FastQCFastQC Report
Thu 26 May 2016
SRR937568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339876
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.5330344001982272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54470.4065301565219468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.295027301033827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18420.13747540817209952No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.10448728091256206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.048.904371
GTATCAA55200.042.044361
ATCAACG70200.032.499083
TCAACGC71400.031.952884
TATCAAC72600.031.555672
CAACGCA73050.031.2950445
AACGCAG74450.030.8169426
ACGCAGA85650.026.6217887
CGCAGAG87000.026.099498
GCAGAGT91200.024.7403559
TGGTATC15850.024.5896072
GTACATG55500.023.393581
GAGTACT57300.023.04264612-13
GTGGTAT17200.022.673181
TACATGG55250.022.4530432
TATCGTT1100.00287646621.5923887
ACATGGG56850.021.4866963
GTACTTT61250.021.47828714-15
AGAGTAC86300.021.38152310-11
AGTACTT60600.021.2784212-13
Produced by FastQC (version 0.11.5)