261117 22.17005677174377 38.40090515839928 22.387616487245776 3 20.58059917251695 25.268272094684185 30.705340283329424 23.445788449469436 4 14.574026325756664 18.715290976667838 36.35653122514647 30.35415147242903 5 13.704707878361248 37.89517608141514 34.11197606601644 14.288139974207173 6 31.512751473941297 37.524204807210474 16.657581629573823 14.305462089274409 7 27.97281775232287 33.53169188595059 20.320421124120394 18.17506923760614 8 26.327131449373887 36.169835431369705 18.958421691906572 18.54461142734983 9 25.85709248109499 17.04059671165192 20.40648266086874 36.69582814638435 10-11 25.053118252813327 27.57746397547934 26.6870157749697 20.682401996737635 12-13 26.015114451393945 24.650125575676917 27.7962714661884 21.538488506740734 14-15 22.584141993286924 26.065956681901575 26.491830684237332 24.858070640574166 16-17 21.542536189494857 29.58513115104556 27.048927908340335 21.823404751119245 18-19 21.553396990458303 27.342616339456395 30.19285483027249 20.91113183981281 20-21 22.201325433797393 26.374668418147486 30.680987840681233 20.743018307373887 22-23 21.96865857548228 26.386524988451637 30.582119838982862 21.062696597083214 24-25 21.79449989792222 26.55998564724531 30.713554623298617 20.931959831533856 26-27 21.624632241744344 26.8449778657648 30.470730567241333 21.059659325249523 28-29 21.413664606520292 26.850265096913496 30.706217077699204 21.029853218867007 30-31 21.592784578762462 26.591777548799712 30.80009733477319 21.01534053766464 32-33 21.490274764705397 26.647749612997774 30.58609871512512 21.27587690717171 34-35 21.899499097109995 26.727812517829243 30.357829022122353 21.01485936293841 36-37 21.935448836017553 26.50689783288544 30.400713240629006 21.156940090468 38-39 21.748960147046997 26.807068869163782 30.319919327620187 21.124051656169037 40-41 21.924569623281172 26.752198970010422 30.25855597990613 21.064675426802275 42-43 21.917086315911057 26.691651394974937 30.060877071217817 21.33038521789619 44-45 21.92448581325059 26.934786389354215 29.760106903276316 21.380620894118877 46-47 22.08035205492541 27.221429047693352 29.337944820257185 21.36027407712406 48-49 22.346269830413405 27.44039791775074 28.827796501584785 21.38553575025107 50-51 22.21353434522087 27.513467736890913 28.732248948805072 21.540748969083147 52-53 22.498775581749037 27.592733571744038 28.498889001544914 21.409601844962012 54-55 22.357646175726384 27.543334939540397 28.5085714403553 21.59044744437792 56-57 22.336027682669393 27.79952611163391 28.413333214185304 21.45111299151139 58-59 22.543001554194365 27.520032334941604 28.368996400125656 21.567969710738378 60-61 22.339292796883086 27.536907819982908 28.732730123531304 21.391069259602702 62-63 22.27158463897804 27.53188985498081 28.740841354630586 21.455684151410566 64-65 22.25467478431343 27.494014530101946 28.62749033698041 21.623820348604212 66-67 22.196246424699936 27.66764986702393 28.461038251328556 21.67506545694758 68-69 22.230959744235008 27.58217261387172 28.478704237705816 21.70816340418746 70-71 22.25807737702033 27.57942304400755 28.398244949555707 21.76425462941641 72-73 22.39961148577819 27.54625635752107 28.28193814430155 21.772194012399186 74-75 22.341458083151117 27.719616737456633 28.061869446298495 21.877055733093755 76-77 22.26488256243414 27.662769380515034 28.311255432978204 21.761092624072624 78-79 22.300317644058556 27.659607375171248 28.163225465416254 21.876849515353943 80-81 22.14950373700914 27.883387992490928 28.15184912010327 21.81525915039666 82-83 22.34287126388664 27.84353270842377 28.170800823910024 21.642795203779567 84-85 22.32058068305666 27.765395136480386 28.029010781433072 21.88501339902988 86-87 22.421092500341977 27.609496464396848 28.088437165110985 21.88097387015019 88-89 22.44872567747683 27.62400044543032 28.024544035392463 21.90272984170039 90-91 22.366356849211478 27.75687121035047 28.009102033520783 21.86766990691727 92-93 22.434933958694153 27.61767017807729 28.034928734369558 21.912467128859 94-95 22.25405613109399 27.6153736699815 27.977457651468647 22.15311254745586 96-97 22.457902366898477 27.579319935137647 27.83929176579439 22.123485932169487 98-99 22.354763173985177 27.576377167395133 27.694437026321335 22.37442263229836 100-101 22.565828687633672 27.26339931154959 28.082781796144708 22.087990204672032 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 586.0 1 398.0 2 288.5 3 411.0 4 366.0 5 308.0 6 580.0 7 1312.5 8 2124.5 9 2500.5 10 2259.0 11 1834.0 12 1769.0 13 1937.5 14 2132.0 15 2325.0 16 2532.5 17 2644.5 18 2768.5 19 3070.0 20 3488.0 21 3962.5 22 4718.0 23 5692.5 24 6877.5 25 8456.0 26 10401.5 27 12323.5 28 14150.5 29 16214.0 30 18636.5 31 21848.0 32 24978.0 33 27543.0 34 30348.0 35 32920.5 36 35896.5 37 38796.5 38 40840.0 39 42537.5 40 44409.5 41 47223.5 42 49816.5 43 51949.5 44 53573.0 45 54951.0 46 55030.0 47 55024.0 48 56407.5 49 55472.0 50 53001.0 51 50051.5 52 46582.0 53 44783.5 54 43340.0 55 40302.0 56 36432.5 57 32627.0 58 29050.5 59 25506.5 60 21344.5 61 17279.0 62 13916.5 63 10828.5 64 8410.0 65 6526.0 66 4879.0 67 3715.0 68 2705.5 69 1801.5 70 1274.0 71 953.0 72 735.5 73 605.0 74 502.0 75 390.5 76 293.5 77 211.0 78 143.5 79 94.0 80 62.0 81 38.0 82 20.5 83 13.0 84 5.0 85 4.5 86 3.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03127635720486976 2 0.0 3 0.0 4 0.0 5 0.007217620893431484 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004983595378797929 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.7495698641643744E-4 22-23 0.0014435241786862967 24-25 0.0 26-27 8.936102058534219E-4 28-29 0.0015122634252904062 30-31 0.0 32-33 6.873924660410936E-5 34-35 0.0 36-37 3.093266097184921E-4 38-39 0.0 40-41 6.873924660410936E-5 42-43 0.019556315658869113 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.436962330205468E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7184811651027343E-4 84-85 2.4058736311438278E-4 86-87 0.0 88-89 0.0 90-91 0.010517104730428732 92-93 0.00271520024086232 94-95 0.0 96-97 0.0 98-99 1.7184811651027343E-4 100-101 0.013885327814030093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1454773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.68451026280051 #Duplication Level Percentage of deduplicated Percentage of total 1 80.71006539754147 49.78560857325947 2 11.459232515531411 14.137142914162295 3 3.392545391357337 6.2780250303059475 4 1.4288143978683432 3.525428655557877 5 0.7782756400846838 2.4003775854045655 6 0.4614027545938107 1.7076841770615807 7 0.3050636544866983 1.3172391488194521 8 0.21644093547645687 1.068084248455012 9 0.17176644619504008 0.9535796201810449 >10 0.9802824601912922 11.217697095538872 >50 0.06764137803276764 2.871621534784869 >100 0.02690607679901594 2.810483729983351 >500 0.0010047547553609117 0.35492623007351354 >1k 3.349182517869705E-4 0.5504317727934794 >5k 2.2327883452464704E-4 1.021669683618674 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7793 0.5356849487858243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7043 0.4841305138327423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4901 0.33689104760674 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1897 0.13039835080799547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.873924660410937E-5 0.0 0.0 0.0 0.0 3 6.873924660410937E-5 0.0 0.0 0.0 0.0 4 6.873924660410937E-5 0.0 0.0 0.0 0.0 5 6.873924660410937E-5 0.0 0.0 0.0 0.0 6 6.873924660410937E-5 0.0 0.0 0.0 0.0 7 6.873924660410937E-5 0.0 0.0 0.0 0.0 8 6.873924660410937E-5 0.0 0.0 0.0 0.0 9 6.873924660410937E-5 0.0 0.0 0.0 0.0 10-11 1.3747849320821875E-4 0.0 0.0 0.0 0.0 12-13 1.3747849320821875E-4 0.0 0.0 0.0 0.0 14-15 1.3747849320821875E-4 0.0 0.0 0.0 0.0 16-17 2.0621773981232812E-4 0.0 0.0 6.873924660410937E-5 0.0 18-19 3.7806585632260155E-4 0.0 0.0 6.873924660410937E-5 0.0 20-21 4.1243547962465624E-4 0.0 0.0 1.3747849320821875E-4 0.0 22-23 4.1243547962465624E-4 0.0 0.0 1.3747849320821875E-4 0.0 24-25 4.1243547962465624E-4 0.0 0.0 2.0621773981232812E-4 0.0 26-27 4.1243547962465624E-4 0.0 0.0 3.4369623302054687E-4 0.0 28-29 4.1243547962465624E-4 0.0 0.0 7.56131712645203E-4 0.0 30-31 4.1243547962465624E-4 0.0 0.0 0.002371504007841773 0.0 32-33 4.1243547962465624E-4 0.0 0.0 0.004743008015683546 0.0 34-35 4.1243547962465624E-4 0.0 0.0 0.006736446167202719 0.0 36-37 4.1243547962465624E-4 0.0 0.0 0.011101388326563663 0.0 38-39 4.1243547962465624E-4 0.0 0.0 0.018250269973391037 0.0 40-41 4.1243547962465624E-4 0.0 0.0 0.025674108606634848 0.0 42-43 4.811747262287656E-4 0.0 0.0 0.03361349158940948 0.0 44-45 4.811747262287656E-4 0.0 0.0 0.04354631272370328 0.0 46-47 4.811747262287656E-4 0.0 0.0 0.05677861769499434 0.0 48-49 4.811747262287656E-4 0.0 0.0 0.07172940383138812 0.0 50-51 4.811747262287656E-4 0.0 0.0 0.0887423673659052 0.0 52-53 4.811747262287656E-4 0.0 0.0 0.10613339675674487 0.0 54-55 4.811747262287656E-4 0.0 0.0 0.12623962638844685 0.0 56-57 4.811747262287656E-4 0.0 0.0 0.15249801859121664 0.0 58-59 4.811747262287656E-4 0.0 0.0 0.18219337312419187 0.0 60-61 4.811747262287656E-4 0.0 0.0 0.21835021683795341 0.0 62-63 5.49913972832875E-4 0.0 0.0 0.2550569745245478 0.0 64-65 5.49913972832875E-4 0.0 0.0 0.2947195198151189 0.0 66-67 5.49913972832875E-4 0.0 0.0 0.3452084964458373 0.0 68-69 5.49913972832875E-4 0.0 0.0 0.402846354723383 0.0 70-71 5.842835961349297E-4 0.0 0.0 0.4689735099565362 0.0 72-73 6.186532194369844E-4 0.0 0.0 0.536131753888751 0.0 74-75 6.186532194369844E-4 0.0 0.0 0.6068644386443796 0.0 76-77 6.873924660410937E-4 0.0 0.0 0.6918261474470587 0.0 78-79 7.561317126452031E-4 0.0 0.0 0.7903982270773515 0.0 80-81 7.561317126452031E-4 0.0 0.0 0.903164961131393 0.0 82-83 7.561317126452031E-4 0.0 0.0 1.0194717663855462 0.0 84-85 7.561317126452031E-4 0.0 0.0 1.1427899747933183 0.0 86-87 7.561317126452031E-4 0.0 0.0 1.2755254599858534 0.0 88-89 7.561317126452031E-4 0.0 0.0 1.4272329772411227 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3765 0.0 54.273563 1 GTATCAA 6605 0.0 41.00973 1 ATCAACG 9030 0.0 30.089432 3 TCAACGC 9160 0.0 29.766115 4 TATCAAC 9135 0.0 29.691578 2 CAACGCA 9345 0.0 29.227674 5 AACGCAG 9490 0.0 28.87922 6 ACGCAGA 10965 0.0 25.042038 7 CGCAGAG 11175 0.0 24.57145 8 TGGTATC 1620 0.0 23.164225 2 GAGTACT 7260 0.0 22.967884 12-13 GCAGAGT 11795 0.0 22.957647 9 TAGCGCG 150 3.033276E-5 22.167284 4 ATACGTC 260 6.2573235E-10 21.923689 5 AGTACTT 7645 0.0 21.811228 12-13 GTGGTAT 1870 0.0 21.60042 1 GTACTTT 7685 0.0 21.539457 14-15 AGAGTAC 10915 0.0 21.478994 10-11 CAGAGTA 11495 0.0 20.395235 10-11 GTACATG 6035 0.0 20.31549 1 >>END_MODULE